Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3942

Experiment: Gentamicin 0.001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3940 and Pf1N1B4_3941 are separated by 320 nucleotidesPf1N1B4_3941 and Pf1N1B4_3942 are separated by 53 nucleotidesPf1N1B4_3942 and Pf1N1B4_3943 are separated by 238 nucleotides Pf1N1B4_3940 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 4,375,892 to 4,376,620 _3940 Pf1N1B4_3941 - hypothetical protein, at 4,376,941 to 4,377,165 _3941 Pf1N1B4_3942 - Murein-DD-endopeptidase (EC 3.4.99.-), at 4,377,219 to 4,378,157 _3942 Pf1N1B4_3943 - Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) / Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9), at 4,378,396 to 4,379,250 _3943 Position (kb) 4377 4378 4379Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 4376.672 kb on + strandat 4376.673 kb on - strandat 4376.854 kb on + strandat 4376.854 kb on + strandat 4376.855 kb on - strandat 4376.855 kb on - strandat 4376.895 kb on + strandat 4376.940 kb on + strandat 4376.940 kb on + strandat 4376.940 kb on + strandat 4376.940 kb on + strandat 4376.940 kb on + strandat 4376.940 kb on + strandat 4376.941 kb on - strandat 4376.941 kb on - strandat 4376.941 kb on - strandat 4376.941 kb on - strandat 4377.220 kb on + strandat 4377.221 kb on - strandat 4377.277 kb on + strandat 4377.277 kb on + strandat 4377.277 kb on + strandat 4377.277 kb on + strandat 4377.278 kb on - strandat 4377.278 kb on - strandat 4377.278 kb on - strandat 4377.278 kb on - strandat 4377.278 kb on - strandat 4377.278 kb on - strandat 4377.278 kb on - strandat 4377.362 kb on - strandat 4377.362 kb on - strand, within Pf1N1B4_3942at 4377.362 kb on - strand, within Pf1N1B4_3942at 4377.482 kb on + strand, within Pf1N1B4_3942at 4377.526 kb on + strand, within Pf1N1B4_3942at 4377.526 kb on + strand, within Pf1N1B4_3942at 4377.526 kb on + strand, within Pf1N1B4_3942at 4377.526 kb on + strand, within Pf1N1B4_3942at 4377.527 kb on - strand, within Pf1N1B4_3942at 4377.527 kb on - strandat 4377.733 kb on + strand, within Pf1N1B4_3942at 4377.919 kb on - strand, within Pf1N1B4_3942at 4377.988 kb on + strandat 4377.988 kb on + strand, within Pf1N1B4_3942at 4377.988 kb on + strand, within Pf1N1B4_3942at 4377.988 kb on + strand, within Pf1N1B4_3942at 4378.184 kb on + strandat 4378.185 kb on - strandat 4378.228 kb on - strandat 4378.228 kb on - strandat 4378.233 kb on + strandat 4378.234 kb on - strandat 4378.292 kb on + strandat 4378.293 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml
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4,376,672 + -0.1
4,376,673 - +0.9
4,376,854 + -3.8
4,376,854 + -1.8
4,376,855 - +0.1
4,376,855 - +0.8
4,376,895 + -1.3
4,376,940 + -0.0
4,376,940 + -1.7
4,376,940 + +0.7
4,376,940 + -1.0
4,376,940 + +0.7
4,376,940 + -0.2
4,376,941 - -1.7
4,376,941 - -1.0
4,376,941 - +1.7
4,376,941 - -0.7
4,377,220 + +1.0
4,377,221 - -1.4
4,377,277 + -0.3
4,377,277 + +0.0
4,377,277 + -0.4
4,377,277 + +0.3
4,377,278 - +0.7
4,377,278 - +0.1
4,377,278 - -0.7
4,377,278 - +1.1
4,377,278 - -0.3
4,377,278 - -0.4
4,377,278 - -0.7
4,377,362 - -0.1
4,377,362 - Pf1N1B4_3942 0.15 -0.4
4,377,362 - Pf1N1B4_3942 0.15 -1.8
4,377,482 + Pf1N1B4_3942 0.28 -2.0
4,377,526 + Pf1N1B4_3942 0.33 -1.7
4,377,526 + Pf1N1B4_3942 0.33 -1.3
4,377,526 + Pf1N1B4_3942 0.33 -0.4
4,377,526 + Pf1N1B4_3942 0.33 -0.7
4,377,527 - Pf1N1B4_3942 0.33 -2.5
4,377,527 - -0.3
4,377,733 + Pf1N1B4_3942 0.55 +0.2
4,377,919 - Pf1N1B4_3942 0.75 -2.0
4,377,988 + -0.3
4,377,988 + Pf1N1B4_3942 0.82 -2.0
4,377,988 + Pf1N1B4_3942 0.82 -0.7
4,377,988 + Pf1N1B4_3942 0.82 -1.0
4,378,184 + -1.7
4,378,185 - +1.1
4,378,228 - +0.6
4,378,228 - -2.3
4,378,233 + -0.3
4,378,234 - +0.1
4,378,292 + +0.9
4,378,293 - +0.7

Or see this region's nucleotide sequence