Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3860 and Pf1N1B4_3861 are separated by 123 nucleotides Pf1N1B4_3861 and Pf1N1B4_3862 are separated by 144 nucleotides
Pf1N1B4_3860 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 4,288,162 to 4,289,871
_3860
Pf1N1B4_3861 - FIG00953368: hypothetical protein, at 4,289,995 to 4,290,738
_3861
Pf1N1B4_3862 - DNA polymerase III subunits gamma and tau (EC 2.7.7.7), at 4,290,883 to 4,292,970
_3862
Position (kb)
4289
4290
4291 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4289.025 kb on - strand, within Pf1N1B4_3860 at 4289.334 kb on + strand, within Pf1N1B4_3860 at 4289.335 kb on - strand, within Pf1N1B4_3860 at 4289.394 kb on - strand, within Pf1N1B4_3860 at 4289.394 kb on - strand, within Pf1N1B4_3860 at 4289.560 kb on + strand, within Pf1N1B4_3860 at 4289.560 kb on + strand, within Pf1N1B4_3860 at 4289.573 kb on + strand, within Pf1N1B4_3860 at 4289.574 kb on - strand, within Pf1N1B4_3860 at 4289.574 kb on - strand, within Pf1N1B4_3860 at 4289.574 kb on - strand, within Pf1N1B4_3860 at 4289.574 kb on - strand, within Pf1N1B4_3860 at 4289.574 kb on - strand, within Pf1N1B4_3860 at 4289.577 kb on + strand, within Pf1N1B4_3860 at 4289.598 kb on + strand, within Pf1N1B4_3860 at 4289.598 kb on + strand, within Pf1N1B4_3860 at 4289.599 kb on - strand, within Pf1N1B4_3860 at 4289.599 kb on - strand, within Pf1N1B4_3860 at 4289.764 kb on + strand at 4289.764 kb on + strand at 4289.909 kb on + strand at 4289.920 kb on + strand at 4290.018 kb on - strand at 4290.111 kb on + strand, within Pf1N1B4_3861 at 4290.111 kb on + strand, within Pf1N1B4_3861 at 4290.112 kb on - strand, within Pf1N1B4_3861 at 4290.112 kb on - strand, within Pf1N1B4_3861 at 4290.112 kb on - strand, within Pf1N1B4_3861 at 4290.112 kb on - strand at 4290.113 kb on + strand, within Pf1N1B4_3861 at 4290.114 kb on - strand at 4290.114 kb on - strand at 4290.114 kb on - strand, within Pf1N1B4_3861 at 4290.114 kb on - strand, within Pf1N1B4_3861 at 4290.114 kb on - strand, within Pf1N1B4_3861 at 4290.145 kb on + strand, within Pf1N1B4_3861 at 4290.218 kb on + strand, within Pf1N1B4_3861 at 4290.218 kb on + strand, within Pf1N1B4_3861 at 4290.321 kb on - strand, within Pf1N1B4_3861 at 4290.324 kb on + strand, within Pf1N1B4_3861 at 4290.324 kb on + strand at 4290.325 kb on - strand at 4290.325 kb on - strand at 4290.325 kb on - strand, within Pf1N1B4_3861 at 4290.325 kb on - strand, within Pf1N1B4_3861 at 4290.327 kb on - strand at 4290.327 kb on - strand at 4290.327 kb on - strand, within Pf1N1B4_3861 at 4290.443 kb on + strand, within Pf1N1B4_3861 at 4290.443 kb on + strand, within Pf1N1B4_3861 at 4290.771 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 4,289,025 - Pf1N1B4_3860 0.50 -1.2 4,289,334 + Pf1N1B4_3860 0.69 -2.4 4,289,335 - Pf1N1B4_3860 0.69 -0.2 4,289,394 - Pf1N1B4_3860 0.72 +0.2 4,289,394 - Pf1N1B4_3860 0.72 +0.4 4,289,560 + Pf1N1B4_3860 0.82 -0.0 4,289,560 + Pf1N1B4_3860 0.82 -2.8 4,289,573 + Pf1N1B4_3860 0.83 +0.4 4,289,574 - Pf1N1B4_3860 0.83 +0.2 4,289,574 - Pf1N1B4_3860 0.83 +1.0 4,289,574 - Pf1N1B4_3860 0.83 +0.1 4,289,574 - Pf1N1B4_3860 0.83 -1.3 4,289,574 - Pf1N1B4_3860 0.83 -0.5 4,289,577 + Pf1N1B4_3860 0.83 -0.1 4,289,598 + Pf1N1B4_3860 0.84 +0.8 4,289,598 + Pf1N1B4_3860 0.84 +0.7 4,289,599 - Pf1N1B4_3860 0.84 -1.2 4,289,599 - Pf1N1B4_3860 0.84 +0.4 4,289,764 + +0.9 4,289,764 + -0.6 4,289,909 + +0.4 4,289,920 + +2.1 4,290,018 - -0.6 4,290,111 + Pf1N1B4_3861 0.16 +1.1 4,290,111 + Pf1N1B4_3861 0.16 +0.9 4,290,112 - Pf1N1B4_3861 0.16 +1.4 4,290,112 - Pf1N1B4_3861 0.16 +1.2 4,290,112 - Pf1N1B4_3861 0.16 +1.1 4,290,112 - -0.6 4,290,113 + Pf1N1B4_3861 0.16 -2.8 4,290,114 - -1.2 4,290,114 - +3.4 4,290,114 - Pf1N1B4_3861 0.16 +1.9 4,290,114 - Pf1N1B4_3861 0.16 -0.1 4,290,114 - Pf1N1B4_3861 0.16 +0.4 4,290,145 + Pf1N1B4_3861 0.20 -0.2 4,290,218 + Pf1N1B4_3861 0.30 +0.6 4,290,218 + Pf1N1B4_3861 0.30 +1.4 4,290,321 - Pf1N1B4_3861 0.44 -0.1 4,290,324 + Pf1N1B4_3861 0.44 -0.1 4,290,324 + +0.4 4,290,325 - -1.0 4,290,325 - -0.6 4,290,325 - Pf1N1B4_3861 0.44 +1.1 4,290,325 - Pf1N1B4_3861 0.44 +2.4 4,290,327 - -0.4 4,290,327 - -1.6 4,290,327 - Pf1N1B4_3861 0.45 +0.7 4,290,443 + Pf1N1B4_3861 0.60 -0.1 4,290,443 + Pf1N1B4_3861 0.60 +0.0 4,290,771 + +1.9
Or see this region's nucleotide sequence