Experiment: Gentamicin 0.001 mg/ml 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt Pf1N1B4_3488 and Pf1N1B4_3489 are separated by 136 nucleotides Pf1N1B4_3489 and Pf1N1B4_3490 are separated by 158 nucleotides  
        
        Pf1N1B4_3488 - Alpha-methylacyl-CoA racemase (EC 5.1.99.4), at 3,895,845 to 3,897,026 
         
        _3488 
         
        
        Pf1N1B4_3489 - Protein sprT, at 3,897,163 to 3,897,657 
         
        _3489 
         
        
        Pf1N1B4_3490 - probable membrane protein YPO2362, at 3,897,816 to 3,898,418 
         
        _3490 
         Position (kb)  
3897 
 
3898 Strain fitness (log2 ratio)  
-3 
 
-2 
 
-1 
 
0 
 
1 
 
2 
 
3 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.203 kb on - strand, within Pf1N1B4_3488 at 3896.203 kb on - strand, within Pf1N1B4_3488 at 3896.203 kb on - strand, within Pf1N1B4_3488 at 3896.238 kb on + strand, within Pf1N1B4_3488 at 3896.238 kb on + strand, within Pf1N1B4_3488 at 3896.239 kb on - strand, within Pf1N1B4_3488 at 3896.239 kb on - strand, within Pf1N1B4_3488 at 3896.268 kb on + strand, within Pf1N1B4_3488 at 3896.269 kb on - strand, within Pf1N1B4_3488 at 3896.269 kb on - strand, within Pf1N1B4_3488 at 3896.295 kb on + strand, within Pf1N1B4_3488 at 3896.296 kb on - strand, within Pf1N1B4_3488 at 3896.548 kb on - strand, within Pf1N1B4_3488 at 3896.548 kb on - strand, within Pf1N1B4_3488 at 3896.548 kb on - strand, within Pf1N1B4_3488 at 3896.553 kb on + strand, within Pf1N1B4_3488 at 3896.553 kb on + strand, within Pf1N1B4_3488 at 3896.553 kb on + strand at 3896.554 kb on - strand, within Pf1N1B4_3488 at 3896.556 kb on + strand, within Pf1N1B4_3488 at 3896.556 kb on + strand, within Pf1N1B4_3488 at 3896.724 kb on + strand, within Pf1N1B4_3488 at 3896.785 kb on + strand at 3896.785 kb on + strand, within Pf1N1B4_3488 at 3896.785 kb on + strand, within Pf1N1B4_3488 at 3896.785 kb on + strand, within Pf1N1B4_3488 at 3896.785 kb on + strand, within Pf1N1B4_3488 at 3896.786 kb on - strand, within Pf1N1B4_3488 at 3896.786 kb on - strand, within Pf1N1B4_3488 at 3896.786 kb on - strand at 3897.001 kb on + strand at 3897.020 kb on + strand at 3897.020 kb on + strand at 3897.020 kb on + strand at 3897.082 kb on + strand at 3897.165 kb on - strand at 3897.165 kb on - strand at 3897.165 kb on - strand at 3897.296 kb on + strand, within Pf1N1B4_3489 at 3897.296 kb on + strand, within Pf1N1B4_3489 at 3897.297 kb on - strand, within Pf1N1B4_3489 at 3897.297 kb on - strand, within Pf1N1B4_3489 at 3897.297 kb on - strand, within Pf1N1B4_3489 at 3897.297 kb on - strand, within Pf1N1B4_3489 at 3897.297 kb on - strand, within Pf1N1B4_3489 at 3897.297 kb on - strand, within Pf1N1B4_3489 at 3897.303 kb on - strand, within Pf1N1B4_3489 at 3897.303 kb on - strand, within Pf1N1B4_3489 at 3897.332 kb on + strand, within Pf1N1B4_3489 at 3897.332 kb on + strand, within Pf1N1B4_3489 at 3897.332 kb on + strand, within Pf1N1B4_3489 at 3897.333 kb on - strand, within Pf1N1B4_3489 at 3897.333 kb on - strand at 3897.333 kb on - strand, within Pf1N1B4_3489 at 3897.362 kb on + strand, within Pf1N1B4_3489 at 3897.758 kb on + strand at 3897.758 kb on + strand at 3897.758 kb on + strand at 3897.758 kb on + strand at 3897.817 kb on + strand at 3897.817 kb on + strand at 3897.818 kb on - strand at 3897.818 kb on - strand at 3897.818 kb on - strand at 3897.818 kb on - strand at 3897.818 kb on - strand at 3897.818 kb on - strand at 3897.825 kb on - strand at 3897.826 kb on + strand at 3897.970 kb on + strand, within Pf1N1B4_3490 at 3897.970 kb on + strand, within Pf1N1B4_3490 at 3897.970 kb on + strand, within Pf1N1B4_3490 at 3897.970 kb on + strand, within Pf1N1B4_3490 at 3897.970 kb on + strand, within Pf1N1B4_3490 at 3897.970 kb on + strand, within Pf1N1B4_3490 at 3897.970 kb on + strand, within Pf1N1B4_3490 at 3897.970 kb on + strand at 3897.971 kb on - strand, within Pf1N1B4_3490 at 3897.971 kb on - strand, within Pf1N1B4_3490 at 3897.971 kb on - strand, within Pf1N1B4_3490 at 3897.971 kb on - strand, within Pf1N1B4_3490 at 3897.979 kb on + strand, within Pf1N1B4_3490 at 3897.979 kb on + strand, within Pf1N1B4_3490 at 3897.979 kb on + strand at 3897.980 kb on - strand, within Pf1N1B4_3490 at 3897.989 kb on - strand, within Pf1N1B4_3490 at 3897.989 kb on - strand, within Pf1N1B4_3490 at 3898.078 kb on + strand, within Pf1N1B4_3490 at 3898.123 kb on + strand, within Pf1N1B4_3490 at 3898.124 kb on - strand, within Pf1N1B4_3490 at 3898.124 kb on - strand, within Pf1N1B4_3490 at 3898.124 kb on - strand, within Pf1N1B4_3490 at 3898.124 kb on - strand, within Pf1N1B4_3490 at 3898.124 kb on - strand, within Pf1N1B4_3490 at 3898.124 kb on - strand at 3898.187 kb on - strand, within Pf1N1B4_3490 at 3898.327 kb on + strand, within Pf1N1B4_3490 at 3898.327 kb on + strand, within Pf1N1B4_3490 at 3898.328 kb on - strand, within Pf1N1B4_3490 at 3898.328 kb on - strand, within Pf1N1B4_3490 at 3898.328 kb on - strand, within Pf1N1B4_3490 at 3898.328 kb on - strand, within Pf1N1B4_3490 at 3898.328 kb on - strand, within Pf1N1B4_3490 at 3898.328 kb on - strand, within Pf1N1B4_3490 at 3898.328 kb on - strand at 3898.404 kb on + strand at 3898.404 kb on + strand at 3898.404 kb on + strand at 3898.404 kb on + strand at 3898.404 kb on + strand at 3898.404 kb on + strand at 3898.404 kb on + strand at 3898.405 kb on - strand at 3898.405 kb on - strand at 3898.405 kb on - strand at 3898.485 kb on + strand at 3898.485 kb on + strand at 3898.485 kb on + strand at 3898.485 kb on + strand at 3898.485 kb on + strand at 3898.485 kb on + strand at 3898.486 kb on - strand at 3898.486 kb on - strand at 3898.486 kb on - strand at 3898.486 kb on - strand at 3898.486 kb on - strand at 3898.486 kb on - strand at 3898.489 kb on + strand at 3898.490 kb on - strand at 3898.490 kb on - strand at 3898.490 kb on - strand at 3898.490 kb on - strand at 3898.501 kb on + strand at 3898.501 kb on + strand at 3898.505 kb on + strand at 3898.506 kb on - strand at 3898.515 kb on + strand at 3898.527 kb on + strand at 3898.527 kb on + strand at 3898.528 kb on - strand at 3898.528 kb on - strand at 3898.528 kb on - strand at 3898.528 kb on - strand at 3898.528 kb on - strand at 3898.547 kb on + strand at 3898.547 kb on + strand at 3898.547 kb on + strand at 3898.548 kb on - strand at 3898.548 kb on - strand at 3898.548 kb on - strand at 3898.548 kb on - strand at 3898.561 kb on + strand at 3898.578 kb on + strand at 3898.579 kb on - strand at 3898.585 kb on + strand  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   Gentamicin 0.001 mg/ml             remove   3,896,202   +    Pf1N1B4_3488   0.30  +0.2   3,896,202   +    Pf1N1B4_3488   0.30  -0.7   3,896,202   +    Pf1N1B4_3488   0.30  +0.6   3,896,202   +    Pf1N1B4_3488   0.30  -1.2   3,896,202   +    Pf1N1B4_3488   0.30  -0.7   3,896,202   +        +1.2   3,896,202   +    Pf1N1B4_3488   0.30  +2.1   3,896,202   +    Pf1N1B4_3488   0.30  -0.1   3,896,202   +    Pf1N1B4_3488   0.30  +1.4   3,896,203   -    Pf1N1B4_3488   0.30  -0.8   3,896,203   -    Pf1N1B4_3488   0.30  -1.0   3,896,203   -    Pf1N1B4_3488   0.30  +1.4   3,896,238   +    Pf1N1B4_3488   0.33  +0.8   3,896,238   +    Pf1N1B4_3488   0.33  +1.0   3,896,239   -    Pf1N1B4_3488   0.33  -0.6   3,896,239   -    Pf1N1B4_3488   0.33  -0.1   3,896,268   +    Pf1N1B4_3488   0.36  -0.6   3,896,269   -    Pf1N1B4_3488   0.36  -1.4   3,896,269   -    Pf1N1B4_3488   0.36  +0.3   3,896,295   +    Pf1N1B4_3488   0.38  +0.1   3,896,296   -    Pf1N1B4_3488   0.38  -0.4   3,896,548   -    Pf1N1B4_3488   0.59  -2.0   3,896,548   -    Pf1N1B4_3488   0.59  -1.0   3,896,548   -    Pf1N1B4_3488   0.59  -0.6   3,896,553   +    Pf1N1B4_3488   0.60  -0.4   3,896,553   +    Pf1N1B4_3488   0.60  +0.4   3,896,553   +        +0.6   3,896,554   -    Pf1N1B4_3488   0.60  -0.1   3,896,556   +    Pf1N1B4_3488   0.60  -2.0   3,896,556   +    Pf1N1B4_3488   0.60  -1.4   3,896,724   +    Pf1N1B4_3488   0.74  +0.0   3,896,785   +        +2.4   3,896,785   +    Pf1N1B4_3488   0.80  +1.4   3,896,785   +    Pf1N1B4_3488   0.80  -1.4   3,896,785   +    Pf1N1B4_3488   0.80  -2.7   3,896,785   +    Pf1N1B4_3488   0.80  -0.1   3,896,786   -    Pf1N1B4_3488   0.80  -0.2   3,896,786   -    Pf1N1B4_3488   0.80  -1.2   3,896,786   -        +0.6   3,897,001   +        +0.5   3,897,020   +        +1.4   3,897,020   +        -0.2   3,897,020   +        +0.6   3,897,082   +        +0.1   3,897,165   -        +0.1   3,897,165   -        -0.7   3,897,165   -        -1.6   3,897,296   +    Pf1N1B4_3489   0.27  +0.6   3,897,296   +    Pf1N1B4_3489   0.27  -1.1   3,897,297   -    Pf1N1B4_3489   0.27  -2.0   3,897,297   -    Pf1N1B4_3489   0.27  +0.6   3,897,297   -    Pf1N1B4_3489   0.27  -0.4   3,897,297   -    Pf1N1B4_3489   0.27  +3.2   3,897,297   -    Pf1N1B4_3489   0.27  -0.1   3,897,297   -    Pf1N1B4_3489   0.27  -0.1   3,897,303   -    Pf1N1B4_3489   0.28  +0.2   3,897,303   -    Pf1N1B4_3489   0.28  +2.4   3,897,332   +    Pf1N1B4_3489   0.34  -1.0   3,897,332   +    Pf1N1B4_3489   0.34  -0.2   3,897,332   +    Pf1N1B4_3489   0.34  -1.0   3,897,333   -    Pf1N1B4_3489   0.34  -0.8   3,897,333   -        +0.6   3,897,333   -    Pf1N1B4_3489   0.34  +1.2   3,897,362   +    Pf1N1B4_3489   0.40  -0.4   3,897,758   +        -0.4   3,897,758   +        -0.1   3,897,758   +        -1.7   3,897,758   +        +1.0   3,897,817   +        -0.8   3,897,817   +        -0.9   3,897,818   -        -1.0   3,897,818   -        -0.8   3,897,818   -        +0.2   3,897,818   -        -0.1   3,897,818   -        -1.0   3,897,818   -        -1.4   3,897,825   -        -1.4   3,897,826   +        -1.7   3,897,970   +    Pf1N1B4_3490   0.26  -1.1   3,897,970   +    Pf1N1B4_3490   0.26  +0.2   3,897,970   +    Pf1N1B4_3490   0.26  -0.5   3,897,970   +    Pf1N1B4_3490   0.26  -1.7   3,897,970   +    Pf1N1B4_3490   0.26  -1.6   3,897,970   +    Pf1N1B4_3490   0.26  +0.8   3,897,970   +    Pf1N1B4_3490   0.26  -0.7   3,897,970   +        +1.9   3,897,971   -    Pf1N1B4_3490   0.26  -0.0   3,897,971   -    Pf1N1B4_3490   0.26  -0.8   3,897,971   -    Pf1N1B4_3490   0.26  -0.5   3,897,971   -    Pf1N1B4_3490   0.26  -2.2   3,897,979   +    Pf1N1B4_3490   0.27  -0.1   3,897,979   +    Pf1N1B4_3490   0.27  -1.1   3,897,979   +        -1.4   3,897,980   -    Pf1N1B4_3490   0.27  -0.4   3,897,989   -    Pf1N1B4_3490   0.29  -1.7   3,897,989   -    Pf1N1B4_3490   0.29  +1.2   3,898,078   +    Pf1N1B4_3490   0.43  +0.4   3,898,123   +    Pf1N1B4_3490   0.51  +0.1   3,898,124   -    Pf1N1B4_3490   0.51  +0.6   3,898,124   -    Pf1N1B4_3490   0.51  -1.0   3,898,124   -    Pf1N1B4_3490   0.51  +0.6   3,898,124   -    Pf1N1B4_3490   0.51  -1.0   3,898,124   -    Pf1N1B4_3490   0.51  +0.6   3,898,124   -        +1.6   3,898,187   -    Pf1N1B4_3490   0.62  -3.2   3,898,327   +    Pf1N1B4_3490   0.85  +0.1   3,898,327   +    Pf1N1B4_3490   0.85  -0.2   3,898,328   -    Pf1N1B4_3490   0.85  -1.6   3,898,328   -    Pf1N1B4_3490   0.85  +0.6   3,898,328   -    Pf1N1B4_3490   0.85  +0.6   3,898,328   -    Pf1N1B4_3490   0.85  +0.0   3,898,328   -    Pf1N1B4_3490   0.85  +0.9   3,898,328   -    Pf1N1B4_3490   0.85  -0.3   3,898,328   -        +0.6   3,898,404   +        +0.3   3,898,404   +        +2.2   3,898,404   +        -1.5   3,898,404   +        -0.3   3,898,404   +        -0.4   3,898,404   +        +0.1   3,898,404   +        -1.0   3,898,405   -        -0.4   3,898,405   -        -0.1   3,898,405   -        -1.4   3,898,485   +        +0.6   3,898,485   +        -0.8   3,898,485   +        -1.0   3,898,485   +        +0.2   3,898,485   +        +1.2   3,898,485   +        +0.6   3,898,486   -        -0.4   3,898,486   -        -1.1   3,898,486   -        -0.4   3,898,486   -        -1.0   3,898,486   -        +0.3   3,898,486   -        -1.7   3,898,489   +        +0.6   3,898,490   -        +1.3   3,898,490   -        +3.2   3,898,490   -        -0.1   3,898,490   -        -0.4   3,898,501   +        +0.6   3,898,501   +        -0.1   3,898,505   +        +1.2   3,898,506   -        -0.2   3,898,515   +        +0.1   3,898,527   +        +0.6   3,898,527   +        +2.2   3,898,528   -        -0.9   3,898,528   -        -0.7   3,898,528   -        -1.4   3,898,528   -        -1.0   3,898,528   -        -2.0   3,898,547   +        -0.4   3,898,547   +        -0.7   3,898,547   +        +1.1   3,898,548   -        +0.0   3,898,548   -        +0.2   3,898,548   -        +1.8   3,898,548   -        -0.4   3,898,561   +        +1.9   3,898,578   +        -1.5   3,898,579   -        -0.7   3,898,585   +        -1.1 
 
Or see this region's nucleotide sequence