Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_327 and Pf1N1B4_328 are separated by 905 nucleotides Pf1N1B4_328 and Pf1N1B4_329 are separated by 531 nucleotides
Pf1N1B4_327 - hypothetical protein, at 385,506 to 386,450
_327
Pf1N1B4_328 - Glucosyltransferase (side chain biosynthesis) (EC 2.4.1.-), at 387,356 to 388,624
_328
Pf1N1B4_329 - Acyltransferase, at 389,156 to 390,208
_329
Position (kb) 387
388
389 Strain fitness (log2 ratio) -2
-1
0
1
2 at 386.938 kb on + strand at 386.939 kb on - strand at 386.940 kb on + strand at 387.303 kb on + strand at 387.304 kb on - strand at 387.305 kb on + strand at 387.453 kb on + strand at 387.722 kb on - strand, within Pf1N1B4_328 at 387.755 kb on + strand, within Pf1N1B4_328 at 387.770 kb on + strand, within Pf1N1B4_328 at 387.967 kb on + strand, within Pf1N1B4_328 at 387.967 kb on + strand, within Pf1N1B4_328 at 387.979 kb on - strand, within Pf1N1B4_328 at 388.032 kb on - strand, within Pf1N1B4_328 at 388.036 kb on - strand, within Pf1N1B4_328 at 388.174 kb on - strand, within Pf1N1B4_328 at 388.268 kb on + strand, within Pf1N1B4_328 at 388.273 kb on - strand, within Pf1N1B4_328 at 388.385 kb on + strand, within Pf1N1B4_328 at 388.461 kb on + strand, within Pf1N1B4_328 at 388.461 kb on + strand, within Pf1N1B4_328 at 388.540 kb on + strand at 388.541 kb on - strand at 388.541 kb on - strand at 388.541 kb on - strand at 388.606 kb on + strand at 388.607 kb on - strand at 388.628 kb on + strand at 388.789 kb on - strand at 388.941 kb on - strand at 388.991 kb on + strand at 389.131 kb on - strand at 389.132 kb on + strand at 389.133 kb on - strand at 389.251 kb on - strand at 389.304 kb on - strand, within Pf1N1B4_329 at 389.617 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 386,938 + +0.1 386,939 - +0.5 386,940 + +0.6 387,303 + +0.5 387,304 - -0.5 387,305 + -1.4 387,453 + -1.9 387,722 - Pf1N1B4_328 0.29 +1.5 387,755 + Pf1N1B4_328 0.31 -0.2 387,770 + Pf1N1B4_328 0.33 +1.0 387,967 + Pf1N1B4_328 0.48 -2.2 387,967 + Pf1N1B4_328 0.48 +0.5 387,979 - Pf1N1B4_328 0.49 +0.8 388,032 - Pf1N1B4_328 0.53 -0.3 388,036 - Pf1N1B4_328 0.54 +2.2 388,174 - Pf1N1B4_328 0.64 +0.9 388,268 + Pf1N1B4_328 0.72 -0.0 388,273 - Pf1N1B4_328 0.72 +2.1 388,385 + Pf1N1B4_328 0.81 -0.1 388,461 + Pf1N1B4_328 0.87 +1.7 388,461 + Pf1N1B4_328 0.87 +0.7 388,540 + +0.8 388,541 - +0.5 388,541 - +0.3 388,541 - -0.0 388,606 + -2.3 388,607 - +0.7 388,628 + +0.5 388,789 - -1.1 388,941 - -0.1 388,991 + +1.1 389,131 - +0.2 389,132 + +0.6 389,133 - -0.1 389,251 - -0.2 389,304 - Pf1N1B4_329 0.14 -0.5 389,617 - -1.1
Or see this region's nucleotide sequence