Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3250 and Pf1N1B4_3251 are separated by 22 nucleotides Pf1N1B4_3251 and Pf1N1B4_3252 are separated by 2 nucleotides Pf1N1B4_3252 and Pf1N1B4_3253 are separated by 154 nucleotides
Pf1N1B4_3250 - NADH:flavin oxidoreductases, Old Yellow Enzyme family, at 3,640,171 to 3,641,220
_3250
Pf1N1B4_3251 - Putative sugar transport protein, at 3,641,243 to 3,642,409
_3251
Pf1N1B4_3252 - Transcriptional regulator, ArsR family, at 3,642,412 to 3,642,732
_3252
Pf1N1B4_3253 - Acyl carrier protein phosphodiesterase (EC 3.1.4.14), at 3,642,887 to 3,643,462
_3253
Position (kb)
3641
3642
3643 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3640.294 kb on + strand, within Pf1N1B4_3250 at 3640.376 kb on + strand, within Pf1N1B4_3250 at 3640.376 kb on + strand, within Pf1N1B4_3250 at 3640.376 kb on + strand, within Pf1N1B4_3250 at 3640.376 kb on + strand, within Pf1N1B4_3250 at 3640.377 kb on - strand, within Pf1N1B4_3250 at 3640.377 kb on - strand, within Pf1N1B4_3250 at 3640.377 kb on - strand, within Pf1N1B4_3250 at 3640.484 kb on + strand, within Pf1N1B4_3250 at 3640.484 kb on + strand, within Pf1N1B4_3250 at 3640.484 kb on + strand, within Pf1N1B4_3250 at 3640.484 kb on + strand, within Pf1N1B4_3250 at 3640.485 kb on - strand, within Pf1N1B4_3250 at 3640.485 kb on - strand, within Pf1N1B4_3250 at 3640.485 kb on - strand, within Pf1N1B4_3250 at 3640.485 kb on - strand, within Pf1N1B4_3250 at 3640.485 kb on - strand, within Pf1N1B4_3250 at 3640.485 kb on - strand, within Pf1N1B4_3250 at 3640.485 kb on - strand, within Pf1N1B4_3250 at 3640.485 kb on - strand, within Pf1N1B4_3250 at 3640.485 kb on - strand, within Pf1N1B4_3250 at 3640.485 kb on - strand, within Pf1N1B4_3250 at 3640.637 kb on + strand, within Pf1N1B4_3250 at 3640.637 kb on + strand, within Pf1N1B4_3250 at 3640.637 kb on + strand, within Pf1N1B4_3250 at 3640.637 kb on + strand, within Pf1N1B4_3250 at 3640.637 kb on + strand, within Pf1N1B4_3250 at 3640.637 kb on + strand, within Pf1N1B4_3250 at 3640.638 kb on - strand, within Pf1N1B4_3250 at 3640.638 kb on - strand, within Pf1N1B4_3250 at 3640.638 kb on - strand, within Pf1N1B4_3250 at 3640.689 kb on - strand, within Pf1N1B4_3250 at 3640.892 kb on + strand, within Pf1N1B4_3250 at 3640.893 kb on - strand, within Pf1N1B4_3250 at 3641.090 kb on - strand, within Pf1N1B4_3250 at 3641.090 kb on - strand, within Pf1N1B4_3250 at 3641.090 kb on - strand, within Pf1N1B4_3250 at 3641.090 kb on - strand, within Pf1N1B4_3250 at 3641.090 kb on - strand, within Pf1N1B4_3250 at 3641.090 kb on - strand, within Pf1N1B4_3250 at 3641.090 kb on - strand, within Pf1N1B4_3250 at 3641.093 kb on + strand, within Pf1N1B4_3250 at 3641.093 kb on + strand, within Pf1N1B4_3250 at 3641.093 kb on + strand, within Pf1N1B4_3250 at 3641.094 kb on - strand, within Pf1N1B4_3250 at 3641.094 kb on - strand, within Pf1N1B4_3250 at 3641.094 kb on - strand, within Pf1N1B4_3250 at 3641.096 kb on + strand, within Pf1N1B4_3250 at 3641.097 kb on - strand, within Pf1N1B4_3250 at 3641.097 kb on - strand, within Pf1N1B4_3250 at 3641.211 kb on + strand at 3641.223 kb on - strand at 3641.223 kb on - strand at 3641.223 kb on - strand at 3641.225 kb on + strand at 3641.225 kb on + strand at 3641.226 kb on - strand at 3641.226 kb on - strand at 3641.226 kb on - strand at 3641.248 kb on + strand at 3641.248 kb on + strand at 3641.248 kb on + strand at 3641.248 kb on + strand at 3641.249 kb on - strand at 3641.249 kb on - strand at 3641.249 kb on - strand at 3641.252 kb on - strand at 3641.252 kb on - strand at 3641.290 kb on + strand at 3641.290 kb on + strand at 3641.291 kb on - strand at 3641.291 kb on - strand at 3641.291 kb on - strand at 3641.291 kb on - strand at 3641.291 kb on - strand at 3641.748 kb on + strand, within Pf1N1B4_3251 at 3641.748 kb on + strand, within Pf1N1B4_3251 at 3641.748 kb on + strand, within Pf1N1B4_3251 at 3641.748 kb on + strand, within Pf1N1B4_3251 at 3641.749 kb on - strand, within Pf1N1B4_3251 at 3641.749 kb on - strand, within Pf1N1B4_3251 at 3641.749 kb on - strand, within Pf1N1B4_3251 at 3641.749 kb on - strand, within Pf1N1B4_3251 at 3641.749 kb on - strand, within Pf1N1B4_3251 at 3641.749 kb on - strand, within Pf1N1B4_3251 at 3641.749 kb on - strand, within Pf1N1B4_3251 at 3641.749 kb on - strand, within Pf1N1B4_3251 at 3641.749 kb on - strand, within Pf1N1B4_3251 at 3641.749 kb on - strand, within Pf1N1B4_3251 at 3641.749 kb on - strand, within Pf1N1B4_3251 at 3641.749 kb on - strand, within Pf1N1B4_3251 at 3641.749 kb on - strand, within Pf1N1B4_3251 at 3641.749 kb on - strand, within Pf1N1B4_3251 at 3641.749 kb on - strand, within Pf1N1B4_3251 at 3641.749 kb on - strand, within Pf1N1B4_3251 at 3641.749 kb on - strand, within Pf1N1B4_3251 at 3641.749 kb on - strand, within Pf1N1B4_3251 at 3641.780 kb on + strand, within Pf1N1B4_3251 at 3642.138 kb on + strand, within Pf1N1B4_3251 at 3642.138 kb on + strand, within Pf1N1B4_3251 at 3642.138 kb on + strand, within Pf1N1B4_3251 at 3642.138 kb on + strand, within Pf1N1B4_3251 at 3642.139 kb on - strand, within Pf1N1B4_3251 at 3642.139 kb on - strand, within Pf1N1B4_3251 at 3642.139 kb on - strand, within Pf1N1B4_3251 at 3642.139 kb on - strand, within Pf1N1B4_3251 at 3642.195 kb on - strand, within Pf1N1B4_3251 at 3642.615 kb on - strand, within Pf1N1B4_3252 at 3642.615 kb on - strand, within Pf1N1B4_3252 at 3642.686 kb on - strand, within Pf1N1B4_3252 at 3642.707 kb on - strand at 3642.707 kb on - strand at 3642.709 kb on + strand at 3642.710 kb on - strand at 3642.760 kb on + strand at 3642.808 kb on + strand at 3642.851 kb on - strand at 3642.852 kb on + strand at 3642.876 kb on - strand at 3642.876 kb on - strand at 3642.876 kb on - strand at 3642.876 kb on - strand at 3642.876 kb on - strand at 3642.893 kb on + strand at 3642.893 kb on + strand at 3642.893 kb on + strand at 3642.893 kb on + strand at 3642.893 kb on + strand at 3642.893 kb on + strand at 3642.893 kb on + strand at 3642.893 kb on + strand at 3642.894 kb on - strand at 3642.894 kb on - strand at 3642.894 kb on - strand at 3642.894 kb on - strand at 3642.894 kb on - strand at 3642.894 kb on - strand at 3642.894 kb on - strand at 3642.951 kb on - strand, within Pf1N1B4_3253 at 3643.160 kb on + strand at 3643.161 kb on - strand, within Pf1N1B4_3253 at 3643.161 kb on - strand, within Pf1N1B4_3253 at 3643.388 kb on + strand, within Pf1N1B4_3253 at 3643.388 kb on + strand, within Pf1N1B4_3253 at 3643.388 kb on + strand, within Pf1N1B4_3253 at 3643.388 kb on + strand, within Pf1N1B4_3253 at 3643.388 kb on + strand, within Pf1N1B4_3253 at 3643.389 kb on - strand, within Pf1N1B4_3253
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 3,640,294 + Pf1N1B4_3250 0.12 +0.1 3,640,376 + Pf1N1B4_3250 0.20 +1.9 3,640,376 + Pf1N1B4_3250 0.20 +1.5 3,640,376 + Pf1N1B4_3250 0.20 -0.6 3,640,376 + Pf1N1B4_3250 0.20 -0.3 3,640,377 - Pf1N1B4_3250 0.20 +0.7 3,640,377 - Pf1N1B4_3250 0.20 -1.1 3,640,377 - Pf1N1B4_3250 0.20 +1.2 3,640,484 + Pf1N1B4_3250 0.30 -1.3 3,640,484 + Pf1N1B4_3250 0.30 -0.9 3,640,484 + Pf1N1B4_3250 0.30 +0.5 3,640,484 + Pf1N1B4_3250 0.30 +0.4 3,640,485 - Pf1N1B4_3250 0.30 +0.7 3,640,485 - Pf1N1B4_3250 0.30 -0.0 3,640,485 - Pf1N1B4_3250 0.30 -1.5 3,640,485 - Pf1N1B4_3250 0.30 -2.2 3,640,485 - Pf1N1B4_3250 0.30 -0.3 3,640,485 - Pf1N1B4_3250 0.30 +0.1 3,640,485 - Pf1N1B4_3250 0.30 -0.0 3,640,485 - Pf1N1B4_3250 0.30 -0.5 3,640,485 - Pf1N1B4_3250 0.30 +0.2 3,640,485 - Pf1N1B4_3250 0.30 -0.9 3,640,637 + Pf1N1B4_3250 0.44 +0.7 3,640,637 + Pf1N1B4_3250 0.44 -0.5 3,640,637 + Pf1N1B4_3250 0.44 +1.0 3,640,637 + Pf1N1B4_3250 0.44 +1.0 3,640,637 + Pf1N1B4_3250 0.44 -0.5 3,640,637 + Pf1N1B4_3250 0.44 +0.2 3,640,638 - Pf1N1B4_3250 0.44 -1.3 3,640,638 - Pf1N1B4_3250 0.44 +0.1 3,640,638 - Pf1N1B4_3250 0.44 -0.6 3,640,689 - Pf1N1B4_3250 0.49 -0.9 3,640,892 + Pf1N1B4_3250 0.69 +0.0 3,640,893 - Pf1N1B4_3250 0.69 +0.1 3,641,090 - Pf1N1B4_3250 0.88 +0.7 3,641,090 - Pf1N1B4_3250 0.88 +1.2 3,641,090 - Pf1N1B4_3250 0.88 +0.3 3,641,090 - Pf1N1B4_3250 0.88 -0.7 3,641,090 - Pf1N1B4_3250 0.88 -0.9 3,641,090 - Pf1N1B4_3250 0.88 -0.1 3,641,090 - Pf1N1B4_3250 0.88 +1.4 3,641,093 + Pf1N1B4_3250 0.88 +2.5 3,641,093 + Pf1N1B4_3250 0.88 +0.5 3,641,093 + Pf1N1B4_3250 0.88 +1.8 3,641,094 - Pf1N1B4_3250 0.88 +0.6 3,641,094 - Pf1N1B4_3250 0.88 +0.7 3,641,094 - Pf1N1B4_3250 0.88 -0.1 3,641,096 + Pf1N1B4_3250 0.88 +0.1 3,641,097 - Pf1N1B4_3250 0.88 -0.9 3,641,097 - Pf1N1B4_3250 0.88 +0.2 3,641,211 + -0.6 3,641,223 - +1.0 3,641,223 - +0.4 3,641,223 - +1.3 3,641,225 + +0.7 3,641,225 + -0.0 3,641,226 - -0.0 3,641,226 - +1.3 3,641,226 - -0.3 3,641,248 + -0.9 3,641,248 + +0.7 3,641,248 + +0.1 3,641,248 + -0.1 3,641,249 - -0.8 3,641,249 - -0.5 3,641,249 - -0.4 3,641,252 - +0.7 3,641,252 - -1.1 3,641,290 + +0.2 3,641,290 + -0.3 3,641,291 - +1.1 3,641,291 - -0.3 3,641,291 - -1.0 3,641,291 - -2.1 3,641,291 - -0.3 3,641,748 + Pf1N1B4_3251 0.43 +0.7 3,641,748 + Pf1N1B4_3251 0.43 -0.6 3,641,748 + Pf1N1B4_3251 0.43 +2.3 3,641,748 + Pf1N1B4_3251 0.43 +0.2 3,641,749 - Pf1N1B4_3251 0.43 +0.1 3,641,749 - Pf1N1B4_3251 0.43 -1.6 3,641,749 - Pf1N1B4_3251 0.43 -1.9 3,641,749 - Pf1N1B4_3251 0.43 -0.8 3,641,749 - Pf1N1B4_3251 0.43 -0.3 3,641,749 - Pf1N1B4_3251 0.43 -1.6 3,641,749 - Pf1N1B4_3251 0.43 +1.0 3,641,749 - Pf1N1B4_3251 0.43 -2.3 3,641,749 - Pf1N1B4_3251 0.43 +0.7 3,641,749 - Pf1N1B4_3251 0.43 +0.1 3,641,749 - Pf1N1B4_3251 0.43 +0.4 3,641,749 - Pf1N1B4_3251 0.43 -0.7 3,641,749 - Pf1N1B4_3251 0.43 -0.9 3,641,749 - Pf1N1B4_3251 0.43 -1.9 3,641,749 - Pf1N1B4_3251 0.43 -0.8 3,641,749 - Pf1N1B4_3251 0.43 -1.0 3,641,749 - Pf1N1B4_3251 0.43 +1.3 3,641,749 - Pf1N1B4_3251 0.43 -1.3 3,641,780 + Pf1N1B4_3251 0.46 -0.1 3,642,138 + Pf1N1B4_3251 0.77 -2.1 3,642,138 + Pf1N1B4_3251 0.77 -1.1 3,642,138 + Pf1N1B4_3251 0.77 -1.5 3,642,138 + Pf1N1B4_3251 0.77 -2.1 3,642,139 - Pf1N1B4_3251 0.77 -1.3 3,642,139 - Pf1N1B4_3251 0.77 +0.1 3,642,139 - Pf1N1B4_3251 0.77 -0.2 3,642,139 - Pf1N1B4_3251 0.77 -1.6 3,642,195 - Pf1N1B4_3251 0.82 +0.1 3,642,615 - Pf1N1B4_3252 0.63 +0.7 3,642,615 - Pf1N1B4_3252 0.63 -1.7 3,642,686 - Pf1N1B4_3252 0.85 +0.4 3,642,707 - -3.0 3,642,707 - -1.3 3,642,709 + +0.0 3,642,710 - +1.1 3,642,760 + -1.9 3,642,808 + -0.3 3,642,851 - -0.4 3,642,852 + -0.9 3,642,876 - +1.7 3,642,876 - -2.1 3,642,876 - -0.0 3,642,876 - +1.1 3,642,876 - -0.4 3,642,893 + +0.5 3,642,893 + +0.2 3,642,893 + -0.6 3,642,893 + -0.3 3,642,893 + +1.1 3,642,893 + -0.3 3,642,893 + -0.7 3,642,893 + -0.3 3,642,894 - -0.4 3,642,894 - +2.5 3,642,894 - -1.0 3,642,894 - +0.1 3,642,894 - -0.1 3,642,894 - +0.7 3,642,894 - -0.0 3,642,951 - Pf1N1B4_3253 0.11 +1.7 3,643,160 + -1.3 3,643,161 - Pf1N1B4_3253 0.48 +0.4 3,643,161 - Pf1N1B4_3253 0.48 -1.3 3,643,388 + Pf1N1B4_3253 0.87 +0.1 3,643,388 + Pf1N1B4_3253 0.87 -1.3 3,643,388 + Pf1N1B4_3253 0.87 +0.4 3,643,388 + Pf1N1B4_3253 0.87 -0.1 3,643,388 + Pf1N1B4_3253 0.87 -0.0 3,643,389 - Pf1N1B4_3253 0.87 +0.1
Or see this region's nucleotide sequence