Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3248 and Pf1N1B4_3249 are separated by 161 nucleotides Pf1N1B4_3249 and Pf1N1B4_3250 are separated by 149 nucleotides
Pf1N1B4_3248 - Transcription repressor of multidrug efflux pump acrAB operon, TetR (AcrR) family, at 3,638,343 to 3,638,975
_3248
Pf1N1B4_3249 - Arginine/ornithine antiporter ArcD, at 3,639,137 to 3,640,021
_3249
Pf1N1B4_3250 - NADH:flavin oxidoreductases, Old Yellow Enzyme family, at 3,640,171 to 3,641,220
_3250
Position (kb)
3639
3640
3641 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3638.154 kb on - strand at 3638.210 kb on - strand at 3638.249 kb on + strand at 3638.249 kb on + strand at 3638.299 kb on + strand at 3638.337 kb on - strand at 3638.415 kb on + strand, within Pf1N1B4_3248 at 3638.415 kb on + strand, within Pf1N1B4_3248 at 3638.416 kb on - strand, within Pf1N1B4_3248 at 3638.487 kb on + strand, within Pf1N1B4_3248 at 3638.487 kb on + strand, within Pf1N1B4_3248 at 3638.488 kb on - strand, within Pf1N1B4_3248 at 3638.504 kb on + strand, within Pf1N1B4_3248 at 3638.687 kb on + strand, within Pf1N1B4_3248 at 3638.687 kb on + strand, within Pf1N1B4_3248 at 3638.687 kb on + strand, within Pf1N1B4_3248 at 3638.688 kb on - strand, within Pf1N1B4_3248 at 3638.688 kb on - strand, within Pf1N1B4_3248 at 3638.850 kb on + strand, within Pf1N1B4_3248 at 3638.850 kb on + strand, within Pf1N1B4_3248 at 3638.851 kb on - strand, within Pf1N1B4_3248 at 3638.851 kb on - strand, within Pf1N1B4_3248 at 3638.942 kb on + strand at 3638.987 kb on + strand at 3638.988 kb on - strand at 3639.050 kb on + strand at 3639.050 kb on + strand at 3639.050 kb on + strand at 3639.051 kb on - strand at 3639.064 kb on + strand at 3639.064 kb on + strand at 3639.064 kb on + strand at 3639.064 kb on + strand at 3639.064 kb on + strand at 3639.064 kb on + strand at 3639.064 kb on + strand at 3639.064 kb on + strand at 3639.065 kb on - strand at 3639.065 kb on - strand at 3639.065 kb on - strand at 3639.065 kb on - strand at 3639.065 kb on - strand at 3639.065 kb on - strand at 3639.066 kb on + strand at 3639.066 kb on + strand at 3639.066 kb on + strand at 3639.066 kb on + strand at 3639.066 kb on + strand at 3639.068 kb on + strand at 3639.068 kb on + strand at 3639.068 kb on + strand at 3639.068 kb on + strand at 3639.068 kb on + strand at 3639.068 kb on + strand at 3639.068 kb on + strand at 3639.068 kb on + strand at 3639.068 kb on + strand at 3639.068 kb on + strand at 3639.068 kb on + strand at 3639.068 kb on + strand at 3639.068 kb on + strand at 3639.068 kb on + strand at 3639.068 kb on + strand at 3639.068 kb on + strand at 3639.068 kb on + strand at 3639.068 kb on + strand at 3639.069 kb on - strand at 3639.069 kb on - strand at 3639.069 kb on - strand at 3639.069 kb on - strand at 3639.069 kb on - strand at 3639.072 kb on + strand at 3639.072 kb on + strand at 3639.072 kb on + strand at 3639.072 kb on + strand at 3639.072 kb on + strand at 3639.072 kb on + strand at 3639.072 kb on + strand at 3639.072 kb on + strand at 3639.072 kb on + strand at 3639.072 kb on + strand at 3639.072 kb on + strand at 3639.073 kb on - strand at 3639.073 kb on - strand at 3639.073 kb on - strand at 3639.073 kb on - strand at 3639.073 kb on - strand at 3639.073 kb on - strand at 3639.089 kb on + strand at 3639.089 kb on + strand at 3639.089 kb on + strand at 3639.089 kb on + strand at 3639.090 kb on - strand at 3639.090 kb on - strand at 3639.102 kb on - strand at 3639.105 kb on + strand at 3639.105 kb on + strand at 3639.105 kb on + strand at 3639.105 kb on + strand at 3639.105 kb on + strand at 3639.106 kb on - strand at 3639.245 kb on + strand, within Pf1N1B4_3249 at 3639.245 kb on + strand, within Pf1N1B4_3249 at 3639.245 kb on + strand, within Pf1N1B4_3249 at 3639.246 kb on - strand, within Pf1N1B4_3249 at 3639.290 kb on + strand, within Pf1N1B4_3249 at 3639.290 kb on + strand, within Pf1N1B4_3249 at 3639.290 kb on + strand at 3639.382 kb on - strand, within Pf1N1B4_3249 at 3639.385 kb on - strand, within Pf1N1B4_3249 at 3639.385 kb on - strand, within Pf1N1B4_3249 at 3639.437 kb on + strand, within Pf1N1B4_3249 at 3639.438 kb on - strand, within Pf1N1B4_3249 at 3639.483 kb on + strand, within Pf1N1B4_3249 at 3639.484 kb on - strand, within Pf1N1B4_3249 at 3639.484 kb on - strand, within Pf1N1B4_3249 at 3639.653 kb on + strand, within Pf1N1B4_3249 at 3639.654 kb on - strand, within Pf1N1B4_3249 at 3639.654 kb on - strand, within Pf1N1B4_3249 at 3639.887 kb on + strand, within Pf1N1B4_3249 at 3639.887 kb on + strand, within Pf1N1B4_3249 at 3639.915 kb on - strand, within Pf1N1B4_3249 at 3639.915 kb on - strand, within Pf1N1B4_3249 at 3639.920 kb on + strand, within Pf1N1B4_3249 at 3639.921 kb on - strand, within Pf1N1B4_3249 at 3640.023 kb on + strand at 3640.023 kb on + strand at 3640.063 kb on - strand at 3640.071 kb on + strand at 3640.071 kb on + strand at 3640.072 kb on - strand at 3640.072 kb on - strand at 3640.072 kb on - strand at 3640.106 kb on + strand at 3640.106 kb on + strand at 3640.106 kb on + strand at 3640.106 kb on + strand at 3640.106 kb on + strand at 3640.106 kb on + strand at 3640.106 kb on + strand at 3640.106 kb on + strand at 3640.106 kb on + strand at 3640.106 kb on + strand at 3640.107 kb on - strand at 3640.107 kb on - strand at 3640.107 kb on - strand at 3640.108 kb on + strand at 3640.109 kb on - strand at 3640.173 kb on - strand at 3640.194 kb on - strand at 3640.215 kb on - strand at 3640.215 kb on - strand at 3640.215 kb on - strand at 3640.215 kb on - strand at 3640.294 kb on + strand, within Pf1N1B4_3250 at 3640.376 kb on + strand, within Pf1N1B4_3250 at 3640.376 kb on + strand, within Pf1N1B4_3250 at 3640.376 kb on + strand, within Pf1N1B4_3250 at 3640.376 kb on + strand, within Pf1N1B4_3250 at 3640.377 kb on - strand, within Pf1N1B4_3250 at 3640.377 kb on - strand, within Pf1N1B4_3250 at 3640.377 kb on - strand, within Pf1N1B4_3250 at 3640.484 kb on + strand, within Pf1N1B4_3250 at 3640.484 kb on + strand, within Pf1N1B4_3250 at 3640.484 kb on + strand, within Pf1N1B4_3250 at 3640.484 kb on + strand, within Pf1N1B4_3250 at 3640.485 kb on - strand, within Pf1N1B4_3250 at 3640.485 kb on - strand, within Pf1N1B4_3250 at 3640.485 kb on - strand, within Pf1N1B4_3250 at 3640.485 kb on - strand, within Pf1N1B4_3250 at 3640.485 kb on - strand, within Pf1N1B4_3250 at 3640.485 kb on - strand, within Pf1N1B4_3250 at 3640.485 kb on - strand, within Pf1N1B4_3250 at 3640.485 kb on - strand, within Pf1N1B4_3250 at 3640.485 kb on - strand, within Pf1N1B4_3250 at 3640.485 kb on - strand, within Pf1N1B4_3250 at 3640.637 kb on + strand, within Pf1N1B4_3250 at 3640.637 kb on + strand, within Pf1N1B4_3250 at 3640.637 kb on + strand, within Pf1N1B4_3250 at 3640.637 kb on + strand, within Pf1N1B4_3250 at 3640.637 kb on + strand, within Pf1N1B4_3250 at 3640.637 kb on + strand, within Pf1N1B4_3250 at 3640.638 kb on - strand, within Pf1N1B4_3250 at 3640.638 kb on - strand, within Pf1N1B4_3250 at 3640.638 kb on - strand, within Pf1N1B4_3250 at 3640.689 kb on - strand, within Pf1N1B4_3250 at 3640.892 kb on + strand, within Pf1N1B4_3250 at 3640.893 kb on - strand, within Pf1N1B4_3250
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 3,638,154 - +0.1 3,638,210 - +0.3 3,638,249 + -0.2 3,638,249 + -0.9 3,638,299 + +2.0 3,638,337 - -1.1 3,638,415 + Pf1N1B4_3248 0.11 +1.0 3,638,415 + Pf1N1B4_3248 0.11 -0.9 3,638,416 - Pf1N1B4_3248 0.12 +2.4 3,638,487 + Pf1N1B4_3248 0.23 +0.7 3,638,487 + Pf1N1B4_3248 0.23 -0.6 3,638,488 - Pf1N1B4_3248 0.23 +0.3 3,638,504 + Pf1N1B4_3248 0.25 +1.9 3,638,687 + Pf1N1B4_3248 0.54 -1.6 3,638,687 + Pf1N1B4_3248 0.54 +0.3 3,638,687 + Pf1N1B4_3248 0.54 +0.1 3,638,688 - Pf1N1B4_3248 0.55 -0.3 3,638,688 - Pf1N1B4_3248 0.55 -0.3 3,638,850 + Pf1N1B4_3248 0.80 +0.1 3,638,850 + Pf1N1B4_3248 0.80 -0.3 3,638,851 - Pf1N1B4_3248 0.80 -0.3 3,638,851 - Pf1N1B4_3248 0.80 -0.0 3,638,942 + -0.7 3,638,987 + -1.9 3,638,988 - -0.9 3,639,050 + -0.7 3,639,050 + +1.2 3,639,050 + -1.1 3,639,051 - +0.3 3,639,064 + -0.7 3,639,064 + +1.7 3,639,064 + +0.7 3,639,064 + +0.7 3,639,064 + -0.5 3,639,064 + -1.5 3,639,064 + -0.7 3,639,064 + -0.0 3,639,065 - -0.2 3,639,065 - +2.0 3,639,065 - -0.3 3,639,065 - -0.6 3,639,065 - -0.4 3,639,065 - +0.2 3,639,066 + +0.8 3,639,066 + -0.9 3,639,066 + -0.6 3,639,066 + -1.9 3,639,066 + -1.3 3,639,068 + -2.3 3,639,068 + +0.4 3,639,068 + -0.0 3,639,068 + +0.3 3,639,068 + +0.7 3,639,068 + -1.0 3,639,068 + +0.7 3,639,068 + +0.4 3,639,068 + -0.6 3,639,068 + -0.9 3,639,068 + -2.5 3,639,068 + -1.5 3,639,068 + +0.7 3,639,068 + +0.7 3,639,068 + +0.2 3,639,068 + -0.9 3,639,068 + +0.3 3,639,068 + -0.9 3,639,069 - -0.9 3,639,069 - -1.6 3,639,069 - +0.9 3,639,069 - +0.7 3,639,069 - -1.0 3,639,072 + +2.3 3,639,072 + -0.9 3,639,072 + +0.7 3,639,072 + +0.5 3,639,072 + -3.0 3,639,072 + +2.3 3,639,072 + +1.0 3,639,072 + -0.3 3,639,072 + +1.3 3,639,072 + -0.1 3,639,072 + -0.6 3,639,073 - +0.7 3,639,073 - +0.7 3,639,073 - -0.5 3,639,073 - +0.7 3,639,073 - +1.0 3,639,073 - -0.0 3,639,089 + -0.1 3,639,089 + -0.1 3,639,089 + -1.3 3,639,089 + -0.6 3,639,090 - -0.9 3,639,090 - +1.5 3,639,102 - +1.7 3,639,105 + +0.2 3,639,105 + +1.7 3,639,105 + +0.7 3,639,105 + +0.3 3,639,105 + +0.4 3,639,106 - +0.1 3,639,245 + Pf1N1B4_3249 0.12 +0.7 3,639,245 + Pf1N1B4_3249 0.12 -0.6 3,639,245 + Pf1N1B4_3249 0.12 -1.4 3,639,246 - Pf1N1B4_3249 0.12 -0.3 3,639,290 + Pf1N1B4_3249 0.17 -1.1 3,639,290 + Pf1N1B4_3249 0.17 +0.5 3,639,290 + -1.3 3,639,382 - Pf1N1B4_3249 0.28 +1.5 3,639,385 - Pf1N1B4_3249 0.28 -0.3 3,639,385 - Pf1N1B4_3249 0.28 -1.6 3,639,437 + Pf1N1B4_3249 0.34 +0.7 3,639,438 - Pf1N1B4_3249 0.34 -0.3 3,639,483 + Pf1N1B4_3249 0.39 -1.9 3,639,484 - Pf1N1B4_3249 0.39 +1.0 3,639,484 - Pf1N1B4_3249 0.39 -0.5 3,639,653 + Pf1N1B4_3249 0.58 +0.2 3,639,654 - Pf1N1B4_3249 0.58 +0.7 3,639,654 - Pf1N1B4_3249 0.58 +1.4 3,639,887 + Pf1N1B4_3249 0.85 +0.2 3,639,887 + Pf1N1B4_3249 0.85 -0.3 3,639,915 - Pf1N1B4_3249 0.88 -1.0 3,639,915 - Pf1N1B4_3249 0.88 +0.5 3,639,920 + Pf1N1B4_3249 0.88 -2.1 3,639,921 - Pf1N1B4_3249 0.89 +0.4 3,640,023 + -0.5 3,640,023 + -0.3 3,640,063 - +1.1 3,640,071 + -1.1 3,640,071 + +1.3 3,640,072 - -1.7 3,640,072 - +0.3 3,640,072 - -0.6 3,640,106 + -0.0 3,640,106 + -0.3 3,640,106 + +2.7 3,640,106 + +0.5 3,640,106 + -1.4 3,640,106 + +0.9 3,640,106 + -1.9 3,640,106 + -2.3 3,640,106 + -1.7 3,640,106 + +0.7 3,640,107 - +0.7 3,640,107 - -1.6 3,640,107 - -0.9 3,640,108 + -0.6 3,640,109 - +0.4 3,640,173 - -0.3 3,640,194 - +0.8 3,640,215 - +3.3 3,640,215 - -0.1 3,640,215 - +0.2 3,640,215 - +1.0 3,640,294 + Pf1N1B4_3250 0.12 +0.1 3,640,376 + Pf1N1B4_3250 0.20 +1.9 3,640,376 + Pf1N1B4_3250 0.20 +1.5 3,640,376 + Pf1N1B4_3250 0.20 -0.6 3,640,376 + Pf1N1B4_3250 0.20 -0.3 3,640,377 - Pf1N1B4_3250 0.20 +0.7 3,640,377 - Pf1N1B4_3250 0.20 -1.1 3,640,377 - Pf1N1B4_3250 0.20 +1.2 3,640,484 + Pf1N1B4_3250 0.30 -1.3 3,640,484 + Pf1N1B4_3250 0.30 -0.9 3,640,484 + Pf1N1B4_3250 0.30 +0.5 3,640,484 + Pf1N1B4_3250 0.30 +0.4 3,640,485 - Pf1N1B4_3250 0.30 +0.7 3,640,485 - Pf1N1B4_3250 0.30 -0.0 3,640,485 - Pf1N1B4_3250 0.30 -1.5 3,640,485 - Pf1N1B4_3250 0.30 -2.2 3,640,485 - Pf1N1B4_3250 0.30 -0.3 3,640,485 - Pf1N1B4_3250 0.30 +0.1 3,640,485 - Pf1N1B4_3250 0.30 -0.0 3,640,485 - Pf1N1B4_3250 0.30 -0.5 3,640,485 - Pf1N1B4_3250 0.30 +0.2 3,640,485 - Pf1N1B4_3250 0.30 -0.9 3,640,637 + Pf1N1B4_3250 0.44 +0.7 3,640,637 + Pf1N1B4_3250 0.44 -0.5 3,640,637 + Pf1N1B4_3250 0.44 +1.0 3,640,637 + Pf1N1B4_3250 0.44 +1.0 3,640,637 + Pf1N1B4_3250 0.44 -0.5 3,640,637 + Pf1N1B4_3250 0.44 +0.2 3,640,638 - Pf1N1B4_3250 0.44 -1.3 3,640,638 - Pf1N1B4_3250 0.44 +0.1 3,640,638 - Pf1N1B4_3250 0.44 -0.6 3,640,689 - Pf1N1B4_3250 0.49 -0.9 3,640,892 + Pf1N1B4_3250 0.69 +0.0 3,640,893 - Pf1N1B4_3250 0.69 +0.1
Or see this region's nucleotide sequence