Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3212 and Pf1N1B4_3213 overlap by 7 nucleotides Pf1N1B4_3213 and Pf1N1B4_3214 are separated by 369 nucleotides Pf1N1B4_3214 and Pf1N1B4_3215 are separated by 0 nucleotides Pf1N1B4_3215 and Pf1N1B4_3216 overlap by 4 nucleotides
Pf1N1B4_3212 - hypothetical protein, at 3,594,481 to 3,595,173
_3212
Pf1N1B4_3213 - hypothetical protein, at 3,595,167 to 3,595,376
_3213
Pf1N1B4_3214 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 3,595,746 to 3,596,447
_3214
Pf1N1B4_3215 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 3,596,448 to 3,597,215
_3215
Pf1N1B4_3216 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 3,597,212 to 3,598,468
_3216
Position (kb)
3595
3596
3597 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3594.785 kb on + strand, within Pf1N1B4_3212 at 3594.786 kb on - strand, within Pf1N1B4_3212 at 3594.870 kb on - strand, within Pf1N1B4_3212 at 3594.870 kb on - strand, within Pf1N1B4_3212 at 3595.148 kb on - strand at 3595.227 kb on - strand, within Pf1N1B4_3213 at 3595.433 kb on - strand at 3595.437 kb on - strand at 3595.504 kb on - strand at 3595.596 kb on - strand at 3595.650 kb on + strand at 3595.650 kb on + strand at 3595.650 kb on + strand at 3595.650 kb on + strand at 3595.662 kb on - strand at 3595.700 kb on + strand at 3595.759 kb on + strand at 3595.837 kb on + strand, within Pf1N1B4_3214 at 3595.837 kb on + strand at 3595.837 kb on + strand, within Pf1N1B4_3214 at 3595.838 kb on - strand, within Pf1N1B4_3214 at 3595.838 kb on - strand, within Pf1N1B4_3214 at 3596.122 kb on + strand, within Pf1N1B4_3214 at 3596.222 kb on + strand at 3596.222 kb on + strand, within Pf1N1B4_3214 at 3596.223 kb on - strand, within Pf1N1B4_3214 at 3596.223 kb on - strand, within Pf1N1B4_3214 at 3596.223 kb on - strand, within Pf1N1B4_3214 at 3596.223 kb on - strand at 3596.269 kb on + strand, within Pf1N1B4_3214 at 3596.269 kb on + strand, within Pf1N1B4_3214 at 3596.270 kb on - strand, within Pf1N1B4_3214 at 3596.416 kb on + strand at 3596.416 kb on + strand at 3596.416 kb on + strand at 3596.417 kb on - strand at 3596.417 kb on - strand at 3596.417 kb on - strand at 3596.449 kb on + strand at 3596.449 kb on + strand at 3596.449 kb on + strand at 3596.449 kb on + strand at 3596.450 kb on - strand at 3596.450 kb on - strand at 3596.450 kb on - strand at 3596.450 kb on - strand at 3596.464 kb on + strand at 3596.464 kb on + strand at 3596.810 kb on - strand, within Pf1N1B4_3215 at 3597.368 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 3,594,785 + Pf1N1B4_3212 0.44 -1.3 3,594,786 - Pf1N1B4_3212 0.44 +1.9 3,594,870 - Pf1N1B4_3212 0.56 -1.3 3,594,870 - Pf1N1B4_3212 0.56 +0.2 3,595,148 - -1.3 3,595,227 - Pf1N1B4_3213 0.29 -0.9 3,595,433 - +0.7 3,595,437 - -0.0 3,595,504 - +0.2 3,595,596 - -0.7 3,595,650 + +0.5 3,595,650 + -0.7 3,595,650 + -0.6 3,595,650 + +0.9 3,595,662 - +1.1 3,595,700 + -0.7 3,595,759 + -0.3 3,595,837 + Pf1N1B4_3214 0.13 -1.9 3,595,837 + +0.3 3,595,837 + Pf1N1B4_3214 0.13 +0.5 3,595,838 - Pf1N1B4_3214 0.13 -1.7 3,595,838 - Pf1N1B4_3214 0.13 -0.9 3,596,122 + Pf1N1B4_3214 0.54 -1.0 3,596,222 + +0.7 3,596,222 + Pf1N1B4_3214 0.68 -0.9 3,596,223 - Pf1N1B4_3214 0.68 -0.6 3,596,223 - Pf1N1B4_3214 0.68 -0.3 3,596,223 - Pf1N1B4_3214 0.68 -2.1 3,596,223 - -1.6 3,596,269 + Pf1N1B4_3214 0.75 -0.3 3,596,269 + Pf1N1B4_3214 0.75 -0.0 3,596,270 - Pf1N1B4_3214 0.75 -0.0 3,596,416 + +1.2 3,596,416 + -0.5 3,596,416 + +1.7 3,596,417 - -0.9 3,596,417 - -0.3 3,596,417 - -0.0 3,596,449 + -0.9 3,596,449 + -0.2 3,596,449 + -0.3 3,596,449 + +1.1 3,596,450 - +0.7 3,596,450 - +0.4 3,596,450 - -1.3 3,596,450 - +0.5 3,596,464 + +0.3 3,596,464 + -0.7 3,596,810 - Pf1N1B4_3215 0.47 -0.0 3,597,368 + +0.7
Or see this region's nucleotide sequence