Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3214

Experiment: Gentamicin 0.001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3212 and Pf1N1B4_3213 overlap by 7 nucleotidesPf1N1B4_3213 and Pf1N1B4_3214 are separated by 369 nucleotidesPf1N1B4_3214 and Pf1N1B4_3215 are separated by 0 nucleotidesPf1N1B4_3215 and Pf1N1B4_3216 overlap by 4 nucleotides Pf1N1B4_3212 - hypothetical protein, at 3,594,481 to 3,595,173 _3212 Pf1N1B4_3213 - hypothetical protein, at 3,595,167 to 3,595,376 _3213 Pf1N1B4_3214 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 3,595,746 to 3,596,447 _3214 Pf1N1B4_3215 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 3,596,448 to 3,597,215 _3215 Pf1N1B4_3216 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 3,597,212 to 3,598,468 _3216 Position (kb) 3595 3596 3597Strain fitness (log2 ratio) -2 -1 0 1 2at 3594.785 kb on + strand, within Pf1N1B4_3212at 3594.786 kb on - strand, within Pf1N1B4_3212at 3594.870 kb on - strand, within Pf1N1B4_3212at 3594.870 kb on - strand, within Pf1N1B4_3212at 3595.148 kb on - strandat 3595.227 kb on - strand, within Pf1N1B4_3213at 3595.433 kb on - strandat 3595.437 kb on - strandat 3595.504 kb on - strandat 3595.596 kb on - strandat 3595.650 kb on + strandat 3595.650 kb on + strandat 3595.650 kb on + strandat 3595.650 kb on + strandat 3595.662 kb on - strandat 3595.700 kb on + strandat 3595.759 kb on + strandat 3595.837 kb on + strand, within Pf1N1B4_3214at 3595.837 kb on + strandat 3595.837 kb on + strand, within Pf1N1B4_3214at 3595.838 kb on - strand, within Pf1N1B4_3214at 3595.838 kb on - strand, within Pf1N1B4_3214at 3596.122 kb on + strand, within Pf1N1B4_3214at 3596.222 kb on + strandat 3596.222 kb on + strand, within Pf1N1B4_3214at 3596.223 kb on - strand, within Pf1N1B4_3214at 3596.223 kb on - strand, within Pf1N1B4_3214at 3596.223 kb on - strand, within Pf1N1B4_3214at 3596.223 kb on - strandat 3596.269 kb on + strand, within Pf1N1B4_3214at 3596.269 kb on + strand, within Pf1N1B4_3214at 3596.270 kb on - strand, within Pf1N1B4_3214at 3596.416 kb on + strandat 3596.416 kb on + strandat 3596.416 kb on + strandat 3596.417 kb on - strandat 3596.417 kb on - strandat 3596.417 kb on - strandat 3596.449 kb on + strandat 3596.449 kb on + strandat 3596.449 kb on + strandat 3596.449 kb on + strandat 3596.450 kb on - strandat 3596.450 kb on - strandat 3596.450 kb on - strandat 3596.450 kb on - strandat 3596.464 kb on + strandat 3596.464 kb on + strandat 3596.810 kb on - strand, within Pf1N1B4_3215at 3597.368 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml
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3,594,785 + Pf1N1B4_3212 0.44 -1.3
3,594,786 - Pf1N1B4_3212 0.44 +1.9
3,594,870 - Pf1N1B4_3212 0.56 -1.3
3,594,870 - Pf1N1B4_3212 0.56 +0.2
3,595,148 - -1.3
3,595,227 - Pf1N1B4_3213 0.29 -0.9
3,595,433 - +0.7
3,595,437 - -0.0
3,595,504 - +0.2
3,595,596 - -0.7
3,595,650 + +0.5
3,595,650 + -0.7
3,595,650 + -0.6
3,595,650 + +0.9
3,595,662 - +1.1
3,595,700 + -0.7
3,595,759 + -0.3
3,595,837 + Pf1N1B4_3214 0.13 -1.9
3,595,837 + +0.3
3,595,837 + Pf1N1B4_3214 0.13 +0.5
3,595,838 - Pf1N1B4_3214 0.13 -1.7
3,595,838 - Pf1N1B4_3214 0.13 -0.9
3,596,122 + Pf1N1B4_3214 0.54 -1.0
3,596,222 + +0.7
3,596,222 + Pf1N1B4_3214 0.68 -0.9
3,596,223 - Pf1N1B4_3214 0.68 -0.6
3,596,223 - Pf1N1B4_3214 0.68 -0.3
3,596,223 - Pf1N1B4_3214 0.68 -2.1
3,596,223 - -1.6
3,596,269 + Pf1N1B4_3214 0.75 -0.3
3,596,269 + Pf1N1B4_3214 0.75 -0.0
3,596,270 - Pf1N1B4_3214 0.75 -0.0
3,596,416 + +1.2
3,596,416 + -0.5
3,596,416 + +1.7
3,596,417 - -0.9
3,596,417 - -0.3
3,596,417 - -0.0
3,596,449 + -0.9
3,596,449 + -0.2
3,596,449 + -0.3
3,596,449 + +1.1
3,596,450 - +0.7
3,596,450 - +0.4
3,596,450 - -1.3
3,596,450 - +0.5
3,596,464 + +0.3
3,596,464 + -0.7
3,596,810 - Pf1N1B4_3215 0.47 -0.0
3,597,368 + +0.7

Or see this region's nucleotide sequence