Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3111 and Pf1N1B4_3112 overlap by 13 nucleotides Pf1N1B4_3112 and Pf1N1B4_3113 overlap by 1 nucleotides Pf1N1B4_3113 and Pf1N1B4_3114 are separated by 498 nucleotides
Pf1N1B4_3111 - tRNA pseudouridine 13 synthase (EC 4.2.1.-), at 3,497,707 to 3,498,765
_3111
Pf1N1B4_3112 - 5-nucleotidase SurE (EC 3.1.3.5) @ Exopolyphosphatase (EC 3.6.1.11), at 3,498,753 to 3,499,502
_3112
Pf1N1B4_3113 - Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77), at 3,499,502 to 3,500,179
_3113
Pf1N1B4_3114 - hypothetical protein, at 3,500,678 to 3,500,806
_3114
Position (kb)
3499
3500
3501 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3498.758 kb on + strand at 3498.810 kb on + strand at 3498.810 kb on + strand at 3499.056 kb on + strand at 3499.056 kb on + strand, within Pf1N1B4_3112 at 3499.138 kb on + strand, within Pf1N1B4_3112 at 3499.138 kb on + strand, within Pf1N1B4_3112 at 3499.138 kb on + strand, within Pf1N1B4_3112 at 3499.416 kb on + strand, within Pf1N1B4_3112 at 3499.416 kb on + strand, within Pf1N1B4_3112 at 3499.416 kb on + strand, within Pf1N1B4_3112 at 3499.416 kb on + strand, within Pf1N1B4_3112 at 3499.416 kb on + strand, within Pf1N1B4_3112 at 3499.416 kb on + strand, within Pf1N1B4_3112 at 3499.416 kb on + strand, within Pf1N1B4_3112 at 3499.416 kb on + strand, within Pf1N1B4_3112 at 3499.416 kb on + strand, within Pf1N1B4_3112 at 3499.416 kb on + strand, within Pf1N1B4_3112 at 3499.454 kb on + strand at 3499.522 kb on + strand at 3499.522 kb on + strand at 3499.522 kb on + strand at 3499.522 kb on + strand at 3499.522 kb on + strand at 3499.522 kb on + strand at 3499.523 kb on - strand at 3499.586 kb on + strand, within Pf1N1B4_3113 at 3499.586 kb on + strand, within Pf1N1B4_3113 at 3499.679 kb on + strand, within Pf1N1B4_3113 at 3499.679 kb on + strand, within Pf1N1B4_3113 at 3499.679 kb on + strand, within Pf1N1B4_3113 at 3499.687 kb on + strand, within Pf1N1B4_3113 at 3499.687 kb on + strand, within Pf1N1B4_3113 at 3499.687 kb on + strand, within Pf1N1B4_3113 at 3499.708 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.785 kb on + strand, within Pf1N1B4_3113 at 3499.785 kb on + strand, within Pf1N1B4_3113 at 3499.785 kb on + strand, within Pf1N1B4_3113 at 3499.785 kb on + strand, within Pf1N1B4_3113 at 3499.785 kb on + strand, within Pf1N1B4_3113 at 3499.973 kb on + strand, within Pf1N1B4_3113 at 3499.973 kb on + strand, within Pf1N1B4_3113 at 3499.973 kb on + strand, within Pf1N1B4_3113 at 3499.973 kb on + strand, within Pf1N1B4_3113 at 3500.224 kb on + strand at 3500.224 kb on + strand at 3500.304 kb on + strand at 3500.304 kb on + strand at 3500.325 kb on + strand at 3500.325 kb on + strand at 3500.325 kb on + strand at 3500.325 kb on + strand at 3500.325 kb on + strand at 3500.325 kb on + strand at 3500.325 kb on + strand at 3500.325 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 3,498,758 + +0.8 3,498,810 + -0.2 3,498,810 + -1.9 3,499,056 + +0.2 3,499,056 + Pf1N1B4_3112 0.40 +0.3 3,499,138 + Pf1N1B4_3112 0.51 +2.6 3,499,138 + Pf1N1B4_3112 0.51 -2.2 3,499,138 + Pf1N1B4_3112 0.51 -0.1 3,499,416 + Pf1N1B4_3112 0.88 -1.2 3,499,416 + Pf1N1B4_3112 0.88 +0.7 3,499,416 + Pf1N1B4_3112 0.88 +0.3 3,499,416 + Pf1N1B4_3112 0.88 -0.1 3,499,416 + Pf1N1B4_3112 0.88 -0.1 3,499,416 + Pf1N1B4_3112 0.88 +0.6 3,499,416 + Pf1N1B4_3112 0.88 -0.8 3,499,416 + Pf1N1B4_3112 0.88 -0.1 3,499,416 + Pf1N1B4_3112 0.88 +0.4 3,499,416 + Pf1N1B4_3112 0.88 -1.2 3,499,454 + -0.5 3,499,522 + +0.6 3,499,522 + -0.2 3,499,522 + +0.0 3,499,522 + -2.6 3,499,522 + -0.2 3,499,522 + +2.6 3,499,523 - -1.1 3,499,586 + Pf1N1B4_3113 0.12 +2.8 3,499,586 + Pf1N1B4_3113 0.12 +0.2 3,499,679 + Pf1N1B4_3113 0.26 +2.5 3,499,679 + Pf1N1B4_3113 0.26 +1.8 3,499,679 + Pf1N1B4_3113 0.26 -0.6 3,499,687 + Pf1N1B4_3113 0.27 -0.7 3,499,687 + Pf1N1B4_3113 0.27 +1.1 3,499,687 + Pf1N1B4_3113 0.27 +0.9 3,499,708 + Pf1N1B4_3113 0.30 +1.2 3,499,730 + Pf1N1B4_3113 0.34 -0.1 3,499,730 + Pf1N1B4_3113 0.34 +0.8 3,499,730 + Pf1N1B4_3113 0.34 +1.2 3,499,730 + Pf1N1B4_3113 0.34 -0.7 3,499,730 + Pf1N1B4_3113 0.34 +1.3 3,499,730 + Pf1N1B4_3113 0.34 -0.2 3,499,730 + Pf1N1B4_3113 0.34 +1.2 3,499,730 + Pf1N1B4_3113 0.34 -0.2 3,499,730 + Pf1N1B4_3113 0.34 +1.4 3,499,730 + Pf1N1B4_3113 0.34 +2.1 3,499,730 + Pf1N1B4_3113 0.34 +0.5 3,499,730 + Pf1N1B4_3113 0.34 +1.3 3,499,730 + Pf1N1B4_3113 0.34 -0.2 3,499,730 + Pf1N1B4_3113 0.34 +0.6 3,499,730 + Pf1N1B4_3113 0.34 -1.2 3,499,730 + Pf1N1B4_3113 0.34 -0.1 3,499,730 + Pf1N1B4_3113 0.34 +3.2 3,499,730 + Pf1N1B4_3113 0.34 +1.3 3,499,730 + Pf1N1B4_3113 0.34 +1.4 3,499,730 + Pf1N1B4_3113 0.34 +0.2 3,499,730 + Pf1N1B4_3113 0.34 +1.5 3,499,730 + Pf1N1B4_3113 0.34 -0.5 3,499,730 + Pf1N1B4_3113 0.34 +3.1 3,499,730 + Pf1N1B4_3113 0.34 +1.6 3,499,730 + Pf1N1B4_3113 0.34 +0.8 3,499,730 + Pf1N1B4_3113 0.34 +0.4 3,499,730 + Pf1N1B4_3113 0.34 +1.1 3,499,730 + Pf1N1B4_3113 0.34 +0.8 3,499,730 + Pf1N1B4_3113 0.34 +0.2 3,499,730 + Pf1N1B4_3113 0.34 +1.2 3,499,730 + Pf1N1B4_3113 0.34 +0.3 3,499,730 + Pf1N1B4_3113 0.34 -1.2 3,499,730 + Pf1N1B4_3113 0.34 -0.8 3,499,730 + Pf1N1B4_3113 0.34 +1.1 3,499,730 + Pf1N1B4_3113 0.34 +2.0 3,499,730 + Pf1N1B4_3113 0.34 +1.1 3,499,730 + Pf1N1B4_3113 0.34 -1.0 3,499,730 + +0.0 3,499,730 + Pf1N1B4_3113 0.34 +0.3 3,499,730 + Pf1N1B4_3113 0.34 +0.1 3,499,730 + Pf1N1B4_3113 0.34 +0.0 3,499,730 + Pf1N1B4_3113 0.34 +0.5 3,499,730 + Pf1N1B4_3113 0.34 +0.3 3,499,730 + Pf1N1B4_3113 0.34 -1.1 3,499,730 + Pf1N1B4_3113 0.34 +0.9 3,499,730 + Pf1N1B4_3113 0.34 -0.1 3,499,730 + Pf1N1B4_3113 0.34 -0.2 3,499,730 + Pf1N1B4_3113 0.34 +0.8 3,499,732 + Pf1N1B4_3113 0.34 +0.8 3,499,732 + Pf1N1B4_3113 0.34 +1.6 3,499,732 + Pf1N1B4_3113 0.34 +2.1 3,499,732 + Pf1N1B4_3113 0.34 +1.6 3,499,732 + Pf1N1B4_3113 0.34 +0.3 3,499,732 + Pf1N1B4_3113 0.34 +0.7 3,499,732 + Pf1N1B4_3113 0.34 +0.6 3,499,732 + Pf1N1B4_3113 0.34 +1.9 3,499,732 + Pf1N1B4_3113 0.34 +0.6 3,499,732 + +1.2 3,499,732 + Pf1N1B4_3113 0.34 +0.7 3,499,732 + Pf1N1B4_3113 0.34 +0.8 3,499,732 + Pf1N1B4_3113 0.34 +1.1 3,499,732 + Pf1N1B4_3113 0.34 +1.3 3,499,732 + Pf1N1B4_3113 0.34 +0.3 3,499,732 + Pf1N1B4_3113 0.34 +1.5 3,499,732 + Pf1N1B4_3113 0.34 -1.2 3,499,732 + Pf1N1B4_3113 0.34 +0.5 3,499,732 + Pf1N1B4_3113 0.34 -0.1 3,499,732 + Pf1N1B4_3113 0.34 +1.5 3,499,732 + Pf1N1B4_3113 0.34 +0.6 3,499,732 + Pf1N1B4_3113 0.34 +1.2 3,499,732 + Pf1N1B4_3113 0.34 +2.0 3,499,732 + Pf1N1B4_3113 0.34 -0.5 3,499,732 + Pf1N1B4_3113 0.34 +0.0 3,499,732 + Pf1N1B4_3113 0.34 +1.2 3,499,785 + Pf1N1B4_3113 0.42 -1.0 3,499,785 + Pf1N1B4_3113 0.42 +0.8 3,499,785 + Pf1N1B4_3113 0.42 +0.5 3,499,785 + Pf1N1B4_3113 0.42 +0.2 3,499,785 + Pf1N1B4_3113 0.42 +1.6 3,499,973 + Pf1N1B4_3113 0.69 +0.8 3,499,973 + Pf1N1B4_3113 0.69 +0.5 3,499,973 + Pf1N1B4_3113 0.69 +1.1 3,499,973 + Pf1N1B4_3113 0.69 +0.8 3,500,224 + -0.2 3,500,224 + +0.5 3,500,304 + +1.1 3,500,304 + +1.8 3,500,325 + +0.4 3,500,325 + +0.8 3,500,325 + +1.5 3,500,325 + +1.3 3,500,325 + +0.6 3,500,325 + +1.0 3,500,325 + +0.3 3,500,325 + +0.2
Or see this region's nucleotide sequence