Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3027 and Pf1N1B4_3028 are separated by 109 nucleotides Pf1N1B4_3028 and Pf1N1B4_3029 are separated by 90 nucleotides
Pf1N1B4_3027 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 3,404,317 to 3,405,435
_3027
Pf1N1B4_3028 - ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1), at 3,405,545 to 3,406,696
_3028
Pf1N1B4_3029 - 4-aminobutyraldehyde dehydrogenase (EC 1.2.1.19), at 3,406,787 to 3,408,244
_3029
Position (kb)
3405
3406
3407 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3404.687 kb on + strand, within Pf1N1B4_3027 at 3404.687 kb on + strand, within Pf1N1B4_3027 at 3404.687 kb on + strand, within Pf1N1B4_3027 at 3404.687 kb on + strand, within Pf1N1B4_3027 at 3404.688 kb on - strand, within Pf1N1B4_3027 at 3404.964 kb on + strand, within Pf1N1B4_3027 at 3405.076 kb on + strand, within Pf1N1B4_3027 at 3405.076 kb on + strand, within Pf1N1B4_3027 at 3405.076 kb on + strand, within Pf1N1B4_3027 at 3405.077 kb on - strand, within Pf1N1B4_3027 at 3405.077 kb on - strand, within Pf1N1B4_3027 at 3405.117 kb on + strand, within Pf1N1B4_3027 at 3405.117 kb on + strand at 3405.118 kb on - strand, within Pf1N1B4_3027 at 3405.118 kb on - strand, within Pf1N1B4_3027 at 3405.118 kb on - strand, within Pf1N1B4_3027 at 3405.118 kb on - strand, within Pf1N1B4_3027 at 3405.168 kb on + strand, within Pf1N1B4_3027 at 3405.560 kb on - strand at 3405.570 kb on + strand at 3405.570 kb on + strand at 3405.570 kb on + strand at 3405.570 kb on + strand at 3405.570 kb on + strand at 3405.570 kb on + strand at 3405.570 kb on + strand at 3405.571 kb on - strand at 3405.571 kb on - strand at 3405.571 kb on - strand at 3405.571 kb on - strand at 3405.571 kb on - strand at 3405.571 kb on - strand at 3405.571 kb on - strand at 3405.571 kb on - strand at 3405.571 kb on - strand at 3405.590 kb on - strand at 3405.591 kb on + strand at 3405.591 kb on + strand at 3405.591 kb on + strand at 3405.592 kb on - strand at 3405.592 kb on - strand at 3405.703 kb on + strand, within Pf1N1B4_3028 at 3405.704 kb on - strand, within Pf1N1B4_3028 at 3405.792 kb on + strand at 3405.793 kb on - strand, within Pf1N1B4_3028 at 3406.038 kb on + strand, within Pf1N1B4_3028 at 3406.039 kb on - strand, within Pf1N1B4_3028 at 3406.039 kb on - strand, within Pf1N1B4_3028 at 3406.059 kb on + strand, within Pf1N1B4_3028 at 3406.101 kb on + strand, within Pf1N1B4_3028 at 3406.101 kb on + strand, within Pf1N1B4_3028 at 3406.101 kb on + strand, within Pf1N1B4_3028 at 3406.122 kb on + strand, within Pf1N1B4_3028 at 3406.122 kb on + strand, within Pf1N1B4_3028 at 3406.122 kb on + strand, within Pf1N1B4_3028 at 3406.122 kb on + strand, within Pf1N1B4_3028 at 3406.123 kb on - strand, within Pf1N1B4_3028 at 3406.123 kb on - strand, within Pf1N1B4_3028 at 3406.123 kb on - strand, within Pf1N1B4_3028 at 3406.137 kb on + strand, within Pf1N1B4_3028 at 3406.138 kb on - strand, within Pf1N1B4_3028 at 3406.138 kb on - strand, within Pf1N1B4_3028 at 3406.138 kb on - strand, within Pf1N1B4_3028 at 3406.210 kb on + strand at 3406.210 kb on + strand, within Pf1N1B4_3028 at 3406.211 kb on - strand at 3406.211 kb on - strand, within Pf1N1B4_3028 at 3406.239 kb on + strand, within Pf1N1B4_3028 at 3406.240 kb on - strand, within Pf1N1B4_3028 at 3406.240 kb on - strand, within Pf1N1B4_3028 at 3406.240 kb on - strand at 3406.240 kb on - strand at 3406.240 kb on - strand, within Pf1N1B4_3028 at 3406.240 kb on - strand, within Pf1N1B4_3028 at 3406.240 kb on - strand, within Pf1N1B4_3028 at 3406.240 kb on - strand at 3406.240 kb on - strand, within Pf1N1B4_3028 at 3406.279 kb on - strand, within Pf1N1B4_3028 at 3406.297 kb on + strand at 3406.297 kb on + strand, within Pf1N1B4_3028 at 3406.297 kb on + strand, within Pf1N1B4_3028 at 3406.297 kb on + strand, within Pf1N1B4_3028 at 3406.297 kb on + strand, within Pf1N1B4_3028 at 3406.297 kb on + strand, within Pf1N1B4_3028 at 3406.297 kb on + strand, within Pf1N1B4_3028 at 3406.297 kb on + strand, within Pf1N1B4_3028 at 3406.297 kb on + strand at 3406.298 kb on - strand, within Pf1N1B4_3028 at 3406.299 kb on + strand, within Pf1N1B4_3028 at 3406.299 kb on + strand, within Pf1N1B4_3028 at 3406.299 kb on + strand, within Pf1N1B4_3028 at 3406.299 kb on + strand at 3406.299 kb on + strand, within Pf1N1B4_3028 at 3406.299 kb on + strand, within Pf1N1B4_3028 at 3406.299 kb on + strand, within Pf1N1B4_3028 at 3406.299 kb on + strand, within Pf1N1B4_3028 at 3406.299 kb on + strand, within Pf1N1B4_3028 at 3406.300 kb on - strand at 3406.300 kb on - strand, within Pf1N1B4_3028 at 3406.300 kb on - strand, within Pf1N1B4_3028 at 3406.300 kb on - strand, within Pf1N1B4_3028 at 3406.300 kb on - strand, within Pf1N1B4_3028 at 3406.300 kb on - strand, within Pf1N1B4_3028 at 3406.300 kb on - strand, within Pf1N1B4_3028 at 3406.358 kb on - strand, within Pf1N1B4_3028 at 3406.358 kb on - strand, within Pf1N1B4_3028 at 3406.560 kb on + strand, within Pf1N1B4_3028 at 3406.561 kb on - strand at 3406.714 kb on - strand at 3406.714 kb on - strand at 3406.714 kb on - strand at 3406.714 kb on - strand at 3406.718 kb on + strand at 3406.718 kb on + strand at 3406.719 kb on - strand at 3406.773 kb on - strand at 3406.788 kb on + strand at 3406.789 kb on - strand at 3406.789 kb on - strand at 3406.789 kb on - strand at 3406.789 kb on - strand at 3406.790 kb on + strand at 3406.790 kb on + strand at 3406.790 kb on + strand at 3406.790 kb on + strand at 3406.791 kb on - strand at 3406.791 kb on - strand at 3406.791 kb on - strand at 3406.791 kb on - strand at 3406.833 kb on + strand at 3406.833 kb on + strand at 3406.833 kb on + strand at 3406.833 kb on + strand at 3406.834 kb on - strand at 3406.834 kb on - strand at 3406.856 kb on + strand at 3406.857 kb on - strand at 3407.145 kb on + strand, within Pf1N1B4_3029 at 3407.145 kb on + strand at 3407.146 kb on - strand, within Pf1N1B4_3029 at 3407.148 kb on + strand, within Pf1N1B4_3029 at 3407.148 kb on + strand, within Pf1N1B4_3029 at 3407.148 kb on + strand, within Pf1N1B4_3029 at 3407.148 kb on + strand, within Pf1N1B4_3029 at 3407.148 kb on + strand, within Pf1N1B4_3029 at 3407.148 kb on + strand, within Pf1N1B4_3029 at 3407.149 kb on - strand, within Pf1N1B4_3029 at 3407.149 kb on - strand, within Pf1N1B4_3029 at 3407.364 kb on + strand at 3407.364 kb on + strand, within Pf1N1B4_3029 at 3407.364 kb on + strand, within Pf1N1B4_3029 at 3407.365 kb on - strand, within Pf1N1B4_3029 at 3407.365 kb on - strand, within Pf1N1B4_3029 at 3407.365 kb on - strand, within Pf1N1B4_3029 at 3407.403 kb on + strand, within Pf1N1B4_3029 at 3407.403 kb on + strand, within Pf1N1B4_3029 at 3407.403 kb on + strand, within Pf1N1B4_3029 at 3407.403 kb on + strand, within Pf1N1B4_3029 at 3407.403 kb on + strand, within Pf1N1B4_3029 at 3407.404 kb on - strand, within Pf1N1B4_3029 at 3407.404 kb on - strand, within Pf1N1B4_3029 at 3407.404 kb on - strand, within Pf1N1B4_3029 at 3407.404 kb on - strand, within Pf1N1B4_3029 at 3407.404 kb on - strand, within Pf1N1B4_3029 at 3407.406 kb on + strand, within Pf1N1B4_3029 at 3407.407 kb on - strand, within Pf1N1B4_3029 at 3407.412 kb on + strand, within Pf1N1B4_3029 at 3407.413 kb on - strand, within Pf1N1B4_3029 at 3407.413 kb on - strand, within Pf1N1B4_3029 at 3407.413 kb on - strand, within Pf1N1B4_3029 at 3407.413 kb on - strand, within Pf1N1B4_3029 at 3407.413 kb on - strand, within Pf1N1B4_3029 at 3407.591 kb on + strand, within Pf1N1B4_3029 at 3407.591 kb on + strand, within Pf1N1B4_3029 at 3407.592 kb on - strand, within Pf1N1B4_3029 at 3407.592 kb on - strand, within Pf1N1B4_3029 at 3407.626 kb on + strand, within Pf1N1B4_3029 at 3407.626 kb on + strand, within Pf1N1B4_3029 at 3407.626 kb on + strand, within Pf1N1B4_3029 at 3407.626 kb on + strand, within Pf1N1B4_3029 at 3407.626 kb on + strand, within Pf1N1B4_3029 at 3407.627 kb on - strand, within Pf1N1B4_3029 at 3407.627 kb on - strand, within Pf1N1B4_3029 at 3407.627 kb on - strand, within Pf1N1B4_3029 at 3407.627 kb on - strand, within Pf1N1B4_3029
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 3,404,687 + Pf1N1B4_3027 0.33 -1.3 3,404,687 + Pf1N1B4_3027 0.33 +3.4 3,404,687 + Pf1N1B4_3027 0.33 -1.8 3,404,687 + Pf1N1B4_3027 0.33 -1.5 3,404,688 - Pf1N1B4_3027 0.33 +1.2 3,404,964 + Pf1N1B4_3027 0.58 -0.4 3,405,076 + Pf1N1B4_3027 0.68 -0.2 3,405,076 + Pf1N1B4_3027 0.68 -2.7 3,405,076 + Pf1N1B4_3027 0.68 -0.9 3,405,077 - Pf1N1B4_3027 0.68 -1.2 3,405,077 - Pf1N1B4_3027 0.68 -0.8 3,405,117 + Pf1N1B4_3027 0.71 +0.2 3,405,117 + +0.8 3,405,118 - Pf1N1B4_3027 0.72 -0.1 3,405,118 - Pf1N1B4_3027 0.72 +0.6 3,405,118 - Pf1N1B4_3027 0.72 +1.2 3,405,118 - Pf1N1B4_3027 0.72 -0.2 3,405,168 + Pf1N1B4_3027 0.76 -1.5 3,405,560 - +0.5 3,405,570 + -0.8 3,405,570 + +0.8 3,405,570 + -2.0 3,405,570 + -0.5 3,405,570 + -0.3 3,405,570 + -0.2 3,405,570 + +0.3 3,405,571 - -1.5 3,405,571 - -2.9 3,405,571 - +0.9 3,405,571 - +0.8 3,405,571 - -0.2 3,405,571 - -1.0 3,405,571 - -0.9 3,405,571 - -2.2 3,405,571 - -1.8 3,405,590 - +0.3 3,405,591 + -0.8 3,405,591 + +0.2 3,405,591 + +0.2 3,405,592 - +1.4 3,405,592 - +0.8 3,405,703 + Pf1N1B4_3028 0.14 +0.3 3,405,704 - Pf1N1B4_3028 0.14 +0.5 3,405,792 + +0.8 3,405,793 - Pf1N1B4_3028 0.22 -2.2 3,406,038 + Pf1N1B4_3028 0.43 -1.4 3,406,039 - Pf1N1B4_3028 0.43 -1.2 3,406,039 - Pf1N1B4_3028 0.43 -0.7 3,406,059 + Pf1N1B4_3028 0.45 +0.2 3,406,101 + Pf1N1B4_3028 0.48 -1.5 3,406,101 + Pf1N1B4_3028 0.48 +0.8 3,406,101 + Pf1N1B4_3028 0.48 -0.0 3,406,122 + Pf1N1B4_3028 0.50 +1.3 3,406,122 + Pf1N1B4_3028 0.50 -0.0 3,406,122 + Pf1N1B4_3028 0.50 -0.8 3,406,122 + Pf1N1B4_3028 0.50 +0.2 3,406,123 - Pf1N1B4_3028 0.50 +0.1 3,406,123 - Pf1N1B4_3028 0.50 -1.1 3,406,123 - Pf1N1B4_3028 0.50 -0.4 3,406,137 + Pf1N1B4_3028 0.51 +0.1 3,406,138 - Pf1N1B4_3028 0.51 -0.5 3,406,138 - Pf1N1B4_3028 0.51 -1.5 3,406,138 - Pf1N1B4_3028 0.51 -2.0 3,406,210 + +2.8 3,406,210 + Pf1N1B4_3028 0.58 +0.3 3,406,211 - +0.2 3,406,211 - Pf1N1B4_3028 0.58 +0.5 3,406,239 + Pf1N1B4_3028 0.60 +2.6 3,406,240 - Pf1N1B4_3028 0.60 -1.5 3,406,240 - Pf1N1B4_3028 0.60 +0.1 3,406,240 - -1.2 3,406,240 - -0.2 3,406,240 - Pf1N1B4_3028 0.60 +2.6 3,406,240 - Pf1N1B4_3028 0.60 -0.2 3,406,240 - Pf1N1B4_3028 0.60 -0.1 3,406,240 - -0.8 3,406,240 - Pf1N1B4_3028 0.60 -0.3 3,406,279 - Pf1N1B4_3028 0.64 -2.4 3,406,297 + +1.8 3,406,297 + Pf1N1B4_3028 0.65 -0.0 3,406,297 + Pf1N1B4_3028 0.65 -1.7 3,406,297 + Pf1N1B4_3028 0.65 +0.8 3,406,297 + Pf1N1B4_3028 0.65 +1.1 3,406,297 + Pf1N1B4_3028 0.65 -0.5 3,406,297 + Pf1N1B4_3028 0.65 -1.4 3,406,297 + Pf1N1B4_3028 0.65 +1.0 3,406,297 + -0.8 3,406,298 - Pf1N1B4_3028 0.65 +0.8 3,406,299 + Pf1N1B4_3028 0.65 +0.1 3,406,299 + Pf1N1B4_3028 0.65 +0.5 3,406,299 + Pf1N1B4_3028 0.65 +1.4 3,406,299 + -0.2 3,406,299 + Pf1N1B4_3028 0.65 +1.8 3,406,299 + Pf1N1B4_3028 0.65 -0.2 3,406,299 + Pf1N1B4_3028 0.65 -0.1 3,406,299 + Pf1N1B4_3028 0.65 +0.1 3,406,299 + Pf1N1B4_3028 0.65 +0.8 3,406,300 - -0.2 3,406,300 - Pf1N1B4_3028 0.66 -1.2 3,406,300 - Pf1N1B4_3028 0.66 +0.2 3,406,300 - Pf1N1B4_3028 0.66 -0.5 3,406,300 - Pf1N1B4_3028 0.66 -1.5 3,406,300 - Pf1N1B4_3028 0.66 +1.1 3,406,300 - Pf1N1B4_3028 0.66 +0.1 3,406,358 - Pf1N1B4_3028 0.71 -1.0 3,406,358 - Pf1N1B4_3028 0.71 +0.1 3,406,560 + Pf1N1B4_3028 0.88 -0.4 3,406,561 - +2.2 3,406,714 - +0.4 3,406,714 - -0.2 3,406,714 - -2.9 3,406,714 - -0.9 3,406,718 + +1.1 3,406,718 + -0.5 3,406,719 - -0.8 3,406,773 - -2.0 3,406,788 + +0.1 3,406,789 - +0.8 3,406,789 - -0.3 3,406,789 - +0.1 3,406,789 - -1.5 3,406,790 + -1.0 3,406,790 + +0.2 3,406,790 + -0.4 3,406,790 + -0.2 3,406,791 - -1.8 3,406,791 - -0.6 3,406,791 - -2.2 3,406,791 - +0.8 3,406,833 + +0.1 3,406,833 + -0.2 3,406,833 + -0.5 3,406,833 + -1.2 3,406,834 - -0.8 3,406,834 - -0.1 3,406,856 + -0.8 3,406,857 - +0.8 3,407,145 + Pf1N1B4_3029 0.25 -1.0 3,407,145 + -0.8 3,407,146 - Pf1N1B4_3029 0.25 -0.0 3,407,148 + Pf1N1B4_3029 0.25 -0.9 3,407,148 + Pf1N1B4_3029 0.25 +0.2 3,407,148 + Pf1N1B4_3029 0.25 +0.5 3,407,148 + Pf1N1B4_3029 0.25 -0.4 3,407,148 + Pf1N1B4_3029 0.25 -1.2 3,407,148 + Pf1N1B4_3029 0.25 +0.4 3,407,149 - Pf1N1B4_3029 0.25 +0.8 3,407,149 - Pf1N1B4_3029 0.25 +0.2 3,407,364 + +0.8 3,407,364 + Pf1N1B4_3029 0.40 +0.4 3,407,364 + Pf1N1B4_3029 0.40 -2.4 3,407,365 - Pf1N1B4_3029 0.40 +1.5 3,407,365 - Pf1N1B4_3029 0.40 -0.8 3,407,365 - Pf1N1B4_3029 0.40 -0.5 3,407,403 + Pf1N1B4_3029 0.42 +0.1 3,407,403 + Pf1N1B4_3029 0.42 +1.8 3,407,403 + Pf1N1B4_3029 0.42 +0.8 3,407,403 + Pf1N1B4_3029 0.42 +1.2 3,407,403 + Pf1N1B4_3029 0.42 +0.4 3,407,404 - Pf1N1B4_3029 0.42 -0.5 3,407,404 - Pf1N1B4_3029 0.42 +0.2 3,407,404 - Pf1N1B4_3029 0.42 -0.6 3,407,404 - Pf1N1B4_3029 0.42 -0.5 3,407,404 - Pf1N1B4_3029 0.42 +1.4 3,407,406 + Pf1N1B4_3029 0.42 +1.0 3,407,407 - Pf1N1B4_3029 0.43 -0.8 3,407,412 + Pf1N1B4_3029 0.43 +2.2 3,407,413 - Pf1N1B4_3029 0.43 -0.6 3,407,413 - Pf1N1B4_3029 0.43 -0.7 3,407,413 - Pf1N1B4_3029 0.43 -1.7 3,407,413 - Pf1N1B4_3029 0.43 +0.1 3,407,413 - Pf1N1B4_3029 0.43 -0.6 3,407,591 + Pf1N1B4_3029 0.55 +0.3 3,407,591 + Pf1N1B4_3029 0.55 -0.2 3,407,592 - Pf1N1B4_3029 0.55 -0.2 3,407,592 - Pf1N1B4_3029 0.55 -2.2 3,407,626 + Pf1N1B4_3029 0.58 +1.4 3,407,626 + Pf1N1B4_3029 0.58 +0.2 3,407,626 + Pf1N1B4_3029 0.58 +0.7 3,407,626 + Pf1N1B4_3029 0.58 -1.0 3,407,626 + Pf1N1B4_3029 0.58 +0.0 3,407,627 - Pf1N1B4_3029 0.58 +2.3 3,407,627 - Pf1N1B4_3029 0.58 +1.8 3,407,627 - Pf1N1B4_3029 0.58 -1.7 3,407,627 - Pf1N1B4_3029 0.58 +1.1
Or see this region's nucleotide sequence