Experiment: Gentamicin 0.001 mg/ml 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf1N1B4_3024 and Pf1N1B4_3025 are separated by 175 nucleotides Pf1N1B4_3025 and Pf1N1B4_3026 overlap by 11 nucleotides Pf1N1B4_3026 and Pf1N1B4_3027 are separated by 1 nucleotides 
        Pf1N1B4_3024 - 4-aminobutyraldehyde dehydrogenase (EC 1.2.1.19), at 3,400,954 to 3,402,378 
        _3024 
         
        
        Pf1N1B4_3025 - Spermidine Putrescine ABC transporter permease component potC (TC_3.A.1.11.1), at 3,402,554 to 3,403,378 
        _3025 
         
        
        Pf1N1B4_3026 - Spermidine Putrescine ABC transporter permease component PotB (TC 3.A.1.11.1), at 3,403,368 to 3,404,315 
        _3026 
         
        
        Pf1N1B4_3027 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 3,404,317 to 3,405,435 
        _3027 
         Position (kb) 3403 
3404 
3405 Strain fitness (log2 ratio) -3 
-2 
-1 
0 
1 
2 
3 at 3402.382 kb on + strand at 3402.382 kb on + strand at 3402.382 kb on + strand at 3402.382 kb on + strand at 3402.382 kb on + strand at 3402.382 kb on + strand at 3402.383 kb on - strand at 3402.383 kb on - strand at 3402.383 kb on - strand at 3402.383 kb on - strand at 3402.383 kb on - strand at 3402.383 kb on - strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.486 kb on + strand at 3402.486 kb on + strand at 3402.487 kb on - strand at 3402.487 kb on - strand at 3402.487 kb on - strand at 3402.487 kb on - strand at 3402.555 kb on + strand at 3402.600 kb on + strand at 3402.600 kb on + strand at 3402.600 kb on + strand at 3402.603 kb on + strand at 3402.603 kb on + strand at 3402.603 kb on + strand at 3402.603 kb on + strand at 3402.604 kb on - strand at 3402.825 kb on + strand, within Pf1N1B4_3025 at 3402.825 kb on + strand, within Pf1N1B4_3025 at 3402.882 kb on + strand, within Pf1N1B4_3025 at 3402.883 kb on - strand, within Pf1N1B4_3025 at 3402.883 kb on - strand, within Pf1N1B4_3025 at 3402.883 kb on - strand, within Pf1N1B4_3025 at 3402.883 kb on - strand at 3402.883 kb on - strand, within Pf1N1B4_3025 at 3402.883 kb on - strand, within Pf1N1B4_3025 at 3402.918 kb on + strand, within Pf1N1B4_3025 at 3402.918 kb on + strand, within Pf1N1B4_3025 at 3402.919 kb on - strand, within Pf1N1B4_3025 at 3403.335 kb on - strand at 3403.366 kb on + strand at 3403.366 kb on + strand at 3403.366 kb on + strand at 3403.367 kb on - strand at 3403.367 kb on - strand at 3403.367 kb on - strand at 3403.411 kb on + strand at 3403.411 kb on + strand at 3403.411 kb on + strand at 3403.411 kb on + strand at 3403.411 kb on + strand at 3403.411 kb on + strand at 3403.412 kb on - strand at 3403.412 kb on - strand at 3403.412 kb on - strand at 3403.412 kb on - strand at 3403.463 kb on + strand, within Pf1N1B4_3026 at 3403.489 kb on + strand, within Pf1N1B4_3026 at 3403.490 kb on - strand, within Pf1N1B4_3026 at 3403.665 kb on + strand, within Pf1N1B4_3026 at 3403.666 kb on - strand, within Pf1N1B4_3026 at 3403.666 kb on - strand, within Pf1N1B4_3026 at 3403.732 kb on + strand, within Pf1N1B4_3026 at 3403.733 kb on - strand, within Pf1N1B4_3026 at 3403.733 kb on - strand, within Pf1N1B4_3026 at 3403.888 kb on + strand, within Pf1N1B4_3026 at 3403.903 kb on + strand, within Pf1N1B4_3026 at 3403.903 kb on + strand, within Pf1N1B4_3026 at 3403.903 kb on + strand, within Pf1N1B4_3026 at 3403.904 kb on - strand, within Pf1N1B4_3026 at 3403.904 kb on - strand, within Pf1N1B4_3026 at 3403.904 kb on - strand, within Pf1N1B4_3026 at 3403.936 kb on + strand, within Pf1N1B4_3026 at 3403.967 kb on - strand, within Pf1N1B4_3026 at 3403.978 kb on + strand, within Pf1N1B4_3026 at 3403.978 kb on + strand, within Pf1N1B4_3026 at 3403.978 kb on + strand, within Pf1N1B4_3026 at 3403.978 kb on + strand, within Pf1N1B4_3026 at 3403.978 kb on + strand, within Pf1N1B4_3026 at 3403.978 kb on + strand, within Pf1N1B4_3026 at 3403.978 kb on + strand, within Pf1N1B4_3026 at 3403.978 kb on + strand at 3403.979 kb on - strand, within Pf1N1B4_3026 at 3403.979 kb on - strand, within Pf1N1B4_3026 at 3403.979 kb on - strand, within Pf1N1B4_3026 at 3403.979 kb on - strand, within Pf1N1B4_3026 at 3403.979 kb on - strand, within Pf1N1B4_3026 at 3403.982 kb on - strand, within Pf1N1B4_3026 at 3403.982 kb on - strand, within Pf1N1B4_3026 at 3404.140 kb on + strand, within Pf1N1B4_3026 at 3404.140 kb on + strand, within Pf1N1B4_3026 at 3404.140 kb on + strand, within Pf1N1B4_3026 at 3404.140 kb on + strand, within Pf1N1B4_3026 at 3404.141 kb on - strand at 3404.179 kb on + strand, within Pf1N1B4_3026 at 3404.179 kb on + strand, within Pf1N1B4_3026 at 3404.227 kb on + strand at 3404.227 kb on + strand at 3404.227 kb on + strand at 3404.228 kb on - strand at 3404.228 kb on - strand at 3404.245 kb on + strand at 3404.687 kb on + strand, within Pf1N1B4_3027 at 3404.687 kb on + strand, within Pf1N1B4_3027 at 3404.687 kb on + strand, within Pf1N1B4_3027 at 3404.687 kb on + strand, within Pf1N1B4_3027 at 3404.688 kb on - strand, within Pf1N1B4_3027 at 3404.964 kb on + strand, within Pf1N1B4_3027 at 3405.076 kb on + strand, within Pf1N1B4_3027 at 3405.076 kb on + strand, within Pf1N1B4_3027 at 3405.076 kb on + strand, within Pf1N1B4_3027 at 3405.077 kb on - strand, within Pf1N1B4_3027 at 3405.077 kb on - strand, within Pf1N1B4_3027 at 3405.117 kb on + strand, within Pf1N1B4_3027 at 3405.117 kb on + strand at 3405.118 kb on - strand, within Pf1N1B4_3027 at 3405.118 kb on - strand, within Pf1N1B4_3027 at 3405.118 kb on - strand, within Pf1N1B4_3027 at 3405.118 kb on - strand, within Pf1N1B4_3027 at 3405.168 kb on + strand, within Pf1N1B4_3027  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction Gentamicin 0.001 mg/ml remove 3,402,382 +  -1.0 3,402,382 +  -0.8 3,402,382 +  -0.1 3,402,382 +  -0.5 3,402,382 +  -0.4 3,402,382 +  -0.5 3,402,383 -  +0.3 3,402,383 -  +1.1 3,402,383 -  +0.8 3,402,383 -  +0.2 3,402,383 -  +1.8 3,402,383 -  +1.5 3,402,403 +  -2.8 3,402,403 +  +1.8 3,402,403 +  +0.1 3,402,403 +  -0.2 3,402,403 +  -2.2 3,402,403 +  -0.2 3,402,403 +  +1.8 3,402,403 +  -0.4 3,402,403 +  -0.7 3,402,403 +  -0.2 3,402,403 +  -0.8 3,402,403 +  +0.1 3,402,403 +  -0.8 3,402,403 +  -1.0 3,402,403 +  +1.8 3,402,403 +  +0.8 3,402,403 +  +0.5 3,402,404 -  -1.5 3,402,404 -  -0.8 3,402,404 -  -0.8 3,402,404 -  +0.1 3,402,404 -  -1.5 3,402,404 -  +0.1 3,402,404 -  -0.3 3,402,404 -  -0.4 3,402,404 -  -0.0 3,402,404 -  +0.7 3,402,404 -  -2.0 3,402,404 -  -0.8 3,402,404 -  -0.8 3,402,404 -  +1.4 3,402,404 -  -0.4 3,402,486 +  +0.7 3,402,486 +  +0.2 3,402,487 -  +1.4 3,402,487 -  -0.2 3,402,487 -  -2.0 3,402,487 -  -2.4 3,402,555 +  +0.5 3,402,600 +  -1.4 3,402,600 +  -0.4 3,402,600 +  +0.1 3,402,603 +  -0.2 3,402,603 +  -0.4 3,402,603 +  +0.8 3,402,603 +  -0.2 3,402,604 -  +0.8 3,402,825 +  Pf1N1B4_3025 0.33  +1.6 3,402,825 +  Pf1N1B4_3025 0.33  -0.9 3,402,882 +  Pf1N1B4_3025 0.40  -1.9 3,402,883 -  Pf1N1B4_3025 0.40  +0.6 3,402,883 -  Pf1N1B4_3025 0.40  +0.2 3,402,883 -  Pf1N1B4_3025 0.40  -1.0 3,402,883 -  +0.8 3,402,883 -  Pf1N1B4_3025 0.40  -2.5 3,402,883 -  Pf1N1B4_3025 0.40  -0.2 3,402,918 +  Pf1N1B4_3025 0.44  +0.4 3,402,918 +  Pf1N1B4_3025 0.44  +0.1 3,402,919 -  Pf1N1B4_3025 0.44  -1.2 3,403,335 -  +1.8 3,403,366 +  -0.0 3,403,366 +  +0.2 3,403,366 +  +1.4 3,403,367 -  +0.8 3,403,367 -  -2.0 3,403,367 -  +0.8 3,403,411 +  -1.2 3,403,411 +  +0.3 3,403,411 +  +2.4 3,403,411 +  -0.3 3,403,411 +  -0.8 3,403,411 +  +0.8 3,403,412 -  -2.0 3,403,412 -  -0.9 3,403,412 -  -0.8 3,403,412 -  +0.5 3,403,463 +  Pf1N1B4_3026 0.10  -0.2 3,403,489 +  Pf1N1B4_3026 0.13  +0.8 3,403,490 -  Pf1N1B4_3026 0.13  -0.4 3,403,665 +  Pf1N1B4_3026 0.31  +0.8 3,403,666 -  Pf1N1B4_3026 0.31  -0.4 3,403,666 -  Pf1N1B4_3026 0.31  -0.5 3,403,732 +  Pf1N1B4_3026 0.38  -1.7 3,403,733 -  Pf1N1B4_3026 0.39  -2.7 3,403,733 -  Pf1N1B4_3026 0.39  +0.8 3,403,888 +  Pf1N1B4_3026 0.55  -0.9 3,403,903 +  Pf1N1B4_3026 0.56  -1.2 3,403,903 +  Pf1N1B4_3026 0.56  -0.7 3,403,903 +  Pf1N1B4_3026 0.56  +0.8 3,403,904 -  Pf1N1B4_3026 0.57  -0.4 3,403,904 -  Pf1N1B4_3026 0.57  +0.1 3,403,904 -  Pf1N1B4_3026 0.57  -2.7 3,403,936 +  Pf1N1B4_3026 0.60  -0.2 3,403,967 -  Pf1N1B4_3026 0.63  -1.0 3,403,978 +  Pf1N1B4_3026 0.64  +0.8 3,403,978 +  Pf1N1B4_3026 0.64  -0.1 3,403,978 +  Pf1N1B4_3026 0.64  -1.2 3,403,978 +  Pf1N1B4_3026 0.64  -0.0 3,403,978 +  Pf1N1B4_3026 0.64  -0.0 3,403,978 +  Pf1N1B4_3026 0.64  +1.0 3,403,978 +  Pf1N1B4_3026 0.64  +0.8 3,403,978 +  -0.8 3,403,979 -  Pf1N1B4_3026 0.64  -0.3 3,403,979 -  Pf1N1B4_3026 0.64  +1.5 3,403,979 -  Pf1N1B4_3026 0.64  -0.1 3,403,979 -  Pf1N1B4_3026 0.64  +0.1 3,403,979 -  Pf1N1B4_3026 0.64  +0.8 3,403,982 -  Pf1N1B4_3026 0.65  -2.0 3,403,982 -  Pf1N1B4_3026 0.65  -1.8 3,404,140 +  Pf1N1B4_3026 0.81  -1.2 3,404,140 +  Pf1N1B4_3026 0.81  -3.0 3,404,140 +  Pf1N1B4_3026 0.81  -0.2 3,404,140 +  Pf1N1B4_3026 0.81  +0.4 3,404,141 -  +0.2 3,404,179 +  Pf1N1B4_3026 0.86  -1.8 3,404,179 +  Pf1N1B4_3026 0.86  -0.2 3,404,227 +  -1.4 3,404,227 +  +1.3 3,404,227 +  -0.6 3,404,228 -  +0.8 3,404,228 -  -0.2 3,404,245 +  +1.4 3,404,687 +  Pf1N1B4_3027 0.33  -1.3 3,404,687 +  Pf1N1B4_3027 0.33  +3.4 3,404,687 +  Pf1N1B4_3027 0.33  -1.8 3,404,687 +  Pf1N1B4_3027 0.33  -1.5 3,404,688 -  Pf1N1B4_3027 0.33  +1.2 3,404,964 +  Pf1N1B4_3027 0.58  -0.4 3,405,076 +  Pf1N1B4_3027 0.68  -0.2 3,405,076 +  Pf1N1B4_3027 0.68  -2.7 3,405,076 +  Pf1N1B4_3027 0.68  -0.9 3,405,077 -  Pf1N1B4_3027 0.68  -1.2 3,405,077 -  Pf1N1B4_3027 0.68  -0.8 3,405,117 +  Pf1N1B4_3027 0.71  +0.2 3,405,117 +  +0.8 3,405,118 -  Pf1N1B4_3027 0.72  -0.1 3,405,118 -  Pf1N1B4_3027 0.72  +0.6 3,405,118 -  Pf1N1B4_3027 0.72  +1.2 3,405,118 -  Pf1N1B4_3027 0.72  -0.2 3,405,168 +  Pf1N1B4_3027 0.76  -1.5 
Or see this region's nucleotide sequence