Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3022 and Pf1N1B4_3023 are separated by 411 nucleotides Pf1N1B4_3023 and Pf1N1B4_3024 are separated by 133 nucleotides Pf1N1B4_3024 and Pf1N1B4_3025 are separated by 175 nucleotides Pf1N1B4_3025 and Pf1N1B4_3026 overlap by 11 nucleotides
Pf1N1B4_3022 - Glucans biosynthesis protein D precursor, at 3,398,598 to 3,400,223
_3022
Pf1N1B4_3023 - hypothetical protein, at 3,400,635 to 3,400,820
_3023
Pf1N1B4_3024 - 4-aminobutyraldehyde dehydrogenase (EC 1.2.1.19), at 3,400,954 to 3,402,378
_3024
Pf1N1B4_3025 - Spermidine Putrescine ABC transporter permease component potC (TC_3.A.1.11.1), at 3,402,554 to 3,403,378
_3025
Pf1N1B4_3026 - Spermidine Putrescine ABC transporter permease component PotB (TC 3.A.1.11.1), at 3,403,368 to 3,404,315
_3026
Position (kb)
3400
3401
3402
3403 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3400.152 kb on + strand at 3400.152 kb on + strand at 3400.152 kb on + strand at 3400.177 kb on + strand at 3400.177 kb on + strand at 3400.178 kb on - strand at 3400.178 kb on - strand at 3400.222 kb on + strand at 3400.223 kb on + strand at 3400.223 kb on + strand at 3400.223 kb on + strand at 3400.223 kb on + strand at 3400.223 kb on + strand at 3400.223 kb on + strand at 3400.223 kb on + strand at 3400.223 kb on + strand at 3400.223 kb on + strand at 3400.223 kb on + strand at 3400.223 kb on + strand at 3400.223 kb on + strand at 3400.224 kb on - strand at 3400.224 kb on - strand at 3400.224 kb on - strand at 3400.224 kb on - strand at 3400.224 kb on - strand at 3400.224 kb on - strand at 3400.224 kb on - strand at 3400.224 kb on - strand at 3400.224 kb on - strand at 3400.224 kb on - strand at 3400.227 kb on + strand at 3400.227 kb on + strand at 3400.227 kb on + strand at 3400.227 kb on + strand at 3400.227 kb on + strand at 3400.227 kb on + strand at 3400.228 kb on - strand at 3400.228 kb on - strand at 3400.228 kb on - strand at 3400.320 kb on + strand at 3400.320 kb on + strand at 3400.320 kb on + strand at 3400.320 kb on + strand at 3400.321 kb on - strand at 3400.321 kb on - strand at 3400.321 kb on - strand at 3400.335 kb on + strand at 3400.336 kb on - strand at 3400.336 kb on - strand at 3400.336 kb on - strand at 3400.336 kb on - strand at 3400.341 kb on + strand at 3400.341 kb on + strand at 3400.342 kb on - strand at 3400.342 kb on - strand at 3400.342 kb on - strand at 3400.442 kb on + strand at 3400.443 kb on - strand at 3400.451 kb on + strand at 3400.451 kb on + strand at 3400.451 kb on + strand at 3400.451 kb on + strand at 3400.452 kb on - strand at 3400.468 kb on + strand at 3400.472 kb on + strand at 3400.472 kb on + strand at 3400.472 kb on + strand at 3400.509 kb on + strand at 3400.510 kb on - strand at 3400.510 kb on - strand at 3400.510 kb on - strand at 3400.510 kb on - strand at 3400.514 kb on + strand at 3400.584 kb on + strand at 3400.617 kb on + strand at 3400.617 kb on + strand at 3400.617 kb on + strand at 3400.617 kb on + strand at 3400.617 kb on + strand at 3400.617 kb on + strand at 3400.617 kb on + strand at 3400.617 kb on + strand at 3400.617 kb on + strand at 3400.617 kb on + strand at 3400.618 kb on - strand at 3400.618 kb on - strand at 3400.618 kb on - strand at 3400.618 kb on - strand at 3400.618 kb on - strand at 3400.618 kb on - strand at 3400.619 kb on + strand at 3400.619 kb on + strand at 3400.619 kb on + strand at 3400.619 kb on + strand at 3400.619 kb on + strand at 3400.619 kb on + strand at 3400.619 kb on + strand at 3400.619 kb on + strand at 3400.619 kb on + strand at 3400.619 kb on + strand at 3400.619 kb on + strand at 3400.619 kb on + strand at 3400.619 kb on + strand at 3400.619 kb on + strand at 3400.619 kb on + strand at 3400.620 kb on - strand at 3400.620 kb on - strand at 3400.620 kb on - strand at 3400.645 kb on + strand at 3400.645 kb on + strand at 3400.645 kb on + strand at 3400.645 kb on + strand at 3400.646 kb on - strand at 3400.646 kb on - strand at 3400.666 kb on + strand, within Pf1N1B4_3023 at 3400.667 kb on - strand, within Pf1N1B4_3023 at 3400.667 kb on - strand, within Pf1N1B4_3023 at 3400.667 kb on - strand, within Pf1N1B4_3023 at 3400.832 kb on + strand at 3400.955 kb on + strand at 3400.955 kb on + strand at 3400.955 kb on + strand at 3400.956 kb on - strand at 3400.956 kb on - strand at 3400.956 kb on - strand at 3400.988 kb on + strand at 3400.988 kb on + strand at 3400.988 kb on + strand at 3400.989 kb on - strand at 3400.989 kb on - strand at 3401.003 kb on + strand at 3401.003 kb on + strand at 3401.003 kb on + strand at 3401.004 kb on - strand at 3401.168 kb on + strand, within Pf1N1B4_3024 at 3401.169 kb on - strand, within Pf1N1B4_3024 at 3401.453 kb on + strand, within Pf1N1B4_3024 at 3401.454 kb on - strand, within Pf1N1B4_3024 at 3401.461 kb on - strand, within Pf1N1B4_3024 at 3401.501 kb on + strand, within Pf1N1B4_3024 at 3401.501 kb on + strand, within Pf1N1B4_3024 at 3401.502 kb on - strand, within Pf1N1B4_3024 at 3401.502 kb on - strand, within Pf1N1B4_3024 at 3401.502 kb on - strand at 3401.502 kb on - strand, within Pf1N1B4_3024 at 3401.502 kb on - strand, within Pf1N1B4_3024 at 3401.502 kb on - strand, within Pf1N1B4_3024 at 3401.519 kb on + strand at 3401.520 kb on - strand, within Pf1N1B4_3024 at 3401.520 kb on - strand, within Pf1N1B4_3024 at 3401.558 kb on + strand, within Pf1N1B4_3024 at 3401.558 kb on + strand, within Pf1N1B4_3024 at 3401.558 kb on + strand, within Pf1N1B4_3024 at 3401.559 kb on - strand, within Pf1N1B4_3024 at 3401.559 kb on - strand, within Pf1N1B4_3024 at 3401.559 kb on - strand, within Pf1N1B4_3024 at 3401.577 kb on + strand, within Pf1N1B4_3024 at 3401.577 kb on + strand, within Pf1N1B4_3024 at 3401.577 kb on + strand, within Pf1N1B4_3024 at 3401.578 kb on - strand, within Pf1N1B4_3024 at 3401.578 kb on - strand, within Pf1N1B4_3024 at 3401.584 kb on + strand, within Pf1N1B4_3024 at 3401.585 kb on - strand, within Pf1N1B4_3024 at 3401.728 kb on + strand, within Pf1N1B4_3024 at 3401.729 kb on - strand, within Pf1N1B4_3024 at 3401.778 kb on - strand, within Pf1N1B4_3024 at 3401.787 kb on + strand, within Pf1N1B4_3024 at 3401.787 kb on + strand at 3401.787 kb on + strand, within Pf1N1B4_3024 at 3401.787 kb on + strand, within Pf1N1B4_3024 at 3401.787 kb on + strand, within Pf1N1B4_3024 at 3401.788 kb on - strand, within Pf1N1B4_3024 at 3401.788 kb on - strand, within Pf1N1B4_3024 at 3401.788 kb on - strand, within Pf1N1B4_3024 at 3401.788 kb on - strand, within Pf1N1B4_3024 at 3401.791 kb on + strand, within Pf1N1B4_3024 at 3401.791 kb on + strand, within Pf1N1B4_3024 at 3401.791 kb on + strand, within Pf1N1B4_3024 at 3401.791 kb on + strand, within Pf1N1B4_3024 at 3401.791 kb on + strand, within Pf1N1B4_3024 at 3401.792 kb on - strand, within Pf1N1B4_3024 at 3401.792 kb on - strand, within Pf1N1B4_3024 at 3401.792 kb on - strand, within Pf1N1B4_3024 at 3401.792 kb on - strand, within Pf1N1B4_3024 at 3401.877 kb on + strand, within Pf1N1B4_3024 at 3401.877 kb on + strand, within Pf1N1B4_3024 at 3401.877 kb on + strand, within Pf1N1B4_3024 at 3401.878 kb on - strand, within Pf1N1B4_3024 at 3401.880 kb on + strand, within Pf1N1B4_3024 at 3401.930 kb on + strand, within Pf1N1B4_3024 at 3402.002 kb on + strand, within Pf1N1B4_3024 at 3402.002 kb on + strand, within Pf1N1B4_3024 at 3402.003 kb on - strand, within Pf1N1B4_3024 at 3402.003 kb on - strand, within Pf1N1B4_3024 at 3402.101 kb on + strand, within Pf1N1B4_3024 at 3402.101 kb on + strand at 3402.102 kb on - strand, within Pf1N1B4_3024 at 3402.287 kb on - strand at 3402.382 kb on + strand at 3402.382 kb on + strand at 3402.382 kb on + strand at 3402.382 kb on + strand at 3402.382 kb on + strand at 3402.382 kb on + strand at 3402.383 kb on - strand at 3402.383 kb on - strand at 3402.383 kb on - strand at 3402.383 kb on - strand at 3402.383 kb on - strand at 3402.383 kb on - strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.486 kb on + strand at 3402.486 kb on + strand at 3402.487 kb on - strand at 3402.487 kb on - strand at 3402.487 kb on - strand at 3402.487 kb on - strand at 3402.555 kb on + strand at 3402.600 kb on + strand at 3402.600 kb on + strand at 3402.600 kb on + strand at 3402.603 kb on + strand at 3402.603 kb on + strand at 3402.603 kb on + strand at 3402.603 kb on + strand at 3402.604 kb on - strand at 3402.825 kb on + strand, within Pf1N1B4_3025 at 3402.825 kb on + strand, within Pf1N1B4_3025 at 3402.882 kb on + strand, within Pf1N1B4_3025 at 3402.883 kb on - strand, within Pf1N1B4_3025 at 3402.883 kb on - strand, within Pf1N1B4_3025 at 3402.883 kb on - strand, within Pf1N1B4_3025 at 3402.883 kb on - strand at 3402.883 kb on - strand, within Pf1N1B4_3025 at 3402.883 kb on - strand, within Pf1N1B4_3025 at 3402.918 kb on + strand, within Pf1N1B4_3025 at 3402.918 kb on + strand, within Pf1N1B4_3025 at 3402.919 kb on - strand, within Pf1N1B4_3025 at 3403.335 kb on - strand at 3403.366 kb on + strand at 3403.366 kb on + strand at 3403.366 kb on + strand at 3403.367 kb on - strand at 3403.367 kb on - strand at 3403.367 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 3,400,152 + +1.5 3,400,152 + -0.7 3,400,152 + +0.5 3,400,177 + -0.6 3,400,177 + +0.2 3,400,178 - +1.8 3,400,178 - +0.9 3,400,222 + +0.4 3,400,223 + +1.2 3,400,223 + +0.8 3,400,223 + -1.8 3,400,223 + -1.7 3,400,223 + -1.1 3,400,223 + -1.2 3,400,223 + -1.9 3,400,223 + +0.2 3,400,223 + -0.9 3,400,223 + +1.2 3,400,223 + +1.3 3,400,223 + +0.2 3,400,224 - -0.8 3,400,224 - -2.0 3,400,224 - -0.8 3,400,224 - -0.8 3,400,224 - -0.2 3,400,224 - -0.8 3,400,224 - -1.2 3,400,224 - +0.9 3,400,224 - -1.8 3,400,224 - +0.7 3,400,227 + +0.6 3,400,227 + -0.0 3,400,227 + +0.2 3,400,227 + -2.5 3,400,227 + +1.0 3,400,227 + +0.0 3,400,228 - +0.5 3,400,228 - +0.8 3,400,228 - -0.2 3,400,320 + -1.2 3,400,320 + +0.0 3,400,320 + +0.8 3,400,320 + +1.5 3,400,321 - -1.4 3,400,321 - +0.1 3,400,321 - -1.2 3,400,335 + -0.7 3,400,336 - +1.4 3,400,336 - +0.4 3,400,336 - -0.8 3,400,336 - -1.2 3,400,341 + -1.5 3,400,341 + -0.8 3,400,342 - +0.1 3,400,342 - -1.2 3,400,342 - -1.3 3,400,442 + +0.6 3,400,443 - +0.1 3,400,451 + +0.4 3,400,451 + -0.9 3,400,451 + -0.3 3,400,451 + +0.5 3,400,452 - -0.1 3,400,468 + +1.8 3,400,472 + +0.5 3,400,472 + +0.2 3,400,472 + +1.4 3,400,509 + +0.6 3,400,510 - -0.2 3,400,510 - -1.2 3,400,510 - +0.8 3,400,510 - +1.2 3,400,514 + -1.7 3,400,584 + +0.4 3,400,617 + +0.8 3,400,617 + -0.0 3,400,617 + -0.8 3,400,617 + -1.2 3,400,617 + -2.0 3,400,617 + -0.5 3,400,617 + +0.4 3,400,617 + -0.6 3,400,617 + -1.5 3,400,617 + +0.4 3,400,618 - -0.2 3,400,618 - +0.1 3,400,618 - -1.2 3,400,618 - +0.4 3,400,618 - -0.7 3,400,618 - +0.1 3,400,619 + -1.4 3,400,619 + +0.3 3,400,619 + -0.2 3,400,619 + +0.8 3,400,619 + -0.8 3,400,619 + +0.3 3,400,619 + -0.2 3,400,619 + +0.4 3,400,619 + -0.9 3,400,619 + +0.5 3,400,619 + -0.1 3,400,619 + -1.5 3,400,619 + -2.9 3,400,619 + -0.1 3,400,619 + +0.4 3,400,620 - +1.1 3,400,620 - +0.2 3,400,620 - -1.2 3,400,645 + +0.8 3,400,645 + -0.6 3,400,645 + +0.1 3,400,645 + -0.6 3,400,646 - -0.8 3,400,646 - +0.5 3,400,666 + Pf1N1B4_3023 0.17 -2.0 3,400,667 - Pf1N1B4_3023 0.17 +0.5 3,400,667 - Pf1N1B4_3023 0.17 -2.8 3,400,667 - Pf1N1B4_3023 0.17 +0.4 3,400,832 + -1.0 3,400,955 + +1.8 3,400,955 + +1.3 3,400,955 + +1.6 3,400,956 - +0.0 3,400,956 - +1.2 3,400,956 - +0.0 3,400,988 + -3.3 3,400,988 + +0.8 3,400,988 + -0.8 3,400,989 - -1.0 3,400,989 - -1.8 3,401,003 + +0.4 3,401,003 + -1.1 3,401,003 + -0.3 3,401,004 - +1.4 3,401,168 + Pf1N1B4_3024 0.15 -0.0 3,401,169 - Pf1N1B4_3024 0.15 -2.2 3,401,453 + Pf1N1B4_3024 0.35 +0.2 3,401,454 - Pf1N1B4_3024 0.35 -0.3 3,401,461 - Pf1N1B4_3024 0.36 +0.0 3,401,501 + Pf1N1B4_3024 0.38 -0.5 3,401,501 + Pf1N1B4_3024 0.38 +0.5 3,401,502 - Pf1N1B4_3024 0.38 -1.2 3,401,502 - Pf1N1B4_3024 0.38 -0.5 3,401,502 - -2.0 3,401,502 - Pf1N1B4_3024 0.38 +0.8 3,401,502 - Pf1N1B4_3024 0.38 +0.4 3,401,502 - Pf1N1B4_3024 0.38 +0.8 3,401,519 + -0.8 3,401,520 - Pf1N1B4_3024 0.40 -0.6 3,401,520 - Pf1N1B4_3024 0.40 -1.8 3,401,558 + Pf1N1B4_3024 0.42 -0.4 3,401,558 + Pf1N1B4_3024 0.42 -0.2 3,401,558 + Pf1N1B4_3024 0.42 +1.0 3,401,559 - Pf1N1B4_3024 0.42 +1.0 3,401,559 - Pf1N1B4_3024 0.42 +0.1 3,401,559 - Pf1N1B4_3024 0.42 +0.8 3,401,577 + Pf1N1B4_3024 0.44 -0.0 3,401,577 + Pf1N1B4_3024 0.44 +1.8 3,401,577 + Pf1N1B4_3024 0.44 +0.5 3,401,578 - Pf1N1B4_3024 0.44 -1.4 3,401,578 - Pf1N1B4_3024 0.44 -0.8 3,401,584 + Pf1N1B4_3024 0.44 -0.0 3,401,585 - Pf1N1B4_3024 0.44 +1.3 3,401,728 + Pf1N1B4_3024 0.54 +0.2 3,401,729 - Pf1N1B4_3024 0.54 -0.8 3,401,778 - Pf1N1B4_3024 0.58 -0.3 3,401,787 + Pf1N1B4_3024 0.58 +0.1 3,401,787 + +1.2 3,401,787 + Pf1N1B4_3024 0.58 +0.8 3,401,787 + Pf1N1B4_3024 0.58 +0.6 3,401,787 + Pf1N1B4_3024 0.58 -0.8 3,401,788 - Pf1N1B4_3024 0.59 -0.5 3,401,788 - Pf1N1B4_3024 0.59 -1.3 3,401,788 - Pf1N1B4_3024 0.59 +0.2 3,401,788 - Pf1N1B4_3024 0.59 +0.2 3,401,791 + Pf1N1B4_3024 0.59 +0.8 3,401,791 + Pf1N1B4_3024 0.59 -0.4 3,401,791 + Pf1N1B4_3024 0.59 -0.9 3,401,791 + Pf1N1B4_3024 0.59 -0.3 3,401,791 + Pf1N1B4_3024 0.59 +2.4 3,401,792 - Pf1N1B4_3024 0.59 +1.2 3,401,792 - Pf1N1B4_3024 0.59 +0.4 3,401,792 - Pf1N1B4_3024 0.59 +1.2 3,401,792 - Pf1N1B4_3024 0.59 -1.2 3,401,877 + Pf1N1B4_3024 0.65 +2.4 3,401,877 + Pf1N1B4_3024 0.65 +0.3 3,401,877 + Pf1N1B4_3024 0.65 -3.8 3,401,878 - Pf1N1B4_3024 0.65 +0.2 3,401,880 + Pf1N1B4_3024 0.65 +0.0 3,401,930 + Pf1N1B4_3024 0.68 -0.5 3,402,002 + Pf1N1B4_3024 0.74 -0.7 3,402,002 + Pf1N1B4_3024 0.74 -0.0 3,402,003 - Pf1N1B4_3024 0.74 +1.1 3,402,003 - Pf1N1B4_3024 0.74 -1.4 3,402,101 + Pf1N1B4_3024 0.80 -0.2 3,402,101 + +0.2 3,402,102 - Pf1N1B4_3024 0.81 +0.2 3,402,287 - -1.1 3,402,382 + -1.0 3,402,382 + -0.8 3,402,382 + -0.1 3,402,382 + -0.5 3,402,382 + -0.4 3,402,382 + -0.5 3,402,383 - +0.3 3,402,383 - +1.1 3,402,383 - +0.8 3,402,383 - +0.2 3,402,383 - +1.8 3,402,383 - +1.5 3,402,403 + -2.8 3,402,403 + +1.8 3,402,403 + +0.1 3,402,403 + -0.2 3,402,403 + -2.2 3,402,403 + -0.2 3,402,403 + +1.8 3,402,403 + -0.4 3,402,403 + -0.7 3,402,403 + -0.2 3,402,403 + -0.8 3,402,403 + +0.1 3,402,403 + -0.8 3,402,403 + -1.0 3,402,403 + +1.8 3,402,403 + +0.8 3,402,403 + +0.5 3,402,404 - -1.5 3,402,404 - -0.8 3,402,404 - -0.8 3,402,404 - +0.1 3,402,404 - -1.5 3,402,404 - +0.1 3,402,404 - -0.3 3,402,404 - -0.4 3,402,404 - -0.0 3,402,404 - +0.7 3,402,404 - -2.0 3,402,404 - -0.8 3,402,404 - -0.8 3,402,404 - +1.4 3,402,404 - -0.4 3,402,486 + +0.7 3,402,486 + +0.2 3,402,487 - +1.4 3,402,487 - -0.2 3,402,487 - -2.0 3,402,487 - -2.4 3,402,555 + +0.5 3,402,600 + -1.4 3,402,600 + -0.4 3,402,600 + +0.1 3,402,603 + -0.2 3,402,603 + -0.4 3,402,603 + +0.8 3,402,603 + -0.2 3,402,604 - +0.8 3,402,825 + Pf1N1B4_3025 0.33 +1.6 3,402,825 + Pf1N1B4_3025 0.33 -0.9 3,402,882 + Pf1N1B4_3025 0.40 -1.9 3,402,883 - Pf1N1B4_3025 0.40 +0.6 3,402,883 - Pf1N1B4_3025 0.40 +0.2 3,402,883 - Pf1N1B4_3025 0.40 -1.0 3,402,883 - +0.8 3,402,883 - Pf1N1B4_3025 0.40 -2.5 3,402,883 - Pf1N1B4_3025 0.40 -0.2 3,402,918 + Pf1N1B4_3025 0.44 +0.4 3,402,918 + Pf1N1B4_3025 0.44 +0.1 3,402,919 - Pf1N1B4_3025 0.44 -1.2 3,403,335 - +1.8 3,403,366 + -0.0 3,403,366 + +0.2 3,403,366 + +1.4 3,403,367 - +0.8 3,403,367 - -2.0 3,403,367 - +0.8
Or see this region's nucleotide sequence