Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2880 and Pf1N1B4_2881 are separated by 146 nucleotides Pf1N1B4_2881 and Pf1N1B4_2882 are separated by 63 nucleotides
Pf1N1B4_2880 - Ketol-acid reductoisomerase (EC 1.1.1.86), at 3,226,732 to 3,227,748
_2880
Pf1N1B4_2881 - CDP-diacylglycerol--serine O-phosphatidyltransferase (EC 2.7.8.8), at 3,227,895 to 3,228,752
_2881
Pf1N1B4_2882 - Putative sulfite oxidase subunit YedY, at 3,228,816 to 3,229,829
_2882
Position (kb)
3227
3228
3229 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3226.996 kb on + strand, within Pf1N1B4_2880 at 3226.996 kb on + strand, within Pf1N1B4_2880 at 3226.996 kb on + strand, within Pf1N1B4_2880 at 3226.996 kb on + strand, within Pf1N1B4_2880 at 3226.997 kb on - strand, within Pf1N1B4_2880 at 3226.997 kb on - strand, within Pf1N1B4_2880 at 3226.997 kb on - strand, within Pf1N1B4_2880 at 3227.068 kb on + strand, within Pf1N1B4_2880 at 3227.068 kb on + strand, within Pf1N1B4_2880 at 3227.068 kb on + strand, within Pf1N1B4_2880 at 3227.069 kb on - strand at 3227.069 kb on - strand at 3227.185 kb on - strand, within Pf1N1B4_2880 at 3227.188 kb on + strand, within Pf1N1B4_2880 at 3227.188 kb on + strand, within Pf1N1B4_2880 at 3227.188 kb on + strand, within Pf1N1B4_2880 at 3227.188 kb on + strand, within Pf1N1B4_2880 at 3227.188 kb on + strand, within Pf1N1B4_2880 at 3227.188 kb on + strand, within Pf1N1B4_2880 at 3227.188 kb on + strand at 3227.189 kb on - strand, within Pf1N1B4_2880 at 3227.189 kb on - strand, within Pf1N1B4_2880 at 3227.230 kb on + strand, within Pf1N1B4_2880 at 3227.230 kb on + strand, within Pf1N1B4_2880 at 3227.231 kb on - strand, within Pf1N1B4_2880 at 3227.348 kb on + strand, within Pf1N1B4_2880 at 3227.401 kb on + strand, within Pf1N1B4_2880 at 3227.401 kb on + strand, within Pf1N1B4_2880 at 3227.401 kb on + strand, within Pf1N1B4_2880 at 3227.401 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.447 kb on - strand, within Pf1N1B4_2880 at 3227.447 kb on - strand, within Pf1N1B4_2880 at 3227.447 kb on - strand, within Pf1N1B4_2880 at 3227.447 kb on - strand, within Pf1N1B4_2880 at 3227.447 kb on - strand, within Pf1N1B4_2880 at 3227.447 kb on - strand, within Pf1N1B4_2880 at 3227.473 kb on + strand, within Pf1N1B4_2880 at 3227.473 kb on + strand, within Pf1N1B4_2880 at 3227.473 kb on + strand, within Pf1N1B4_2880 at 3227.473 kb on + strand, within Pf1N1B4_2880 at 3227.473 kb on + strand, within Pf1N1B4_2880 at 3227.473 kb on + strand, within Pf1N1B4_2880 at 3227.506 kb on + strand, within Pf1N1B4_2880 at 3227.506 kb on + strand, within Pf1N1B4_2880 at 3227.506 kb on + strand, within Pf1N1B4_2880 at 3227.506 kb on + strand, within Pf1N1B4_2880 at 3227.506 kb on + strand, within Pf1N1B4_2880 at 3227.506 kb on + strand, within Pf1N1B4_2880 at 3227.507 kb on - strand, within Pf1N1B4_2880 at 3227.571 kb on + strand, within Pf1N1B4_2880 at 3227.571 kb on + strand, within Pf1N1B4_2880 at 3227.571 kb on + strand, within Pf1N1B4_2880 at 3227.571 kb on + strand, within Pf1N1B4_2880 at 3227.571 kb on + strand, within Pf1N1B4_2880 at 3227.571 kb on + strand, within Pf1N1B4_2880 at 3227.571 kb on + strand, within Pf1N1B4_2880 at 3227.571 kb on + strand, within Pf1N1B4_2880 at 3227.571 kb on + strand, within Pf1N1B4_2880 at 3227.571 kb on + strand, within Pf1N1B4_2880 at 3227.572 kb on - strand, within Pf1N1B4_2880 at 3227.572 kb on - strand, within Pf1N1B4_2880 at 3227.572 kb on - strand, within Pf1N1B4_2880 at 3227.587 kb on + strand, within Pf1N1B4_2880 at 3227.649 kb on + strand at 3227.746 kb on + strand at 3227.746 kb on + strand at 3227.746 kb on + strand at 3227.746 kb on + strand at 3227.746 kb on + strand at 3227.746 kb on + strand at 3227.746 kb on + strand at 3227.746 kb on + strand at 3227.746 kb on + strand at 3227.746 kb on + strand at 3227.746 kb on + strand at 3227.746 kb on + strand at 3227.777 kb on + strand at 3227.777 kb on + strand at 3227.777 kb on + strand at 3227.814 kb on + strand at 3227.814 kb on + strand at 3227.816 kb on + strand at 3228.898 kb on + strand at 3228.898 kb on + strand at 3228.901 kb on + strand at 3228.901 kb on + strand at 3228.901 kb on + strand at 3228.901 kb on + strand at 3229.104 kb on + strand, within Pf1N1B4_2882 at 3229.104 kb on + strand, within Pf1N1B4_2882 at 3229.105 kb on - strand, within Pf1N1B4_2882 at 3229.253 kb on + strand at 3229.253 kb on + strand, within Pf1N1B4_2882 at 3229.257 kb on + strand, within Pf1N1B4_2882 at 3229.257 kb on + strand, within Pf1N1B4_2882 at 3229.395 kb on - strand, within Pf1N1B4_2882 at 3229.437 kb on + strand, within Pf1N1B4_2882 at 3229.437 kb on + strand, within Pf1N1B4_2882 at 3229.438 kb on - strand, within Pf1N1B4_2882 at 3229.441 kb on + strand, within Pf1N1B4_2882 at 3229.441 kb on + strand, within Pf1N1B4_2882 at 3229.441 kb on + strand, within Pf1N1B4_2882 at 3229.441 kb on + strand, within Pf1N1B4_2882 at 3229.442 kb on - strand at 3229.442 kb on - strand, within Pf1N1B4_2882 at 3229.500 kb on + strand, within Pf1N1B4_2882 at 3229.500 kb on + strand, within Pf1N1B4_2882 at 3229.500 kb on + strand, within Pf1N1B4_2882 at 3229.500 kb on + strand, within Pf1N1B4_2882 at 3229.500 kb on + strand, within Pf1N1B4_2882 at 3229.500 kb on + strand, within Pf1N1B4_2882 at 3229.500 kb on + strand, within Pf1N1B4_2882 at 3229.500 kb on + strand, within Pf1N1B4_2882 at 3229.501 kb on - strand, within Pf1N1B4_2882 at 3229.501 kb on - strand, within Pf1N1B4_2882 at 3229.560 kb on + strand, within Pf1N1B4_2882 at 3229.560 kb on + strand, within Pf1N1B4_2882 at 3229.622 kb on + strand, within Pf1N1B4_2882 at 3229.622 kb on + strand, within Pf1N1B4_2882 at 3229.623 kb on - strand, within Pf1N1B4_2882 at 3229.660 kb on - strand, within Pf1N1B4_2882
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 3,226,996 + Pf1N1B4_2880 0.26 +0.5 3,226,996 + Pf1N1B4_2880 0.26 +1.5 3,226,996 + Pf1N1B4_2880 0.26 +0.7 3,226,996 + Pf1N1B4_2880 0.26 -0.2 3,226,997 - Pf1N1B4_2880 0.26 -1.2 3,226,997 - Pf1N1B4_2880 0.26 -1.1 3,226,997 - Pf1N1B4_2880 0.26 +0.9 3,227,068 + Pf1N1B4_2880 0.33 -1.5 3,227,068 + Pf1N1B4_2880 0.33 +0.5 3,227,068 + Pf1N1B4_2880 0.33 -1.0 3,227,069 - +0.9 3,227,069 - -0.1 3,227,185 - Pf1N1B4_2880 0.45 +1.8 3,227,188 + Pf1N1B4_2880 0.45 +2.8 3,227,188 + Pf1N1B4_2880 0.45 -1.1 3,227,188 + Pf1N1B4_2880 0.45 -0.3 3,227,188 + Pf1N1B4_2880 0.45 +1.4 3,227,188 + Pf1N1B4_2880 0.45 -0.6 3,227,188 + Pf1N1B4_2880 0.45 +1.3 3,227,188 + +0.9 3,227,189 - Pf1N1B4_2880 0.45 +1.1 3,227,189 - Pf1N1B4_2880 0.45 -0.3 3,227,230 + Pf1N1B4_2880 0.49 -0.2 3,227,230 + Pf1N1B4_2880 0.49 +0.1 3,227,231 - Pf1N1B4_2880 0.49 +0.3 3,227,348 + Pf1N1B4_2880 0.61 -2.4 3,227,401 + Pf1N1B4_2880 0.66 +0.9 3,227,401 + Pf1N1B4_2880 0.66 +1.7 3,227,401 + Pf1N1B4_2880 0.66 -0.3 3,227,401 + Pf1N1B4_2880 0.66 -0.2 3,227,446 + Pf1N1B4_2880 0.70 -0.1 3,227,446 + Pf1N1B4_2880 0.70 +0.9 3,227,446 + Pf1N1B4_2880 0.70 +1.7 3,227,446 + Pf1N1B4_2880 0.70 +0.2 3,227,446 + Pf1N1B4_2880 0.70 -0.7 3,227,446 + Pf1N1B4_2880 0.70 -1.2 3,227,446 + Pf1N1B4_2880 0.70 +0.1 3,227,446 + Pf1N1B4_2880 0.70 +0.9 3,227,446 + Pf1N1B4_2880 0.70 +0.1 3,227,446 + Pf1N1B4_2880 0.70 -1.2 3,227,446 + Pf1N1B4_2880 0.70 -0.9 3,227,446 + Pf1N1B4_2880 0.70 +0.1 3,227,446 + Pf1N1B4_2880 0.70 -0.7 3,227,446 + Pf1N1B4_2880 0.70 +1.0 3,227,446 + Pf1N1B4_2880 0.70 -1.0 3,227,446 + Pf1N1B4_2880 0.70 +0.3 3,227,446 + Pf1N1B4_2880 0.70 +0.9 3,227,446 + Pf1N1B4_2880 0.70 +0.3 3,227,446 + Pf1N1B4_2880 0.70 -0.7 3,227,447 - Pf1N1B4_2880 0.70 +0.9 3,227,447 - Pf1N1B4_2880 0.70 -0.1 3,227,447 - Pf1N1B4_2880 0.70 -0.3 3,227,447 - Pf1N1B4_2880 0.70 -1.8 3,227,447 - Pf1N1B4_2880 0.70 -1.5 3,227,447 - Pf1N1B4_2880 0.70 +0.1 3,227,473 + Pf1N1B4_2880 0.73 -0.8 3,227,473 + Pf1N1B4_2880 0.73 +0.1 3,227,473 + Pf1N1B4_2880 0.73 +0.2 3,227,473 + Pf1N1B4_2880 0.73 +0.1 3,227,473 + Pf1N1B4_2880 0.73 -0.5 3,227,473 + Pf1N1B4_2880 0.73 +0.9 3,227,506 + Pf1N1B4_2880 0.76 +1.0 3,227,506 + Pf1N1B4_2880 0.76 -0.1 3,227,506 + Pf1N1B4_2880 0.76 +0.0 3,227,506 + Pf1N1B4_2880 0.76 +1.5 3,227,506 + Pf1N1B4_2880 0.76 -0.9 3,227,506 + Pf1N1B4_2880 0.76 +0.9 3,227,507 - Pf1N1B4_2880 0.76 +1.6 3,227,571 + Pf1N1B4_2880 0.82 -0.1 3,227,571 + Pf1N1B4_2880 0.82 +0.1 3,227,571 + Pf1N1B4_2880 0.82 +0.6 3,227,571 + Pf1N1B4_2880 0.82 -0.3 3,227,571 + Pf1N1B4_2880 0.82 +0.5 3,227,571 + Pf1N1B4_2880 0.82 +0.7 3,227,571 + Pf1N1B4_2880 0.82 +0.7 3,227,571 + Pf1N1B4_2880 0.82 -0.1 3,227,571 + Pf1N1B4_2880 0.82 +0.4 3,227,571 + Pf1N1B4_2880 0.82 +1.6 3,227,572 - Pf1N1B4_2880 0.83 +0.9 3,227,572 - Pf1N1B4_2880 0.83 +0.0 3,227,572 - Pf1N1B4_2880 0.83 -2.6 3,227,587 + Pf1N1B4_2880 0.84 -0.1 3,227,649 + -0.6 3,227,746 + -1.7 3,227,746 + +0.2 3,227,746 + -0.1 3,227,746 + -0.1 3,227,746 + -2.9 3,227,746 + +2.1 3,227,746 + +0.6 3,227,746 + +0.5 3,227,746 + +0.3 3,227,746 + -1.9 3,227,746 + +1.9 3,227,746 + +0.7 3,227,777 + +1.4 3,227,777 + -0.7 3,227,777 + +0.4 3,227,814 + +1.9 3,227,814 + +0.5 3,227,816 + +0.9 3,228,898 + +0.9 3,228,898 + +1.3 3,228,901 + -0.3 3,228,901 + -0.3 3,228,901 + -0.9 3,228,901 + -1.9 3,229,104 + Pf1N1B4_2882 0.28 +0.5 3,229,104 + Pf1N1B4_2882 0.28 -0.2 3,229,105 - Pf1N1B4_2882 0.29 -0.7 3,229,253 + +0.9 3,229,253 + Pf1N1B4_2882 0.43 +0.9 3,229,257 + Pf1N1B4_2882 0.43 +0.5 3,229,257 + Pf1N1B4_2882 0.43 -0.6 3,229,395 - Pf1N1B4_2882 0.57 +0.9 3,229,437 + Pf1N1B4_2882 0.61 -1.9 3,229,437 + Pf1N1B4_2882 0.61 -1.1 3,229,438 - Pf1N1B4_2882 0.61 -0.6 3,229,441 + Pf1N1B4_2882 0.62 -0.9 3,229,441 + Pf1N1B4_2882 0.62 +0.4 3,229,441 + Pf1N1B4_2882 0.62 -0.7 3,229,441 + Pf1N1B4_2882 0.62 -0.7 3,229,442 - -0.1 3,229,442 - Pf1N1B4_2882 0.62 +1.3 3,229,500 + Pf1N1B4_2882 0.67 -2.3 3,229,500 + Pf1N1B4_2882 0.67 -1.4 3,229,500 + Pf1N1B4_2882 0.67 -1.4 3,229,500 + Pf1N1B4_2882 0.67 +0.2 3,229,500 + Pf1N1B4_2882 0.67 +0.2 3,229,500 + Pf1N1B4_2882 0.67 -0.9 3,229,500 + Pf1N1B4_2882 0.67 -0.3 3,229,500 + Pf1N1B4_2882 0.67 -0.4 3,229,501 - Pf1N1B4_2882 0.68 -0.1 3,229,501 - Pf1N1B4_2882 0.68 -0.6 3,229,560 + Pf1N1B4_2882 0.73 +0.9 3,229,560 + Pf1N1B4_2882 0.73 -1.0 3,229,622 + Pf1N1B4_2882 0.79 -1.6 3,229,622 + Pf1N1B4_2882 0.79 -0.7 3,229,623 - Pf1N1B4_2882 0.80 +1.1 3,229,660 - Pf1N1B4_2882 0.83 -1.1
Or see this region's nucleotide sequence