Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2538 and Pf1N1B4_2539 are separated by 49 nucleotides Pf1N1B4_2539 and Pf1N1B4_2540 are separated by 198 nucleotides
Pf1N1B4_2538 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 2,879,841 to 2,880,965
_2538
Pf1N1B4_2539 - ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1), at 2,881,015 to 2,882,058
_2539
Pf1N1B4_2540 - Spermidine Putrescine ABC transporter permease component PotB (TC 3.A.1.11.1), at 2,882,257 to 2,883,504
_2540
Position (kb)
2881
2882
2883 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2880.126 kb on + strand, within Pf1N1B4_2538 at 2880.127 kb on - strand, within Pf1N1B4_2538 at 2880.127 kb on - strand, within Pf1N1B4_2538 at 2880.127 kb on - strand, within Pf1N1B4_2538 at 2880.127 kb on - strand, within Pf1N1B4_2538 at 2880.127 kb on - strand, within Pf1N1B4_2538 at 2880.441 kb on + strand, within Pf1N1B4_2538 at 2880.441 kb on + strand, within Pf1N1B4_2538 at 2880.441 kb on + strand, within Pf1N1B4_2538 at 2880.442 kb on - strand at 2880.540 kb on + strand, within Pf1N1B4_2538 at 2880.540 kb on + strand, within Pf1N1B4_2538 at 2880.541 kb on - strand, within Pf1N1B4_2538 at 2880.795 kb on + strand, within Pf1N1B4_2538 at 2880.795 kb on + strand, within Pf1N1B4_2538 at 2880.795 kb on + strand, within Pf1N1B4_2538 at 2880.795 kb on + strand, within Pf1N1B4_2538 at 2880.795 kb on + strand, within Pf1N1B4_2538 at 2880.795 kb on + strand, within Pf1N1B4_2538 at 2880.795 kb on + strand, within Pf1N1B4_2538 at 2880.796 kb on - strand at 2880.952 kb on + strand at 2881.012 kb on + strand at 2881.012 kb on + strand at 2881.012 kb on + strand at 2881.012 kb on + strand at 2881.012 kb on + strand at 2881.012 kb on + strand at 2881.012 kb on + strand at 2881.012 kb on + strand at 2881.012 kb on + strand at 2881.013 kb on - strand at 2881.013 kb on - strand at 2881.013 kb on - strand at 2881.013 kb on - strand at 2881.013 kb on - strand at 2881.013 kb on - strand at 2881.013 kb on - strand at 2881.147 kb on + strand, within Pf1N1B4_2539 at 2881.148 kb on - strand, within Pf1N1B4_2539 at 2881.148 kb on - strand, within Pf1N1B4_2539 at 2881.195 kb on + strand, within Pf1N1B4_2539 at 2881.195 kb on + strand, within Pf1N1B4_2539 at 2881.195 kb on + strand, within Pf1N1B4_2539 at 2881.259 kb on + strand, within Pf1N1B4_2539 at 2881.260 kb on - strand at 2881.348 kb on + strand, within Pf1N1B4_2539 at 2881.349 kb on - strand, within Pf1N1B4_2539 at 2881.510 kb on + strand, within Pf1N1B4_2539 at 2881.510 kb on + strand, within Pf1N1B4_2539 at 2881.510 kb on + strand, within Pf1N1B4_2539 at 2881.511 kb on - strand, within Pf1N1B4_2539 at 2881.561 kb on + strand, within Pf1N1B4_2539 at 2881.561 kb on + strand, within Pf1N1B4_2539 at 2881.568 kb on + strand, within Pf1N1B4_2539 at 2881.568 kb on + strand, within Pf1N1B4_2539 at 2881.568 kb on + strand, within Pf1N1B4_2539 at 2881.666 kb on + strand, within Pf1N1B4_2539 at 2881.702 kb on + strand, within Pf1N1B4_2539 at 2881.768 kb on + strand, within Pf1N1B4_2539 at 2881.769 kb on - strand, within Pf1N1B4_2539 at 2881.873 kb on + strand, within Pf1N1B4_2539 at 2881.874 kb on - strand, within Pf1N1B4_2539 at 2882.057 kb on - strand at 2882.065 kb on + strand at 2882.065 kb on + strand at 2882.251 kb on + strand at 2882.251 kb on + strand at 2882.252 kb on - strand at 2882.252 kb on - strand at 2882.252 kb on - strand at 2882.252 kb on - strand at 2882.417 kb on - strand, within Pf1N1B4_2540 at 2882.417 kb on - strand, within Pf1N1B4_2540 at 2882.417 kb on - strand, within Pf1N1B4_2540 at 2882.417 kb on - strand, within Pf1N1B4_2540 at 2882.515 kb on + strand, within Pf1N1B4_2540 at 2882.515 kb on + strand, within Pf1N1B4_2540 at 2882.515 kb on + strand, within Pf1N1B4_2540 at 2882.515 kb on + strand, within Pf1N1B4_2540 at 2882.515 kb on + strand, within Pf1N1B4_2540 at 2882.515 kb on + strand, within Pf1N1B4_2540 at 2882.515 kb on + strand, within Pf1N1B4_2540 at 2882.515 kb on + strand, within Pf1N1B4_2540 at 2882.515 kb on + strand, within Pf1N1B4_2540 at 2882.515 kb on + strand, within Pf1N1B4_2540 at 2882.516 kb on - strand, within Pf1N1B4_2540 at 2882.516 kb on - strand, within Pf1N1B4_2540 at 2882.516 kb on - strand, within Pf1N1B4_2540 at 2882.516 kb on - strand, within Pf1N1B4_2540 at 2882.516 kb on - strand, within Pf1N1B4_2540 at 2882.516 kb on - strand, within Pf1N1B4_2540 at 2882.517 kb on + strand, within Pf1N1B4_2540 at 2882.665 kb on + strand, within Pf1N1B4_2540 at 2882.665 kb on + strand, within Pf1N1B4_2540 at 2882.665 kb on + strand, within Pf1N1B4_2540 at 2882.665 kb on + strand, within Pf1N1B4_2540 at 2882.665 kb on + strand, within Pf1N1B4_2540 at 2882.665 kb on + strand, within Pf1N1B4_2540 at 2882.665 kb on + strand, within Pf1N1B4_2540 at 2882.665 kb on + strand, within Pf1N1B4_2540 at 2882.665 kb on + strand, within Pf1N1B4_2540 at 2882.665 kb on + strand, within Pf1N1B4_2540 at 2882.665 kb on + strand, within Pf1N1B4_2540 at 2882.665 kb on + strand, within Pf1N1B4_2540 at 2882.665 kb on + strand, within Pf1N1B4_2540 at 2882.666 kb on - strand, within Pf1N1B4_2540 at 2882.666 kb on - strand, within Pf1N1B4_2540 at 2882.666 kb on - strand, within Pf1N1B4_2540 at 2882.666 kb on - strand at 2882.668 kb on - strand, within Pf1N1B4_2540 at 2882.668 kb on - strand, within Pf1N1B4_2540 at 2882.713 kb on + strand, within Pf1N1B4_2540 at 2882.742 kb on + strand at 2882.742 kb on + strand, within Pf1N1B4_2540 at 2882.755 kb on + strand, within Pf1N1B4_2540 at 2882.755 kb on + strand, within Pf1N1B4_2540 at 2882.755 kb on + strand, within Pf1N1B4_2540 at 2882.755 kb on + strand, within Pf1N1B4_2540 at 2882.755 kb on + strand, within Pf1N1B4_2540 at 2882.756 kb on - strand, within Pf1N1B4_2540 at 2882.758 kb on + strand, within Pf1N1B4_2540 at 2882.759 kb on - strand, within Pf1N1B4_2540 at 2882.833 kb on + strand, within Pf1N1B4_2540 at 2882.833 kb on + strand, within Pf1N1B4_2540 at 2882.833 kb on + strand, within Pf1N1B4_2540 at 2882.834 kb on - strand, within Pf1N1B4_2540 at 2882.915 kb on - strand, within Pf1N1B4_2540
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 2,880,126 + Pf1N1B4_2538 0.25 -1.3 2,880,127 - Pf1N1B4_2538 0.25 -0.2 2,880,127 - Pf1N1B4_2538 0.25 -1.0 2,880,127 - Pf1N1B4_2538 0.25 +0.3 2,880,127 - Pf1N1B4_2538 0.25 +1.8 2,880,127 - Pf1N1B4_2538 0.25 +0.5 2,880,441 + Pf1N1B4_2538 0.53 +1.8 2,880,441 + Pf1N1B4_2538 0.53 +1.2 2,880,441 + Pf1N1B4_2538 0.53 +2.0 2,880,442 - -0.6 2,880,540 + Pf1N1B4_2538 0.62 +1.3 2,880,540 + Pf1N1B4_2538 0.62 -2.0 2,880,541 - Pf1N1B4_2538 0.62 -0.6 2,880,795 + Pf1N1B4_2538 0.85 +0.4 2,880,795 + Pf1N1B4_2538 0.85 +0.0 2,880,795 + Pf1N1B4_2538 0.85 -1.4 2,880,795 + Pf1N1B4_2538 0.85 +0.2 2,880,795 + Pf1N1B4_2538 0.85 -2.6 2,880,795 + Pf1N1B4_2538 0.85 +0.4 2,880,795 + Pf1N1B4_2538 0.85 -0.4 2,880,796 - -1.0 2,880,952 + -1.7 2,881,012 + +0.2 2,881,012 + +0.3 2,881,012 + +0.1 2,881,012 + +0.8 2,881,012 + -0.0 2,881,012 + -0.4 2,881,012 + -0.2 2,881,012 + -0.3 2,881,012 + -0.5 2,881,013 - +1.0 2,881,013 - +0.6 2,881,013 - +2.0 2,881,013 - -0.8 2,881,013 - -0.6 2,881,013 - +0.5 2,881,013 - -2.1 2,881,147 + Pf1N1B4_2539 0.13 -0.5 2,881,148 - Pf1N1B4_2539 0.13 +2.5 2,881,148 - Pf1N1B4_2539 0.13 -1.8 2,881,195 + Pf1N1B4_2539 0.17 +0.3 2,881,195 + Pf1N1B4_2539 0.17 +1.0 2,881,195 + Pf1N1B4_2539 0.17 +0.2 2,881,259 + Pf1N1B4_2539 0.23 -1.0 2,881,260 - +2.4 2,881,348 + Pf1N1B4_2539 0.32 +1.4 2,881,349 - Pf1N1B4_2539 0.32 +1.0 2,881,510 + Pf1N1B4_2539 0.47 +0.8 2,881,510 + Pf1N1B4_2539 0.47 -0.4 2,881,510 + Pf1N1B4_2539 0.47 -1.8 2,881,511 - Pf1N1B4_2539 0.48 +0.2 2,881,561 + Pf1N1B4_2539 0.52 -0.4 2,881,561 + Pf1N1B4_2539 0.52 -0.4 2,881,568 + Pf1N1B4_2539 0.53 -0.6 2,881,568 + Pf1N1B4_2539 0.53 +0.0 2,881,568 + Pf1N1B4_2539 0.53 +0.2 2,881,666 + Pf1N1B4_2539 0.62 -2.1 2,881,702 + Pf1N1B4_2539 0.66 -0.8 2,881,768 + Pf1N1B4_2539 0.72 -1.0 2,881,769 - Pf1N1B4_2539 0.72 +0.4 2,881,873 + Pf1N1B4_2539 0.82 +0.8 2,881,874 - Pf1N1B4_2539 0.82 +0.0 2,882,057 - +1.4 2,882,065 + +0.0 2,882,065 + -2.4 2,882,251 + -0.4 2,882,251 + +0.5 2,882,252 - +0.0 2,882,252 - -1.5 2,882,252 - +0.3 2,882,252 - +0.5 2,882,417 - Pf1N1B4_2540 0.13 -0.5 2,882,417 - Pf1N1B4_2540 0.13 -1.8 2,882,417 - Pf1N1B4_2540 0.13 -0.8 2,882,417 - Pf1N1B4_2540 0.13 -0.1 2,882,515 + Pf1N1B4_2540 0.21 +0.6 2,882,515 + Pf1N1B4_2540 0.21 +0.6 2,882,515 + Pf1N1B4_2540 0.21 -0.5 2,882,515 + Pf1N1B4_2540 0.21 +1.6 2,882,515 + Pf1N1B4_2540 0.21 -0.1 2,882,515 + Pf1N1B4_2540 0.21 -0.1 2,882,515 + Pf1N1B4_2540 0.21 +2.0 2,882,515 + Pf1N1B4_2540 0.21 -0.5 2,882,515 + Pf1N1B4_2540 0.21 -0.1 2,882,515 + Pf1N1B4_2540 0.21 +0.4 2,882,516 - Pf1N1B4_2540 0.21 +0.2 2,882,516 - Pf1N1B4_2540 0.21 +0.6 2,882,516 - Pf1N1B4_2540 0.21 +1.0 2,882,516 - Pf1N1B4_2540 0.21 -1.5 2,882,516 - Pf1N1B4_2540 0.21 +1.0 2,882,516 - Pf1N1B4_2540 0.21 +0.2 2,882,517 + Pf1N1B4_2540 0.21 +0.5 2,882,665 + Pf1N1B4_2540 0.33 +0.2 2,882,665 + Pf1N1B4_2540 0.33 +0.0 2,882,665 + Pf1N1B4_2540 0.33 -1.4 2,882,665 + Pf1N1B4_2540 0.33 -0.3 2,882,665 + Pf1N1B4_2540 0.33 -0.3 2,882,665 + Pf1N1B4_2540 0.33 +0.3 2,882,665 + Pf1N1B4_2540 0.33 +0.4 2,882,665 + Pf1N1B4_2540 0.33 -1.3 2,882,665 + Pf1N1B4_2540 0.33 +1.1 2,882,665 + Pf1N1B4_2540 0.33 +0.8 2,882,665 + Pf1N1B4_2540 0.33 +0.7 2,882,665 + Pf1N1B4_2540 0.33 +0.0 2,882,665 + Pf1N1B4_2540 0.33 +0.7 2,882,666 - Pf1N1B4_2540 0.33 +0.5 2,882,666 - Pf1N1B4_2540 0.33 +2.0 2,882,666 - Pf1N1B4_2540 0.33 +2.2 2,882,666 - -1.3 2,882,668 - Pf1N1B4_2540 0.33 -0.3 2,882,668 - Pf1N1B4_2540 0.33 +1.2 2,882,713 + Pf1N1B4_2540 0.37 +0.7 2,882,742 + +1.0 2,882,742 + Pf1N1B4_2540 0.39 +1.5 2,882,755 + Pf1N1B4_2540 0.40 -0.2 2,882,755 + Pf1N1B4_2540 0.40 +0.1 2,882,755 + Pf1N1B4_2540 0.40 -2.3 2,882,755 + Pf1N1B4_2540 0.40 -1.8 2,882,755 + Pf1N1B4_2540 0.40 -2.1 2,882,756 - Pf1N1B4_2540 0.40 -0.3 2,882,758 + Pf1N1B4_2540 0.40 -1.5 2,882,759 - Pf1N1B4_2540 0.40 -0.1 2,882,833 + Pf1N1B4_2540 0.46 -0.1 2,882,833 + Pf1N1B4_2540 0.46 -0.8 2,882,833 + Pf1N1B4_2540 0.46 -1.8 2,882,834 - Pf1N1B4_2540 0.46 +0.0 2,882,915 - Pf1N1B4_2540 0.53 -1.0
Or see this region's nucleotide sequence