Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2537 and Pf1N1B4_2538 are separated by 327 nucleotides Pf1N1B4_2538 and Pf1N1B4_2539 are separated by 49 nucleotides
Pf1N1B4_2537 - DNA-binding response regulator, LuxR family, near polyamine transporter, at 2,878,872 to 2,879,513
_2537
Pf1N1B4_2538 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 2,879,841 to 2,880,965
_2538
Pf1N1B4_2539 - ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1), at 2,881,015 to 2,882,058
_2539
Position (kb)
2879
2880
2881 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2878.847 kb on - strand at 2878.847 kb on - strand at 2878.864 kb on + strand at 2878.864 kb on + strand at 2878.864 kb on + strand at 2878.864 kb on + strand at 2878.864 kb on + strand at 2878.864 kb on + strand at 2878.864 kb on + strand at 2878.865 kb on - strand at 2878.865 kb on - strand at 2878.865 kb on - strand at 2878.865 kb on - strand at 2878.865 kb on - strand at 2878.865 kb on - strand at 2878.894 kb on + strand at 2878.894 kb on + strand at 2878.894 kb on + strand at 2878.895 kb on - strand at 2878.895 kb on - strand at 2879.046 kb on - strand, within Pf1N1B4_2537 at 2879.046 kb on - strand, within Pf1N1B4_2537 at 2879.185 kb on - strand, within Pf1N1B4_2537 at 2879.253 kb on + strand, within Pf1N1B4_2537 at 2879.254 kb on - strand, within Pf1N1B4_2537 at 2879.254 kb on - strand, within Pf1N1B4_2537 at 2879.487 kb on + strand at 2879.487 kb on + strand at 2879.488 kb on - strand at 2879.488 kb on - strand at 2879.488 kb on - strand at 2879.653 kb on - strand at 2879.717 kb on - strand at 2879.717 kb on - strand at 2879.799 kb on + strand at 2879.800 kb on - strand at 2879.805 kb on + strand at 2879.806 kb on - strand at 2879.911 kb on - strand at 2879.911 kb on - strand at 2880.126 kb on + strand, within Pf1N1B4_2538 at 2880.127 kb on - strand, within Pf1N1B4_2538 at 2880.127 kb on - strand, within Pf1N1B4_2538 at 2880.127 kb on - strand, within Pf1N1B4_2538 at 2880.127 kb on - strand, within Pf1N1B4_2538 at 2880.127 kb on - strand, within Pf1N1B4_2538 at 2880.441 kb on + strand, within Pf1N1B4_2538 at 2880.441 kb on + strand, within Pf1N1B4_2538 at 2880.441 kb on + strand, within Pf1N1B4_2538 at 2880.442 kb on - strand at 2880.540 kb on + strand, within Pf1N1B4_2538 at 2880.540 kb on + strand, within Pf1N1B4_2538 at 2880.541 kb on - strand, within Pf1N1B4_2538 at 2880.795 kb on + strand, within Pf1N1B4_2538 at 2880.795 kb on + strand, within Pf1N1B4_2538 at 2880.795 kb on + strand, within Pf1N1B4_2538 at 2880.795 kb on + strand, within Pf1N1B4_2538 at 2880.795 kb on + strand, within Pf1N1B4_2538 at 2880.795 kb on + strand, within Pf1N1B4_2538 at 2880.795 kb on + strand, within Pf1N1B4_2538 at 2880.796 kb on - strand at 2880.952 kb on + strand at 2881.012 kb on + strand at 2881.012 kb on + strand at 2881.012 kb on + strand at 2881.012 kb on + strand at 2881.012 kb on + strand at 2881.012 kb on + strand at 2881.012 kb on + strand at 2881.012 kb on + strand at 2881.012 kb on + strand at 2881.013 kb on - strand at 2881.013 kb on - strand at 2881.013 kb on - strand at 2881.013 kb on - strand at 2881.013 kb on - strand at 2881.013 kb on - strand at 2881.013 kb on - strand at 2881.147 kb on + strand, within Pf1N1B4_2539 at 2881.148 kb on - strand, within Pf1N1B4_2539 at 2881.148 kb on - strand, within Pf1N1B4_2539 at 2881.195 kb on + strand, within Pf1N1B4_2539 at 2881.195 kb on + strand, within Pf1N1B4_2539 at 2881.195 kb on + strand, within Pf1N1B4_2539 at 2881.259 kb on + strand, within Pf1N1B4_2539 at 2881.260 kb on - strand at 2881.348 kb on + strand, within Pf1N1B4_2539 at 2881.349 kb on - strand, within Pf1N1B4_2539 at 2881.510 kb on + strand, within Pf1N1B4_2539 at 2881.510 kb on + strand, within Pf1N1B4_2539 at 2881.510 kb on + strand, within Pf1N1B4_2539 at 2881.511 kb on - strand, within Pf1N1B4_2539 at 2881.561 kb on + strand, within Pf1N1B4_2539 at 2881.561 kb on + strand, within Pf1N1B4_2539 at 2881.568 kb on + strand, within Pf1N1B4_2539 at 2881.568 kb on + strand, within Pf1N1B4_2539 at 2881.568 kb on + strand, within Pf1N1B4_2539 at 2881.666 kb on + strand, within Pf1N1B4_2539 at 2881.702 kb on + strand, within Pf1N1B4_2539 at 2881.768 kb on + strand, within Pf1N1B4_2539 at 2881.769 kb on - strand, within Pf1N1B4_2539 at 2881.873 kb on + strand, within Pf1N1B4_2539 at 2881.874 kb on - strand, within Pf1N1B4_2539
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 2,878,847 - +2.0 2,878,847 - +0.0 2,878,864 + +1.0 2,878,864 + +0.2 2,878,864 + -1.3 2,878,864 + -0.3 2,878,864 + +0.1 2,878,864 + -0.6 2,878,864 + +1.0 2,878,865 - +0.7 2,878,865 - +0.4 2,878,865 - +0.9 2,878,865 - -0.8 2,878,865 - -0.3 2,878,865 - +0.3 2,878,894 + +0.6 2,878,894 + -1.7 2,878,894 + -0.3 2,878,895 - -0.3 2,878,895 - -1.0 2,879,046 - Pf1N1B4_2537 0.27 +1.5 2,879,046 - Pf1N1B4_2537 0.27 +1.3 2,879,185 - Pf1N1B4_2537 0.49 -0.9 2,879,253 + Pf1N1B4_2537 0.59 +0.2 2,879,254 - Pf1N1B4_2537 0.60 -0.8 2,879,254 - Pf1N1B4_2537 0.60 +0.6 2,879,487 + -0.6 2,879,487 + -0.7 2,879,488 - +0.2 2,879,488 - -2.3 2,879,488 - +1.0 2,879,653 - -1.1 2,879,717 - -0.3 2,879,717 - -0.8 2,879,799 + +0.4 2,879,800 - -0.8 2,879,805 + -1.3 2,879,806 - +3.0 2,879,911 - +1.0 2,879,911 - -1.0 2,880,126 + Pf1N1B4_2538 0.25 -1.3 2,880,127 - Pf1N1B4_2538 0.25 -0.2 2,880,127 - Pf1N1B4_2538 0.25 -1.0 2,880,127 - Pf1N1B4_2538 0.25 +0.3 2,880,127 - Pf1N1B4_2538 0.25 +1.8 2,880,127 - Pf1N1B4_2538 0.25 +0.5 2,880,441 + Pf1N1B4_2538 0.53 +1.8 2,880,441 + Pf1N1B4_2538 0.53 +1.2 2,880,441 + Pf1N1B4_2538 0.53 +2.0 2,880,442 - -0.6 2,880,540 + Pf1N1B4_2538 0.62 +1.3 2,880,540 + Pf1N1B4_2538 0.62 -2.0 2,880,541 - Pf1N1B4_2538 0.62 -0.6 2,880,795 + Pf1N1B4_2538 0.85 +0.4 2,880,795 + Pf1N1B4_2538 0.85 +0.0 2,880,795 + Pf1N1B4_2538 0.85 -1.4 2,880,795 + Pf1N1B4_2538 0.85 +0.2 2,880,795 + Pf1N1B4_2538 0.85 -2.6 2,880,795 + Pf1N1B4_2538 0.85 +0.4 2,880,795 + Pf1N1B4_2538 0.85 -0.4 2,880,796 - -1.0 2,880,952 + -1.7 2,881,012 + +0.2 2,881,012 + +0.3 2,881,012 + +0.1 2,881,012 + +0.8 2,881,012 + -0.0 2,881,012 + -0.4 2,881,012 + -0.2 2,881,012 + -0.3 2,881,012 + -0.5 2,881,013 - +1.0 2,881,013 - +0.6 2,881,013 - +2.0 2,881,013 - -0.8 2,881,013 - -0.6 2,881,013 - +0.5 2,881,013 - -2.1 2,881,147 + Pf1N1B4_2539 0.13 -0.5 2,881,148 - Pf1N1B4_2539 0.13 +2.5 2,881,148 - Pf1N1B4_2539 0.13 -1.8 2,881,195 + Pf1N1B4_2539 0.17 +0.3 2,881,195 + Pf1N1B4_2539 0.17 +1.0 2,881,195 + Pf1N1B4_2539 0.17 +0.2 2,881,259 + Pf1N1B4_2539 0.23 -1.0 2,881,260 - +2.4 2,881,348 + Pf1N1B4_2539 0.32 +1.4 2,881,349 - Pf1N1B4_2539 0.32 +1.0 2,881,510 + Pf1N1B4_2539 0.47 +0.8 2,881,510 + Pf1N1B4_2539 0.47 -0.4 2,881,510 + Pf1N1B4_2539 0.47 -1.8 2,881,511 - Pf1N1B4_2539 0.48 +0.2 2,881,561 + Pf1N1B4_2539 0.52 -0.4 2,881,561 + Pf1N1B4_2539 0.52 -0.4 2,881,568 + Pf1N1B4_2539 0.53 -0.6 2,881,568 + Pf1N1B4_2539 0.53 +0.0 2,881,568 + Pf1N1B4_2539 0.53 +0.2 2,881,666 + Pf1N1B4_2539 0.62 -2.1 2,881,702 + Pf1N1B4_2539 0.66 -0.8 2,881,768 + Pf1N1B4_2539 0.72 -1.0 2,881,769 - Pf1N1B4_2539 0.72 +0.4 2,881,873 + Pf1N1B4_2539 0.82 +0.8 2,881,874 - Pf1N1B4_2539 0.82 +0.0
Or see this region's nucleotide sequence