Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2239 and Pf1N1B4_2240 are separated by 126 nucleotides Pf1N1B4_2240 and Pf1N1B4_2241 are separated by 124 nucleotides Pf1N1B4_2241 and Pf1N1B4_2242 are separated by 316 nucleotides
Pf1N1B4_2239 - FIG00953395: hypothetical protein, at 2,554,248 to 2,554,586
_2239
Pf1N1B4_2240 - Transcriptional regulator, AsnC family, at 2,554,713 to 2,555,177
_2240
Pf1N1B4_2241 - Adenylate cyclase (EC 4.6.1.1), at 2,555,302 to 2,556,669
_2241
Pf1N1B4_2242 - Acetylornithine deacetylase (EC 3.5.1.16), at 2,556,986 to 2,558,155
_2242
Position (kb)
2555
2556
2557 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2554.326 kb on - strand, within Pf1N1B4_2239 at 2554.326 kb on - strand, within Pf1N1B4_2239 at 2554.329 kb on + strand, within Pf1N1B4_2239 at 2554.330 kb on - strand, within Pf1N1B4_2239 at 2554.330 kb on - strand, within Pf1N1B4_2239 at 2554.497 kb on + strand, within Pf1N1B4_2239 at 2554.497 kb on + strand, within Pf1N1B4_2239 at 2554.498 kb on - strand, within Pf1N1B4_2239 at 2554.498 kb on - strand, within Pf1N1B4_2239 at 2554.498 kb on - strand, within Pf1N1B4_2239 at 2554.498 kb on - strand, within Pf1N1B4_2239 at 2554.498 kb on - strand, within Pf1N1B4_2239 at 2554.498 kb on - strand, within Pf1N1B4_2239 at 2554.504 kb on + strand, within Pf1N1B4_2239 at 2554.504 kb on + strand, within Pf1N1B4_2239 at 2554.575 kb on + strand at 2554.575 kb on + strand at 2554.575 kb on + strand at 2554.575 kb on + strand at 2554.576 kb on - strand at 2554.641 kb on + strand at 2554.645 kb on + strand at 2554.645 kb on + strand at 2554.645 kb on + strand at 2554.646 kb on - strand at 2554.646 kb on - strand at 2554.691 kb on - strand at 2554.691 kb on - strand at 2554.702 kb on + strand at 2554.702 kb on + strand at 2554.712 kb on + strand at 2555.010 kb on + strand, within Pf1N1B4_2240 at 2555.011 kb on - strand, within Pf1N1B4_2240 at 2555.011 kb on - strand, within Pf1N1B4_2240 at 2555.011 kb on - strand, within Pf1N1B4_2240 at 2555.070 kb on + strand, within Pf1N1B4_2240 at 2555.070 kb on + strand, within Pf1N1B4_2240 at 2555.070 kb on + strand, within Pf1N1B4_2240 at 2555.070 kb on + strand, within Pf1N1B4_2240 at 2555.071 kb on - strand, within Pf1N1B4_2240 at 2555.071 kb on - strand, within Pf1N1B4_2240 at 2555.250 kb on + strand at 2555.250 kb on + strand at 2555.250 kb on + strand at 2555.303 kb on + strand at 2555.303 kb on + strand at 2555.304 kb on - strand at 2555.339 kb on + strand at 2555.339 kb on + strand at 2555.340 kb on - strand at 2555.429 kb on + strand at 2555.430 kb on - strand at 2555.430 kb on - strand at 2555.430 kb on - strand at 2555.430 kb on - strand at 2555.742 kb on + strand, within Pf1N1B4_2241 at 2555.742 kb on + strand, within Pf1N1B4_2241 at 2555.742 kb on + strand, within Pf1N1B4_2241 at 2555.743 kb on - strand, within Pf1N1B4_2241 at 2555.743 kb on - strand at 2555.746 kb on + strand, within Pf1N1B4_2241 at 2555.746 kb on + strand at 2555.746 kb on + strand, within Pf1N1B4_2241 at 2555.746 kb on + strand, within Pf1N1B4_2241 at 2555.746 kb on + strand, within Pf1N1B4_2241 at 2555.746 kb on + strand, within Pf1N1B4_2241 at 2555.747 kb on - strand, within Pf1N1B4_2241 at 2555.747 kb on - strand, within Pf1N1B4_2241 at 2555.747 kb on - strand, within Pf1N1B4_2241 at 2555.747 kb on - strand, within Pf1N1B4_2241 at 2555.747 kb on - strand, within Pf1N1B4_2241 at 2555.747 kb on - strand, within Pf1N1B4_2241 at 2555.773 kb on + strand, within Pf1N1B4_2241 at 2555.774 kb on - strand, within Pf1N1B4_2241 at 2555.774 kb on - strand, within Pf1N1B4_2241 at 2555.933 kb on + strand, within Pf1N1B4_2241 at 2556.044 kb on + strand, within Pf1N1B4_2241 at 2556.045 kb on - strand, within Pf1N1B4_2241 at 2556.045 kb on - strand, within Pf1N1B4_2241 at 2556.139 kb on + strand, within Pf1N1B4_2241 at 2556.140 kb on - strand, within Pf1N1B4_2241 at 2556.140 kb on - strand, within Pf1N1B4_2241 at 2556.414 kb on - strand, within Pf1N1B4_2241 at 2556.414 kb on - strand, within Pf1N1B4_2241 at 2556.545 kb on + strand at 2556.545 kb on + strand at 2556.545 kb on + strand at 2556.545 kb on + strand at 2556.734 kb on + strand at 2556.734 kb on + strand at 2556.734 kb on + strand at 2556.734 kb on + strand at 2556.735 kb on - strand at 2556.735 kb on - strand at 2556.735 kb on - strand at 2556.735 kb on - strand at 2556.735 kb on - strand at 2556.735 kb on - strand at 2556.735 kb on - strand at 2556.753 kb on - strand at 2556.822 kb on + strand at 2556.822 kb on + strand at 2556.822 kb on + strand at 2556.822 kb on + strand at 2556.822 kb on + strand at 2556.823 kb on - strand at 2556.823 kb on - strand at 2556.928 kb on + strand at 2556.928 kb on + strand at 2556.929 kb on - strand at 2556.929 kb on - strand at 2556.929 kb on - strand at 2556.950 kb on + strand at 2556.950 kb on + strand at 2556.950 kb on + strand at 2556.950 kb on + strand at 2556.950 kb on + strand at 2556.950 kb on + strand at 2556.950 kb on + strand at 2556.950 kb on + strand at 2556.950 kb on + strand at 2556.950 kb on + strand at 2556.950 kb on + strand at 2556.950 kb on + strand at 2556.950 kb on + strand at 2556.950 kb on + strand at 2556.951 kb on - strand at 2556.951 kb on - strand at 2556.951 kb on - strand at 2556.951 kb on - strand at 2556.951 kb on - strand at 2556.951 kb on - strand at 2556.951 kb on - strand at 2556.951 kb on - strand at 2556.951 kb on - strand at 2556.951 kb on - strand at 2556.951 kb on - strand at 2556.951 kb on - strand at 2556.951 kb on - strand at 2557.219 kb on + strand, within Pf1N1B4_2242 at 2557.219 kb on + strand, within Pf1N1B4_2242 at 2557.220 kb on - strand, within Pf1N1B4_2242 at 2557.220 kb on - strand, within Pf1N1B4_2242 at 2557.455 kb on + strand, within Pf1N1B4_2242 at 2557.455 kb on + strand, within Pf1N1B4_2242 at 2557.456 kb on - strand, within Pf1N1B4_2242 at 2557.456 kb on - strand, within Pf1N1B4_2242 at 2557.456 kb on - strand, within Pf1N1B4_2242
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 2,554,326 - Pf1N1B4_2239 0.23 -0.2 2,554,326 - Pf1N1B4_2239 0.23 -0.2 2,554,329 + Pf1N1B4_2239 0.24 +0.5 2,554,330 - Pf1N1B4_2239 0.24 -0.0 2,554,330 - Pf1N1B4_2239 0.24 +0.2 2,554,497 + Pf1N1B4_2239 0.73 -1.6 2,554,497 + Pf1N1B4_2239 0.73 -1.6 2,554,498 - Pf1N1B4_2239 0.74 -1.0 2,554,498 - Pf1N1B4_2239 0.74 -1.3 2,554,498 - Pf1N1B4_2239 0.74 -0.2 2,554,498 - Pf1N1B4_2239 0.74 +0.3 2,554,498 - Pf1N1B4_2239 0.74 -0.4 2,554,498 - Pf1N1B4_2239 0.74 +2.0 2,554,504 + Pf1N1B4_2239 0.76 -0.3 2,554,504 + Pf1N1B4_2239 0.76 +0.1 2,554,575 + +1.7 2,554,575 + +2.2 2,554,575 + -0.3 2,554,575 + +3.2 2,554,576 - +1.5 2,554,641 + -0.1 2,554,645 + +0.5 2,554,645 + -1.3 2,554,645 + +0.7 2,554,646 - -0.2 2,554,646 - +0.3 2,554,691 - -1.3 2,554,691 - -0.4 2,554,702 + -1.2 2,554,702 + -1.8 2,554,712 + -1.6 2,555,010 + Pf1N1B4_2240 0.64 -0.0 2,555,011 - Pf1N1B4_2240 0.64 +0.5 2,555,011 - Pf1N1B4_2240 0.64 +0.2 2,555,011 - Pf1N1B4_2240 0.64 -1.5 2,555,070 + Pf1N1B4_2240 0.77 +1.6 2,555,070 + Pf1N1B4_2240 0.77 +1.2 2,555,070 + Pf1N1B4_2240 0.77 +0.2 2,555,070 + Pf1N1B4_2240 0.77 -1.4 2,555,071 - Pf1N1B4_2240 0.77 +1.4 2,555,071 - Pf1N1B4_2240 0.77 +1.3 2,555,250 + +0.2 2,555,250 + +1.2 2,555,250 + -1.1 2,555,303 + -0.8 2,555,303 + +1.2 2,555,304 - -0.3 2,555,339 + -1.2 2,555,339 + +1.9 2,555,340 - +0.7 2,555,429 + -0.2 2,555,430 - -0.4 2,555,430 - -2.0 2,555,430 - +0.2 2,555,430 - -0.1 2,555,742 + Pf1N1B4_2241 0.32 -2.0 2,555,742 + Pf1N1B4_2241 0.32 -2.2 2,555,742 + Pf1N1B4_2241 0.32 -1.2 2,555,743 - Pf1N1B4_2241 0.32 +1.0 2,555,743 - -0.4 2,555,746 + Pf1N1B4_2241 0.32 -0.2 2,555,746 + -0.4 2,555,746 + Pf1N1B4_2241 0.32 -1.4 2,555,746 + Pf1N1B4_2241 0.32 +0.8 2,555,746 + Pf1N1B4_2241 0.32 -1.4 2,555,746 + Pf1N1B4_2241 0.32 -2.0 2,555,747 - Pf1N1B4_2241 0.33 -0.8 2,555,747 - Pf1N1B4_2241 0.33 -1.0 2,555,747 - Pf1N1B4_2241 0.33 -1.8 2,555,747 - Pf1N1B4_2241 0.33 -0.3 2,555,747 - Pf1N1B4_2241 0.33 -1.6 2,555,747 - Pf1N1B4_2241 0.33 -0.3 2,555,773 + Pf1N1B4_2241 0.34 +1.2 2,555,774 - Pf1N1B4_2241 0.35 +0.1 2,555,774 - Pf1N1B4_2241 0.35 +0.4 2,555,933 + Pf1N1B4_2241 0.46 -1.4 2,556,044 + Pf1N1B4_2241 0.54 -1.2 2,556,045 - Pf1N1B4_2241 0.54 -0.2 2,556,045 - Pf1N1B4_2241 0.54 -0.3 2,556,139 + Pf1N1B4_2241 0.61 -1.4 2,556,140 - Pf1N1B4_2241 0.61 -1.2 2,556,140 - Pf1N1B4_2241 0.61 -0.8 2,556,414 - Pf1N1B4_2241 0.81 +1.2 2,556,414 - Pf1N1B4_2241 0.81 -0.2 2,556,545 + -0.2 2,556,545 + +1.2 2,556,545 + +1.2 2,556,545 + +1.2 2,556,734 + -1.0 2,556,734 + -0.4 2,556,734 + +1.0 2,556,734 + -1.3 2,556,735 - +0.2 2,556,735 - -1.2 2,556,735 - +0.6 2,556,735 - -0.0 2,556,735 - +0.4 2,556,735 - -0.4 2,556,735 - -1.4 2,556,753 - +2.6 2,556,822 + +2.4 2,556,822 + -1.1 2,556,822 + -1.8 2,556,822 + -0.4 2,556,822 + -0.4 2,556,823 - +0.4 2,556,823 - +0.8 2,556,928 + +0.4 2,556,928 + -1.9 2,556,929 - +0.0 2,556,929 - +0.8 2,556,929 - -0.4 2,556,950 + -0.4 2,556,950 + -0.2 2,556,950 + +0.6 2,556,950 + +0.2 2,556,950 + +1.9 2,556,950 + -1.3 2,556,950 + +0.4 2,556,950 + +0.8 2,556,950 + +1.4 2,556,950 + -0.5 2,556,950 + +0.2 2,556,950 + +1.5 2,556,950 + +0.2 2,556,950 + -0.4 2,556,951 - -1.6 2,556,951 - +0.2 2,556,951 - +1.2 2,556,951 - -1.2 2,556,951 - -1.0 2,556,951 - +3.0 2,556,951 - +0.4 2,556,951 - -0.8 2,556,951 - -1.0 2,556,951 - -1.1 2,556,951 - +0.1 2,556,951 - -0.4 2,556,951 - +0.1 2,557,219 + Pf1N1B4_2242 0.20 -1.2 2,557,219 + Pf1N1B4_2242 0.20 +1.9 2,557,220 - Pf1N1B4_2242 0.20 +0.6 2,557,220 - Pf1N1B4_2242 0.20 +0.4 2,557,455 + Pf1N1B4_2242 0.40 +0.2 2,557,455 + Pf1N1B4_2242 0.40 -1.2 2,557,456 - Pf1N1B4_2242 0.40 +0.6 2,557,456 - Pf1N1B4_2242 0.40 -0.1 2,557,456 - Pf1N1B4_2242 0.40 -0.0
Or see this region's nucleotide sequence