Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2058 and Pf1N1B4_2059 overlap by 4 nucleotides Pf1N1B4_2059 and Pf1N1B4_2060 overlap by 7 nucleotides Pf1N1B4_2060 and Pf1N1B4_2061 are separated by 577 nucleotides
Pf1N1B4_2058 - Protein ImpG/VasA, at 2,350,276 to 2,352,135
_2058
Pf1N1B4_2059 - Uncharacterized protein ImpF, at 2,352,132 to 2,352,644
_2059
Pf1N1B4_2060 - hypothetical protein, at 2,352,638 to 2,353,063
_2060
Pf1N1B4_2061 - hypothetical protein, at 2,353,641 to 2,354,078
_2061
Position (kb)
2352
2353
2354 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2351.694 kb on - strand, within Pf1N1B4_2058 at 2351.694 kb on - strand, within Pf1N1B4_2058 at 2351.888 kb on + strand, within Pf1N1B4_2058 at 2351.889 kb on - strand, within Pf1N1B4_2058 at 2351.956 kb on + strand at 2351.957 kb on - strand at 2352.002 kb on + strand at 2352.002 kb on + strand at 2352.110 kb on + strand at 2352.110 kb on + strand at 2352.110 kb on + strand at 2352.110 kb on + strand at 2352.110 kb on + strand at 2352.111 kb on - strand at 2352.111 kb on - strand at 2352.111 kb on - strand at 2352.111 kb on - strand at 2352.111 kb on - strand at 2352.111 kb on - strand at 2352.646 kb on + strand at 2352.646 kb on + strand at 2352.647 kb on - strand at 2352.647 kb on - strand at 2352.660 kb on + strand at 2352.660 kb on + strand at 2352.660 kb on + strand at 2352.660 kb on + strand at 2352.661 kb on - strand at 2352.661 kb on - strand at 2352.661 kb on - strand at 2352.661 kb on - strand at 2352.661 kb on - strand at 2352.661 kb on - strand at 2352.661 kb on - strand at 2352.661 kb on - strand at 2352.703 kb on + strand, within Pf1N1B4_2060 at 2352.743 kb on - strand, within Pf1N1B4_2060 at 2352.854 kb on - strand, within Pf1N1B4_2060 at 2353.210 kb on + strand at 2353.211 kb on - strand at 2353.218 kb on + strand at 2353.320 kb on + strand at 2353.396 kb on + strand at 2353.430 kb on + strand at 2353.431 kb on - strand at 2353.431 kb on - strand at 2353.507 kb on + strand at 2353.508 kb on - strand at 2353.512 kb on + strand at 2353.512 kb on + strand at 2353.512 kb on + strand at 2353.512 kb on + strand at 2353.513 kb on - strand at 2353.513 kb on - strand at 2353.640 kb on - strand at 2353.719 kb on + strand, within Pf1N1B4_2061 at 2353.719 kb on + strand, within Pf1N1B4_2061 at 2353.770 kb on - strand, within Pf1N1B4_2061 at 2353.770 kb on - strand, within Pf1N1B4_2061 at 2353.933 kb on + strand, within Pf1N1B4_2061 at 2353.933 kb on + strand, within Pf1N1B4_2061 at 2353.934 kb on - strand, within Pf1N1B4_2061 at 2353.934 kb on - strand, within Pf1N1B4_2061 at 2353.985 kb on + strand, within Pf1N1B4_2061 at 2353.986 kb on - strand, within Pf1N1B4_2061 at 2354.057 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 2,351,694 - Pf1N1B4_2058 0.76 -2.7 2,351,694 - Pf1N1B4_2058 0.76 -0.7 2,351,888 + Pf1N1B4_2058 0.87 +0.4 2,351,889 - Pf1N1B4_2058 0.87 +1.3 2,351,956 + +1.3 2,351,957 - -0.3 2,352,002 + -0.6 2,352,002 + +0.6 2,352,110 + -0.2 2,352,110 + -1.0 2,352,110 + +0.7 2,352,110 + +1.0 2,352,110 + -1.0 2,352,111 - -0.7 2,352,111 - -0.7 2,352,111 - -0.0 2,352,111 - -0.3 2,352,111 - -0.7 2,352,111 - -0.4 2,352,646 + +1.3 2,352,646 + -0.3 2,352,647 - +1.9 2,352,647 - +0.9 2,352,660 + -1.0 2,352,660 + +0.1 2,352,660 + +1.3 2,352,660 + +0.7 2,352,661 - +0.5 2,352,661 - -1.9 2,352,661 - +3.6 2,352,661 - -0.4 2,352,661 - +1.3 2,352,661 - +0.2 2,352,661 - +0.3 2,352,661 - -1.0 2,352,703 + Pf1N1B4_2060 0.15 -0.5 2,352,743 - Pf1N1B4_2060 0.25 -2.0 2,352,854 - Pf1N1B4_2060 0.51 -1.5 2,353,210 + -0.7 2,353,211 - +0.5 2,353,218 + -0.3 2,353,320 + +1.9 2,353,396 + -0.7 2,353,430 + -1.0 2,353,431 - +1.0 2,353,431 - -0.3 2,353,507 + -0.7 2,353,508 - +0.6 2,353,512 + -0.2 2,353,512 + -2.9 2,353,512 + -0.4 2,353,512 + -0.9 2,353,513 - -0.8 2,353,513 - -1.0 2,353,640 - +1.8 2,353,719 + Pf1N1B4_2061 0.18 -1.0 2,353,719 + Pf1N1B4_2061 0.18 -0.4 2,353,770 - Pf1N1B4_2061 0.29 -0.0 2,353,770 - Pf1N1B4_2061 0.29 +0.5 2,353,933 + Pf1N1B4_2061 0.67 +0.7 2,353,933 + Pf1N1B4_2061 0.67 +1.3 2,353,934 - Pf1N1B4_2061 0.67 +0.1 2,353,934 - Pf1N1B4_2061 0.67 +2.6 2,353,985 + Pf1N1B4_2061 0.79 +0.3 2,353,986 - Pf1N1B4_2061 0.79 -1.6 2,354,057 + +0.4
Or see this region's nucleotide sequence