Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2012 and Pf1N1B4_2013 are separated by 47 nucleotides Pf1N1B4_2013 and Pf1N1B4_2014 are separated by 111 nucleotides Pf1N1B4_2014 and Pf1N1B4_2015 are separated by 99 nucleotides Pf1N1B4_2015 and Pf1N1B4_2016 are separated by 17 nucleotides
Pf1N1B4_2012 - Amino acid ABC transporter, permease protein, at 2,298,879 to 2,299,661
_2012
Pf1N1B4_2013 - Lysine-arginine-ornithine-binding periplasmic protein precursor (TC 3.A.1.3.1), at 2,299,709 to 2,300,530
_2013
Pf1N1B4_2014 - Homocysteine S-methyltransferase (EC 2.1.1.10), at 2,300,642 to 2,301,541
_2014
Pf1N1B4_2015 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 2,301,641 to 2,302,309
_2015
Pf1N1B4_2016 - N-acetylglutamate synthase (EC 2.3.1.1), at 2,302,327 to 2,302,773
_2016
Position (kb)
2300
2301
2302 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2299.851 kb on + strand at 2299.989 kb on + strand, within Pf1N1B4_2013 at 2299.989 kb on + strand, within Pf1N1B4_2013 at 2300.070 kb on + strand, within Pf1N1B4_2013 at 2300.079 kb on + strand, within Pf1N1B4_2013 at 2300.079 kb on + strand, within Pf1N1B4_2013 at 2300.079 kb on + strand, within Pf1N1B4_2013 at 2300.079 kb on + strand, within Pf1N1B4_2013 at 2300.079 kb on + strand, within Pf1N1B4_2013 at 2300.080 kb on - strand, within Pf1N1B4_2013 at 2300.080 kb on - strand, within Pf1N1B4_2013 at 2300.137 kb on + strand, within Pf1N1B4_2013 at 2300.137 kb on + strand, within Pf1N1B4_2013 at 2300.137 kb on + strand, within Pf1N1B4_2013 at 2300.137 kb on + strand, within Pf1N1B4_2013 at 2300.137 kb on + strand at 2300.137 kb on + strand, within Pf1N1B4_2013 at 2300.138 kb on - strand, within Pf1N1B4_2013 at 2300.138 kb on - strand, within Pf1N1B4_2013 at 2300.138 kb on - strand, within Pf1N1B4_2013 at 2300.138 kb on - strand, within Pf1N1B4_2013 at 2300.181 kb on + strand, within Pf1N1B4_2013 at 2300.181 kb on + strand, within Pf1N1B4_2013 at 2300.181 kb on + strand, within Pf1N1B4_2013 at 2300.181 kb on + strand, within Pf1N1B4_2013 at 2300.182 kb on - strand, within Pf1N1B4_2013 at 2300.182 kb on - strand, within Pf1N1B4_2013 at 2300.184 kb on + strand, within Pf1N1B4_2013 at 2300.184 kb on + strand, within Pf1N1B4_2013 at 2300.184 kb on + strand, within Pf1N1B4_2013 at 2300.184 kb on + strand, within Pf1N1B4_2013 at 2300.185 kb on - strand, within Pf1N1B4_2013 at 2300.185 kb on - strand, within Pf1N1B4_2013 at 2300.351 kb on + strand, within Pf1N1B4_2013 at 2300.404 kb on - strand, within Pf1N1B4_2013 at 2300.580 kb on - strand at 2300.587 kb on + strand at 2300.587 kb on + strand at 2300.587 kb on + strand at 2300.588 kb on - strand at 2300.589 kb on + strand at 2300.590 kb on - strand at 2300.590 kb on - strand at 2300.783 kb on + strand, within Pf1N1B4_2014 at 2300.783 kb on + strand, within Pf1N1B4_2014 at 2300.783 kb on + strand, within Pf1N1B4_2014 at 2300.784 kb on - strand, within Pf1N1B4_2014 at 2300.784 kb on - strand, within Pf1N1B4_2014 at 2300.784 kb on - strand, within Pf1N1B4_2014 at 2300.785 kb on + strand, within Pf1N1B4_2014 at 2300.785 kb on + strand, within Pf1N1B4_2014 at 2300.786 kb on - strand, within Pf1N1B4_2014 at 2300.822 kb on + strand, within Pf1N1B4_2014 at 2300.843 kb on - strand, within Pf1N1B4_2014 at 2300.843 kb on - strand, within Pf1N1B4_2014 at 2301.434 kb on + strand, within Pf1N1B4_2014 at 2301.511 kb on + strand at 2301.511 kb on + strand at 2301.511 kb on + strand at 2301.512 kb on - strand at 2301.512 kb on - strand at 2301.512 kb on - strand at 2301.579 kb on + strand at 2301.579 kb on + strand at 2301.579 kb on + strand at 2301.579 kb on + strand at 2301.579 kb on + strand at 2301.580 kb on - strand at 2301.601 kb on + strand at 2301.601 kb on + strand at 2301.602 kb on - strand at 2301.602 kb on - strand at 2301.602 kb on - strand at 2301.632 kb on - strand at 2301.632 kb on - strand at 2301.840 kb on + strand, within Pf1N1B4_2015 at 2301.840 kb on + strand, within Pf1N1B4_2015 at 2301.841 kb on - strand, within Pf1N1B4_2015 at 2301.841 kb on - strand, within Pf1N1B4_2015 at 2301.921 kb on + strand, within Pf1N1B4_2015 at 2302.071 kb on + strand, within Pf1N1B4_2015 at 2302.071 kb on + strand, within Pf1N1B4_2015 at 2302.072 kb on - strand, within Pf1N1B4_2015 at 2302.072 kb on - strand, within Pf1N1B4_2015 at 2302.149 kb on + strand, within Pf1N1B4_2015 at 2302.149 kb on + strand, within Pf1N1B4_2015 at 2302.150 kb on - strand, within Pf1N1B4_2015 at 2302.328 kb on + strand at 2302.329 kb on - strand at 2302.329 kb on - strand at 2302.329 kb on - strand at 2302.329 kb on - strand at 2302.329 kb on - strand at 2302.329 kb on - strand at 2302.329 kb on - strand at 2302.329 kb on - strand at 2302.330 kb on + strand at 2302.330 kb on + strand at 2302.330 kb on + strand at 2302.330 kb on + strand at 2302.331 kb on - strand at 2302.331 kb on - strand at 2302.331 kb on - strand at 2302.331 kb on - strand at 2302.331 kb on - strand at 2302.331 kb on - strand at 2302.331 kb on - strand at 2302.331 kb on - strand at 2302.331 kb on - strand at 2302.379 kb on + strand, within Pf1N1B4_2016 at 2302.380 kb on - strand, within Pf1N1B4_2016 at 2302.380 kb on - strand, within Pf1N1B4_2016 at 2302.380 kb on - strand, within Pf1N1B4_2016 at 2302.458 kb on - strand, within Pf1N1B4_2016 at 2302.458 kb on - strand, within Pf1N1B4_2016 at 2302.529 kb on + strand, within Pf1N1B4_2016 at 2302.530 kb on - strand, within Pf1N1B4_2016 at 2302.530 kb on - strand, within Pf1N1B4_2016 at 2302.530 kb on - strand, within Pf1N1B4_2016
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 2,299,851 + -0.3 2,299,989 + Pf1N1B4_2013 0.34 +3.6 2,299,989 + Pf1N1B4_2013 0.34 +0.5 2,300,070 + Pf1N1B4_2013 0.44 +1.5 2,300,079 + Pf1N1B4_2013 0.45 +1.0 2,300,079 + Pf1N1B4_2013 0.45 -0.3 2,300,079 + Pf1N1B4_2013 0.45 +0.9 2,300,079 + Pf1N1B4_2013 0.45 +0.3 2,300,079 + Pf1N1B4_2013 0.45 -0.4 2,300,080 - Pf1N1B4_2013 0.45 -1.0 2,300,080 - Pf1N1B4_2013 0.45 +0.1 2,300,137 + Pf1N1B4_2013 0.52 +0.9 2,300,137 + Pf1N1B4_2013 0.52 +0.1 2,300,137 + Pf1N1B4_2013 0.52 -0.2 2,300,137 + Pf1N1B4_2013 0.52 +0.8 2,300,137 + +0.3 2,300,137 + Pf1N1B4_2013 0.52 +0.4 2,300,138 - Pf1N1B4_2013 0.52 -1.2 2,300,138 - Pf1N1B4_2013 0.52 -0.7 2,300,138 - Pf1N1B4_2013 0.52 +1.3 2,300,138 - Pf1N1B4_2013 0.52 +0.4 2,300,181 + Pf1N1B4_2013 0.57 +1.0 2,300,181 + Pf1N1B4_2013 0.57 +1.3 2,300,181 + Pf1N1B4_2013 0.57 -0.4 2,300,181 + Pf1N1B4_2013 0.57 -2.3 2,300,182 - Pf1N1B4_2013 0.58 -1.3 2,300,182 - Pf1N1B4_2013 0.58 +0.9 2,300,184 + Pf1N1B4_2013 0.58 -1.5 2,300,184 + Pf1N1B4_2013 0.58 +0.9 2,300,184 + Pf1N1B4_2013 0.58 +0.7 2,300,184 + Pf1N1B4_2013 0.58 -0.3 2,300,185 - Pf1N1B4_2013 0.58 +1.3 2,300,185 - Pf1N1B4_2013 0.58 +0.5 2,300,351 + Pf1N1B4_2013 0.78 -2.2 2,300,404 - Pf1N1B4_2013 0.85 -0.4 2,300,580 - -1.4 2,300,587 + -1.5 2,300,587 + -0.4 2,300,587 + -0.1 2,300,588 - -0.0 2,300,589 + -0.1 2,300,590 - -0.8 2,300,590 - +1.3 2,300,783 + Pf1N1B4_2014 0.16 -1.7 2,300,783 + Pf1N1B4_2014 0.16 +0.6 2,300,783 + Pf1N1B4_2014 0.16 -0.3 2,300,784 - Pf1N1B4_2014 0.16 -0.1 2,300,784 - Pf1N1B4_2014 0.16 -1.5 2,300,784 - Pf1N1B4_2014 0.16 -0.0 2,300,785 + Pf1N1B4_2014 0.16 -0.7 2,300,785 + Pf1N1B4_2014 0.16 -1.3 2,300,786 - Pf1N1B4_2014 0.16 -0.7 2,300,822 + Pf1N1B4_2014 0.20 -0.5 2,300,843 - Pf1N1B4_2014 0.22 -1.3 2,300,843 - Pf1N1B4_2014 0.22 -0.1 2,301,434 + Pf1N1B4_2014 0.88 -0.7 2,301,511 + +0.7 2,301,511 + -0.2 2,301,511 + +1.3 2,301,512 - +1.3 2,301,512 - -0.7 2,301,512 - +2.8 2,301,579 + -0.7 2,301,579 + -0.0 2,301,579 + +2.9 2,301,579 + -0.3 2,301,579 + +0.9 2,301,580 - -1.5 2,301,601 + +0.4 2,301,601 + -0.5 2,301,602 - -0.8 2,301,602 - +1.0 2,301,602 - +0.9 2,301,632 - +0.1 2,301,632 - -0.3 2,301,840 + Pf1N1B4_2015 0.30 -2.0 2,301,840 + Pf1N1B4_2015 0.30 -1.9 2,301,841 - Pf1N1B4_2015 0.30 -1.0 2,301,841 - Pf1N1B4_2015 0.30 +0.2 2,301,921 + Pf1N1B4_2015 0.42 +0.6 2,302,071 + Pf1N1B4_2015 0.64 -0.3 2,302,071 + Pf1N1B4_2015 0.64 +0.0 2,302,072 - Pf1N1B4_2015 0.64 +0.2 2,302,072 - Pf1N1B4_2015 0.64 +0.6 2,302,149 + Pf1N1B4_2015 0.76 +1.0 2,302,149 + Pf1N1B4_2015 0.76 +0.1 2,302,150 - Pf1N1B4_2015 0.76 -1.3 2,302,328 + +1.3 2,302,329 - -1.3 2,302,329 - +0.8 2,302,329 - +0.9 2,302,329 - +0.7 2,302,329 - -1.7 2,302,329 - -0.4 2,302,329 - -0.6 2,302,329 - -0.4 2,302,330 + +0.6 2,302,330 + +2.3 2,302,330 + +2.3 2,302,330 + +1.1 2,302,331 - +1.7 2,302,331 - +1.2 2,302,331 - +0.3 2,302,331 - -0.6 2,302,331 - -0.9 2,302,331 - +0.1 2,302,331 - -0.0 2,302,331 - -1.5 2,302,331 - -1.7 2,302,379 + Pf1N1B4_2016 0.12 -1.9 2,302,380 - Pf1N1B4_2016 0.12 -0.5 2,302,380 - Pf1N1B4_2016 0.12 +0.8 2,302,380 - Pf1N1B4_2016 0.12 -0.7 2,302,458 - Pf1N1B4_2016 0.29 -0.4 2,302,458 - Pf1N1B4_2016 0.29 +0.1 2,302,529 + Pf1N1B4_2016 0.45 -1.3 2,302,530 - Pf1N1B4_2016 0.45 +0.9 2,302,530 - Pf1N1B4_2016 0.45 -0.8 2,302,530 - Pf1N1B4_2016 0.45 -0.5
Or see this region's nucleotide sequence