Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1867 and Pf1N1B4_1868 are separated by 54 nucleotides Pf1N1B4_1868 and Pf1N1B4_1869 are separated by 37 nucleotides Pf1N1B4_1869 and Pf1N1B4_1870 overlap by 8 nucleotides Pf1N1B4_1870 and Pf1N1B4_1871 overlap by 1 nucleotides
Pf1N1B4_1867 - hypothetical protein, at 2,136,795 to 2,138,684
_1867
Pf1N1B4_1868 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 2,138,739 to 2,139,455
_1868
Pf1N1B4_1869 - Zinc ABC transporter, inner membrane permease protein ZnuB, at 2,139,493 to 2,140,281
_1869
Pf1N1B4_1870 - Zinc ABC transporter, ATP-binding protein ZnuC, at 2,140,274 to 2,141,059
_1870
Pf1N1B4_1871 - Zinc uptake regulation protein ZUR, at 2,141,059 to 2,141,463
_1871
Position (kb)
2139
2140
2141 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2138.495 kb on + strand, within Pf1N1B4_1867 at 2138.495 kb on + strand, within Pf1N1B4_1867 at 2138.495 kb on + strand, within Pf1N1B4_1867 at 2138.496 kb on - strand at 2138.541 kb on + strand at 2138.541 kb on + strand at 2138.625 kb on + strand at 2138.625 kb on + strand at 2138.625 kb on + strand at 2138.626 kb on - strand at 2138.626 kb on - strand at 2138.682 kb on + strand at 2138.683 kb on - strand at 2138.683 kb on - strand at 2138.747 kb on + strand at 2138.747 kb on + strand at 2138.747 kb on + strand at 2138.747 kb on + strand at 2138.748 kb on - strand at 2138.748 kb on - strand at 2138.866 kb on + strand, within Pf1N1B4_1868 at 2138.908 kb on + strand, within Pf1N1B4_1868 at 2138.909 kb on - strand, within Pf1N1B4_1868 at 2138.909 kb on - strand, within Pf1N1B4_1868 at 2138.909 kb on - strand, within Pf1N1B4_1868 at 2138.961 kb on - strand, within Pf1N1B4_1868 at 2138.984 kb on + strand, within Pf1N1B4_1868 at 2138.985 kb on - strand, within Pf1N1B4_1868 at 2139.211 kb on + strand, within Pf1N1B4_1868 at 2139.211 kb on + strand, within Pf1N1B4_1868 at 2139.211 kb on + strand, within Pf1N1B4_1868 at 2139.211 kb on + strand, within Pf1N1B4_1868 at 2139.211 kb on + strand, within Pf1N1B4_1868 at 2139.211 kb on + strand, within Pf1N1B4_1868 at 2139.211 kb on + strand, within Pf1N1B4_1868 at 2139.211 kb on + strand, within Pf1N1B4_1868 at 2139.211 kb on + strand, within Pf1N1B4_1868 at 2139.211 kb on + strand, within Pf1N1B4_1868 at 2139.211 kb on + strand, within Pf1N1B4_1868 at 2139.211 kb on + strand, within Pf1N1B4_1868 at 2139.211 kb on + strand, within Pf1N1B4_1868 at 2139.212 kb on - strand, within Pf1N1B4_1868 at 2139.212 kb on - strand, within Pf1N1B4_1868 at 2139.212 kb on - strand, within Pf1N1B4_1868 at 2139.212 kb on - strand, within Pf1N1B4_1868 at 2139.212 kb on - strand, within Pf1N1B4_1868 at 2139.212 kb on - strand, within Pf1N1B4_1868 at 2139.212 kb on - strand, within Pf1N1B4_1868 at 2139.212 kb on - strand, within Pf1N1B4_1868 at 2139.212 kb on - strand, within Pf1N1B4_1868 at 2139.212 kb on - strand, within Pf1N1B4_1868 at 2139.212 kb on - strand, within Pf1N1B4_1868 at 2139.212 kb on - strand, within Pf1N1B4_1868 at 2139.212 kb on - strand, within Pf1N1B4_1868 at 2139.212 kb on - strand, within Pf1N1B4_1868 at 2139.212 kb on - strand, within Pf1N1B4_1868 at 2139.212 kb on - strand, within Pf1N1B4_1868 at 2139.212 kb on - strand, within Pf1N1B4_1868 at 2139.469 kb on + strand at 2139.469 kb on + strand at 2139.495 kb on - strand at 2139.495 kb on - strand at 2139.705 kb on + strand, within Pf1N1B4_1869 at 2139.706 kb on - strand, within Pf1N1B4_1869 at 2139.706 kb on - strand, within Pf1N1B4_1869 at 2139.706 kb on - strand, within Pf1N1B4_1869 at 2139.856 kb on + strand, within Pf1N1B4_1869 at 2139.856 kb on + strand, within Pf1N1B4_1869 at 2139.862 kb on + strand, within Pf1N1B4_1869 at 2139.862 kb on + strand, within Pf1N1B4_1869 at 2139.862 kb on + strand, within Pf1N1B4_1869 at 2139.862 kb on + strand, within Pf1N1B4_1869 at 2139.862 kb on + strand, within Pf1N1B4_1869 at 2139.862 kb on + strand, within Pf1N1B4_1869 at 2139.862 kb on + strand, within Pf1N1B4_1869 at 2139.862 kb on + strand, within Pf1N1B4_1869 at 2139.862 kb on + strand, within Pf1N1B4_1869 at 2139.863 kb on - strand, within Pf1N1B4_1869 at 2139.863 kb on - strand, within Pf1N1B4_1869 at 2139.863 kb on - strand, within Pf1N1B4_1869 at 2139.863 kb on - strand, within Pf1N1B4_1869 at 2139.863 kb on - strand, within Pf1N1B4_1869 at 2139.863 kb on - strand, within Pf1N1B4_1869 at 2139.863 kb on - strand, within Pf1N1B4_1869 at 2139.865 kb on + strand, within Pf1N1B4_1869 at 2139.866 kb on - strand, within Pf1N1B4_1869 at 2139.941 kb on + strand, within Pf1N1B4_1869 at 2139.941 kb on + strand, within Pf1N1B4_1869 at 2139.942 kb on - strand, within Pf1N1B4_1869 at 2139.942 kb on - strand, within Pf1N1B4_1869 at 2139.979 kb on + strand, within Pf1N1B4_1869 at 2139.979 kb on + strand, within Pf1N1B4_1869 at 2139.979 kb on + strand, within Pf1N1B4_1869 at 2139.979 kb on + strand, within Pf1N1B4_1869 at 2139.979 kb on + strand, within Pf1N1B4_1869 at 2139.980 kb on - strand, within Pf1N1B4_1869 at 2139.980 kb on - strand, within Pf1N1B4_1869 at 2139.980 kb on - strand, within Pf1N1B4_1869 at 2139.980 kb on - strand, within Pf1N1B4_1869 at 2140.005 kb on + strand, within Pf1N1B4_1869 at 2140.006 kb on - strand, within Pf1N1B4_1869 at 2140.075 kb on + strand, within Pf1N1B4_1869 at 2140.185 kb on - strand, within Pf1N1B4_1869 at 2140.291 kb on + strand at 2140.291 kb on + strand at 2140.291 kb on + strand at 2140.291 kb on + strand at 2140.292 kb on - strand at 2140.292 kb on - strand at 2140.292 kb on - strand at 2140.292 kb on - strand at 2140.292 kb on - strand at 2140.292 kb on - strand at 2140.292 kb on - strand at 2140.292 kb on - strand at 2140.292 kb on - strand at 2140.378 kb on - strand, within Pf1N1B4_1870 at 2140.578 kb on + strand, within Pf1N1B4_1870 at 2140.579 kb on - strand, within Pf1N1B4_1870 at 2140.579 kb on - strand, within Pf1N1B4_1870 at 2140.613 kb on + strand, within Pf1N1B4_1870 at 2140.845 kb on + strand, within Pf1N1B4_1870 at 2140.957 kb on + strand, within Pf1N1B4_1870 at 2141.118 kb on + strand, within Pf1N1B4_1871 at 2141.119 kb on - strand, within Pf1N1B4_1871 at 2141.119 kb on - strand, within Pf1N1B4_1871 at 2141.205 kb on + strand, within Pf1N1B4_1871 at 2141.205 kb on + strand, within Pf1N1B4_1871 at 2141.205 kb on + strand, within Pf1N1B4_1871 at 2141.205 kb on + strand, within Pf1N1B4_1871 at 2141.206 kb on - strand, within Pf1N1B4_1871 at 2141.206 kb on - strand, within Pf1N1B4_1871 at 2141.278 kb on + strand, within Pf1N1B4_1871 at 2141.278 kb on + strand, within Pf1N1B4_1871 at 2141.278 kb on + strand, within Pf1N1B4_1871 at 2141.278 kb on + strand, within Pf1N1B4_1871 at 2141.279 kb on - strand, within Pf1N1B4_1871 at 2141.279 kb on - strand, within Pf1N1B4_1871 at 2141.279 kb on - strand, within Pf1N1B4_1871 at 2141.279 kb on - strand, within Pf1N1B4_1871 at 2141.279 kb on - strand, within Pf1N1B4_1871
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 2,138,495 + Pf1N1B4_1867 0.90 -2.2 2,138,495 + Pf1N1B4_1867 0.90 -0.9 2,138,495 + Pf1N1B4_1867 0.90 -0.7 2,138,496 - +0.9 2,138,541 + +1.0 2,138,541 + -1.2 2,138,625 + +0.3 2,138,625 + -1.0 2,138,625 + -0.0 2,138,626 - +0.3 2,138,626 - +1.3 2,138,682 + -0.0 2,138,683 - +0.1 2,138,683 - +0.5 2,138,747 + +2.0 2,138,747 + -0.0 2,138,747 + +0.6 2,138,747 + +0.9 2,138,748 - -0.5 2,138,748 - -0.3 2,138,866 + Pf1N1B4_1868 0.18 +0.2 2,138,908 + Pf1N1B4_1868 0.24 -0.3 2,138,909 - Pf1N1B4_1868 0.24 +0.1 2,138,909 - Pf1N1B4_1868 0.24 +0.1 2,138,909 - Pf1N1B4_1868 0.24 -1.4 2,138,961 - Pf1N1B4_1868 0.31 +0.3 2,138,984 + Pf1N1B4_1868 0.34 +0.8 2,138,985 - Pf1N1B4_1868 0.34 -0.8 2,139,211 + Pf1N1B4_1868 0.66 +1.7 2,139,211 + Pf1N1B4_1868 0.66 -0.5 2,139,211 + Pf1N1B4_1868 0.66 -2.0 2,139,211 + Pf1N1B4_1868 0.66 +0.2 2,139,211 + Pf1N1B4_1868 0.66 -0.3 2,139,211 + Pf1N1B4_1868 0.66 -0.8 2,139,211 + Pf1N1B4_1868 0.66 +0.4 2,139,211 + Pf1N1B4_1868 0.66 -1.2 2,139,211 + Pf1N1B4_1868 0.66 -2.9 2,139,211 + Pf1N1B4_1868 0.66 -0.9 2,139,211 + Pf1N1B4_1868 0.66 +1.0 2,139,211 + Pf1N1B4_1868 0.66 -0.5 2,139,211 + Pf1N1B4_1868 0.66 -1.5 2,139,212 - Pf1N1B4_1868 0.66 +0.3 2,139,212 - Pf1N1B4_1868 0.66 +1.5 2,139,212 - Pf1N1B4_1868 0.66 -1.9 2,139,212 - Pf1N1B4_1868 0.66 -0.4 2,139,212 - Pf1N1B4_1868 0.66 +0.9 2,139,212 - Pf1N1B4_1868 0.66 -0.6 2,139,212 - Pf1N1B4_1868 0.66 +0.2 2,139,212 - Pf1N1B4_1868 0.66 -0.3 2,139,212 - Pf1N1B4_1868 0.66 -1.3 2,139,212 - Pf1N1B4_1868 0.66 +1.3 2,139,212 - Pf1N1B4_1868 0.66 +0.1 2,139,212 - Pf1N1B4_1868 0.66 -0.9 2,139,212 - Pf1N1B4_1868 0.66 -0.1 2,139,212 - Pf1N1B4_1868 0.66 -1.3 2,139,212 - Pf1N1B4_1868 0.66 +0.3 2,139,212 - Pf1N1B4_1868 0.66 -1.0 2,139,212 - Pf1N1B4_1868 0.66 -0.1 2,139,469 + +1.5 2,139,469 + +0.5 2,139,495 - +1.3 2,139,495 - -0.1 2,139,705 + Pf1N1B4_1869 0.27 -0.3 2,139,706 - Pf1N1B4_1869 0.27 +0.8 2,139,706 - Pf1N1B4_1869 0.27 -1.5 2,139,706 - Pf1N1B4_1869 0.27 -0.7 2,139,856 + Pf1N1B4_1869 0.46 -3.3 2,139,856 + Pf1N1B4_1869 0.46 +0.3 2,139,862 + Pf1N1B4_1869 0.47 -0.3 2,139,862 + Pf1N1B4_1869 0.47 -2.2 2,139,862 + Pf1N1B4_1869 0.47 -0.1 2,139,862 + Pf1N1B4_1869 0.47 -0.3 2,139,862 + Pf1N1B4_1869 0.47 -0.5 2,139,862 + Pf1N1B4_1869 0.47 +0.8 2,139,862 + Pf1N1B4_1869 0.47 -1.0 2,139,862 + Pf1N1B4_1869 0.47 -1.5 2,139,862 + Pf1N1B4_1869 0.47 -0.8 2,139,863 - Pf1N1B4_1869 0.47 +0.7 2,139,863 - Pf1N1B4_1869 0.47 -1.5 2,139,863 - Pf1N1B4_1869 0.47 -1.7 2,139,863 - Pf1N1B4_1869 0.47 -0.0 2,139,863 - Pf1N1B4_1869 0.47 -1.0 2,139,863 - Pf1N1B4_1869 0.47 +0.1 2,139,863 - Pf1N1B4_1869 0.47 -1.7 2,139,865 + Pf1N1B4_1869 0.47 -3.0 2,139,866 - Pf1N1B4_1869 0.47 -1.3 2,139,941 + Pf1N1B4_1869 0.57 +0.3 2,139,941 + Pf1N1B4_1869 0.57 -0.5 2,139,942 - Pf1N1B4_1869 0.57 -1.2 2,139,942 - Pf1N1B4_1869 0.57 -0.5 2,139,979 + Pf1N1B4_1869 0.62 -1.4 2,139,979 + Pf1N1B4_1869 0.62 -0.7 2,139,979 + Pf1N1B4_1869 0.62 +0.1 2,139,979 + Pf1N1B4_1869 0.62 +1.3 2,139,979 + Pf1N1B4_1869 0.62 -2.0 2,139,980 - Pf1N1B4_1869 0.62 -0.3 2,139,980 - Pf1N1B4_1869 0.62 -0.7 2,139,980 - Pf1N1B4_1869 0.62 -0.7 2,139,980 - Pf1N1B4_1869 0.62 -1.1 2,140,005 + Pf1N1B4_1869 0.65 -1.5 2,140,006 - Pf1N1B4_1869 0.65 +0.7 2,140,075 + Pf1N1B4_1869 0.74 -0.9 2,140,185 - Pf1N1B4_1869 0.88 -0.9 2,140,291 + +0.9 2,140,291 + -1.0 2,140,291 + +0.9 2,140,291 + -1.9 2,140,292 - +0.9 2,140,292 - -0.0 2,140,292 - +0.5 2,140,292 - -0.7 2,140,292 - -1.8 2,140,292 - +0.3 2,140,292 - -0.5 2,140,292 - -1.0 2,140,292 - +0.5 2,140,378 - Pf1N1B4_1870 0.13 +1.7 2,140,578 + Pf1N1B4_1870 0.39 -0.3 2,140,579 - Pf1N1B4_1870 0.39 -0.6 2,140,579 - Pf1N1B4_1870 0.39 -0.7 2,140,613 + Pf1N1B4_1870 0.43 -1.9 2,140,845 + Pf1N1B4_1870 0.73 -1.3 2,140,957 + Pf1N1B4_1870 0.87 -0.5 2,141,118 + Pf1N1B4_1871 0.15 -0.8 2,141,119 - Pf1N1B4_1871 0.15 -0.3 2,141,119 - Pf1N1B4_1871 0.15 -2.4 2,141,205 + Pf1N1B4_1871 0.36 +2.3 2,141,205 + Pf1N1B4_1871 0.36 -0.5 2,141,205 + Pf1N1B4_1871 0.36 -0.3 2,141,205 + Pf1N1B4_1871 0.36 -1.5 2,141,206 - Pf1N1B4_1871 0.36 +0.2 2,141,206 - Pf1N1B4_1871 0.36 +0.7 2,141,278 + Pf1N1B4_1871 0.54 -0.5 2,141,278 + Pf1N1B4_1871 0.54 -0.4 2,141,278 + Pf1N1B4_1871 0.54 -0.5 2,141,278 + Pf1N1B4_1871 0.54 +0.5 2,141,279 - Pf1N1B4_1871 0.54 +0.1 2,141,279 - Pf1N1B4_1871 0.54 -0.9 2,141,279 - Pf1N1B4_1871 0.54 -2.8 2,141,279 - Pf1N1B4_1871 0.54 -0.1 2,141,279 - Pf1N1B4_1871 0.54 -0.1
Or see this region's nucleotide sequence