Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1580 and Pf1N1B4_1581 are separated by 151 nucleotides Pf1N1B4_1581 and Pf1N1B4_1582 are separated by 116 nucleotides
Pf1N1B4_1580 - Histidine transport protein (permease), at 1,819,399 to 1,820,808
_1580
Pf1N1B4_1581 - Histidine ammonia-lyase (EC 4.3.1.3), at 1,820,960 to 1,822,492
_1581
Pf1N1B4_1582 - Histidine ammonia-lyase (EC 4.3.1.3) (from data) , at 1,822,609 to 1,824,132
_1582
Position (kb)
1820
1821
1822
1823 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1820.060 kb on + strand, within Pf1N1B4_1580 at 1820.060 kb on + strand, within Pf1N1B4_1580 at 1820.060 kb on + strand, within Pf1N1B4_1580 at 1820.061 kb on - strand, within Pf1N1B4_1580 at 1820.061 kb on - strand, within Pf1N1B4_1580 at 1820.061 kb on - strand, within Pf1N1B4_1580 at 1820.061 kb on - strand, within Pf1N1B4_1580 at 1820.061 kb on - strand, within Pf1N1B4_1580 at 1820.073 kb on + strand, within Pf1N1B4_1580 at 1820.073 kb on + strand, within Pf1N1B4_1580 at 1820.073 kb on + strand, within Pf1N1B4_1580 at 1820.073 kb on + strand, within Pf1N1B4_1580 at 1820.073 kb on + strand, within Pf1N1B4_1580 at 1820.146 kb on + strand, within Pf1N1B4_1580 at 1820.386 kb on - strand, within Pf1N1B4_1580 at 1820.386 kb on - strand, within Pf1N1B4_1580 at 1820.468 kb on + strand, within Pf1N1B4_1580 at 1820.468 kb on + strand, within Pf1N1B4_1580 at 1820.468 kb on + strand, within Pf1N1B4_1580 at 1820.468 kb on + strand, within Pf1N1B4_1580 at 1820.468 kb on + strand, within Pf1N1B4_1580 at 1820.468 kb on + strand, within Pf1N1B4_1580 at 1820.468 kb on + strand, within Pf1N1B4_1580 at 1820.468 kb on + strand, within Pf1N1B4_1580 at 1820.468 kb on + strand, within Pf1N1B4_1580 at 1820.468 kb on + strand, within Pf1N1B4_1580 at 1820.468 kb on + strand, within Pf1N1B4_1580 at 1820.468 kb on + strand, within Pf1N1B4_1580 at 1820.468 kb on + strand, within Pf1N1B4_1580 at 1820.468 kb on + strand, within Pf1N1B4_1580 at 1820.468 kb on + strand, within Pf1N1B4_1580 at 1820.469 kb on - strand, within Pf1N1B4_1580 at 1820.469 kb on - strand, within Pf1N1B4_1580 at 1820.469 kb on - strand at 1820.469 kb on - strand, within Pf1N1B4_1580 at 1820.469 kb on - strand, within Pf1N1B4_1580 at 1820.523 kb on - strand, within Pf1N1B4_1580 at 1820.557 kb on + strand, within Pf1N1B4_1580 at 1820.557 kb on + strand, within Pf1N1B4_1580 at 1820.558 kb on - strand, within Pf1N1B4_1580 at 1820.558 kb on - strand, within Pf1N1B4_1580 at 1820.558 kb on - strand, within Pf1N1B4_1580 at 1820.561 kb on + strand, within Pf1N1B4_1580 at 1820.561 kb on + strand, within Pf1N1B4_1580 at 1820.561 kb on + strand, within Pf1N1B4_1580 at 1820.561 kb on + strand, within Pf1N1B4_1580 at 1820.561 kb on + strand, within Pf1N1B4_1580 at 1820.561 kb on + strand, within Pf1N1B4_1580 at 1820.562 kb on - strand, within Pf1N1B4_1580 at 1820.562 kb on - strand, within Pf1N1B4_1580 at 1820.562 kb on - strand, within Pf1N1B4_1580 at 1820.562 kb on - strand, within Pf1N1B4_1580 at 1820.562 kb on - strand, within Pf1N1B4_1580 at 1820.562 kb on - strand, within Pf1N1B4_1580 at 1820.663 kb on + strand, within Pf1N1B4_1580 at 1820.663 kb on + strand, within Pf1N1B4_1580 at 1820.663 kb on + strand, within Pf1N1B4_1580 at 1820.663 kb on + strand, within Pf1N1B4_1580 at 1820.664 kb on - strand, within Pf1N1B4_1580 at 1820.714 kb on + strand at 1820.714 kb on + strand at 1820.715 kb on - strand at 1820.715 kb on - strand at 1820.715 kb on - strand at 1820.760 kb on + strand at 1820.760 kb on + strand at 1820.760 kb on + strand at 1820.760 kb on + strand at 1820.761 kb on - strand at 1820.819 kb on + strand at 1820.819 kb on + strand at 1820.824 kb on - strand at 1820.824 kb on - strand at 1821.066 kb on + strand at 1821.066 kb on + strand at 1821.066 kb on + strand at 1821.067 kb on - strand at 1821.067 kb on - strand at 1821.067 kb on - strand at 1821.067 kb on - strand at 1821.508 kb on + strand, within Pf1N1B4_1581 at 1821.509 kb on - strand, within Pf1N1B4_1581 at 1821.509 kb on - strand, within Pf1N1B4_1581 at 1821.882 kb on + strand, within Pf1N1B4_1581 at 1821.882 kb on + strand, within Pf1N1B4_1581 at 1821.882 kb on + strand, within Pf1N1B4_1581 at 1821.883 kb on - strand, within Pf1N1B4_1581 at 1822.000 kb on - strand, within Pf1N1B4_1581 at 1822.009 kb on + strand, within Pf1N1B4_1581 at 1822.010 kb on - strand, within Pf1N1B4_1581 at 1822.435 kb on - strand at 1822.496 kb on + strand at 1822.521 kb on + strand at 1822.522 kb on - strand at 1822.522 kb on - strand at 1822.522 kb on - strand at 1822.597 kb on + strand at 1822.598 kb on - strand at 1822.598 kb on - strand at 1822.604 kb on - strand at 1822.604 kb on - strand at 1822.743 kb on + strand at 1822.805 kb on + strand, within Pf1N1B4_1582 at 1822.806 kb on - strand, within Pf1N1B4_1582 at 1822.806 kb on - strand, within Pf1N1B4_1582 at 1822.905 kb on + strand, within Pf1N1B4_1582 at 1822.905 kb on + strand, within Pf1N1B4_1582 at 1822.905 kb on + strand, within Pf1N1B4_1582 at 1822.905 kb on + strand, within Pf1N1B4_1582 at 1822.905 kb on + strand, within Pf1N1B4_1582 at 1822.906 kb on - strand, within Pf1N1B4_1582 at 1822.906 kb on - strand, within Pf1N1B4_1582 at 1822.906 kb on - strand, within Pf1N1B4_1582 at 1822.906 kb on - strand, within Pf1N1B4_1582 at 1822.910 kb on + strand, within Pf1N1B4_1582 at 1822.910 kb on + strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.973 kb on + strand, within Pf1N1B4_1582 at 1822.973 kb on + strand, within Pf1N1B4_1582 at 1822.974 kb on - strand, within Pf1N1B4_1582 at 1822.974 kb on - strand, within Pf1N1B4_1582 at 1822.974 kb on - strand, within Pf1N1B4_1582 at 1822.974 kb on - strand, within Pf1N1B4_1582 at 1822.974 kb on - strand, within Pf1N1B4_1582 at 1822.974 kb on - strand, within Pf1N1B4_1582 at 1822.974 kb on - strand, within Pf1N1B4_1582
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 1,820,060 + Pf1N1B4_1580 0.47 +1.1 1,820,060 + Pf1N1B4_1580 0.47 +0.5 1,820,060 + Pf1N1B4_1580 0.47 -0.9 1,820,061 - Pf1N1B4_1580 0.47 +0.4 1,820,061 - Pf1N1B4_1580 0.47 -0.5 1,820,061 - Pf1N1B4_1580 0.47 +0.2 1,820,061 - Pf1N1B4_1580 0.47 +2.4 1,820,061 - Pf1N1B4_1580 0.47 -0.2 1,820,073 + Pf1N1B4_1580 0.48 -0.9 1,820,073 + Pf1N1B4_1580 0.48 -0.3 1,820,073 + Pf1N1B4_1580 0.48 +0.7 1,820,073 + Pf1N1B4_1580 0.48 +0.2 1,820,073 + Pf1N1B4_1580 0.48 -0.9 1,820,146 + Pf1N1B4_1580 0.53 +0.8 1,820,386 - Pf1N1B4_1580 0.70 -0.5 1,820,386 - Pf1N1B4_1580 0.70 +1.1 1,820,468 + Pf1N1B4_1580 0.76 +1.1 1,820,468 + Pf1N1B4_1580 0.76 -0.5 1,820,468 + Pf1N1B4_1580 0.76 +1.6 1,820,468 + Pf1N1B4_1580 0.76 +0.4 1,820,468 + Pf1N1B4_1580 0.76 +1.0 1,820,468 + Pf1N1B4_1580 0.76 -0.9 1,820,468 + Pf1N1B4_1580 0.76 -1.7 1,820,468 + Pf1N1B4_1580 0.76 +0.4 1,820,468 + Pf1N1B4_1580 0.76 +1.7 1,820,468 + Pf1N1B4_1580 0.76 +0.1 1,820,468 + Pf1N1B4_1580 0.76 +0.7 1,820,468 + Pf1N1B4_1580 0.76 +0.5 1,820,468 + Pf1N1B4_1580 0.76 +1.4 1,820,468 + Pf1N1B4_1580 0.76 +1.1 1,820,468 + Pf1N1B4_1580 0.76 +0.5 1,820,469 - Pf1N1B4_1580 0.76 -1.7 1,820,469 - Pf1N1B4_1580 0.76 +0.7 1,820,469 - +2.7 1,820,469 - Pf1N1B4_1580 0.76 +1.1 1,820,469 - Pf1N1B4_1580 0.76 +0.4 1,820,523 - Pf1N1B4_1580 0.80 +1.1 1,820,557 + Pf1N1B4_1580 0.82 -2.2 1,820,557 + Pf1N1B4_1580 0.82 +0.8 1,820,558 - Pf1N1B4_1580 0.82 -2.3 1,820,558 - Pf1N1B4_1580 0.82 +0.4 1,820,558 - Pf1N1B4_1580 0.82 -1.5 1,820,561 + Pf1N1B4_1580 0.82 +1.1 1,820,561 + Pf1N1B4_1580 0.82 -0.7 1,820,561 + Pf1N1B4_1580 0.82 -0.3 1,820,561 + Pf1N1B4_1580 0.82 -0.1 1,820,561 + Pf1N1B4_1580 0.82 -2.2 1,820,561 + Pf1N1B4_1580 0.82 +0.3 1,820,562 - Pf1N1B4_1580 0.82 +1.9 1,820,562 - Pf1N1B4_1580 0.82 -1.2 1,820,562 - Pf1N1B4_1580 0.82 +0.5 1,820,562 - Pf1N1B4_1580 0.82 +0.2 1,820,562 - Pf1N1B4_1580 0.82 -0.2 1,820,562 - Pf1N1B4_1580 0.82 +1.1 1,820,663 + Pf1N1B4_1580 0.90 -1.1 1,820,663 + Pf1N1B4_1580 0.90 +0.8 1,820,663 + Pf1N1B4_1580 0.90 -2.0 1,820,663 + Pf1N1B4_1580 0.90 -0.2 1,820,664 - Pf1N1B4_1580 0.90 -0.1 1,820,714 + -1.0 1,820,714 + -0.5 1,820,715 - +0.6 1,820,715 - -0.7 1,820,715 - -1.9 1,820,760 + +1.9 1,820,760 + -0.1 1,820,760 + +0.4 1,820,760 + +3.3 1,820,761 - -0.0 1,820,819 + +0.1 1,820,819 + +0.4 1,820,824 - -3.2 1,820,824 - +0.1 1,821,066 + -1.7 1,821,066 + +0.3 1,821,066 + -1.3 1,821,067 - +0.1 1,821,067 - +0.1 1,821,067 - -2.5 1,821,067 - -2.8 1,821,508 + Pf1N1B4_1581 0.36 +0.5 1,821,509 - Pf1N1B4_1581 0.36 -2.7 1,821,509 - Pf1N1B4_1581 0.36 -2.0 1,821,882 + Pf1N1B4_1581 0.60 -0.0 1,821,882 + Pf1N1B4_1581 0.60 -1.6 1,821,882 + Pf1N1B4_1581 0.60 +0.7 1,821,883 - Pf1N1B4_1581 0.60 -2.9 1,822,000 - Pf1N1B4_1581 0.68 -1.8 1,822,009 + Pf1N1B4_1581 0.68 -0.9 1,822,010 - Pf1N1B4_1581 0.68 -1.0 1,822,435 - -0.5 1,822,496 + -3.0 1,822,521 + +1.8 1,822,522 - +0.3 1,822,522 - +2.3 1,822,522 - -0.6 1,822,597 + -0.2 1,822,598 - -0.5 1,822,598 - -0.6 1,822,604 - +0.3 1,822,604 - -0.1 1,822,743 + -0.7 1,822,805 + Pf1N1B4_1582 0.13 -0.1 1,822,806 - Pf1N1B4_1582 0.13 -2.0 1,822,806 - Pf1N1B4_1582 0.13 -1.3 1,822,905 + Pf1N1B4_1582 0.19 +0.1 1,822,905 + Pf1N1B4_1582 0.19 -0.7 1,822,905 + Pf1N1B4_1582 0.19 -0.2 1,822,905 + Pf1N1B4_1582 0.19 +1.7 1,822,905 + Pf1N1B4_1582 0.19 -1.6 1,822,906 - Pf1N1B4_1582 0.19 +0.9 1,822,906 - Pf1N1B4_1582 0.19 -0.4 1,822,906 - Pf1N1B4_1582 0.19 +0.1 1,822,906 - Pf1N1B4_1582 0.19 -0.2 1,822,910 + Pf1N1B4_1582 0.20 +0.1 1,822,910 + Pf1N1B4_1582 0.20 +0.0 1,822,911 - Pf1N1B4_1582 0.20 +0.1 1,822,911 - Pf1N1B4_1582 0.20 +0.5 1,822,911 - Pf1N1B4_1582 0.20 +0.1 1,822,911 - Pf1N1B4_1582 0.20 +0.8 1,822,911 - Pf1N1B4_1582 0.20 -0.9 1,822,911 - Pf1N1B4_1582 0.20 -0.6 1,822,911 - Pf1N1B4_1582 0.20 +0.1 1,822,911 - Pf1N1B4_1582 0.20 +0.9 1,822,911 - Pf1N1B4_1582 0.20 +0.7 1,822,911 - Pf1N1B4_1582 0.20 +0.3 1,822,911 - Pf1N1B4_1582 0.20 -1.3 1,822,911 - Pf1N1B4_1582 0.20 +1.7 1,822,911 - Pf1N1B4_1582 0.20 +0.8 1,822,973 + Pf1N1B4_1582 0.24 -0.4 1,822,973 + Pf1N1B4_1582 0.24 +0.5 1,822,974 - Pf1N1B4_1582 0.24 +0.1 1,822,974 - Pf1N1B4_1582 0.24 +1.9 1,822,974 - Pf1N1B4_1582 0.24 +0.1 1,822,974 - Pf1N1B4_1582 0.24 -2.0 1,822,974 - Pf1N1B4_1582 0.24 +0.7 1,822,974 - Pf1N1B4_1582 0.24 +0.5 1,822,974 - Pf1N1B4_1582 0.24 -1.3
Or see this region's nucleotide sequence