Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1286 and Pf1N1B4_1287 are separated by 307 nucleotides Pf1N1B4_1287 and Pf1N1B4_1288 are separated by 53 nucleotides Pf1N1B4_1288 and Pf1N1B4_1289 are separated by 12 nucleotides
Pf1N1B4_1286 - Probable transmembrane protein, at 1,467,634 to 1,469,559
_1286
Pf1N1B4_1287 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 1,469,867 to 1,470,424
_1287
Pf1N1B4_1288 - lipoprotein, putative, at 1,470,478 to 1,471,095
_1288
Pf1N1B4_1289 - 3-demethylubiquinone-9 3-methyltransferase (EC 2.1.1.64), at 1,471,108 to 1,471,857
_1289
Position (kb)
1469
1470
1471 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1468.945 kb on + strand at 1468.945 kb on + strand, within Pf1N1B4_1286 at 1468.945 kb on + strand, within Pf1N1B4_1286 at 1468.945 kb on + strand, within Pf1N1B4_1286 at 1468.946 kb on - strand, within Pf1N1B4_1286 at 1468.946 kb on - strand, within Pf1N1B4_1286 at 1468.946 kb on - strand, within Pf1N1B4_1286 at 1469.103 kb on + strand, within Pf1N1B4_1286 at 1469.284 kb on - strand, within Pf1N1B4_1286 at 1469.299 kb on - strand, within Pf1N1B4_1286 at 1469.393 kb on + strand at 1469.492 kb on + strand at 1469.492 kb on + strand at 1469.492 kb on + strand at 1469.650 kb on + strand at 1469.650 kb on + strand at 1469.674 kb on + strand at 1469.674 kb on + strand at 1469.675 kb on - strand at 1469.745 kb on + strand at 1469.745 kb on + strand at 1469.745 kb on + strand at 1469.746 kb on - strand at 1469.746 kb on - strand at 1469.746 kb on - strand at 1469.746 kb on - strand at 1469.857 kb on - strand at 1469.857 kb on - strand at 1469.881 kb on - strand at 1469.881 kb on - strand at 1469.917 kb on + strand at 1469.918 kb on - strand at 1469.923 kb on + strand, within Pf1N1B4_1287 at 1469.924 kb on - strand, within Pf1N1B4_1287 at 1469.950 kb on + strand, within Pf1N1B4_1287 at 1469.951 kb on - strand, within Pf1N1B4_1287 at 1470.033 kb on + strand, within Pf1N1B4_1287 at 1470.033 kb on + strand at 1470.033 kb on + strand, within Pf1N1B4_1287 at 1470.033 kb on + strand, within Pf1N1B4_1287 at 1470.034 kb on - strand, within Pf1N1B4_1287 at 1470.057 kb on + strand, within Pf1N1B4_1287 at 1470.057 kb on + strand, within Pf1N1B4_1287 at 1470.057 kb on + strand, within Pf1N1B4_1287 at 1470.057 kb on + strand, within Pf1N1B4_1287 at 1470.058 kb on - strand, within Pf1N1B4_1287 at 1470.058 kb on - strand, within Pf1N1B4_1287 at 1470.058 kb on - strand, within Pf1N1B4_1287 at 1470.108 kb on + strand, within Pf1N1B4_1287 at 1470.316 kb on + strand, within Pf1N1B4_1287 at 1470.316 kb on + strand, within Pf1N1B4_1287 at 1470.316 kb on + strand, within Pf1N1B4_1287 at 1470.316 kb on + strand, within Pf1N1B4_1287 at 1470.317 kb on - strand, within Pf1N1B4_1287 at 1470.317 kb on - strand, within Pf1N1B4_1287 at 1470.438 kb on + strand at 1470.479 kb on + strand at 1470.479 kb on + strand at 1470.480 kb on - strand at 1470.480 kb on - strand at 1470.480 kb on - strand at 1470.480 kb on - strand at 1470.483 kb on + strand at 1470.483 kb on + strand at 1470.483 kb on + strand at 1470.483 kb on + strand at 1470.484 kb on - strand at 1470.484 kb on - strand at 1470.484 kb on - strand at 1470.484 kb on - strand at 1470.484 kb on - strand at 1470.637 kb on + strand, within Pf1N1B4_1288 at 1470.638 kb on - strand, within Pf1N1B4_1288 at 1470.638 kb on - strand, within Pf1N1B4_1288 at 1470.644 kb on + strand, within Pf1N1B4_1288 at 1470.644 kb on + strand, within Pf1N1B4_1288 at 1470.644 kb on + strand, within Pf1N1B4_1288 at 1470.645 kb on - strand, within Pf1N1B4_1288 at 1470.671 kb on + strand, within Pf1N1B4_1288 at 1470.671 kb on + strand, within Pf1N1B4_1288 at 1470.671 kb on + strand, within Pf1N1B4_1288 at 1470.671 kb on + strand, within Pf1N1B4_1288 at 1470.671 kb on + strand, within Pf1N1B4_1288 at 1470.672 kb on - strand, within Pf1N1B4_1288 at 1470.672 kb on - strand, within Pf1N1B4_1288 at 1470.672 kb on - strand, within Pf1N1B4_1288 at 1470.672 kb on - strand, within Pf1N1B4_1288 at 1470.672 kb on - strand, within Pf1N1B4_1288 at 1470.672 kb on - strand, within Pf1N1B4_1288 at 1470.672 kb on - strand, within Pf1N1B4_1288 at 1470.701 kb on + strand, within Pf1N1B4_1288 at 1471.028 kb on + strand, within Pf1N1B4_1288 at 1471.028 kb on + strand, within Pf1N1B4_1288 at 1471.029 kb on - strand, within Pf1N1B4_1288 at 1471.029 kb on - strand, within Pf1N1B4_1288 at 1471.029 kb on - strand, within Pf1N1B4_1288 at 1471.029 kb on - strand, within Pf1N1B4_1288 at 1471.202 kb on - strand, within Pf1N1B4_1289 at 1471.235 kb on + strand, within Pf1N1B4_1289 at 1471.235 kb on + strand, within Pf1N1B4_1289 at 1471.235 kb on + strand, within Pf1N1B4_1289 at 1471.235 kb on + strand, within Pf1N1B4_1289 at 1471.236 kb on - strand, within Pf1N1B4_1289 at 1471.236 kb on - strand, within Pf1N1B4_1289 at 1471.236 kb on - strand, within Pf1N1B4_1289 at 1471.236 kb on - strand, within Pf1N1B4_1289 at 1471.236 kb on - strand, within Pf1N1B4_1289 at 1471.236 kb on - strand, within Pf1N1B4_1289 at 1471.236 kb on - strand, within Pf1N1B4_1289 at 1471.236 kb on - strand, within Pf1N1B4_1289 at 1471.236 kb on - strand, within Pf1N1B4_1289 at 1471.236 kb on - strand, within Pf1N1B4_1289 at 1471.241 kb on + strand, within Pf1N1B4_1289 at 1471.404 kb on - strand, within Pf1N1B4_1289 at 1471.424 kb on + strand, within Pf1N1B4_1289 at 1471.424 kb on + strand, within Pf1N1B4_1289
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 1,468,945 + +0.0 1,468,945 + Pf1N1B4_1286 0.68 -1.0 1,468,945 + Pf1N1B4_1286 0.68 -0.3 1,468,945 + Pf1N1B4_1286 0.68 -1.6 1,468,946 - Pf1N1B4_1286 0.68 -2.4 1,468,946 - Pf1N1B4_1286 0.68 -0.3 1,468,946 - Pf1N1B4_1286 0.68 +0.4 1,469,103 + Pf1N1B4_1286 0.76 +2.3 1,469,284 - Pf1N1B4_1286 0.86 -1.8 1,469,299 - Pf1N1B4_1286 0.86 +0.4 1,469,393 + +0.1 1,469,492 + -2.7 1,469,492 + +0.1 1,469,492 + +0.7 1,469,650 + -0.2 1,469,650 + +1.0 1,469,674 + +1.0 1,469,674 + -0.0 1,469,675 - -0.4 1,469,745 + -0.7 1,469,745 + +1.6 1,469,745 + -0.3 1,469,746 - -1.0 1,469,746 - -0.3 1,469,746 - +1.6 1,469,746 - +0.3 1,469,857 - -0.7 1,469,857 - -0.6 1,469,881 - +0.8 1,469,881 - +1.0 1,469,917 + +2.0 1,469,918 - -1.6 1,469,923 + Pf1N1B4_1287 0.10 +0.0 1,469,924 - Pf1N1B4_1287 0.10 +0.4 1,469,950 + Pf1N1B4_1287 0.15 +0.0 1,469,951 - Pf1N1B4_1287 0.15 -0.6 1,470,033 + Pf1N1B4_1287 0.30 -0.4 1,470,033 + +2.6 1,470,033 + Pf1N1B4_1287 0.30 -0.0 1,470,033 + Pf1N1B4_1287 0.30 -1.3 1,470,034 - Pf1N1B4_1287 0.30 -0.8 1,470,057 + Pf1N1B4_1287 0.34 -0.3 1,470,057 + Pf1N1B4_1287 0.34 +0.0 1,470,057 + Pf1N1B4_1287 0.34 -1.3 1,470,057 + Pf1N1B4_1287 0.34 -2.0 1,470,058 - Pf1N1B4_1287 0.34 -0.4 1,470,058 - Pf1N1B4_1287 0.34 -1.3 1,470,058 - Pf1N1B4_1287 0.34 -1.0 1,470,108 + Pf1N1B4_1287 0.43 +0.2 1,470,316 + Pf1N1B4_1287 0.80 -1.6 1,470,316 + Pf1N1B4_1287 0.80 +1.0 1,470,316 + Pf1N1B4_1287 0.80 -0.9 1,470,316 + Pf1N1B4_1287 0.80 -0.6 1,470,317 - Pf1N1B4_1287 0.81 -0.1 1,470,317 - Pf1N1B4_1287 0.81 -1.3 1,470,438 + +1.0 1,470,479 + +0.8 1,470,479 + +0.3 1,470,480 - -0.9 1,470,480 - +0.0 1,470,480 - +0.6 1,470,480 - +1.0 1,470,483 + +1.0 1,470,483 + +1.6 1,470,483 + -0.6 1,470,483 + -0.6 1,470,484 - +0.6 1,470,484 - +1.1 1,470,484 - -0.3 1,470,484 - -0.3 1,470,484 - +0.7 1,470,637 + Pf1N1B4_1288 0.26 +0.5 1,470,638 - Pf1N1B4_1288 0.26 +0.9 1,470,638 - Pf1N1B4_1288 0.26 -0.2 1,470,644 + Pf1N1B4_1288 0.27 -1.0 1,470,644 + Pf1N1B4_1288 0.27 -2.0 1,470,644 + Pf1N1B4_1288 0.27 -0.1 1,470,645 - Pf1N1B4_1288 0.27 +0.4 1,470,671 + Pf1N1B4_1288 0.31 +0.4 1,470,671 + Pf1N1B4_1288 0.31 +0.4 1,470,671 + Pf1N1B4_1288 0.31 -0.6 1,470,671 + Pf1N1B4_1288 0.31 +0.0 1,470,671 + Pf1N1B4_1288 0.31 +0.4 1,470,672 - Pf1N1B4_1288 0.31 +1.6 1,470,672 - Pf1N1B4_1288 0.31 -1.1 1,470,672 - Pf1N1B4_1288 0.31 -0.6 1,470,672 - Pf1N1B4_1288 0.31 -2.7 1,470,672 - Pf1N1B4_1288 0.31 +0.1 1,470,672 - Pf1N1B4_1288 0.31 +1.2 1,470,672 - Pf1N1B4_1288 0.31 -0.4 1,470,701 + Pf1N1B4_1288 0.36 +0.6 1,471,028 + Pf1N1B4_1288 0.89 -0.9 1,471,028 + Pf1N1B4_1288 0.89 -0.8 1,471,029 - Pf1N1B4_1288 0.89 +0.6 1,471,029 - Pf1N1B4_1288 0.89 +1.4 1,471,029 - Pf1N1B4_1288 0.89 -0.1 1,471,029 - Pf1N1B4_1288 0.89 -0.8 1,471,202 - Pf1N1B4_1289 0.13 +0.0 1,471,235 + Pf1N1B4_1289 0.17 -0.6 1,471,235 + Pf1N1B4_1289 0.17 -1.6 1,471,235 + Pf1N1B4_1289 0.17 -2.0 1,471,235 + Pf1N1B4_1289 0.17 -2.7 1,471,236 - Pf1N1B4_1289 0.17 +0.0 1,471,236 - Pf1N1B4_1289 0.17 +0.5 1,471,236 - Pf1N1B4_1289 0.17 +0.4 1,471,236 - Pf1N1B4_1289 0.17 -1.0 1,471,236 - Pf1N1B4_1289 0.17 -0.2 1,471,236 - Pf1N1B4_1289 0.17 +2.3 1,471,236 - Pf1N1B4_1289 0.17 -0.9 1,471,236 - Pf1N1B4_1289 0.17 -0.8 1,471,236 - Pf1N1B4_1289 0.17 -0.7 1,471,236 - Pf1N1B4_1289 0.17 +1.5 1,471,241 + Pf1N1B4_1289 0.18 -0.3 1,471,404 - Pf1N1B4_1289 0.39 +0.0 1,471,424 + Pf1N1B4_1289 0.42 +0.3 1,471,424 + Pf1N1B4_1289 0.42 +0.6
Or see this region's nucleotide sequence