Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_122 and Pf1N1B4_123 are separated by 9 nucleotides Pf1N1B4_123 and Pf1N1B4_124 are separated by 245 nucleotides Pf1N1B4_124 and Pf1N1B4_125 are separated by 189 nucleotides Pf1N1B4_125 and Pf1N1B4_126 are separated by 127 nucleotides
Pf1N1B4_122 - Inner membrane component of tripartite multidrug resistance system, at 133,290 to 134,882
_122
Pf1N1B4_123 - 4-carboxymuconolactone decarboxylase domain/alkylhydroperoxidase AhpD family core domain protein, at 134,892 to 135,332
_123
Pf1N1B4_124 - Mechanosensitive ion channel, at 135,578 to 137,023
_124
Pf1N1B4_125 - Nicotinamidase family protein YcaC, at 137,213 to 137,839
_125
Pf1N1B4_126 - Pirin, at 137,967 to 138,833
_126
Position (kb) 135
136
137
138 Strain fitness (log2 ratio) -2
-1
0
1
2 at 134.675 kb on + strand, within Pf1N1B4_122 at 134.676 kb on - strand at 134.772 kb on + strand at 134.772 kb on + strand at 134.772 kb on + strand at 134.773 kb on - strand at 134.894 kb on - strand at 135.011 kb on - strand, within Pf1N1B4_123 at 135.011 kb on - strand, within Pf1N1B4_123 at 135.311 kb on - strand at 135.389 kb on - strand at 135.389 kb on - strand at 135.394 kb on + strand at 135.403 kb on - strand at 135.403 kb on - strand at 135.425 kb on + strand at 135.526 kb on + strand at 135.735 kb on + strand, within Pf1N1B4_124 at 136.647 kb on + strand, within Pf1N1B4_124 at 136.647 kb on + strand, within Pf1N1B4_124 at 136.648 kb on - strand, within Pf1N1B4_124 at 136.648 kb on - strand, within Pf1N1B4_124 at 137.040 kb on + strand at 137.041 kb on - strand at 137.043 kb on + strand at 137.044 kb on - strand at 137.044 kb on - strand at 137.060 kb on + strand at 137.061 kb on - strand at 137.061 kb on - strand at 137.061 kb on - strand at 137.128 kb on - strand at 137.189 kb on + strand at 137.189 kb on + strand at 137.197 kb on + strand at 137.197 kb on + strand at 137.197 kb on + strand at 137.197 kb on + strand at 137.197 kb on + strand at 137.198 kb on - strand at 137.198 kb on - strand at 137.198 kb on - strand at 137.198 kb on - strand at 137.198 kb on - strand at 137.214 kb on + strand at 137.215 kb on - strand at 137.238 kb on + strand at 137.239 kb on - strand at 137.239 kb on - strand at 137.695 kb on - strand, within Pf1N1B4_125 at 137.834 kb on - strand at 137.834 kb on - strand at 137.924 kb on + strand at 137.955 kb on - strand at 137.955 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 134,675 + Pf1N1B4_122 0.87 -1.2 134,676 - +1.0 134,772 + +0.9 134,772 + +2.2 134,772 + -1.2 134,773 - +0.4 134,894 - +0.4 135,011 - Pf1N1B4_123 0.27 -0.6 135,011 - Pf1N1B4_123 0.27 +0.4 135,311 - +1.0 135,389 - +1.0 135,389 - -1.9 135,394 + -0.0 135,403 - -0.2 135,403 - -0.3 135,425 + -0.3 135,526 + -0.5 135,735 + Pf1N1B4_124 0.11 -0.6 136,647 + Pf1N1B4_124 0.74 -0.5 136,647 + Pf1N1B4_124 0.74 -1.2 136,648 - Pf1N1B4_124 0.74 -1.9 136,648 - Pf1N1B4_124 0.74 -0.6 137,040 + -1.2 137,041 - -0.3 137,043 + -2.6 137,044 - +0.1 137,044 - +0.5 137,060 + +1.0 137,061 - -0.0 137,061 - +0.4 137,061 - -1.9 137,128 - -0.6 137,189 + +1.1 137,189 + +0.4 137,197 + +0.4 137,197 + +0.4 137,197 + +0.4 137,197 + -1.6 137,197 + -1.6 137,198 - +0.4 137,198 - -1.6 137,198 - +1.0 137,198 - -0.5 137,198 - +0.7 137,214 + -1.9 137,215 - -0.6 137,238 + -0.0 137,239 - +0.4 137,239 - +0.7 137,695 - Pf1N1B4_125 0.77 -0.1 137,834 - -0.2 137,834 - +0.4 137,924 + -1.9 137,955 - +0.2 137,955 - +0.9
Or see this region's nucleotide sequence