Experiment: Gentamicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1203 and Pf1N1B4_1204 are separated by 41 nucleotides Pf1N1B4_1204 and Pf1N1B4_1205 are separated by 251 nucleotides Pf1N1B4_1205 and Pf1N1B4_1206 overlap by 36 nucleotides Pf1N1B4_1206 and Pf1N1B4_1207 are separated by 13 nucleotides
Pf1N1B4_1203 - Putative insecticidal toxin complex, at 1,365,611 to 1,368,373
_1203
Pf1N1B4_1204 - Glyoxalase family protein, at 1,368,415 to 1,368,783
_1204
Pf1N1B4_1205 - Sulfate permease, at 1,369,035 to 1,370,420
_1205
Pf1N1B4_1206 - hypothetical protein, at 1,370,385 to 1,370,672
_1206
Pf1N1B4_1207 - Transcriptional regulator, LysR family, at 1,370,686 to 1,371,597
_1207
Position (kb)
1369
1370
1371 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1368.041 kb on - strand, within Pf1N1B4_1203 at 1368.041 kb on - strand, within Pf1N1B4_1203 at 1368.144 kb on + strand at 1368.157 kb on - strand at 1368.165 kb on + strand at 1368.165 kb on + strand at 1368.165 kb on + strand at 1368.166 kb on - strand at 1368.166 kb on - strand at 1368.250 kb on + strand at 1368.250 kb on + strand at 1368.251 kb on - strand at 1368.254 kb on + strand at 1368.254 kb on + strand at 1368.255 kb on - strand at 1368.255 kb on - strand at 1368.255 kb on - strand at 1368.265 kb on + strand at 1368.265 kb on + strand at 1368.265 kb on + strand at 1368.265 kb on + strand at 1368.361 kb on + strand at 1368.368 kb on + strand at 1368.368 kb on + strand at 1368.368 kb on + strand at 1368.387 kb on + strand at 1368.423 kb on + strand at 1368.423 kb on + strand at 1368.424 kb on - strand at 1368.424 kb on - strand at 1368.424 kb on - strand at 1368.524 kb on + strand, within Pf1N1B4_1204 at 1368.525 kb on - strand, within Pf1N1B4_1204 at 1368.656 kb on + strand, within Pf1N1B4_1204 at 1368.657 kb on - strand, within Pf1N1B4_1204 at 1368.657 kb on - strand, within Pf1N1B4_1204 at 1368.657 kb on - strand, within Pf1N1B4_1204 at 1368.657 kb on - strand, within Pf1N1B4_1204 at 1368.657 kb on - strand, within Pf1N1B4_1204 at 1368.657 kb on - strand, within Pf1N1B4_1204 at 1368.719 kb on + strand, within Pf1N1B4_1204 at 1368.719 kb on + strand, within Pf1N1B4_1204 at 1368.719 kb on + strand, within Pf1N1B4_1204 at 1368.720 kb on - strand, within Pf1N1B4_1204 at 1368.765 kb on + strand at 1368.817 kb on + strand at 1368.817 kb on + strand at 1368.817 kb on + strand at 1368.817 kb on + strand at 1368.817 kb on + strand at 1368.817 kb on + strand at 1368.818 kb on - strand at 1368.902 kb on + strand at 1368.902 kb on + strand at 1368.919 kb on + strand at 1368.939 kb on + strand at 1368.939 kb on + strand at 1368.940 kb on - strand at 1369.014 kb on - strand at 1369.102 kb on - strand at 1369.123 kb on + strand at 1369.124 kb on - strand at 1369.163 kb on - strand at 1369.163 kb on - strand at 1369.247 kb on + strand, within Pf1N1B4_1205 at 1369.247 kb on + strand, within Pf1N1B4_1205 at 1369.318 kb on + strand, within Pf1N1B4_1205 at 1369.318 kb on + strand, within Pf1N1B4_1205 at 1369.318 kb on + strand, within Pf1N1B4_1205 at 1369.318 kb on + strand, within Pf1N1B4_1205 at 1369.319 kb on - strand, within Pf1N1B4_1205 at 1369.406 kb on + strand, within Pf1N1B4_1205 at 1369.580 kb on + strand, within Pf1N1B4_1205 at 1369.581 kb on - strand, within Pf1N1B4_1205 at 1369.581 kb on - strand, within Pf1N1B4_1205 at 1369.814 kb on - strand, within Pf1N1B4_1205 at 1369.814 kb on - strand, within Pf1N1B4_1205 at 1370.047 kb on + strand, within Pf1N1B4_1205 at 1370.047 kb on + strand, within Pf1N1B4_1205 at 1370.047 kb on + strand, within Pf1N1B4_1205 at 1370.047 kb on + strand, within Pf1N1B4_1205 at 1370.047 kb on + strand, within Pf1N1B4_1205 at 1370.047 kb on + strand, within Pf1N1B4_1205 at 1370.048 kb on - strand, within Pf1N1B4_1205 at 1370.048 kb on - strand at 1370.239 kb on + strand, within Pf1N1B4_1205 at 1370.239 kb on + strand, within Pf1N1B4_1205 at 1370.239 kb on + strand, within Pf1N1B4_1205 at 1370.239 kb on + strand, within Pf1N1B4_1205 at 1370.240 kb on - strand, within Pf1N1B4_1205 at 1370.240 kb on - strand, within Pf1N1B4_1205 at 1370.240 kb on - strand, within Pf1N1B4_1205 at 1370.240 kb on - strand, within Pf1N1B4_1205 at 1370.362 kb on + strand at 1370.362 kb on + strand at 1370.363 kb on - strand at 1370.486 kb on - strand, within Pf1N1B4_1206 at 1370.490 kb on - strand, within Pf1N1B4_1206 at 1370.712 kb on - strand at 1370.784 kb on - strand, within Pf1N1B4_1207 at 1370.784 kb on - strand, within Pf1N1B4_1207 at 1370.784 kb on - strand, within Pf1N1B4_1207 at 1370.981 kb on + strand, within Pf1N1B4_1207 at 1370.981 kb on + strand, within Pf1N1B4_1207 at 1370.982 kb on - strand at 1371.083 kb on + strand, within Pf1N1B4_1207 at 1371.084 kb on - strand, within Pf1N1B4_1207 at 1371.266 kb on + strand, within Pf1N1B4_1207 at 1371.266 kb on + strand, within Pf1N1B4_1207 at 1371.267 kb on - strand, within Pf1N1B4_1207 at 1371.404 kb on + strand, within Pf1N1B4_1207 at 1371.404 kb on + strand, within Pf1N1B4_1207 at 1371.404 kb on + strand, within Pf1N1B4_1207 at 1371.405 kb on - strand, within Pf1N1B4_1207 at 1371.405 kb on - strand, within Pf1N1B4_1207 at 1371.405 kb on - strand, within Pf1N1B4_1207
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.001 mg/ml remove 1,368,041 - Pf1N1B4_1203 0.88 -0.0 1,368,041 - Pf1N1B4_1203 0.88 +0.6 1,368,144 + +0.2 1,368,157 - -1.6 1,368,165 + -0.6 1,368,165 + +0.8 1,368,165 + -1.4 1,368,166 - -0.6 1,368,166 - +1.6 1,368,250 + +0.6 1,368,250 + -0.3 1,368,251 - -0.3 1,368,254 + -0.0 1,368,254 + -1.2 1,368,255 - -1.0 1,368,255 - +0.6 1,368,255 - -0.0 1,368,265 + -0.0 1,368,265 + -1.8 1,368,265 + -0.3 1,368,265 + -1.6 1,368,361 + +1.0 1,368,368 + -0.0 1,368,368 + -0.4 1,368,368 + -0.4 1,368,387 + -0.4 1,368,423 + -0.0 1,368,423 + +0.7 1,368,424 - -0.0 1,368,424 - -0.2 1,368,424 - -0.0 1,368,524 + Pf1N1B4_1204 0.30 +0.2 1,368,525 - Pf1N1B4_1204 0.30 +0.3 1,368,656 + Pf1N1B4_1204 0.65 -0.4 1,368,657 - Pf1N1B4_1204 0.66 -0.2 1,368,657 - Pf1N1B4_1204 0.66 +1.4 1,368,657 - Pf1N1B4_1204 0.66 +0.1 1,368,657 - Pf1N1B4_1204 0.66 -1.4 1,368,657 - Pf1N1B4_1204 0.66 +2.3 1,368,657 - Pf1N1B4_1204 0.66 -0.9 1,368,719 + Pf1N1B4_1204 0.82 -0.9 1,368,719 + Pf1N1B4_1204 0.82 -0.4 1,368,719 + Pf1N1B4_1204 0.82 +0.2 1,368,720 - Pf1N1B4_1204 0.83 +0.8 1,368,765 + -0.5 1,368,817 + +0.4 1,368,817 + +1.0 1,368,817 + -1.0 1,368,817 + -0.6 1,368,817 + +0.8 1,368,817 + -1.8 1,368,818 - -1.0 1,368,902 + +0.6 1,368,902 + -0.9 1,368,919 + +1.4 1,368,939 + +0.5 1,368,939 + -1.0 1,368,940 - +0.4 1,369,014 - -0.0 1,369,102 - -1.3 1,369,123 + -0.6 1,369,124 - -0.0 1,369,163 - -0.5 1,369,163 - +1.6 1,369,247 + Pf1N1B4_1205 0.15 +1.6 1,369,247 + Pf1N1B4_1205 0.15 -1.6 1,369,318 + Pf1N1B4_1205 0.20 +0.2 1,369,318 + Pf1N1B4_1205 0.20 +0.2 1,369,318 + Pf1N1B4_1205 0.20 -0.6 1,369,318 + Pf1N1B4_1205 0.20 -1.9 1,369,319 - Pf1N1B4_1205 0.20 +0.2 1,369,406 + Pf1N1B4_1205 0.27 -2.2 1,369,580 + Pf1N1B4_1205 0.39 -1.0 1,369,581 - Pf1N1B4_1205 0.39 -1.0 1,369,581 - Pf1N1B4_1205 0.39 -0.1 1,369,814 - Pf1N1B4_1205 0.56 -1.0 1,369,814 - Pf1N1B4_1205 0.56 -1.2 1,370,047 + Pf1N1B4_1205 0.73 -1.6 1,370,047 + Pf1N1B4_1205 0.73 +1.6 1,370,047 + Pf1N1B4_1205 0.73 -0.3 1,370,047 + Pf1N1B4_1205 0.73 -2.0 1,370,047 + Pf1N1B4_1205 0.73 -1.4 1,370,047 + Pf1N1B4_1205 0.73 -1.3 1,370,048 - Pf1N1B4_1205 0.73 -0.9 1,370,048 - -1.0 1,370,239 + Pf1N1B4_1205 0.87 -0.0 1,370,239 + Pf1N1B4_1205 0.87 +1.4 1,370,239 + Pf1N1B4_1205 0.87 -2.4 1,370,239 + Pf1N1B4_1205 0.87 +0.4 1,370,240 - Pf1N1B4_1205 0.87 -0.6 1,370,240 - Pf1N1B4_1205 0.87 -1.4 1,370,240 - Pf1N1B4_1205 0.87 -0.0 1,370,240 - Pf1N1B4_1205 0.87 -0.9 1,370,362 + -1.8 1,370,362 + -0.8 1,370,363 - -0.4 1,370,486 - Pf1N1B4_1206 0.35 +1.0 1,370,490 - Pf1N1B4_1206 0.36 +1.5 1,370,712 - -1.4 1,370,784 - Pf1N1B4_1207 0.11 -0.2 1,370,784 - Pf1N1B4_1207 0.11 +0.2 1,370,784 - Pf1N1B4_1207 0.11 +0.5 1,370,981 + Pf1N1B4_1207 0.32 -0.4 1,370,981 + Pf1N1B4_1207 0.32 -0.0 1,370,982 - +0.4 1,371,083 + Pf1N1B4_1207 0.44 +1.3 1,371,084 - Pf1N1B4_1207 0.44 -0.3 1,371,266 + Pf1N1B4_1207 0.64 +1.3 1,371,266 + Pf1N1B4_1207 0.64 -1.2 1,371,267 - Pf1N1B4_1207 0.64 +0.7 1,371,404 + Pf1N1B4_1207 0.79 -0.8 1,371,404 + Pf1N1B4_1207 0.79 +0.7 1,371,404 + Pf1N1B4_1207 0.79 -2.5 1,371,405 - Pf1N1B4_1207 0.79 +1.6 1,371,405 - Pf1N1B4_1207 0.79 -0.3 1,371,405 - Pf1N1B4_1207 0.79 +1.5
Or see this region's nucleotide sequence