Experiment: formate McAA folic dropout
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01090 and MMJJ_RS01095 are separated by 130 nucleotides MMJJ_RS01095 and MMJJ_RS01100 are separated by 189 nucleotides MMJJ_RS01100 and MMJJ_RS01105 overlap by 8 nucleotides
MMJJ_RS01090: MMJJ_RS01090 - hypothetical protein, at 210,788 to 211,474
_RS01090
MMJJ_RS01095: MMJJ_RS01095 - site-specific integrase, at 211,605 to 212,603
_RS01095
MMJJ_RS01100: MMJJ_RS01100 - helix-turn-helix domain-containing protein, at 212,793 to 213,035
_RS01100
MMJJ_RS01105: MMJJ_RS01105 - DUF5906 domain-containing protein, at 213,028 to 215,544
_RS01105
Position (kb)
211
212
213 Strain fitness (log2 ratio)
-2
-1
0
1 at 210.761 kb on - strand at 210.762 kb on + strand at 210.762 kb on + strand at 210.762 kb on + strand at 210.805 kb on - strand at 210.806 kb on + strand at 210.823 kb on - strand at 210.824 kb on + strand at 210.831 kb on - strand at 210.832 kb on + strand at 210.848 kb on - strand at 210.849 kb on + strand at 210.853 kb on - strand at 210.858 kb on + strand, within MMJJ_RS01090 at 210.932 kb on + strand, within MMJJ_RS01090 at 211.193 kb on + strand, within MMJJ_RS01090 at 211.215 kb on - strand, within MMJJ_RS01090 at 211.238 kb on + strand, within MMJJ_RS01090 at 211.240 kb on + strand, within MMJJ_RS01090 at 211.240 kb on + strand, within MMJJ_RS01090 at 211.240 kb on + strand, within MMJJ_RS01090 at 211.262 kb on + strand, within MMJJ_RS01090 at 211.264 kb on - strand, within MMJJ_RS01090 at 211.282 kb on - strand, within MMJJ_RS01090 at 211.297 kb on - strand, within MMJJ_RS01090 at 211.297 kb on - strand, within MMJJ_RS01090 at 211.298 kb on + strand, within MMJJ_RS01090 at 211.298 kb on + strand, within MMJJ_RS01090 at 211.298 kb on + strand, within MMJJ_RS01090 at 211.403 kb on - strand, within MMJJ_RS01090 at 211.442 kb on + strand at 211.475 kb on + strand at 211.496 kb on - strand at 211.500 kb on + strand at 211.500 kb on + strand at 211.502 kb on + strand at 211.502 kb on + strand at 211.504 kb on + strand at 211.507 kb on - strand at 211.507 kb on - strand at 211.508 kb on + strand at 211.508 kb on + strand at 211.510 kb on + strand at 211.541 kb on - strand at 211.542 kb on + strand at 211.546 kb on + strand at 211.639 kb on - strand at 211.640 kb on + strand at 211.640 kb on + strand at 211.731 kb on + strand, within MMJJ_RS01095 at 211.742 kb on - strand, within MMJJ_RS01095 at 211.742 kb on - strand, within MMJJ_RS01095 at 211.743 kb on + strand, within MMJJ_RS01095 at 211.743 kb on + strand, within MMJJ_RS01095 at 211.853 kb on - strand, within MMJJ_RS01095 at 211.854 kb on + strand, within MMJJ_RS01095 at 211.893 kb on + strand, within MMJJ_RS01095 at 211.943 kb on - strand, within MMJJ_RS01095 at 211.944 kb on + strand, within MMJJ_RS01095 at 211.944 kb on + strand, within MMJJ_RS01095 at 212.095 kb on - strand, within MMJJ_RS01095 at 212.145 kb on + strand, within MMJJ_RS01095 at 212.151 kb on + strand, within MMJJ_RS01095 at 212.158 kb on - strand, within MMJJ_RS01095 at 212.159 kb on + strand, within MMJJ_RS01095 at 212.159 kb on + strand, within MMJJ_RS01095 at 212.237 kb on - strand, within MMJJ_RS01095 at 212.367 kb on - strand, within MMJJ_RS01095 at 212.368 kb on + strand, within MMJJ_RS01095 at 212.440 kb on - strand, within MMJJ_RS01095 at 212.440 kb on - strand, within MMJJ_RS01095 at 212.467 kb on - strand, within MMJJ_RS01095 at 212.467 kb on - strand, within MMJJ_RS01095 at 212.468 kb on + strand, within MMJJ_RS01095 at 212.468 kb on + strand, within MMJJ_RS01095 at 212.485 kb on - strand, within MMJJ_RS01095 at 212.485 kb on - strand, within MMJJ_RS01095 at 212.532 kb on + strand at 212.539 kb on + strand at 212.558 kb on - strand at 212.629 kb on - strand at 212.629 kb on - strand at 212.668 kb on - strand at 212.669 kb on + strand at 212.672 kb on - strand at 212.715 kb on + strand at 212.716 kb on - strand at 212.717 kb on + strand at 212.717 kb on + strand at 212.717 kb on + strand at 212.718 kb on - strand at 212.718 kb on - strand at 212.719 kb on + strand at 212.719 kb on + strand at 212.720 kb on - strand at 212.720 kb on - strand at 212.753 kb on - strand at 212.777 kb on + strand at 212.814 kb on - strand at 212.814 kb on - strand at 212.814 kb on - strand at 212.814 kb on - strand at 212.814 kb on - strand at 212.814 kb on - strand at 212.816 kb on - strand at 212.817 kb on + strand at 212.822 kb on + strand, within MMJJ_RS01100 at 212.853 kb on - strand, within MMJJ_RS01100 at 213.025 kb on - strand at 213.025 kb on - strand at 213.026 kb on + strand at 213.026 kb on + strand at 213.026 kb on + strand at 213.034 kb on + strand at 213.038 kb on - strand at 213.038 kb on - strand at 213.038 kb on - strand at 213.039 kb on + strand at 213.039 kb on + strand at 213.081 kb on + strand at 213.090 kb on - strand at 213.098 kb on - strand at 213.170 kb on + strand at 213.264 kb on - strand at 213.283 kb on - strand, within MMJJ_RS01105 at 213.283 kb on - strand, within MMJJ_RS01105 at 213.288 kb on - strand, within MMJJ_RS01105 at 213.289 kb on + strand, within MMJJ_RS01105 at 213.339 kb on - strand, within MMJJ_RS01105 at 213.340 kb on + strand, within MMJJ_RS01105 at 213.340 kb on + strand, within MMJJ_RS01105 at 213.344 kb on + strand, within MMJJ_RS01105 at 213.352 kb on - strand, within MMJJ_RS01105 at 213.352 kb on - strand, within MMJJ_RS01105 at 213.358 kb on - strand, within MMJJ_RS01105 at 213.424 kb on + strand, within MMJJ_RS01105 at 213.479 kb on - strand, within MMJJ_RS01105 at 213.541 kb on + strand, within MMJJ_RS01105
Per-strain Table
Position Strand Gene LocusTag Fraction formate McAA folic dropout remove 210,761 - +0.9 210,762 + -1.6 210,762 + +0.6 210,762 + +0.5 210,805 - +0.4 210,806 + +0.2 210,823 - -0.4 210,824 + +0.6 210,831 - +1.4 210,832 + +0.2 210,848 - -0.5 210,849 + -0.8 210,853 - +0.2 210,858 + MMJJ_RS01090 0.10 -0.1 210,932 + MMJJ_RS01090 0.21 -0.3 211,193 + MMJJ_RS01090 0.59 -1.2 211,215 - MMJJ_RS01090 0.62 -0.8 211,238 + MMJJ_RS01090 0.66 -0.2 211,240 + MMJJ_RS01090 0.66 -0.6 211,240 + MMJJ_RS01090 0.66 -0.4 211,240 + MMJJ_RS01090 0.66 +0.2 211,262 + MMJJ_RS01090 0.69 -0.3 211,264 - MMJJ_RS01090 0.69 -0.6 211,282 - MMJJ_RS01090 0.72 +0.5 211,297 - MMJJ_RS01090 0.74 +0.1 211,297 - MMJJ_RS01090 0.74 -0.5 211,298 + MMJJ_RS01090 0.74 +0.4 211,298 + MMJJ_RS01090 0.74 -1.5 211,298 + MMJJ_RS01090 0.74 -0.1 211,403 - MMJJ_RS01090 0.90 +0.6 211,442 + -0.5 211,475 + -2.0 211,496 - -0.8 211,500 + -1.1 211,500 + -2.1 211,502 + -1.5 211,502 + -0.2 211,504 + -0.1 211,507 - +1.2 211,507 - -0.7 211,508 + -0.7 211,508 + +0.1 211,510 + -0.3 211,541 - +0.4 211,542 + +0.1 211,546 + -0.3 211,639 - -0.1 211,640 + +0.5 211,640 + +0.7 211,731 + MMJJ_RS01095 0.13 +0.1 211,742 - MMJJ_RS01095 0.14 +0.3 211,742 - MMJJ_RS01095 0.14 -0.4 211,743 + MMJJ_RS01095 0.14 +0.3 211,743 + MMJJ_RS01095 0.14 -0.4 211,853 - MMJJ_RS01095 0.25 +0.0 211,854 + MMJJ_RS01095 0.25 +0.3 211,893 + MMJJ_RS01095 0.29 +0.5 211,943 - MMJJ_RS01095 0.34 -0.7 211,944 + MMJJ_RS01095 0.34 +0.0 211,944 + MMJJ_RS01095 0.34 +0.1 212,095 - MMJJ_RS01095 0.49 +0.1 212,145 + MMJJ_RS01095 0.54 -0.2 212,151 + MMJJ_RS01095 0.55 -0.2 212,158 - MMJJ_RS01095 0.55 +0.8 212,159 + MMJJ_RS01095 0.55 -0.2 212,159 + MMJJ_RS01095 0.55 +1.4 212,237 - MMJJ_RS01095 0.63 +0.1 212,367 - MMJJ_RS01095 0.76 +0.8 212,368 + MMJJ_RS01095 0.76 +0.5 212,440 - MMJJ_RS01095 0.84 +0.1 212,440 - MMJJ_RS01095 0.84 -0.4 212,467 - MMJJ_RS01095 0.86 +0.4 212,467 - MMJJ_RS01095 0.86 +0.7 212,468 + MMJJ_RS01095 0.86 +1.1 212,468 + MMJJ_RS01095 0.86 +0.3 212,485 - MMJJ_RS01095 0.88 +0.7 212,485 - MMJJ_RS01095 0.88 +0.3 212,532 + -1.1 212,539 + -1.1 212,558 - +0.3 212,629 - -0.1 212,629 - -1.3 212,668 - -0.0 212,669 + +0.8 212,672 - -0.2 212,715 + -0.8 212,716 - -1.7 212,717 + -1.5 212,717 + -1.8 212,717 + -0.3 212,718 - +0.2 212,718 - +0.3 212,719 + -0.1 212,719 + +0.9 212,720 - +0.2 212,720 - -0.8 212,753 - +1.1 212,777 + -0.2 212,814 - +0.1 212,814 - +0.3 212,814 - -0.0 212,814 - -0.3 212,814 - +0.6 212,814 - +0.1 212,816 - -0.6 212,817 + -2.4 212,822 + MMJJ_RS01100 0.12 -0.1 212,853 - MMJJ_RS01100 0.25 -0.1 213,025 - +1.0 213,025 - -0.6 213,026 + -0.8 213,026 + +0.2 213,026 + +0.9 213,034 + -0.1 213,038 - +1.0 213,038 - -0.9 213,038 - +0.6 213,039 + +0.4 213,039 + -0.4 213,081 + -0.0 213,090 - -1.9 213,098 - +0.3 213,170 + -0.6 213,264 - +0.2 213,283 - MMJJ_RS01105 0.10 -0.1 213,283 - MMJJ_RS01105 0.10 -0.3 213,288 - MMJJ_RS01105 0.10 -0.1 213,289 + MMJJ_RS01105 0.10 -0.4 213,339 - MMJJ_RS01105 0.12 -0.5 213,340 + MMJJ_RS01105 0.12 +0.2 213,340 + MMJJ_RS01105 0.12 +0.6 213,344 + MMJJ_RS01105 0.13 -0.4 213,352 - MMJJ_RS01105 0.13 +0.8 213,352 - MMJJ_RS01105 0.13 -0.3 213,358 - MMJJ_RS01105 0.13 -0.5 213,424 + MMJJ_RS01105 0.16 -0.4 213,479 - MMJJ_RS01105 0.18 +0.6 213,541 + MMJJ_RS01105 0.20 -0.0
Or see this region's nucleotide sequence