Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS01095

Experiment: formate McAA folic dropout

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS01090 and MMJJ_RS01095 are separated by 130 nucleotidesMMJJ_RS01095 and MMJJ_RS01100 are separated by 189 nucleotidesMMJJ_RS01100 and MMJJ_RS01105 overlap by 8 nucleotides MMJJ_RS01090: MMJJ_RS01090 - hypothetical protein, at 210,788 to 211,474 _RS01090 MMJJ_RS01095: MMJJ_RS01095 - site-specific integrase, at 211,605 to 212,603 _RS01095 MMJJ_RS01100: MMJJ_RS01100 - helix-turn-helix domain-containing protein, at 212,793 to 213,035 _RS01100 MMJJ_RS01105: MMJJ_RS01105 - DUF5906 domain-containing protein, at 213,028 to 215,544 _RS01105 Position (kb) 211 212 213Strain fitness (log2 ratio) -2 -1 0 1at 210.761 kb on - strandat 210.762 kb on + strandat 210.762 kb on + strandat 210.762 kb on + strandat 210.805 kb on - strandat 210.806 kb on + strandat 210.823 kb on - strandat 210.824 kb on + strandat 210.831 kb on - strandat 210.832 kb on + strandat 210.848 kb on - strandat 210.849 kb on + strandat 210.853 kb on - strandat 210.858 kb on + strand, within MMJJ_RS01090at 210.932 kb on + strand, within MMJJ_RS01090at 211.193 kb on + strand, within MMJJ_RS01090at 211.215 kb on - strand, within MMJJ_RS01090at 211.238 kb on + strand, within MMJJ_RS01090at 211.240 kb on + strand, within MMJJ_RS01090at 211.240 kb on + strand, within MMJJ_RS01090at 211.240 kb on + strand, within MMJJ_RS01090at 211.262 kb on + strand, within MMJJ_RS01090at 211.264 kb on - strand, within MMJJ_RS01090at 211.282 kb on - strand, within MMJJ_RS01090at 211.297 kb on - strand, within MMJJ_RS01090at 211.297 kb on - strand, within MMJJ_RS01090at 211.298 kb on + strand, within MMJJ_RS01090at 211.298 kb on + strand, within MMJJ_RS01090at 211.298 kb on + strand, within MMJJ_RS01090at 211.403 kb on - strand, within MMJJ_RS01090at 211.442 kb on + strandat 211.475 kb on + strandat 211.496 kb on - strandat 211.500 kb on + strandat 211.500 kb on + strandat 211.502 kb on + strandat 211.502 kb on + strandat 211.504 kb on + strandat 211.507 kb on - strandat 211.507 kb on - strandat 211.508 kb on + strandat 211.508 kb on + strandat 211.510 kb on + strandat 211.541 kb on - strandat 211.542 kb on + strandat 211.546 kb on + strandat 211.639 kb on - strandat 211.640 kb on + strandat 211.640 kb on + strandat 211.731 kb on + strand, within MMJJ_RS01095at 211.742 kb on - strand, within MMJJ_RS01095at 211.742 kb on - strand, within MMJJ_RS01095at 211.743 kb on + strand, within MMJJ_RS01095at 211.743 kb on + strand, within MMJJ_RS01095at 211.853 kb on - strand, within MMJJ_RS01095at 211.854 kb on + strand, within MMJJ_RS01095at 211.893 kb on + strand, within MMJJ_RS01095at 211.943 kb on - strand, within MMJJ_RS01095at 211.944 kb on + strand, within MMJJ_RS01095at 211.944 kb on + strand, within MMJJ_RS01095at 212.095 kb on - strand, within MMJJ_RS01095at 212.145 kb on + strand, within MMJJ_RS01095at 212.151 kb on + strand, within MMJJ_RS01095at 212.158 kb on - strand, within MMJJ_RS01095at 212.159 kb on + strand, within MMJJ_RS01095at 212.159 kb on + strand, within MMJJ_RS01095at 212.237 kb on - strand, within MMJJ_RS01095at 212.367 kb on - strand, within MMJJ_RS01095at 212.368 kb on + strand, within MMJJ_RS01095at 212.440 kb on - strand, within MMJJ_RS01095at 212.440 kb on - strand, within MMJJ_RS01095at 212.467 kb on - strand, within MMJJ_RS01095at 212.467 kb on - strand, within MMJJ_RS01095at 212.468 kb on + strand, within MMJJ_RS01095at 212.468 kb on + strand, within MMJJ_RS01095at 212.485 kb on - strand, within MMJJ_RS01095at 212.485 kb on - strand, within MMJJ_RS01095at 212.532 kb on + strandat 212.539 kb on + strandat 212.558 kb on - strandat 212.629 kb on - strandat 212.629 kb on - strandat 212.668 kb on - strandat 212.669 kb on + strandat 212.672 kb on - strandat 212.715 kb on + strandat 212.716 kb on - strandat 212.717 kb on + strandat 212.717 kb on + strandat 212.717 kb on + strandat 212.718 kb on - strandat 212.718 kb on - strandat 212.719 kb on + strandat 212.719 kb on + strandat 212.720 kb on - strandat 212.720 kb on - strandat 212.753 kb on - strandat 212.777 kb on + strandat 212.814 kb on - strandat 212.814 kb on - strandat 212.814 kb on - strandat 212.814 kb on - strandat 212.814 kb on - strandat 212.814 kb on - strandat 212.816 kb on - strandat 212.817 kb on + strandat 212.822 kb on + strand, within MMJJ_RS01100at 212.853 kb on - strand, within MMJJ_RS01100at 213.025 kb on - strandat 213.025 kb on - strandat 213.026 kb on + strandat 213.026 kb on + strandat 213.026 kb on + strandat 213.034 kb on + strandat 213.038 kb on - strandat 213.038 kb on - strandat 213.038 kb on - strandat 213.039 kb on + strandat 213.039 kb on + strandat 213.081 kb on + strandat 213.090 kb on - strandat 213.098 kb on - strandat 213.170 kb on + strandat 213.264 kb on - strandat 213.283 kb on - strand, within MMJJ_RS01105at 213.283 kb on - strand, within MMJJ_RS01105at 213.288 kb on - strand, within MMJJ_RS01105at 213.289 kb on + strand, within MMJJ_RS01105at 213.339 kb on - strand, within MMJJ_RS01105at 213.340 kb on + strand, within MMJJ_RS01105at 213.340 kb on + strand, within MMJJ_RS01105at 213.344 kb on + strand, within MMJJ_RS01105at 213.352 kb on - strand, within MMJJ_RS01105at 213.352 kb on - strand, within MMJJ_RS01105at 213.358 kb on - strand, within MMJJ_RS01105at 213.424 kb on + strand, within MMJJ_RS01105at 213.479 kb on - strand, within MMJJ_RS01105at 213.541 kb on + strand, within MMJJ_RS01105

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Per-strain Table

Position Strand Gene LocusTag Fraction formate McAA folic dropout
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210,761 - +0.9
210,762 + -1.6
210,762 + +0.6
210,762 + +0.5
210,805 - +0.4
210,806 + +0.2
210,823 - -0.4
210,824 + +0.6
210,831 - +1.4
210,832 + +0.2
210,848 - -0.5
210,849 + -0.8
210,853 - +0.2
210,858 + MMJJ_RS01090 0.10 -0.1
210,932 + MMJJ_RS01090 0.21 -0.3
211,193 + MMJJ_RS01090 0.59 -1.2
211,215 - MMJJ_RS01090 0.62 -0.8
211,238 + MMJJ_RS01090 0.66 -0.2
211,240 + MMJJ_RS01090 0.66 -0.6
211,240 + MMJJ_RS01090 0.66 -0.4
211,240 + MMJJ_RS01090 0.66 +0.2
211,262 + MMJJ_RS01090 0.69 -0.3
211,264 - MMJJ_RS01090 0.69 -0.6
211,282 - MMJJ_RS01090 0.72 +0.5
211,297 - MMJJ_RS01090 0.74 +0.1
211,297 - MMJJ_RS01090 0.74 -0.5
211,298 + MMJJ_RS01090 0.74 +0.4
211,298 + MMJJ_RS01090 0.74 -1.5
211,298 + MMJJ_RS01090 0.74 -0.1
211,403 - MMJJ_RS01090 0.90 +0.6
211,442 + -0.5
211,475 + -2.0
211,496 - -0.8
211,500 + -1.1
211,500 + -2.1
211,502 + -1.5
211,502 + -0.2
211,504 + -0.1
211,507 - +1.2
211,507 - -0.7
211,508 + -0.7
211,508 + +0.1
211,510 + -0.3
211,541 - +0.4
211,542 + +0.1
211,546 + -0.3
211,639 - -0.1
211,640 + +0.5
211,640 + +0.7
211,731 + MMJJ_RS01095 0.13 +0.1
211,742 - MMJJ_RS01095 0.14 +0.3
211,742 - MMJJ_RS01095 0.14 -0.4
211,743 + MMJJ_RS01095 0.14 +0.3
211,743 + MMJJ_RS01095 0.14 -0.4
211,853 - MMJJ_RS01095 0.25 +0.0
211,854 + MMJJ_RS01095 0.25 +0.3
211,893 + MMJJ_RS01095 0.29 +0.5
211,943 - MMJJ_RS01095 0.34 -0.7
211,944 + MMJJ_RS01095 0.34 +0.0
211,944 + MMJJ_RS01095 0.34 +0.1
212,095 - MMJJ_RS01095 0.49 +0.1
212,145 + MMJJ_RS01095 0.54 -0.2
212,151 + MMJJ_RS01095 0.55 -0.2
212,158 - MMJJ_RS01095 0.55 +0.8
212,159 + MMJJ_RS01095 0.55 -0.2
212,159 + MMJJ_RS01095 0.55 +1.4
212,237 - MMJJ_RS01095 0.63 +0.1
212,367 - MMJJ_RS01095 0.76 +0.8
212,368 + MMJJ_RS01095 0.76 +0.5
212,440 - MMJJ_RS01095 0.84 +0.1
212,440 - MMJJ_RS01095 0.84 -0.4
212,467 - MMJJ_RS01095 0.86 +0.4
212,467 - MMJJ_RS01095 0.86 +0.7
212,468 + MMJJ_RS01095 0.86 +1.1
212,468 + MMJJ_RS01095 0.86 +0.3
212,485 - MMJJ_RS01095 0.88 +0.7
212,485 - MMJJ_RS01095 0.88 +0.3
212,532 + -1.1
212,539 + -1.1
212,558 - +0.3
212,629 - -0.1
212,629 - -1.3
212,668 - -0.0
212,669 + +0.8
212,672 - -0.2
212,715 + -0.8
212,716 - -1.7
212,717 + -1.5
212,717 + -1.8
212,717 + -0.3
212,718 - +0.2
212,718 - +0.3
212,719 + -0.1
212,719 + +0.9
212,720 - +0.2
212,720 - -0.8
212,753 - +1.1
212,777 + -0.2
212,814 - +0.1
212,814 - +0.3
212,814 - -0.0
212,814 - -0.3
212,814 - +0.6
212,814 - +0.1
212,816 - -0.6
212,817 + -2.4
212,822 + MMJJ_RS01100 0.12 -0.1
212,853 - MMJJ_RS01100 0.25 -0.1
213,025 - +1.0
213,025 - -0.6
213,026 + -0.8
213,026 + +0.2
213,026 + +0.9
213,034 + -0.1
213,038 - +1.0
213,038 - -0.9
213,038 - +0.6
213,039 + +0.4
213,039 + -0.4
213,081 + -0.0
213,090 - -1.9
213,098 - +0.3
213,170 + -0.6
213,264 - +0.2
213,283 - MMJJ_RS01105 0.10 -0.1
213,283 - MMJJ_RS01105 0.10 -0.3
213,288 - MMJJ_RS01105 0.10 -0.1
213,289 + MMJJ_RS01105 0.10 -0.4
213,339 - MMJJ_RS01105 0.12 -0.5
213,340 + MMJJ_RS01105 0.12 +0.2
213,340 + MMJJ_RS01105 0.12 +0.6
213,344 + MMJJ_RS01105 0.13 -0.4
213,352 - MMJJ_RS01105 0.13 +0.8
213,352 - MMJJ_RS01105 0.13 -0.3
213,358 - MMJJ_RS01105 0.13 -0.5
213,424 + MMJJ_RS01105 0.16 -0.4
213,479 - MMJJ_RS01105 0.18 +0.6
213,541 + MMJJ_RS01105 0.20 -0.0

Or see this region's nucleotide sequence