Experiment: Plant assay with Setaria viridis; 10 days of inoculation with plant; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS18835 and HSERO_RS18840 are separated by 158 nucleotides HSERO_RS18840 and HSERO_RS18845 are separated by 94 nucleotides HSERO_RS18845 and HSERO_RS18850 are separated by 18 nucleotides
HSERO_RS18835: HSERO_RS18835 - hypothetical protein, at 4,321,295 to 4,321,834
_RS18835
HSERO_RS18840: HSERO_RS18840 - enoyl-CoA hydratase, at 4,321,993 to 4,322,784
_RS18840
HSERO_RS18845: HSERO_RS18845 - hypothetical protein, at 4,322,879 to 4,323,451
_RS18845
HSERO_RS18850: HSERO_RS18850 - transcriptional regulator, at 4,323,470 to 4,324,180
_RS18850
Position (kb)
4321
4322
4323 Strain fitness (log2 ratio)
-2
-1
0
1 at 4321.022 kb on + strand at 4321.022 kb on + strand at 4321.030 kb on - strand at 4321.030 kb on - strand at 4321.052 kb on + strand at 4321.057 kb on + strand at 4321.057 kb on + strand at 4321.064 kb on - strand at 4321.065 kb on - strand at 4321.065 kb on - strand at 4321.074 kb on - strand at 4321.076 kb on - strand at 4321.079 kb on - strand at 4321.092 kb on - strand at 4321.099 kb on - strand at 4321.120 kb on - strand at 4321.435 kb on - strand, within HSERO_RS18835 at 4321.444 kb on - strand, within HSERO_RS18835 at 4321.445 kb on + strand, within HSERO_RS18835 at 4321.455 kb on - strand, within HSERO_RS18835 at 4321.458 kb on - strand, within HSERO_RS18835 at 4321.496 kb on + strand, within HSERO_RS18835 at 4321.496 kb on + strand, within HSERO_RS18835 at 4321.829 kb on + strand at 4321.884 kb on + strand at 4321.900 kb on + strand at 4321.944 kb on - strand at 4321.954 kb on - strand at 4322.189 kb on - strand, within HSERO_RS18840 at 4322.319 kb on - strand, within HSERO_RS18840 at 4322.319 kb on - strand, within HSERO_RS18840 at 4322.325 kb on - strand, within HSERO_RS18840 at 4322.327 kb on + strand, within HSERO_RS18840 at 4322.418 kb on + strand, within HSERO_RS18840 at 4322.421 kb on - strand, within HSERO_RS18840 at 4322.426 kb on - strand, within HSERO_RS18840 at 4322.451 kb on + strand, within HSERO_RS18840 at 4322.522 kb on + strand, within HSERO_RS18840 at 4322.531 kb on + strand, within HSERO_RS18840 at 4322.639 kb on - strand, within HSERO_RS18840 at 4322.707 kb on + strand at 4322.744 kb on + strand at 4322.788 kb on - strand at 4322.788 kb on - strand at 4322.918 kb on - strand at 4322.920 kb on + strand at 4322.920 kb on + strand at 4322.934 kb on + strand at 4323.096 kb on + strand, within HSERO_RS18845 at 4323.101 kb on + strand, within HSERO_RS18845 at 4323.140 kb on - strand, within HSERO_RS18845 at 4323.177 kb on + strand at 4323.453 kb on + strand at 4323.516 kb on + strand at 4323.571 kb on + strand, within HSERO_RS18850 at 4323.731 kb on + strand, within HSERO_RS18850
Per-strain Table
Position Strand Gene LocusTag Fraction Plant assay with Setaria viridis; 10 days of inoculation with plant; outgrowth in LB remove 4,321,022 + +1.2 4,321,022 + -2.1 4,321,030 - -0.5 4,321,030 - +0.5 4,321,052 + -0.1 4,321,057 + +1.7 4,321,057 + +0.3 4,321,064 - +0.4 4,321,065 - -0.9 4,321,065 - +1.0 4,321,074 - -2.1 4,321,076 - -0.1 4,321,079 - -0.2 4,321,092 - -0.2 4,321,099 - -0.4 4,321,120 - -0.9 4,321,435 - HSERO_RS18835 0.26 -0.3 4,321,444 - HSERO_RS18835 0.28 -1.5 4,321,445 + HSERO_RS18835 0.28 +0.5 4,321,455 - HSERO_RS18835 0.30 -0.2 4,321,458 - HSERO_RS18835 0.30 +1.6 4,321,496 + HSERO_RS18835 0.37 -0.5 4,321,496 + HSERO_RS18835 0.37 -0.0 4,321,829 + -0.0 4,321,884 + -0.1 4,321,900 + -2.0 4,321,944 - -0.3 4,321,954 - -1.2 4,322,189 - HSERO_RS18840 0.25 +0.1 4,322,319 - HSERO_RS18840 0.41 +1.6 4,322,319 - HSERO_RS18840 0.41 -0.5 4,322,325 - HSERO_RS18840 0.42 +1.0 4,322,327 + HSERO_RS18840 0.42 +1.3 4,322,418 + HSERO_RS18840 0.54 +0.1 4,322,421 - HSERO_RS18840 0.54 -0.9 4,322,426 - HSERO_RS18840 0.55 +0.6 4,322,451 + HSERO_RS18840 0.58 -0.9 4,322,522 + HSERO_RS18840 0.67 +0.4 4,322,531 + HSERO_RS18840 0.68 -0.3 4,322,639 - HSERO_RS18840 0.82 +0.3 4,322,707 + -0.5 4,322,744 + +0.2 4,322,788 - -0.7 4,322,788 - -0.4 4,322,918 - +1.2 4,322,920 + -0.3 4,322,920 + -2.0 4,322,934 + -0.3 4,323,096 + HSERO_RS18845 0.38 +0.2 4,323,101 + HSERO_RS18845 0.39 -0.9 4,323,140 - HSERO_RS18845 0.46 +0.4 4,323,177 + +1.1 4,323,453 + -1.5 4,323,516 + -0.9 4,323,571 + HSERO_RS18850 0.14 +0.9 4,323,731 + HSERO_RS18850 0.37 -0.7
Or see this region's nucleotide sequence