Experiment: Plant assay with Setaria viridis; 10 days of inoculation with plant; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS16845 and HSERO_RS16850 are separated by 166 nucleotides HSERO_RS16850 and HSERO_RS16855 are separated by 15 nucleotides HSERO_RS16855 and HSERO_RS16860 are separated by 1 nucleotides HSERO_RS16860 and HSERO_RS16865 are separated by 61 nucleotides HSERO_RS16865 and HSERO_RS16870 are separated by 17 nucleotides
HSERO_RS16845: HSERO_RS16845 - orotidine 5'-phosphate decarboxylase, at 3,856,320 to 3,857,138
_RS16845
HSERO_RS16850: HSERO_RS16850 - lipoprotein, at 3,857,305 to 3,857,898
_RS16850
HSERO_RS16855: HSERO_RS16855 - membrane protein, at 3,857,914 to 3,858,771
_RS16855
HSERO_RS16860: HSERO_RS16860 - hypothetical protein, at 3,858,773 to 3,859,306
_RS16860
HSERO_RS16865: HSERO_RS16865 - hypothetical protein, at 3,859,368 to 3,859,607
_RS16865
HSERO_RS16870: HSERO_RS16870 - D-alanyl-D-alanine carboxypeptidase, at 3,859,625 to 3,861,031
_RS16870
Position (kb) 3857
3858
3859 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 3857.103 kb on + strand at 3857.180 kb on + strand at 3857.252 kb on + strand at 3857.280 kb on + strand at 3857.329 kb on + strand at 3857.405 kb on - strand, within HSERO_RS16850 at 3857.421 kb on + strand, within HSERO_RS16850 at 3857.449 kb on - strand, within HSERO_RS16850 at 3857.486 kb on - strand, within HSERO_RS16850 at 3857.512 kb on - strand, within HSERO_RS16850 at 3857.728 kb on + strand, within HSERO_RS16850 at 3857.822 kb on - strand, within HSERO_RS16850 at 3857.970 kb on - strand at 3858.167 kb on - strand, within HSERO_RS16855 at 3858.289 kb on + strand, within HSERO_RS16855 at 3858.289 kb on + strand, within HSERO_RS16855 at 3858.409 kb on - strand, within HSERO_RS16855 at 3858.450 kb on - strand, within HSERO_RS16855 at 3858.452 kb on + strand at 3858.462 kb on + strand, within HSERO_RS16855 at 3858.781 kb on + strand at 3858.876 kb on + strand, within HSERO_RS16860 at 3858.879 kb on - strand, within HSERO_RS16860 at 3859.057 kb on - strand, within HSERO_RS16860 at 3859.106 kb on + strand, within HSERO_RS16860 at 3859.308 kb on - strand at 3859.357 kb on - strand at 3859.357 kb on - strand at 3859.444 kb on + strand, within HSERO_RS16865 at 3859.636 kb on + strand at 3859.709 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant assay with Setaria viridis; 10 days of inoculation with plant; outgrowth in LB remove 3,857,103 + -3.1 3,857,180 + +1.1 3,857,252 + -0.3 3,857,280 + +0.6 3,857,329 + +2.0 3,857,405 - HSERO_RS16850 0.17 +0.4 3,857,421 + HSERO_RS16850 0.20 +0.4 3,857,449 - HSERO_RS16850 0.24 -0.2 3,857,486 - HSERO_RS16850 0.30 -0.4 3,857,512 - HSERO_RS16850 0.35 -0.9 3,857,728 + HSERO_RS16850 0.71 -0.2 3,857,822 - HSERO_RS16850 0.87 +1.0 3,857,970 - +0.5 3,858,167 - HSERO_RS16855 0.29 +3.3 3,858,289 + HSERO_RS16855 0.44 +1.4 3,858,289 + HSERO_RS16855 0.44 +1.1 3,858,409 - HSERO_RS16855 0.58 -0.3 3,858,450 - HSERO_RS16855 0.62 -0.1 3,858,452 + +0.7 3,858,462 + HSERO_RS16855 0.64 -0.3 3,858,781 + +0.2 3,858,876 + HSERO_RS16860 0.19 -0.0 3,858,879 - HSERO_RS16860 0.20 +0.3 3,859,057 - HSERO_RS16860 0.53 +1.0 3,859,106 + HSERO_RS16860 0.62 +0.8 3,859,308 - +0.4 3,859,357 - -0.5 3,859,357 - +0.3 3,859,444 + HSERO_RS16865 0.32 +2.6 3,859,636 + +1.3 3,859,709 - +0.0
Or see this region's nucleotide sequence