Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS18270 and BBR_RS21040 are separated by 170 nucleotides BBR_RS21040 and BBR_RS18275 overlap by 10 nucleotides BBR_RS18275 and BBR_RS18280 overlap by 4 nucleotides
BBR_RS18270: BBR_RS18270 - IS21 family transposase, at 1,884,393 to 1,885,874
_RS18270
BBR_RS21040: BBR_RS21040 - transcription factor WhiB, at 1,886,045 to 1,886,260
_RS21040
BBR_RS18275: BBR_RS18275 - ParA family protein, at 1,886,251 to 1,886,850
_RS18275
BBR_RS18280: BBR_RS18280 - hypothetical protein, at 1,886,847 to 1,887,098
_RS18280
Position (kb)
1886
1887 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1886.010 kb on + strand at 1886.045 kb on + strand at 1886.046 kb on + strand at 1886.047 kb on + strand at 1886.053 kb on - strand at 1886.053 kb on - strand at 1886.057 kb on + strand at 1886.057 kb on + strand at 1886.057 kb on + strand at 1886.057 kb on + strand at 1886.057 kb on + strand at 1886.057 kb on + strand at 1886.057 kb on + strand at 1886.062 kb on + strand at 1886.065 kb on - strand at 1886.065 kb on - strand at 1886.065 kb on - strand at 1886.065 kb on - strand at 1886.076 kb on - strand, within BBR_RS21040 at 1886.119 kb on + strand, within BBR_RS21040 at 1886.143 kb on - strand, within BBR_RS21040 at 1886.148 kb on + strand, within BBR_RS21040 at 1886.172 kb on - strand, within BBR_RS21040 at 1886.260 kb on + strand at 1886.268 kb on - strand at 1886.277 kb on + strand at 1886.286 kb on + strand at 1886.328 kb on - strand, within BBR_RS18275 at 1886.336 kb on + strand, within BBR_RS18275 at 1886.370 kb on + strand, within BBR_RS18275 at 1886.375 kb on + strand, within BBR_RS18275 at 1886.375 kb on + strand, within BBR_RS18275 at 1886.378 kb on - strand, within BBR_RS18275 at 1886.381 kb on + strand, within BBR_RS18275 at 1886.389 kb on - strand, within BBR_RS18275 at 1886.389 kb on - strand, within BBR_RS18275 at 1886.415 kb on + strand, within BBR_RS18275 at 1886.423 kb on + strand, within BBR_RS18275 at 1886.500 kb on + strand, within BBR_RS18275 at 1886.507 kb on + strand, within BBR_RS18275 at 1886.507 kb on + strand, within BBR_RS18275 at 1886.520 kb on - strand, within BBR_RS18275 at 1886.520 kb on - strand, within BBR_RS18275 at 1886.526 kb on + strand, within BBR_RS18275 at 1886.526 kb on + strand, within BBR_RS18275 at 1886.535 kb on + strand, within BBR_RS18275 at 1886.608 kb on + strand, within BBR_RS18275 at 1886.609 kb on + strand, within BBR_RS18275 at 1886.609 kb on + strand, within BBR_RS18275 at 1886.641 kb on + strand, within BBR_RS18275 at 1886.644 kb on - strand, within BBR_RS18275 at 1886.665 kb on - strand, within BBR_RS18275 at 1886.676 kb on + strand, within BBR_RS18275 at 1886.678 kb on + strand, within BBR_RS18275 at 1886.684 kb on - strand, within BBR_RS18275 at 1886.690 kb on - strand, within BBR_RS18275 at 1886.691 kb on - strand, within BBR_RS18275 at 1886.691 kb on - strand, within BBR_RS18275 at 1886.692 kb on - strand, within BBR_RS18275 at 1886.707 kb on - strand, within BBR_RS18275 at 1886.714 kb on + strand, within BBR_RS18275 at 1886.722 kb on + strand, within BBR_RS18275 at 1886.747 kb on - strand, within BBR_RS18275 at 1886.752 kb on - strand, within BBR_RS18275 at 1886.752 kb on - strand, within BBR_RS18275 at 1886.754 kb on + strand, within BBR_RS18275 at 1886.754 kb on + strand, within BBR_RS18275 at 1886.770 kb on - strand, within BBR_RS18275 at 1886.779 kb on - strand, within BBR_RS18275 at 1886.779 kb on - strand, within BBR_RS18275 at 1886.786 kb on - strand, within BBR_RS18275 at 1886.798 kb on + strand at 1886.806 kb on - strand at 1886.858 kb on + strand at 1886.858 kb on + strand at 1886.870 kb on + strand at 1886.870 kb on + strand at 1886.870 kb on + strand at 1886.870 kb on + strand at 1886.870 kb on + strand at 1886.878 kb on - strand, within BBR_RS18280 at 1886.878 kb on - strand, within BBR_RS18280 at 1886.878 kb on - strand, within BBR_RS18280 at 1886.878 kb on - strand, within BBR_RS18280 at 1886.878 kb on - strand, within BBR_RS18280 at 1886.878 kb on - strand, within BBR_RS18280 at 1886.883 kb on - strand, within BBR_RS18280 at 1886.885 kb on - strand, within BBR_RS18280 at 1886.931 kb on + strand, within BBR_RS18280 at 1886.931 kb on + strand, within BBR_RS18280 at 1886.968 kb on + strand, within BBR_RS18280 at 1886.968 kb on + strand, within BBR_RS18280 at 1886.972 kb on + strand, within BBR_RS18280 at 1886.976 kb on - strand, within BBR_RS18280 at 1886.976 kb on - strand, within BBR_RS18280 at 1886.976 kb on - strand, within BBR_RS18280 at 1887.015 kb on + strand, within BBR_RS18280 at 1887.038 kb on - strand, within BBR_RS18280 at 1887.040 kb on + strand, within BBR_RS18280 at 1887.040 kb on + strand, within BBR_RS18280 at 1887.049 kb on - strand, within BBR_RS18280 at 1887.049 kb on - strand, within BBR_RS18280 at 1887.079 kb on + strand at 1887.101 kb on - strand at 1887.101 kb on - strand at 1887.101 kb on - strand at 1887.105 kb on - strand at 1887.113 kb on + strand at 1887.211 kb on + strand at 1887.211 kb on + strand at 1887.213 kb on + strand at 1887.213 kb on + strand at 1887.213 kb on + strand at 1887.220 kb on + strand at 1887.228 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 1,886,010 + +2.5 1,886,045 + +2.7 1,886,046 + +3.7 1,886,047 + +3.2 1,886,053 - +1.1 1,886,053 - +0.1 1,886,057 + +3.5 1,886,057 + +2.8 1,886,057 + +3.2 1,886,057 + +3.3 1,886,057 + +3.9 1,886,057 + +2.5 1,886,057 + +3.3 1,886,062 + +3.0 1,886,065 - +0.9 1,886,065 - +0.4 1,886,065 - +1.0 1,886,065 - +0.7 1,886,076 - BBR_RS21040 0.14 +0.9 1,886,119 + BBR_RS21040 0.34 +3.9 1,886,143 - BBR_RS21040 0.45 +1.5 1,886,148 + BBR_RS21040 0.48 +3.3 1,886,172 - BBR_RS21040 0.59 +1.1 1,886,260 + +1.2 1,886,268 - -0.3 1,886,277 + +1.5 1,886,286 + -1.7 1,886,328 - BBR_RS18275 0.13 +0.8 1,886,336 + BBR_RS18275 0.14 +1.2 1,886,370 + BBR_RS18275 0.20 +3.0 1,886,375 + BBR_RS18275 0.21 +1.4 1,886,375 + BBR_RS18275 0.21 +1.4 1,886,378 - BBR_RS18275 0.21 -1.4 1,886,381 + BBR_RS18275 0.22 +1.7 1,886,389 - BBR_RS18275 0.23 +1.0 1,886,389 - BBR_RS18275 0.23 +0.5 1,886,415 + BBR_RS18275 0.27 +1.6 1,886,423 + BBR_RS18275 0.29 +1.1 1,886,500 + BBR_RS18275 0.41 +0.7 1,886,507 + BBR_RS18275 0.43 +1.1 1,886,507 + BBR_RS18275 0.43 +2.0 1,886,520 - BBR_RS18275 0.45 -1.4 1,886,520 - BBR_RS18275 0.45 -0.0 1,886,526 + BBR_RS18275 0.46 +2.2 1,886,526 + BBR_RS18275 0.46 +2.1 1,886,535 + BBR_RS18275 0.47 +1.4 1,886,608 + BBR_RS18275 0.59 +1.2 1,886,609 + BBR_RS18275 0.60 +2.2 1,886,609 + BBR_RS18275 0.60 +1.3 1,886,641 + BBR_RS18275 0.65 +1.3 1,886,644 - BBR_RS18275 0.66 +0.2 1,886,665 - BBR_RS18275 0.69 -1.3 1,886,676 + BBR_RS18275 0.71 +2.7 1,886,678 + BBR_RS18275 0.71 +3.9 1,886,684 - BBR_RS18275 0.72 +0.0 1,886,690 - BBR_RS18275 0.73 +1.5 1,886,691 - BBR_RS18275 0.73 -0.9 1,886,691 - BBR_RS18275 0.73 -0.7 1,886,692 - BBR_RS18275 0.73 -0.4 1,886,707 - BBR_RS18275 0.76 -0.1 1,886,714 + BBR_RS18275 0.77 +1.6 1,886,722 + BBR_RS18275 0.79 +2.5 1,886,747 - BBR_RS18275 0.83 -0.3 1,886,752 - BBR_RS18275 0.83 -0.2 1,886,752 - BBR_RS18275 0.83 +0.5 1,886,754 + BBR_RS18275 0.84 +1.9 1,886,754 + BBR_RS18275 0.84 +2.5 1,886,770 - BBR_RS18275 0.86 -0.0 1,886,779 - BBR_RS18275 0.88 -2.4 1,886,779 - BBR_RS18275 0.88 -0.6 1,886,786 - BBR_RS18275 0.89 +0.2 1,886,798 + +2.5 1,886,806 - -0.2 1,886,858 + +1.5 1,886,858 + +0.6 1,886,870 + +2.2 1,886,870 + +2.5 1,886,870 + +0.6 1,886,870 + +1.0 1,886,870 + +2.3 1,886,878 - BBR_RS18280 0.12 -1.9 1,886,878 - BBR_RS18280 0.12 -0.0 1,886,878 - BBR_RS18280 0.12 -1.2 1,886,878 - BBR_RS18280 0.12 -1.0 1,886,878 - BBR_RS18280 0.12 +0.3 1,886,878 - BBR_RS18280 0.12 +0.1 1,886,883 - BBR_RS18280 0.14 -1.2 1,886,885 - BBR_RS18280 0.15 -1.4 1,886,931 + BBR_RS18280 0.33 +2.0 1,886,931 + BBR_RS18280 0.33 +1.6 1,886,968 + BBR_RS18280 0.48 +1.9 1,886,968 + BBR_RS18280 0.48 +0.9 1,886,972 + BBR_RS18280 0.50 +0.3 1,886,976 - BBR_RS18280 0.51 +0.0 1,886,976 - BBR_RS18280 0.51 +0.9 1,886,976 - BBR_RS18280 0.51 -0.4 1,887,015 + BBR_RS18280 0.67 +2.3 1,887,038 - BBR_RS18280 0.76 -1.0 1,887,040 + BBR_RS18280 0.77 +2.0 1,887,040 + BBR_RS18280 0.77 +2.6 1,887,049 - BBR_RS18280 0.80 +0.3 1,887,049 - BBR_RS18280 0.80 -0.2 1,887,079 + +2.7 1,887,101 - +0.5 1,887,101 - +0.2 1,887,101 - +1.3 1,887,105 - +1.3 1,887,113 + +2.4 1,887,211 + +3.4 1,887,211 + -1.4 1,887,213 + +0.6 1,887,213 + +2.2 1,887,213 + -1.3 1,887,220 + +0.5 1,887,228 + -2.1
Or see this region's nucleotide sequence