Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS17745 and BBR_RS21015 are separated by 21 nucleotides BBR_RS21015 and BBR_RS17750 are separated by 285 nucleotides
BBR_RS17745: BBR_RS17745 - isoleucine--tRNA ligase, at 1,761,043 to 1,764,351
_RS17745
BBR_RS21015: BBR_RS21015 - hypothetical protein, at 1,764,373 to 1,764,738
_RS21015
BBR_RS17750: BBR_RS17750 - ribokinase, at 1,765,024 to 1,766,025
_RS17750
Position (kb)
1764
1765 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1764.373 kb on - strand at 1764.381 kb on - strand at 1764.521 kb on - strand, within BBR_RS21015 at 1764.684 kb on - strand, within BBR_RS21015 at 1764.727 kb on + strand at 1764.745 kb on + strand at 1764.767 kb on - strand at 1764.767 kb on - strand at 1764.796 kb on + strand at 1764.804 kb on + strand at 1764.804 kb on + strand at 1764.804 kb on - strand at 1764.811 kb on - strand at 1764.819 kb on - strand at 1764.821 kb on - strand at 1764.858 kb on - strand at 1764.858 kb on - strand at 1764.928 kb on + strand at 1764.928 kb on + strand at 1764.928 kb on + strand at 1764.930 kb on + strand at 1764.930 kb on + strand at 1764.936 kb on - strand at 1764.938 kb on - strand at 1764.938 kb on - strand at 1764.940 kb on + strand at 1764.943 kb on - strand at 1764.948 kb on - strand at 1764.950 kb on + strand at 1764.962 kb on + strand at 1765.001 kb on + strand at 1765.001 kb on + strand at 1765.001 kb on + strand at 1765.008 kb on + strand at 1765.008 kb on + strand at 1765.009 kb on - strand at 1765.009 kb on - strand at 1765.009 kb on - strand at 1765.016 kb on - strand at 1765.016 kb on - strand at 1765.088 kb on - strand at 1765.088 kb on - strand at 1765.110 kb on + strand at 1765.118 kb on - strand at 1765.125 kb on - strand, within BBR_RS17750 at 1765.125 kb on - strand, within BBR_RS17750 at 1765.125 kb on - strand, within BBR_RS17750 at 1765.141 kb on + strand, within BBR_RS17750 at 1765.149 kb on - strand, within BBR_RS17750 at 1765.149 kb on - strand, within BBR_RS17750 at 1765.170 kb on - strand, within BBR_RS17750 at 1765.217 kb on + strand, within BBR_RS17750 at 1765.218 kb on - strand, within BBR_RS17750 at 1765.260 kb on + strand, within BBR_RS17750 at 1765.296 kb on + strand, within BBR_RS17750 at 1765.329 kb on + strand, within BBR_RS17750 at 1765.329 kb on + strand, within BBR_RS17750 at 1765.337 kb on - strand, within BBR_RS17750 at 1765.339 kb on + strand, within BBR_RS17750 at 1765.347 kb on - strand, within BBR_RS17750 at 1765.347 kb on - strand, within BBR_RS17750 at 1765.352 kb on - strand, within BBR_RS17750 at 1765.357 kb on - strand, within BBR_RS17750 at 1765.437 kb on + strand, within BBR_RS17750 at 1765.452 kb on - strand, within BBR_RS17750 at 1765.457 kb on - strand, within BBR_RS17750 at 1765.499 kb on + strand, within BBR_RS17750 at 1765.540 kb on + strand, within BBR_RS17750 at 1765.549 kb on - strand, within BBR_RS17750 at 1765.549 kb on - strand, within BBR_RS17750 at 1765.549 kb on - strand, within BBR_RS17750 at 1765.586 kb on - strand, within BBR_RS17750 at 1765.617 kb on - strand, within BBR_RS17750 at 1765.720 kb on - strand, within BBR_RS17750 at 1765.722 kb on + strand, within BBR_RS17750 at 1765.730 kb on - strand, within BBR_RS17750
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 1,764,373 - -1.6 1,764,381 - -1.9 1,764,521 - BBR_RS21015 0.40 -3.3 1,764,684 - BBR_RS21015 0.85 -0.5 1,764,727 + +0.6 1,764,745 + +0.6 1,764,767 - -0.3 1,764,767 - +0.7 1,764,796 + +1.0 1,764,804 + -0.6 1,764,804 + +1.4 1,764,804 - -0.5 1,764,811 - +1.1 1,764,819 - +0.6 1,764,821 - -1.3 1,764,858 - +0.7 1,764,858 - -0.5 1,764,928 + -0.5 1,764,928 + +0.9 1,764,928 + +0.1 1,764,930 + -0.0 1,764,930 + +1.7 1,764,936 - +0.6 1,764,938 - +0.6 1,764,938 - -0.1 1,764,940 + -0.3 1,764,943 - +0.0 1,764,948 - +0.1 1,764,950 + -0.3 1,764,962 + +0.0 1,765,001 + +0.3 1,765,001 + +0.3 1,765,001 + +0.0 1,765,008 + +0.1 1,765,008 + +0.3 1,765,009 - +0.5 1,765,009 - +0.8 1,765,009 - +0.4 1,765,016 - -0.2 1,765,016 - +0.0 1,765,088 - +0.6 1,765,088 - +0.0 1,765,110 + -0.2 1,765,118 - +0.2 1,765,125 - BBR_RS17750 0.10 +0.4 1,765,125 - BBR_RS17750 0.10 -0.8 1,765,125 - BBR_RS17750 0.10 +0.4 1,765,141 + BBR_RS17750 0.12 +0.4 1,765,149 - BBR_RS17750 0.12 +0.2 1,765,149 - BBR_RS17750 0.12 -0.5 1,765,170 - BBR_RS17750 0.15 +0.1 1,765,217 + BBR_RS17750 0.19 -0.3 1,765,218 - BBR_RS17750 0.19 -0.8 1,765,260 + BBR_RS17750 0.24 +0.2 1,765,296 + BBR_RS17750 0.27 +0.9 1,765,329 + BBR_RS17750 0.30 +0.3 1,765,329 + BBR_RS17750 0.30 -0.1 1,765,337 - BBR_RS17750 0.31 -0.4 1,765,339 + BBR_RS17750 0.31 -0.7 1,765,347 - BBR_RS17750 0.32 +0.7 1,765,347 - BBR_RS17750 0.32 -0.3 1,765,352 - BBR_RS17750 0.33 +0.2 1,765,357 - BBR_RS17750 0.33 +0.7 1,765,437 + BBR_RS17750 0.41 -0.9 1,765,452 - BBR_RS17750 0.43 +0.6 1,765,457 - BBR_RS17750 0.43 -0.9 1,765,499 + BBR_RS17750 0.47 +0.6 1,765,540 + BBR_RS17750 0.51 -1.0 1,765,549 - BBR_RS17750 0.52 +0.1 1,765,549 - BBR_RS17750 0.52 -0.2 1,765,549 - BBR_RS17750 0.52 +1.5 1,765,586 - BBR_RS17750 0.56 -0.5 1,765,617 - BBR_RS17750 0.59 +0.6 1,765,720 - BBR_RS17750 0.69 +0.5 1,765,722 + BBR_RS17750 0.70 +0.4 1,765,730 - BBR_RS17750 0.70 +0.5
Or see this region's nucleotide sequence