Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14355 and BBR_RS14360 are separated by 316 nucleotides BBR_RS14360 and BBR_RS20825 overlap by 29 nucleotides BBR_RS20825 and BBR_RS14365 overlap by 2 nucleotides
BBR_RS14355: BBR_RS14355 - tRNA-Pro, at 995,115 to 995,191
_RS14355
BBR_RS14360: BBR_RS14360 - hypothetical protein, at 995,508 to 996,182
_RS14360
BBR_RS20825: BBR_RS20825 - hypothetical protein, at 996,154 to 996,345
_RS20825
BBR_RS14365: BBR_RS14365 - hypothetical protein, at 996,344 to 997,516
_RS14365
Position (kb) 996
997 Strain fitness (log2 ratio) -1
0
1 at 995.375 kb on - strand at 995.439 kb on + strand at 995.484 kb on + strand at 995.492 kb on + strand at 995.556 kb on - strand at 995.558 kb on + strand at 995.599 kb on - strand, within BBR_RS14360 at 995.654 kb on - strand, within BBR_RS14360 at 995.764 kb on + strand, within BBR_RS14360 at 995.764 kb on + strand, within BBR_RS14360 at 995.801 kb on + strand, within BBR_RS14360 at 995.846 kb on + strand, within BBR_RS14360 at 995.854 kb on - strand, within BBR_RS14360 at 995.854 kb on - strand, within BBR_RS14360 at 995.890 kb on + strand, within BBR_RS14360 at 995.981 kb on + strand, within BBR_RS14360 at 995.989 kb on - strand, within BBR_RS14360 at 995.989 kb on - strand, within BBR_RS14360 at 995.989 kb on - strand, within BBR_RS14360 at 995.989 kb on - strand, within BBR_RS14360 at 995.989 kb on - strand, within BBR_RS14360 at 995.989 kb on - strand, within BBR_RS14360 at 996.002 kb on - strand, within BBR_RS14360 at 996.014 kb on + strand, within BBR_RS14360 at 996.023 kb on - strand, within BBR_RS14360 at 996.040 kb on + strand, within BBR_RS14360 at 996.045 kb on + strand, within BBR_RS14360 at 996.066 kb on - strand, within BBR_RS14360 at 996.080 kb on - strand, within BBR_RS14360 at 996.143 kb on - strand at 996.244 kb on + strand, within BBR_RS20825 at 996.244 kb on + strand, within BBR_RS20825 at 996.344 kb on - strand at 996.432 kb on - strand at 996.481 kb on + strand, within BBR_RS14365 at 996.491 kb on - strand, within BBR_RS14365 at 996.507 kb on + strand, within BBR_RS14365 at 996.527 kb on + strand, within BBR_RS14365 at 996.565 kb on + strand, within BBR_RS14365 at 996.578 kb on - strand, within BBR_RS14365 at 996.616 kb on + strand, within BBR_RS14365 at 996.616 kb on + strand, within BBR_RS14365 at 996.730 kb on - strand, within BBR_RS14365 at 996.742 kb on + strand, within BBR_RS14365 at 996.833 kb on + strand, within BBR_RS14365 at 996.848 kb on + strand, within BBR_RS14365 at 996.928 kb on + strand, within BBR_RS14365 at 996.932 kb on - strand, within BBR_RS14365 at 997.011 kb on + strand, within BBR_RS14365 at 997.021 kb on + strand, within BBR_RS14365 at 997.021 kb on + strand, within BBR_RS14365 at 997.029 kb on - strand, within BBR_RS14365 at 997.143 kb on - strand, within BBR_RS14365 at 997.150 kb on - strand, within BBR_RS14365 at 997.150 kb on - strand, within BBR_RS14365 at 997.293 kb on + strand, within BBR_RS14365 at 997.344 kb on - strand, within BBR_RS14365
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 995,375 - +0.5 995,439 + -0.0 995,484 + +0.1 995,492 + -0.4 995,556 - -0.4 995,558 + -0.1 995,599 - BBR_RS14360 0.13 +0.8 995,654 - BBR_RS14360 0.22 -0.7 995,764 + BBR_RS14360 0.38 -0.0 995,764 + BBR_RS14360 0.38 -0.6 995,801 + BBR_RS14360 0.43 +0.2 995,846 + BBR_RS14360 0.50 +0.5 995,854 - BBR_RS14360 0.51 -0.0 995,854 - BBR_RS14360 0.51 +0.2 995,890 + BBR_RS14360 0.57 -0.3 995,981 + BBR_RS14360 0.70 -0.5 995,989 - BBR_RS14360 0.71 +0.2 995,989 - BBR_RS14360 0.71 +0.1 995,989 - BBR_RS14360 0.71 -1.6 995,989 - BBR_RS14360 0.71 +0.5 995,989 - BBR_RS14360 0.71 +0.1 995,989 - BBR_RS14360 0.71 -0.1 996,002 - BBR_RS14360 0.73 +0.0 996,014 + BBR_RS14360 0.75 -0.6 996,023 - BBR_RS14360 0.76 -0.6 996,040 + BBR_RS14360 0.79 +0.0 996,045 + BBR_RS14360 0.80 +0.3 996,066 - BBR_RS14360 0.83 +0.2 996,080 - BBR_RS14360 0.85 +0.0 996,143 - +0.0 996,244 + BBR_RS20825 0.47 -0.6 996,244 + BBR_RS20825 0.47 -0.4 996,344 - +0.9 996,432 - -0.2 996,481 + BBR_RS14365 0.12 +0.1 996,491 - BBR_RS14365 0.13 +0.2 996,507 + BBR_RS14365 0.14 +1.5 996,527 + BBR_RS14365 0.16 +0.5 996,565 + BBR_RS14365 0.19 +0.7 996,578 - BBR_RS14365 0.20 -0.8 996,616 + BBR_RS14365 0.23 -0.2 996,616 + BBR_RS14365 0.23 -1.3 996,730 - BBR_RS14365 0.33 +0.5 996,742 + BBR_RS14365 0.34 -0.2 996,833 + BBR_RS14365 0.42 +0.1 996,848 + BBR_RS14365 0.43 +0.3 996,928 + BBR_RS14365 0.50 -1.1 996,932 - BBR_RS14365 0.50 -0.0 997,011 + BBR_RS14365 0.57 +1.1 997,021 + BBR_RS14365 0.58 +0.2 997,021 + BBR_RS14365 0.58 +0.2 997,029 - BBR_RS14365 0.58 -1.1 997,143 - BBR_RS14365 0.68 +1.2 997,150 - BBR_RS14365 0.69 +0.4 997,150 - BBR_RS14365 0.69 +0.8 997,293 + BBR_RS14365 0.81 +1.5 997,344 - BBR_RS14365 0.85 -0.0
Or see this region's nucleotide sequence