Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12235 and BBR_RS12240 are separated by 129 nucleotides BBR_RS12240 and BBR_RS20685 overlap by 40 nucleotides BBR_RS20685 and BBR_RS12245 are separated by 40 nucleotides
BBR_RS12235: BBR_RS12235 - hypothetical protein, at 494,728 to 495,414
_RS12235
BBR_RS12240: BBR_RS12240 - 30S ribosomal protein S15, at 495,544 to 495,813
_RS12240
BBR_RS20685: BBR_RS20685 - hypothetical protein, at 495,774 to 496,049
_RS20685
BBR_RS12245: BBR_RS12245 - polyribonucleotide nucleotidyltransferase, at 496,090 to 498,801
_RS12245
Position (kb) 495
496
497 Strain fitness (log2 ratio) -2
-1
0
1 at 494.787 kb on + strand at 494.787 kb on + strand at 494.871 kb on + strand, within BBR_RS12235 at 495.834 kb on + strand, within BBR_RS20685 at 495.840 kb on + strand, within BBR_RS20685 at 495.845 kb on + strand, within BBR_RS20685 at 495.845 kb on + strand, within BBR_RS20685 at 495.845 kb on + strand, within BBR_RS20685 at 495.892 kb on + strand, within BBR_RS20685 at 495.893 kb on + strand, within BBR_RS20685 at 495.893 kb on + strand, within BBR_RS20685 at 495.911 kb on + strand, within BBR_RS20685 at 495.927 kb on - strand, within BBR_RS20685 at 495.941 kb on - strand, within BBR_RS20685 at 495.988 kb on + strand, within BBR_RS20685 at 496.029 kb on + strand at 496.031 kb on + strand at 496.416 kb on - strand, within BBR_RS12245
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 494,787 + +0.4 494,787 + -2.6 494,871 + BBR_RS12235 0.21 -2.5 495,834 + BBR_RS20685 0.22 -0.2 495,840 + BBR_RS20685 0.24 -1.0 495,845 + BBR_RS20685 0.26 -0.3 495,845 + BBR_RS20685 0.26 -1.5 495,845 + BBR_RS20685 0.26 -1.4 495,892 + BBR_RS20685 0.43 +0.2 495,893 + BBR_RS20685 0.43 -0.4 495,893 + BBR_RS20685 0.43 -0.9 495,911 + BBR_RS20685 0.50 -2.0 495,927 - BBR_RS20685 0.55 +1.4 495,941 - BBR_RS20685 0.61 +1.5 495,988 + BBR_RS20685 0.78 -0.1 496,029 + -1.9 496,031 + -0.3 496,416 - BBR_RS12245 0.12 +0.7
Or see this region's nucleotide sequence