Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS19755 and BBR_RS19760 are separated by 24 nucleotides BBR_RS19760 and BBR_RS19765 overlap by 1 nucleotides BBR_RS19765 and BBR_RS19770 are separated by 21 nucleotides
BBR_RS19755: BBR_RS19755 - glycosyl transferase, at 2,222,180 to 2,223,703
_RS19755
BBR_RS19760: BBR_RS19760 - polysaccharide ABC transporter ATPase, at 2,223,728 to 2,224,996
_RS19760
BBR_RS19765: BBR_RS19765 - ABC transporter permease, at 2,224,996 to 2,225,829
_RS19765
BBR_RS19770: BBR_RS19770 - alpha-L-Rha alpha-1,2-L-rhamnosyltransferase, at 2,225,851 to 2,227,851
_RS19770
Position (kb)
2223
2224
2225 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2222.736 kb on + strand, within BBR_RS19755 at 2222.744 kb on - strand, within BBR_RS19755 at 2222.746 kb on + strand, within BBR_RS19755 at 2222.746 kb on - strand, within BBR_RS19755 at 2222.756 kb on - strand, within BBR_RS19755 at 2222.761 kb on - strand, within BBR_RS19755 at 2222.772 kb on - strand, within BBR_RS19755 at 2222.786 kb on - strand, within BBR_RS19755 at 2222.786 kb on - strand, within BBR_RS19755 at 2222.786 kb on - strand, within BBR_RS19755 at 2222.786 kb on - strand, within BBR_RS19755 at 2222.788 kb on + strand, within BBR_RS19755 at 2222.788 kb on + strand, within BBR_RS19755 at 2222.788 kb on + strand, within BBR_RS19755 at 2222.796 kb on - strand, within BBR_RS19755 at 2222.866 kb on + strand, within BBR_RS19755 at 2222.868 kb on + strand, within BBR_RS19755 at 2222.876 kb on + strand, within BBR_RS19755 at 2222.876 kb on + strand, within BBR_RS19755 at 2222.876 kb on + strand, within BBR_RS19755 at 2222.878 kb on - strand, within BBR_RS19755 at 2222.895 kb on - strand, within BBR_RS19755 at 2222.911 kb on + strand, within BBR_RS19755 at 2222.949 kb on - strand, within BBR_RS19755 at 2222.957 kb on - strand, within BBR_RS19755 at 2223.010 kb on - strand, within BBR_RS19755 at 2223.010 kb on - strand, within BBR_RS19755 at 2223.018 kb on - strand, within BBR_RS19755 at 2223.030 kb on - strand, within BBR_RS19755 at 2223.033 kb on + strand, within BBR_RS19755 at 2223.033 kb on - strand, within BBR_RS19755 at 2223.041 kb on - strand, within BBR_RS19755 at 2223.067 kb on - strand, within BBR_RS19755 at 2223.074 kb on + strand, within BBR_RS19755 at 2223.085 kb on + strand, within BBR_RS19755 at 2223.110 kb on - strand, within BBR_RS19755 at 2223.122 kb on + strand, within BBR_RS19755 at 2223.130 kb on - strand, within BBR_RS19755 at 2223.156 kb on - strand, within BBR_RS19755 at 2223.197 kb on + strand, within BBR_RS19755 at 2223.197 kb on - strand, within BBR_RS19755 at 2223.205 kb on + strand, within BBR_RS19755 at 2223.214 kb on - strand, within BBR_RS19755 at 2223.215 kb on - strand, within BBR_RS19755 at 2223.217 kb on + strand, within BBR_RS19755 at 2223.217 kb on + strand, within BBR_RS19755 at 2223.223 kb on + strand, within BBR_RS19755 at 2223.229 kb on + strand, within BBR_RS19755 at 2223.229 kb on + strand, within BBR_RS19755 at 2223.237 kb on + strand, within BBR_RS19755 at 2223.242 kb on + strand, within BBR_RS19755 at 2223.244 kb on - strand, within BBR_RS19755 at 2223.264 kb on + strand, within BBR_RS19755 at 2223.275 kb on - strand, within BBR_RS19755 at 2223.275 kb on - strand, within BBR_RS19755 at 2223.294 kb on - strand, within BBR_RS19755 at 2223.294 kb on - strand, within BBR_RS19755 at 2223.300 kb on + strand, within BBR_RS19755 at 2223.337 kb on - strand, within BBR_RS19755 at 2223.381 kb on - strand, within BBR_RS19755 at 2223.391 kb on - strand, within BBR_RS19755 at 2223.391 kb on - strand, within BBR_RS19755 at 2223.403 kb on + strand, within BBR_RS19755 at 2223.406 kb on - strand, within BBR_RS19755 at 2223.411 kb on - strand, within BBR_RS19755 at 2223.416 kb on - strand, within BBR_RS19755 at 2223.463 kb on - strand, within BBR_RS19755 at 2223.483 kb on + strand, within BBR_RS19755 at 2223.491 kb on - strand, within BBR_RS19755 at 2223.491 kb on - strand, within BBR_RS19755 at 2223.493 kb on + strand, within BBR_RS19755 at 2223.493 kb on - strand, within BBR_RS19755 at 2223.541 kb on + strand, within BBR_RS19755 at 2223.566 kb on - strand at 2223.570 kb on - strand at 2223.589 kb on + strand at 2223.694 kb on - strand at 2223.703 kb on + strand at 2223.705 kb on - strand at 2223.706 kb on - strand at 2223.718 kb on - strand at 2223.739 kb on - strand at 2223.975 kb on - strand, within BBR_RS19760 at 2224.101 kb on - strand, within BBR_RS19760 at 2224.140 kb on - strand, within BBR_RS19760 at 2224.140 kb on - strand, within BBR_RS19760 at 2224.964 kb on - strand at 2224.964 kb on - strand at 2224.964 kb on - strand at 2224.964 kb on - strand at 2224.979 kb on - strand at 2224.984 kb on - strand at 2224.989 kb on - strand at 2225.872 kb on - strand at 2225.895 kb on - strand at 2225.895 kb on - strand at 2225.910 kb on - strand at 2225.910 kb on - strand at 2225.928 kb on - strand at 2225.940 kb on - strand at 2225.940 kb on - strand at 2225.941 kb on - strand at 2225.949 kb on - strand at 2225.951 kb on - strand at 2225.954 kb on - strand at 2225.954 kb on - strand at 2225.963 kb on - strand at 2225.965 kb on - strand at 2225.965 kb on - strand at 2225.970 kb on - strand at 2225.975 kb on - strand at 2225.975 kb on - strand at 2225.975 kb on - strand at 2225.980 kb on - strand at 2225.980 kb on - strand at 2225.980 kb on - strand at 2225.991 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 2,222,736 + BBR_RS19755 0.36 -0.1 2,222,744 - BBR_RS19755 0.37 -0.7 2,222,746 + BBR_RS19755 0.37 -0.1 2,222,746 - BBR_RS19755 0.37 +1.6 2,222,756 - BBR_RS19755 0.38 +0.5 2,222,761 - BBR_RS19755 0.38 +0.7 2,222,772 - BBR_RS19755 0.39 -2.1 2,222,786 - BBR_RS19755 0.40 -0.7 2,222,786 - BBR_RS19755 0.40 -1.9 2,222,786 - BBR_RS19755 0.40 +1.0 2,222,786 - BBR_RS19755 0.40 +0.2 2,222,788 + BBR_RS19755 0.40 -0.5 2,222,788 + BBR_RS19755 0.40 +0.3 2,222,788 + BBR_RS19755 0.40 -0.2 2,222,796 - BBR_RS19755 0.40 +0.5 2,222,866 + BBR_RS19755 0.45 -0.2 2,222,868 + BBR_RS19755 0.45 -2.3 2,222,876 + BBR_RS19755 0.46 +1.1 2,222,876 + BBR_RS19755 0.46 +1.1 2,222,876 + BBR_RS19755 0.46 -1.2 2,222,878 - BBR_RS19755 0.46 -1.4 2,222,895 - BBR_RS19755 0.47 -0.6 2,222,911 + BBR_RS19755 0.48 -0.3 2,222,949 - BBR_RS19755 0.50 +2.0 2,222,957 - BBR_RS19755 0.51 +0.1 2,223,010 - BBR_RS19755 0.54 -0.5 2,223,010 - BBR_RS19755 0.54 +0.3 2,223,018 - BBR_RS19755 0.55 -1.3 2,223,030 - BBR_RS19755 0.56 -1.3 2,223,033 + BBR_RS19755 0.56 +0.7 2,223,033 - BBR_RS19755 0.56 +0.1 2,223,041 - BBR_RS19755 0.56 -0.3 2,223,067 - BBR_RS19755 0.58 +0.2 2,223,074 + BBR_RS19755 0.59 +1.3 2,223,085 + BBR_RS19755 0.59 -1.2 2,223,110 - BBR_RS19755 0.61 +2.6 2,223,122 + BBR_RS19755 0.62 +1.0 2,223,130 - BBR_RS19755 0.62 -0.6 2,223,156 - BBR_RS19755 0.64 +2.0 2,223,197 + BBR_RS19755 0.67 +0.7 2,223,197 - BBR_RS19755 0.67 +1.0 2,223,205 + BBR_RS19755 0.67 +1.2 2,223,214 - BBR_RS19755 0.68 -1.2 2,223,215 - BBR_RS19755 0.68 -0.9 2,223,217 + BBR_RS19755 0.68 +1.0 2,223,217 + BBR_RS19755 0.68 -0.2 2,223,223 + BBR_RS19755 0.68 +1.7 2,223,229 + BBR_RS19755 0.69 +1.1 2,223,229 + BBR_RS19755 0.69 -1.2 2,223,237 + BBR_RS19755 0.69 +1.4 2,223,242 + BBR_RS19755 0.70 +2.0 2,223,244 - BBR_RS19755 0.70 +1.4 2,223,264 + BBR_RS19755 0.71 +0.8 2,223,275 - BBR_RS19755 0.72 -0.6 2,223,275 - BBR_RS19755 0.72 -2.3 2,223,294 - BBR_RS19755 0.73 +1.5 2,223,294 - BBR_RS19755 0.73 +0.7 2,223,300 + BBR_RS19755 0.73 -0.3 2,223,337 - BBR_RS19755 0.76 -1.2 2,223,381 - BBR_RS19755 0.79 +0.7 2,223,391 - BBR_RS19755 0.79 +0.7 2,223,391 - BBR_RS19755 0.79 -0.6 2,223,403 + BBR_RS19755 0.80 +1.8 2,223,406 - BBR_RS19755 0.80 +0.7 2,223,411 - BBR_RS19755 0.81 +0.6 2,223,416 - BBR_RS19755 0.81 +1.4 2,223,463 - BBR_RS19755 0.84 -0.2 2,223,483 + BBR_RS19755 0.85 +1.0 2,223,491 - BBR_RS19755 0.86 -1.3 2,223,491 - BBR_RS19755 0.86 -0.5 2,223,493 + BBR_RS19755 0.86 +2.3 2,223,493 - BBR_RS19755 0.86 -0.9 2,223,541 + BBR_RS19755 0.89 +1.0 2,223,566 - -0.7 2,223,570 - +0.8 2,223,589 + +2.0 2,223,694 - -1.7 2,223,703 + -0.9 2,223,705 - +1.5 2,223,706 - -0.1 2,223,718 - -0.3 2,223,739 - +1.4 2,223,975 - BBR_RS19760 0.19 -1.9 2,224,101 - BBR_RS19760 0.29 +1.0 2,224,140 - BBR_RS19760 0.32 -0.2 2,224,140 - BBR_RS19760 0.32 +0.4 2,224,964 - -2.7 2,224,964 - +0.2 2,224,964 - +0.5 2,224,964 - -1.2 2,224,979 - -1.2 2,224,984 - -3.4 2,224,989 - -0.5 2,225,872 - -0.5 2,225,895 - -0.0 2,225,895 - +1.0 2,225,910 - +2.0 2,225,910 - -0.5 2,225,928 - -1.5 2,225,940 - +1.2 2,225,940 - +0.0 2,225,941 - -0.9 2,225,949 - -1.5 2,225,951 - +1.4 2,225,954 - -0.3 2,225,954 - -0.5 2,225,963 - -0.8 2,225,965 - +1.0 2,225,965 - +0.4 2,225,970 - -1.1 2,225,975 - +0.0 2,225,975 - -0.7 2,225,975 - -0.6 2,225,980 - +1.4 2,225,980 - +0.7 2,225,980 - -0.8 2,225,991 - +0.7
Or see this region's nucleotide sequence