Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS19445 and BBR_RS19450 are separated by 106 nucleotides BBR_RS19450 and BBR_RS19455 are separated by 66 nucleotides BBR_RS19455 and BBR_RS19460 are separated by 22 nucleotides BBR_RS19460 and BBR_RS19465 are separated by 186 nucleotides
BBR_RS19445: BBR_RS19445 - glutamate 5-kinase, at 2,145,028 to 2,146,161
_RS19445
BBR_RS19450: BBR_RS19450 - GTPase ObgE, at 2,146,268 to 2,147,959
_RS19450
BBR_RS19455: BBR_RS19455 - 50S ribosomal protein L27, at 2,148,026 to 2,148,277
_RS19455
BBR_RS19460: BBR_RS19460 - 50S ribosomal protein L21, at 2,148,300 to 2,148,608
_RS19460
BBR_RS19465: BBR_RS19465 - ribonuclease E/G, at 2,148,795 to 2,151,857
_RS19465
Position (kb)
2146
2147
2148 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2145.271 kb on + strand, within BBR_RS19445 at 2145.271 kb on + strand, within BBR_RS19445 at 2145.277 kb on + strand, within BBR_RS19445 at 2145.288 kb on + strand, within BBR_RS19445 at 2145.292 kb on + strand, within BBR_RS19445 at 2145.297 kb on + strand, within BBR_RS19445 at 2145.305 kb on - strand, within BBR_RS19445 at 2145.320 kb on - strand, within BBR_RS19445 at 2145.332 kb on - strand, within BBR_RS19445 at 2145.334 kb on - strand, within BBR_RS19445 at 2145.347 kb on + strand, within BBR_RS19445 at 2145.355 kb on + strand, within BBR_RS19445 at 2145.355 kb on - strand, within BBR_RS19445 at 2145.356 kb on + strand, within BBR_RS19445 at 2145.356 kb on + strand, within BBR_RS19445 at 2145.364 kb on - strand, within BBR_RS19445 at 2145.364 kb on - strand, within BBR_RS19445 at 2145.366 kb on + strand, within BBR_RS19445 at 2145.366 kb on + strand, within BBR_RS19445 at 2145.375 kb on - strand, within BBR_RS19445 at 2145.381 kb on - strand, within BBR_RS19445 at 2145.396 kb on + strand, within BBR_RS19445 at 2145.417 kb on + strand, within BBR_RS19445 at 2145.432 kb on - strand, within BBR_RS19445 at 2145.465 kb on - strand, within BBR_RS19445 at 2145.467 kb on + strand, within BBR_RS19445 at 2145.475 kb on - strand, within BBR_RS19445 at 2145.475 kb on - strand, within BBR_RS19445 at 2145.520 kb on + strand, within BBR_RS19445 at 2145.530 kb on - strand, within BBR_RS19445 at 2145.530 kb on - strand, within BBR_RS19445 at 2145.531 kb on + strand, within BBR_RS19445 at 2145.540 kb on - strand, within BBR_RS19445 at 2145.540 kb on - strand, within BBR_RS19445 at 2145.548 kb on + strand, within BBR_RS19445 at 2145.553 kb on + strand, within BBR_RS19445 at 2145.556 kb on + strand, within BBR_RS19445 at 2145.558 kb on + strand, within BBR_RS19445 at 2145.571 kb on + strand, within BBR_RS19445 at 2145.588 kb on - strand, within BBR_RS19445 at 2145.588 kb on - strand, within BBR_RS19445 at 2145.588 kb on - strand, within BBR_RS19445 at 2145.594 kb on + strand, within BBR_RS19445 at 2145.594 kb on + strand, within BBR_RS19445 at 2145.596 kb on + strand, within BBR_RS19445 at 2145.596 kb on + strand, within BBR_RS19445 at 2145.596 kb on + strand, within BBR_RS19445 at 2145.596 kb on + strand, within BBR_RS19445 at 2145.596 kb on + strand, within BBR_RS19445 at 2145.598 kb on + strand, within BBR_RS19445 at 2145.598 kb on + strand, within BBR_RS19445 at 2145.599 kb on + strand, within BBR_RS19445 at 2145.601 kb on + strand, within BBR_RS19445 at 2145.602 kb on - strand, within BBR_RS19445 at 2145.607 kb on - strand, within BBR_RS19445 at 2145.607 kb on - strand, within BBR_RS19445 at 2145.607 kb on - strand, within BBR_RS19445 at 2145.609 kb on - strand, within BBR_RS19445 at 2145.609 kb on - strand, within BBR_RS19445 at 2145.609 kb on - strand, within BBR_RS19445 at 2145.609 kb on - strand, within BBR_RS19445 at 2145.628 kb on + strand, within BBR_RS19445 at 2145.636 kb on + strand, within BBR_RS19445 at 2145.654 kb on - strand, within BBR_RS19445 at 2145.660 kb on + strand, within BBR_RS19445 at 2145.662 kb on + strand, within BBR_RS19445 at 2145.741 kb on + strand, within BBR_RS19445 at 2145.743 kb on - strand, within BBR_RS19445 at 2145.749 kb on - strand, within BBR_RS19445 at 2145.772 kb on - strand, within BBR_RS19445 at 2145.772 kb on - strand, within BBR_RS19445 at 2145.784 kb on + strand, within BBR_RS19445 at 2145.784 kb on + strand, within BBR_RS19445 at 2145.784 kb on + strand, within BBR_RS19445 at 2145.784 kb on + strand, within BBR_RS19445 at 2145.789 kb on + strand, within BBR_RS19445 at 2145.792 kb on - strand, within BBR_RS19445 at 2145.792 kb on - strand, within BBR_RS19445 at 2145.817 kb on + strand, within BBR_RS19445 at 2145.817 kb on + strand, within BBR_RS19445 at 2145.825 kb on - strand, within BBR_RS19445 at 2145.826 kb on - strand, within BBR_RS19445 at 2145.834 kb on + strand, within BBR_RS19445 at 2145.834 kb on + strand, within BBR_RS19445 at 2145.850 kb on + strand, within BBR_RS19445 at 2145.850 kb on + strand, within BBR_RS19445 at 2145.850 kb on + strand, within BBR_RS19445 at 2145.861 kb on + strand, within BBR_RS19445 at 2145.868 kb on - strand, within BBR_RS19445 at 2145.868 kb on - strand, within BBR_RS19445 at 2145.883 kb on + strand, within BBR_RS19445 at 2145.883 kb on + strand, within BBR_RS19445 at 2145.891 kb on - strand, within BBR_RS19445 at 2145.907 kb on - strand, within BBR_RS19445 at 2145.912 kb on - strand, within BBR_RS19445 at 2145.915 kb on - strand, within BBR_RS19445 at 2145.944 kb on - strand, within BBR_RS19445 at 2145.972 kb on + strand, within BBR_RS19445 at 2145.982 kb on + strand, within BBR_RS19445 at 2145.982 kb on + strand, within BBR_RS19445 at 2145.982 kb on + strand, within BBR_RS19445 at 2145.982 kb on + strand, within BBR_RS19445 at 2145.987 kb on + strand, within BBR_RS19445 at 2145.990 kb on + strand, within BBR_RS19445 at 2145.995 kb on - strand, within BBR_RS19445 at 2145.997 kb on + strand, within BBR_RS19445 at 2146.001 kb on + strand, within BBR_RS19445 at 2146.009 kb on - strand, within BBR_RS19445 at 2146.009 kb on - strand, within BBR_RS19445 at 2146.016 kb on - strand, within BBR_RS19445 at 2146.030 kb on + strand, within BBR_RS19445 at 2146.037 kb on + strand, within BBR_RS19445 at 2146.060 kb on - strand at 2146.064 kb on + strand at 2146.088 kb on + strand at 2146.096 kb on - strand at 2146.096 kb on - strand at 2146.096 kb on - strand at 2146.107 kb on - strand at 2146.107 kb on - strand at 2146.124 kb on + strand at 2146.137 kb on - strand at 2146.146 kb on + strand at 2146.146 kb on + strand at 2146.154 kb on - strand at 2146.162 kb on - strand at 2146.217 kb on - strand at 2146.238 kb on + strand at 2146.238 kb on + strand at 2146.241 kb on - strand at 2146.241 kb on - strand at 2146.241 kb on - strand at 2146.241 kb on - strand at 2146.241 kb on - strand at 2146.243 kb on + strand at 2146.243 kb on - strand at 2146.243 kb on - strand at 2146.251 kb on - strand at 2146.327 kb on - strand at 2146.359 kb on - strand at 2146.364 kb on - strand at 2146.368 kb on + strand at 2146.368 kb on + strand at 2146.368 kb on + strand at 2146.368 kb on + strand at 2146.368 kb on + strand at 2146.373 kb on + strand at 2146.376 kb on - strand at 2146.376 kb on - strand at 2146.376 kb on - strand at 2146.376 kb on - strand at 2146.376 kb on - strand at 2146.388 kb on - strand at 2146.394 kb on + strand at 2146.545 kb on - strand, within BBR_RS19450 at 2146.856 kb on - strand, within BBR_RS19450 at 2146.880 kb on - strand, within BBR_RS19450 at 2148.017 kb on - strand at 2148.017 kb on - strand at 2148.701 kb on + strand at 2148.723 kb on - strand at 2148.723 kb on - strand at 2148.734 kb on + strand at 2148.764 kb on + strand at 2148.772 kb on - strand at 2148.787 kb on + strand at 2148.787 kb on + strand at 2148.787 kb on + strand at 2148.795 kb on - strand at 2148.800 kb on + strand at 2148.833 kb on + strand at 2148.889 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 2,145,271 + BBR_RS19445 0.21 -0.1 2,145,271 + BBR_RS19445 0.21 +0.3 2,145,277 + BBR_RS19445 0.22 -0.1 2,145,288 + BBR_RS19445 0.23 -1.0 2,145,292 + BBR_RS19445 0.23 +1.1 2,145,297 + BBR_RS19445 0.24 -1.0 2,145,305 - BBR_RS19445 0.24 -1.1 2,145,320 - BBR_RS19445 0.26 -0.3 2,145,332 - BBR_RS19445 0.27 -1.4 2,145,334 - BBR_RS19445 0.27 +0.3 2,145,347 + BBR_RS19445 0.28 +0.4 2,145,355 + BBR_RS19445 0.29 -0.2 2,145,355 - BBR_RS19445 0.29 -0.6 2,145,356 + BBR_RS19445 0.29 -0.8 2,145,356 + BBR_RS19445 0.29 -0.3 2,145,364 - BBR_RS19445 0.30 +0.4 2,145,364 - BBR_RS19445 0.30 -0.7 2,145,366 + BBR_RS19445 0.30 -0.9 2,145,366 + BBR_RS19445 0.30 -0.6 2,145,375 - BBR_RS19445 0.31 -1.3 2,145,381 - BBR_RS19445 0.31 -0.5 2,145,396 + BBR_RS19445 0.32 -0.0 2,145,417 + BBR_RS19445 0.34 -1.0 2,145,432 - BBR_RS19445 0.36 -1.2 2,145,465 - BBR_RS19445 0.39 -0.8 2,145,467 + BBR_RS19445 0.39 +0.2 2,145,475 - BBR_RS19445 0.39 +0.7 2,145,475 - BBR_RS19445 0.39 +0.5 2,145,520 + BBR_RS19445 0.43 +0.3 2,145,530 - BBR_RS19445 0.44 -0.4 2,145,530 - BBR_RS19445 0.44 +0.1 2,145,531 + BBR_RS19445 0.44 +0.7 2,145,540 - BBR_RS19445 0.45 +0.9 2,145,540 - BBR_RS19445 0.45 -0.5 2,145,548 + BBR_RS19445 0.46 +0.6 2,145,553 + BBR_RS19445 0.46 +0.9 2,145,556 + BBR_RS19445 0.47 -0.8 2,145,558 + BBR_RS19445 0.47 -0.5 2,145,571 + BBR_RS19445 0.48 -0.9 2,145,588 - BBR_RS19445 0.49 -1.8 2,145,588 - BBR_RS19445 0.49 -0.4 2,145,588 - BBR_RS19445 0.49 -1.3 2,145,594 + BBR_RS19445 0.50 +0.7 2,145,594 + BBR_RS19445 0.50 -0.0 2,145,596 + BBR_RS19445 0.50 -0.3 2,145,596 + BBR_RS19445 0.50 +0.5 2,145,596 + BBR_RS19445 0.50 -0.3 2,145,596 + BBR_RS19445 0.50 -0.2 2,145,596 + BBR_RS19445 0.50 +0.3 2,145,598 + BBR_RS19445 0.50 +0.1 2,145,598 + BBR_RS19445 0.50 +0.3 2,145,599 + BBR_RS19445 0.50 +0.1 2,145,601 + BBR_RS19445 0.51 +0.7 2,145,602 - BBR_RS19445 0.51 -0.1 2,145,607 - BBR_RS19445 0.51 +0.6 2,145,607 - BBR_RS19445 0.51 +0.3 2,145,607 - BBR_RS19445 0.51 -0.4 2,145,609 - BBR_RS19445 0.51 +0.1 2,145,609 - BBR_RS19445 0.51 -0.9 2,145,609 - BBR_RS19445 0.51 +0.3 2,145,609 - BBR_RS19445 0.51 +0.1 2,145,628 + BBR_RS19445 0.53 +0.3 2,145,636 + BBR_RS19445 0.54 +0.1 2,145,654 - BBR_RS19445 0.55 +0.6 2,145,660 + BBR_RS19445 0.56 +0.7 2,145,662 + BBR_RS19445 0.56 -0.0 2,145,741 + BBR_RS19445 0.63 -1.1 2,145,743 - BBR_RS19445 0.63 -2.8 2,145,749 - BBR_RS19445 0.64 -0.1 2,145,772 - BBR_RS19445 0.66 +0.3 2,145,772 - BBR_RS19445 0.66 -0.3 2,145,784 + BBR_RS19445 0.67 +0.1 2,145,784 + BBR_RS19445 0.67 -0.5 2,145,784 + BBR_RS19445 0.67 -0.0 2,145,784 + BBR_RS19445 0.67 -0.6 2,145,789 + BBR_RS19445 0.67 -1.1 2,145,792 - BBR_RS19445 0.67 +1.0 2,145,792 - BBR_RS19445 0.67 +0.9 2,145,817 + BBR_RS19445 0.70 -0.3 2,145,817 + BBR_RS19445 0.70 -0.8 2,145,825 - BBR_RS19445 0.70 +0.2 2,145,826 - BBR_RS19445 0.70 +0.9 2,145,834 + BBR_RS19445 0.71 +0.3 2,145,834 + BBR_RS19445 0.71 +0.8 2,145,850 + BBR_RS19445 0.72 -1.1 2,145,850 + BBR_RS19445 0.72 -0.9 2,145,850 + BBR_RS19445 0.72 -0.1 2,145,861 + BBR_RS19445 0.73 +0.6 2,145,868 - BBR_RS19445 0.74 -1.9 2,145,868 - BBR_RS19445 0.74 +1.7 2,145,883 + BBR_RS19445 0.75 -1.2 2,145,883 + BBR_RS19445 0.75 -1.4 2,145,891 - BBR_RS19445 0.76 +0.8 2,145,907 - BBR_RS19445 0.78 -0.6 2,145,912 - BBR_RS19445 0.78 +0.2 2,145,915 - BBR_RS19445 0.78 -0.3 2,145,944 - BBR_RS19445 0.81 +0.5 2,145,972 + BBR_RS19445 0.83 +1.1 2,145,982 + BBR_RS19445 0.84 +0.4 2,145,982 + BBR_RS19445 0.84 -0.5 2,145,982 + BBR_RS19445 0.84 +0.8 2,145,982 + BBR_RS19445 0.84 -2.4 2,145,987 + BBR_RS19445 0.85 -0.0 2,145,990 + BBR_RS19445 0.85 +0.5 2,145,995 - BBR_RS19445 0.85 +0.4 2,145,997 + BBR_RS19445 0.85 -0.3 2,146,001 + BBR_RS19445 0.86 +0.5 2,146,009 - BBR_RS19445 0.87 +0.3 2,146,009 - BBR_RS19445 0.87 -0.6 2,146,016 - BBR_RS19445 0.87 -1.4 2,146,030 + BBR_RS19445 0.88 -0.2 2,146,037 + BBR_RS19445 0.89 -0.3 2,146,060 - -0.4 2,146,064 + -0.8 2,146,088 + -0.3 2,146,096 - -0.1 2,146,096 - -0.3 2,146,096 - -0.7 2,146,107 - +0.6 2,146,107 - -0.7 2,146,124 + -0.8 2,146,137 - -0.1 2,146,146 + +0.2 2,146,146 + +0.6 2,146,154 - -1.4 2,146,162 - +0.5 2,146,217 - -0.2 2,146,238 + -1.6 2,146,238 + +0.2 2,146,241 - -1.2 2,146,241 - -0.4 2,146,241 - +0.6 2,146,241 - -0.9 2,146,241 - +0.8 2,146,243 + +0.3 2,146,243 - +0.1 2,146,243 - -0.6 2,146,251 - -0.4 2,146,327 - -0.4 2,146,359 - -0.6 2,146,364 - -0.7 2,146,368 + -0.2 2,146,368 + +1.2 2,146,368 + +1.4 2,146,368 + +0.3 2,146,368 + -0.3 2,146,373 + +0.1 2,146,376 - +0.4 2,146,376 - +0.4 2,146,376 - +0.4 2,146,376 - -0.4 2,146,376 - +0.9 2,146,388 - +2.3 2,146,394 + +2.2 2,146,545 - BBR_RS19450 0.16 +1.4 2,146,856 - BBR_RS19450 0.35 +2.0 2,146,880 - BBR_RS19450 0.36 -1.1 2,148,017 - -0.3 2,148,017 - -1.1 2,148,701 + +0.3 2,148,723 - +0.1 2,148,723 - -0.1 2,148,734 + -0.5 2,148,764 + +0.6 2,148,772 - -0.9 2,148,787 + +1.0 2,148,787 + +0.9 2,148,787 + -0.2 2,148,795 - +1.3 2,148,800 + +0.5 2,148,833 + +0.1 2,148,889 - -1.4
Or see this region's nucleotide sequence