Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS19285 and BBR_RS19290 are separated by 1 nucleotides BBR_RS19290 and BBR_RS19295 are separated by 335 nucleotides
BBR_RS19285: BBR_RS19285 - tRNA (guanosine(37)-N1)-methyltransferase TrmD, at 2,104,933 to 2,105,883
_RS19285
BBR_RS19290: BBR_RS19290 - 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD, at 2,105,885 to 2,106,466
_RS19290
BBR_RS19295: BBR_RS19295 - ATP-dependent DNA helicase RecG, at 2,106,802 to 2,109,408
_RS19295
Position (kb)
2105
2106
2107 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2104.890 kb on - strand at 2104.890 kb on - strand at 2104.890 kb on - strand at 2104.890 kb on - strand at 2104.890 kb on - strand at 2104.892 kb on - strand at 2104.894 kb on - strand at 2104.894 kb on - strand at 2104.896 kb on - strand at 2104.910 kb on + strand at 2104.910 kb on + strand at 2104.910 kb on + strand at 2104.910 kb on + strand at 2104.910 kb on + strand at 2104.916 kb on + strand at 2104.918 kb on - strand at 2104.918 kb on - strand at 2104.921 kb on + strand at 2104.924 kb on - strand at 2104.924 kb on - strand at 2105.829 kb on + strand at 2105.843 kb on + strand at 2105.849 kb on - strand at 2105.851 kb on + strand at 2105.851 kb on - strand at 2105.873 kb on + strand at 2105.877 kb on + strand at 2105.881 kb on - strand at 2105.883 kb on + strand at 2105.891 kb on - strand at 2105.903 kb on - strand at 2105.905 kb on + strand at 2105.910 kb on + strand at 2105.913 kb on - strand at 2105.913 kb on - strand at 2105.913 kb on - strand at 2105.918 kb on - strand at 2105.918 kb on - strand at 2105.960 kb on + strand, within BBR_RS19290 at 2105.960 kb on + strand, within BBR_RS19290 at 2105.964 kb on + strand, within BBR_RS19290 at 2106.020 kb on + strand, within BBR_RS19290 at 2106.025 kb on - strand, within BBR_RS19290 at 2106.055 kb on + strand, within BBR_RS19290 at 2106.055 kb on + strand, within BBR_RS19290 at 2106.058 kb on - strand, within BBR_RS19290 at 2106.078 kb on + strand, within BBR_RS19290 at 2106.078 kb on + strand, within BBR_RS19290 at 2106.078 kb on - strand, within BBR_RS19290 at 2106.084 kb on + strand, within BBR_RS19290 at 2106.092 kb on - strand, within BBR_RS19290 at 2106.092 kb on - strand, within BBR_RS19290 at 2106.129 kb on - strand, within BBR_RS19290 at 2106.140 kb on - strand, within BBR_RS19290 at 2106.156 kb on - strand, within BBR_RS19290 at 2106.160 kb on + strand, within BBR_RS19290 at 2106.168 kb on - strand, within BBR_RS19290 at 2106.289 kb on - strand, within BBR_RS19290 at 2106.341 kb on + strand, within BBR_RS19290 at 2106.349 kb on - strand, within BBR_RS19290 at 2106.349 kb on - strand, within BBR_RS19290 at 2106.349 kb on - strand, within BBR_RS19290 at 2106.357 kb on - strand, within BBR_RS19290 at 2106.382 kb on + strand, within BBR_RS19290 at 2106.391 kb on + strand, within BBR_RS19290 at 2106.391 kb on + strand, within BBR_RS19290 at 2106.404 kb on - strand, within BBR_RS19290 at 2106.414 kb on + strand at 2106.422 kb on - strand at 2106.433 kb on - strand at 2106.438 kb on + strand at 2106.438 kb on + strand at 2106.438 kb on + strand at 2106.438 kb on + strand at 2106.438 kb on + strand at 2106.438 kb on + strand at 2106.438 kb on + strand at 2106.438 kb on + strand at 2106.438 kb on - strand at 2106.440 kb on + strand at 2106.446 kb on - strand at 2106.446 kb on - strand at 2106.446 kb on - strand at 2106.446 kb on - strand at 2106.483 kb on - strand at 2106.486 kb on + strand at 2106.494 kb on + strand at 2106.494 kb on - strand at 2106.504 kb on + strand at 2106.504 kb on + strand at 2106.504 kb on + strand at 2106.512 kb on - strand at 2106.512 kb on - strand at 2106.525 kb on + strand at 2106.525 kb on + strand at 2106.525 kb on + strand at 2106.525 kb on + strand at 2106.525 kb on + strand at 2106.572 kb on + strand at 2106.577 kb on + strand at 2106.580 kb on - strand at 2106.588 kb on + strand at 2106.603 kb on + strand at 2106.621 kb on - strand at 2106.674 kb on + strand at 2106.684 kb on + strand at 2106.690 kb on + strand at 2106.713 kb on + strand at 2106.720 kb on + strand at 2106.732 kb on + strand at 2106.742 kb on - strand at 2106.758 kb on - strand at 2106.798 kb on + strand at 2106.798 kb on + strand at 2106.798 kb on + strand at 2106.806 kb on - strand at 2106.820 kb on - strand at 2106.829 kb on + strand at 2106.838 kb on - strand at 2106.841 kb on + strand at 2106.841 kb on + strand at 2106.841 kb on + strand at 2106.841 kb on + strand at 2106.841 kb on + strand at 2106.849 kb on - strand at 2106.849 kb on - strand at 2106.849 kb on - strand at 2106.879 kb on + strand at 2106.879 kb on + strand at 2106.887 kb on - strand at 2106.887 kb on - strand at 2106.890 kb on + strand at 2106.898 kb on - strand at 2106.898 kb on - strand at 2106.903 kb on + strand at 2106.903 kb on + strand at 2106.908 kb on + strand at 2106.912 kb on + strand at 2106.912 kb on + strand at 2106.921 kb on - strand at 2106.926 kb on + strand at 2106.934 kb on - strand at 2106.960 kb on + strand at 2106.965 kb on + strand at 2106.968 kb on - strand at 2106.968 kb on - strand at 2106.970 kb on + strand at 2106.970 kb on + strand at 2106.973 kb on - strand at 2106.974 kb on - strand at 2106.977 kb on + strand at 2106.982 kb on + strand at 2106.992 kb on + strand at 2107.000 kb on - strand at 2107.005 kb on + strand at 2107.005 kb on + strand at 2107.029 kb on - strand at 2107.057 kb on - strand at 2107.104 kb on - strand, within BBR_RS19295 at 2107.124 kb on + strand, within BBR_RS19295 at 2107.147 kb on + strand, within BBR_RS19295 at 2107.149 kb on + strand, within BBR_RS19295 at 2107.157 kb on - strand, within BBR_RS19295 at 2107.157 kb on - strand, within BBR_RS19295 at 2107.157 kb on - strand, within BBR_RS19295 at 2107.176 kb on + strand, within BBR_RS19295 at 2107.182 kb on + strand, within BBR_RS19295 at 2107.182 kb on + strand, within BBR_RS19295 at 2107.182 kb on + strand, within BBR_RS19295 at 2107.182 kb on + strand, within BBR_RS19295 at 2107.184 kb on + strand, within BBR_RS19295 at 2107.185 kb on - strand, within BBR_RS19295 at 2107.185 kb on - strand, within BBR_RS19295 at 2107.190 kb on - strand, within BBR_RS19295 at 2107.191 kb on - strand, within BBR_RS19295 at 2107.194 kb on - strand, within BBR_RS19295 at 2107.249 kb on - strand, within BBR_RS19295 at 2107.256 kb on + strand, within BBR_RS19295 at 2107.256 kb on + strand, within BBR_RS19295 at 2107.266 kb on + strand, within BBR_RS19295 at 2107.298 kb on - strand, within BBR_RS19295 at 2107.319 kb on + strand, within BBR_RS19295 at 2107.323 kb on + strand, within BBR_RS19295 at 2107.327 kb on - strand, within BBR_RS19295 at 2107.378 kb on - strand, within BBR_RS19295 at 2107.417 kb on + strand, within BBR_RS19295 at 2107.428 kb on - strand, within BBR_RS19295 at 2107.428 kb on - strand, within BBR_RS19295 at 2107.434 kb on - strand, within BBR_RS19295
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 2,104,890 - +0.4 2,104,890 - -0.0 2,104,890 - -0.3 2,104,890 - +0.2 2,104,890 - +0.6 2,104,892 - +0.2 2,104,894 - +0.5 2,104,894 - -0.1 2,104,896 - +0.4 2,104,910 + -0.1 2,104,910 + +0.0 2,104,910 + -0.1 2,104,910 + -0.3 2,104,910 + +0.3 2,104,916 + -0.5 2,104,918 - -0.1 2,104,918 - +0.3 2,104,921 + +0.8 2,104,924 - +0.2 2,104,924 - +2.2 2,105,829 + -0.8 2,105,843 + -0.0 2,105,849 - -0.4 2,105,851 + +0.2 2,105,851 - +0.1 2,105,873 + -0.9 2,105,877 + +0.1 2,105,881 - +0.6 2,105,883 + -0.2 2,105,891 - +0.8 2,105,903 - +0.0 2,105,905 + -0.2 2,105,910 + +0.7 2,105,913 - -1.3 2,105,913 - -0.8 2,105,913 - -0.3 2,105,918 - -0.6 2,105,918 - +0.6 2,105,960 + BBR_RS19290 0.13 -1.0 2,105,960 + BBR_RS19290 0.13 -0.1 2,105,964 + BBR_RS19290 0.14 -0.3 2,106,020 + BBR_RS19290 0.23 -1.1 2,106,025 - BBR_RS19290 0.24 -0.9 2,106,055 + BBR_RS19290 0.29 -0.4 2,106,055 + BBR_RS19290 0.29 +0.6 2,106,058 - BBR_RS19290 0.30 -0.4 2,106,078 + BBR_RS19290 0.33 -0.3 2,106,078 + BBR_RS19290 0.33 +1.0 2,106,078 - BBR_RS19290 0.33 -0.3 2,106,084 + BBR_RS19290 0.34 -2.2 2,106,092 - BBR_RS19290 0.36 +0.4 2,106,092 - BBR_RS19290 0.36 -0.2 2,106,129 - BBR_RS19290 0.42 -0.8 2,106,140 - BBR_RS19290 0.44 -1.5 2,106,156 - BBR_RS19290 0.47 -0.9 2,106,160 + BBR_RS19290 0.47 -0.6 2,106,168 - BBR_RS19290 0.49 -1.4 2,106,289 - BBR_RS19290 0.69 -1.5 2,106,341 + BBR_RS19290 0.78 -0.7 2,106,349 - BBR_RS19290 0.80 -0.5 2,106,349 - BBR_RS19290 0.80 -0.3 2,106,349 - BBR_RS19290 0.80 -0.7 2,106,357 - BBR_RS19290 0.81 -0.8 2,106,382 + BBR_RS19290 0.85 +1.0 2,106,391 + BBR_RS19290 0.87 +0.9 2,106,391 + BBR_RS19290 0.87 -1.1 2,106,404 - BBR_RS19290 0.89 +0.6 2,106,414 + +2.0 2,106,422 - -1.4 2,106,433 - -1.8 2,106,438 + +0.3 2,106,438 + +0.3 2,106,438 + -0.7 2,106,438 + -0.8 2,106,438 + +0.3 2,106,438 + +0.4 2,106,438 + -0.6 2,106,438 + +1.1 2,106,438 - -0.8 2,106,440 + -0.2 2,106,446 - +0.4 2,106,446 - -1.4 2,106,446 - -1.7 2,106,446 - -0.9 2,106,483 - +0.4 2,106,486 + -0.3 2,106,494 + +0.2 2,106,494 - -0.4 2,106,504 + -0.5 2,106,504 + -1.0 2,106,504 + -0.4 2,106,512 - +1.3 2,106,512 - -0.1 2,106,525 + -0.7 2,106,525 + -0.3 2,106,525 + +0.5 2,106,525 + +1.0 2,106,525 + -0.1 2,106,572 + -0.8 2,106,577 + -0.8 2,106,580 - -0.5 2,106,588 + +0.1 2,106,603 + -1.5 2,106,621 - +1.9 2,106,674 + -0.8 2,106,684 + -0.6 2,106,690 + -1.1 2,106,713 + -0.9 2,106,720 + +0.4 2,106,732 + +1.2 2,106,742 - -0.6 2,106,758 - -0.6 2,106,798 + +0.2 2,106,798 + +0.5 2,106,798 + +0.7 2,106,806 - -0.6 2,106,820 - +0.0 2,106,829 + +0.7 2,106,838 - -0.1 2,106,841 + -1.6 2,106,841 + +0.1 2,106,841 + -2.0 2,106,841 + -0.2 2,106,841 + -0.3 2,106,849 - +0.7 2,106,849 - +0.2 2,106,849 - +1.5 2,106,879 + +0.6 2,106,879 + -0.1 2,106,887 - -0.1 2,106,887 - +1.1 2,106,890 + -0.6 2,106,898 - -0.9 2,106,898 - -1.1 2,106,903 + +0.3 2,106,903 + +0.1 2,106,908 + -0.1 2,106,912 + -1.4 2,106,912 + -0.4 2,106,921 - -0.4 2,106,926 + -0.1 2,106,934 - -0.7 2,106,960 + -0.8 2,106,965 + +0.2 2,106,968 - -0.9 2,106,968 - +0.3 2,106,970 + -0.4 2,106,970 + -0.7 2,106,973 - -1.1 2,106,974 - -1.7 2,106,977 + -0.2 2,106,982 + -0.1 2,106,992 + -0.3 2,107,000 - -2.7 2,107,005 + -0.2 2,107,005 + -0.4 2,107,029 - -0.4 2,107,057 - +0.7 2,107,104 - BBR_RS19295 0.12 +0.2 2,107,124 + BBR_RS19295 0.12 -1.1 2,107,147 + BBR_RS19295 0.13 -1.1 2,107,149 + BBR_RS19295 0.13 +0.5 2,107,157 - BBR_RS19295 0.14 +0.7 2,107,157 - BBR_RS19295 0.14 +1.4 2,107,157 - BBR_RS19295 0.14 -0.6 2,107,176 + BBR_RS19295 0.14 +2.2 2,107,182 + BBR_RS19295 0.15 +0.5 2,107,182 + BBR_RS19295 0.15 -1.3 2,107,182 + BBR_RS19295 0.15 -1.4 2,107,182 + BBR_RS19295 0.15 -1.2 2,107,184 + BBR_RS19295 0.15 -1.4 2,107,185 - BBR_RS19295 0.15 -1.1 2,107,185 - BBR_RS19295 0.15 -0.6 2,107,190 - BBR_RS19295 0.15 -0.0 2,107,191 - BBR_RS19295 0.15 -2.6 2,107,194 - BBR_RS19295 0.15 +0.1 2,107,249 - BBR_RS19295 0.17 -1.0 2,107,256 + BBR_RS19295 0.17 -0.1 2,107,256 + BBR_RS19295 0.17 -0.0 2,107,266 + BBR_RS19295 0.18 -0.7 2,107,298 - BBR_RS19295 0.19 -1.2 2,107,319 + BBR_RS19295 0.20 -1.3 2,107,323 + BBR_RS19295 0.20 -2.6 2,107,327 - BBR_RS19295 0.20 -0.4 2,107,378 - BBR_RS19295 0.22 -2.0 2,107,417 + BBR_RS19295 0.24 -1.1 2,107,428 - BBR_RS19295 0.24 +0.2 2,107,428 - BBR_RS19295 0.24 -0.1 2,107,434 - BBR_RS19295 0.24 +1.6
Or see this region's nucleotide sequence