Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS19105 and BBR_RS19110 are separated by 137 nucleotides BBR_RS19110 and BBR_RS19115 are separated by 239 nucleotides BBR_RS19115 and BBR_RS19120 are separated by 2 nucleotides
BBR_RS19105: BBR_RS19105 - FmdB family transcriptional regulator, at 2,060,255 to 2,060,440
_RS19105
BBR_RS19110: BBR_RS19110 - flagellar protein FlgA, at 2,060,578 to 2,061,144
_RS19110
BBR_RS19115: BBR_RS19115 - hypothetical protein, at 2,061,384 to 2,061,584
_RS19115
BBR_RS19120: BBR_RS19120 - helicase, at 2,061,587 to 2,065,207
_RS19120
Position (kb)
2061
2062 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 2060.416 kb on - strand, within BBR_RS19105 at 2060.418 kb on + strand, within BBR_RS19105 at 2060.435 kb on - strand at 2060.435 kb on - strand at 2060.466 kb on - strand at 2060.472 kb on + strand at 2060.495 kb on + strand at 2060.500 kb on + strand at 2060.508 kb on - strand at 2060.509 kb on + strand at 2060.509 kb on + strand at 2060.518 kb on - strand at 2060.524 kb on - strand at 2060.524 kb on - strand at 2060.555 kb on - strand at 2060.558 kb on + strand at 2060.558 kb on + strand at 2060.566 kb on - strand at 2060.566 kb on - strand at 2060.566 kb on - strand at 2060.566 kb on - strand at 2060.573 kb on + strand at 2060.573 kb on + strand at 2060.573 kb on + strand at 2060.573 kb on + strand at 2060.573 kb on + strand at 2060.573 kb on + strand at 2060.573 kb on + strand at 2060.573 kb on + strand at 2060.575 kb on + strand at 2060.581 kb on - strand at 2060.581 kb on - strand at 2060.605 kb on - strand at 2060.620 kb on + strand at 2060.628 kb on - strand at 2060.628 kb on - strand at 2060.650 kb on + strand, within BBR_RS19110 at 2060.650 kb on + strand, within BBR_RS19110 at 2060.650 kb on + strand, within BBR_RS19110 at 2060.650 kb on + strand, within BBR_RS19110 at 2060.653 kb on - strand, within BBR_RS19110 at 2060.658 kb on - strand, within BBR_RS19110 at 2060.661 kb on + strand, within BBR_RS19110 at 2060.667 kb on - strand, within BBR_RS19110 at 2060.667 kb on - strand, within BBR_RS19110 at 2060.669 kb on - strand, within BBR_RS19110 at 2060.669 kb on - strand, within BBR_RS19110 at 2060.669 kb on - strand, within BBR_RS19110 at 2060.670 kb on - strand, within BBR_RS19110 at 2060.672 kb on + strand, within BBR_RS19110 at 2060.676 kb on + strand, within BBR_RS19110 at 2060.676 kb on + strand, within BBR_RS19110 at 2060.701 kb on - strand, within BBR_RS19110 at 2060.701 kb on - strand, within BBR_RS19110 at 2060.739 kb on - strand, within BBR_RS19110 at 2060.751 kb on + strand, within BBR_RS19110 at 2060.751 kb on + strand, within BBR_RS19110 at 2060.753 kb on - strand, within BBR_RS19110 at 2060.759 kb on - strand, within BBR_RS19110 at 2060.759 kb on - strand, within BBR_RS19110 at 2060.764 kb on - strand, within BBR_RS19110 at 2060.764 kb on - strand, within BBR_RS19110 at 2060.769 kb on + strand, within BBR_RS19110 at 2060.769 kb on - strand, within BBR_RS19110 at 2060.777 kb on - strand, within BBR_RS19110 at 2060.777 kb on - strand, within BBR_RS19110 at 2060.777 kb on - strand, within BBR_RS19110 at 2060.793 kb on + strand, within BBR_RS19110 at 2060.795 kb on + strand, within BBR_RS19110 at 2060.795 kb on + strand, within BBR_RS19110 at 2060.822 kb on - strand, within BBR_RS19110 at 2060.833 kb on + strand, within BBR_RS19110 at 2060.843 kb on - strand, within BBR_RS19110 at 2060.851 kb on - strand, within BBR_RS19110 at 2060.859 kb on + strand, within BBR_RS19110 at 2060.859 kb on + strand, within BBR_RS19110 at 2060.867 kb on - strand, within BBR_RS19110 at 2060.867 kb on - strand, within BBR_RS19110 at 2060.885 kb on + strand, within BBR_RS19110 at 2060.932 kb on + strand, within BBR_RS19110 at 2060.936 kb on + strand, within BBR_RS19110 at 2060.939 kb on + strand, within BBR_RS19110 at 2060.940 kb on - strand, within BBR_RS19110 at 2060.942 kb on + strand, within BBR_RS19110 at 2060.965 kb on - strand, within BBR_RS19110 at 2060.967 kb on - strand, within BBR_RS19110 at 2060.974 kb on + strand, within BBR_RS19110 at 2060.977 kb on - strand, within BBR_RS19110 at 2061.006 kb on + strand, within BBR_RS19110 at 2061.009 kb on - strand, within BBR_RS19110 at 2061.009 kb on - strand, within BBR_RS19110 at 2061.022 kb on - strand, within BBR_RS19110 at 2061.022 kb on - strand, within BBR_RS19110 at 2061.024 kb on + strand, within BBR_RS19110 at 2061.032 kb on - strand, within BBR_RS19110 at 2061.050 kb on + strand, within BBR_RS19110 at 2061.060 kb on + strand, within BBR_RS19110 at 2061.081 kb on - strand, within BBR_RS19110 at 2061.095 kb on + strand at 2061.095 kb on + strand at 2061.105 kb on - strand at 2061.107 kb on - strand at 2061.117 kb on - strand at 2061.131 kb on + strand at 2061.139 kb on - strand at 2061.163 kb on + strand at 2061.187 kb on + strand at 2061.199 kb on + strand at 2061.200 kb on + strand at 2061.205 kb on - strand at 2061.205 kb on - strand at 2061.222 kb on + strand at 2061.244 kb on - strand at 2061.246 kb on - strand at 2061.248 kb on - strand at 2061.262 kb on - strand at 2061.281 kb on - strand at 2061.285 kb on + strand at 2061.285 kb on + strand at 2061.285 kb on + strand at 2061.290 kb on - strand at 2061.293 kb on - strand at 2061.293 kb on - strand at 2061.295 kb on + strand at 2061.295 kb on + strand at 2061.295 kb on + strand at 2061.297 kb on + strand at 2061.303 kb on - strand at 2061.303 kb on - strand at 2061.308 kb on - strand at 2061.313 kb on - strand at 2061.315 kb on - strand at 2061.316 kb on + strand at 2061.317 kb on - strand at 2061.319 kb on + strand at 2061.327 kb on - strand at 2061.354 kb on + strand at 2061.366 kb on + strand at 2061.366 kb on + strand at 2061.369 kb on + strand at 2061.374 kb on + strand at 2061.375 kb on - strand at 2061.377 kb on - strand at 2061.377 kb on - strand at 2061.383 kb on - strand at 2061.401 kb on + strand at 2061.404 kb on + strand, within BBR_RS19115 at 2061.406 kb on + strand, within BBR_RS19115 at 2061.412 kb on - strand, within BBR_RS19115 at 2061.414 kb on - strand, within BBR_RS19115 at 2061.435 kb on - strand, within BBR_RS19115 at 2061.438 kb on - strand, within BBR_RS19115 at 2061.456 kb on + strand, within BBR_RS19115 at 2061.477 kb on - strand, within BBR_RS19115 at 2061.491 kb on + strand, within BBR_RS19115 at 2061.499 kb on - strand, within BBR_RS19115 at 2061.562 kb on - strand, within BBR_RS19115 at 2061.569 kb on - strand at 2061.569 kb on - strand at 2061.575 kb on + strand at 2061.584 kb on + strand at 2061.584 kb on + strand at 2061.592 kb on - strand at 2061.593 kb on - strand at 2061.595 kb on - strand at 2061.595 kb on - strand at 2061.595 kb on - strand at 2061.618 kb on - strand at 2061.669 kb on - strand at 2061.676 kb on - strand at 2061.676 kb on - strand at 2061.722 kb on - strand at 2061.735 kb on + strand at 2061.799 kb on + strand at 2061.799 kb on + strand at 2061.807 kb on - strand at 2061.811 kb on + strand at 2061.811 kb on + strand at 2061.811 kb on + strand at 2061.825 kb on + strand at 2061.833 kb on - strand at 2061.859 kb on + strand at 2061.859 kb on + strand at 2061.867 kb on + strand at 2061.867 kb on - strand at 2061.867 kb on - strand at 2061.867 kb on - strand at 2061.867 kb on - strand at 2061.867 kb on - strand at 2061.867 kb on - strand at 2061.882 kb on + strand at 2061.894 kb on + strand at 2061.937 kb on + strand at 2061.963 kb on + strand, within BBR_RS19120 at 2061.963 kb on + strand, within BBR_RS19120 at 2061.966 kb on - strand, within BBR_RS19120 at 2061.966 kb on - strand, within BBR_RS19120 at 2061.968 kb on + strand, within BBR_RS19120 at 2061.971 kb on - strand, within BBR_RS19120 at 2061.981 kb on - strand, within BBR_RS19120 at 2062.028 kb on - strand, within BBR_RS19120 at 2062.028 kb on - strand, within BBR_RS19120 at 2062.099 kb on - strand, within BBR_RS19120 at 2062.128 kb on - strand, within BBR_RS19120 at 2062.282 kb on - strand, within BBR_RS19120 at 2062.309 kb on - strand, within BBR_RS19120 at 2062.313 kb on - strand, within BBR_RS19120 at 2062.374 kb on + strand, within BBR_RS19120 at 2062.374 kb on + strand, within BBR_RS19120 at 2062.382 kb on - strand, within BBR_RS19120 at 2062.397 kb on - strand, within BBR_RS19120 at 2062.398 kb on - strand, within BBR_RS19120 at 2062.422 kb on + strand, within BBR_RS19120 at 2062.430 kb on - strand, within BBR_RS19120 at 2062.446 kb on + strand, within BBR_RS19120 at 2062.454 kb on + strand, within BBR_RS19120 at 2062.454 kb on - strand, within BBR_RS19120 at 2062.482 kb on + strand, within BBR_RS19120 at 2062.499 kb on + strand, within BBR_RS19120 at 2062.512 kb on - strand, within BBR_RS19120 at 2062.517 kb on - strand, within BBR_RS19120 at 2062.566 kb on - strand, within BBR_RS19120
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 2,060,416 - BBR_RS19105 0.87 -0.1 2,060,418 + BBR_RS19105 0.88 -0.3 2,060,435 - -1.2 2,060,435 - +0.2 2,060,466 - -0.4 2,060,472 + +0.6 2,060,495 + +0.4 2,060,500 + -0.9 2,060,508 - -0.6 2,060,509 + +0.7 2,060,509 + +0.2 2,060,518 - -0.2 2,060,524 - +1.0 2,060,524 - +0.7 2,060,555 - -0.3 2,060,558 + +0.6 2,060,558 + +0.2 2,060,566 - +0.2 2,060,566 - -1.3 2,060,566 - +0.0 2,060,566 - -0.7 2,060,573 + -0.5 2,060,573 + +2.4 2,060,573 + +1.3 2,060,573 + +0.1 2,060,573 + +0.4 2,060,573 + -0.3 2,060,573 + +0.4 2,060,573 + +1.0 2,060,575 + +0.7 2,060,581 - +0.3 2,060,581 - +0.1 2,060,605 - -0.3 2,060,620 + -0.4 2,060,628 - +0.5 2,060,628 - -0.5 2,060,650 + BBR_RS19110 0.13 +0.6 2,060,650 + BBR_RS19110 0.13 +0.7 2,060,650 + BBR_RS19110 0.13 +0.8 2,060,650 + BBR_RS19110 0.13 -0.6 2,060,653 - BBR_RS19110 0.13 +0.6 2,060,658 - BBR_RS19110 0.14 -0.3 2,060,661 + BBR_RS19110 0.15 +0.0 2,060,667 - BBR_RS19110 0.16 -0.5 2,060,667 - BBR_RS19110 0.16 +0.3 2,060,669 - BBR_RS19110 0.16 +0.3 2,060,669 - BBR_RS19110 0.16 -0.5 2,060,669 - BBR_RS19110 0.16 +0.8 2,060,670 - BBR_RS19110 0.16 -0.7 2,060,672 + BBR_RS19110 0.17 +0.2 2,060,676 + BBR_RS19110 0.17 -0.3 2,060,676 + BBR_RS19110 0.17 +0.4 2,060,701 - BBR_RS19110 0.22 +0.2 2,060,701 - BBR_RS19110 0.22 -0.2 2,060,739 - BBR_RS19110 0.28 +0.4 2,060,751 + BBR_RS19110 0.31 +0.8 2,060,751 + BBR_RS19110 0.31 +0.6 2,060,753 - BBR_RS19110 0.31 -0.4 2,060,759 - BBR_RS19110 0.32 -0.2 2,060,759 - BBR_RS19110 0.32 -0.1 2,060,764 - BBR_RS19110 0.33 +0.2 2,060,764 - BBR_RS19110 0.33 -0.4 2,060,769 + BBR_RS19110 0.34 -1.2 2,060,769 - BBR_RS19110 0.34 -2.8 2,060,777 - BBR_RS19110 0.35 +0.5 2,060,777 - BBR_RS19110 0.35 -0.4 2,060,777 - BBR_RS19110 0.35 -0.2 2,060,793 + BBR_RS19110 0.38 +0.6 2,060,795 + BBR_RS19110 0.38 -1.0 2,060,795 + BBR_RS19110 0.38 -0.3 2,060,822 - BBR_RS19110 0.43 +0.7 2,060,833 + BBR_RS19110 0.45 -0.8 2,060,843 - BBR_RS19110 0.47 +0.5 2,060,851 - BBR_RS19110 0.48 +0.0 2,060,859 + BBR_RS19110 0.50 -0.7 2,060,859 + BBR_RS19110 0.50 +1.1 2,060,867 - BBR_RS19110 0.51 +0.1 2,060,867 - BBR_RS19110 0.51 +0.1 2,060,885 + BBR_RS19110 0.54 +0.7 2,060,932 + BBR_RS19110 0.62 -1.3 2,060,936 + BBR_RS19110 0.63 -0.0 2,060,939 + BBR_RS19110 0.64 +1.0 2,060,940 - BBR_RS19110 0.64 -0.7 2,060,942 + BBR_RS19110 0.64 -0.9 2,060,965 - BBR_RS19110 0.68 +0.3 2,060,967 - BBR_RS19110 0.69 -0.2 2,060,974 + BBR_RS19110 0.70 +0.4 2,060,977 - BBR_RS19110 0.70 +0.7 2,061,006 + BBR_RS19110 0.75 +0.5 2,061,009 - BBR_RS19110 0.76 +0.0 2,061,009 - BBR_RS19110 0.76 +0.2 2,061,022 - BBR_RS19110 0.78 +0.4 2,061,022 - BBR_RS19110 0.78 +0.2 2,061,024 + BBR_RS19110 0.79 -1.4 2,061,032 - BBR_RS19110 0.80 +0.8 2,061,050 + BBR_RS19110 0.83 +0.8 2,061,060 + BBR_RS19110 0.85 -0.4 2,061,081 - BBR_RS19110 0.89 +0.4 2,061,095 + -0.3 2,061,095 + +0.6 2,061,105 - +0.3 2,061,107 - -0.9 2,061,117 - +0.6 2,061,131 + +0.8 2,061,139 - -0.0 2,061,163 + -0.1 2,061,187 + -0.4 2,061,199 + +0.4 2,061,200 + -0.4 2,061,205 - +0.2 2,061,205 - +0.0 2,061,222 + +0.3 2,061,244 - -0.8 2,061,246 - -0.6 2,061,248 - +2.3 2,061,262 - +0.3 2,061,281 - -0.9 2,061,285 + -0.4 2,061,285 + -0.8 2,061,285 + +2.1 2,061,290 - +0.1 2,061,293 - -0.7 2,061,293 - +0.8 2,061,295 + +0.2 2,061,295 + -1.3 2,061,295 + +0.1 2,061,297 + +0.3 2,061,303 - -0.3 2,061,303 - +1.0 2,061,308 - -0.7 2,061,313 - +0.1 2,061,315 - +0.0 2,061,316 + +0.2 2,061,317 - +0.1 2,061,319 + +0.3 2,061,327 - +0.3 2,061,354 + +0.2 2,061,366 + -0.1 2,061,366 + +0.1 2,061,369 + -1.3 2,061,374 + -1.6 2,061,375 - +0.4 2,061,377 - +0.8 2,061,377 - -1.7 2,061,383 - +0.2 2,061,401 + +0.9 2,061,404 + BBR_RS19115 0.10 +0.5 2,061,406 + BBR_RS19115 0.11 -0.7 2,061,412 - BBR_RS19115 0.14 -1.3 2,061,414 - BBR_RS19115 0.15 -1.0 2,061,435 - BBR_RS19115 0.25 -1.8 2,061,438 - BBR_RS19115 0.27 +2.0 2,061,456 + BBR_RS19115 0.36 -2.0 2,061,477 - BBR_RS19115 0.46 -2.0 2,061,491 + BBR_RS19115 0.53 +0.2 2,061,499 - BBR_RS19115 0.57 +0.2 2,061,562 - BBR_RS19115 0.89 -1.7 2,061,569 - +0.3 2,061,569 - -1.0 2,061,575 + -0.5 2,061,584 + -1.4 2,061,584 + -2.3 2,061,592 - +0.5 2,061,593 - +0.0 2,061,595 - +0.3 2,061,595 - -0.8 2,061,595 - +1.3 2,061,618 - -1.4 2,061,669 - -1.2 2,061,676 - +1.4 2,061,676 - -0.3 2,061,722 - +0.7 2,061,735 + +3.0 2,061,799 + +3.0 2,061,799 + +1.4 2,061,807 - -1.4 2,061,811 + +0.0 2,061,811 + -0.6 2,061,811 + -2.5 2,061,825 + +2.0 2,061,833 - +3.0 2,061,859 + +2.0 2,061,859 + +1.4 2,061,867 + +1.0 2,061,867 - -0.9 2,061,867 - -1.2 2,061,867 - -1.5 2,061,867 - -1.1 2,061,867 - -0.7 2,061,867 - -1.4 2,061,882 + -0.4 2,061,894 + +0.0 2,061,937 + +3.0 2,061,963 + BBR_RS19120 0.10 +1.4 2,061,963 + BBR_RS19120 0.10 +2.0 2,061,966 - BBR_RS19120 0.10 -1.6 2,061,966 - BBR_RS19120 0.10 -1.8 2,061,968 + BBR_RS19120 0.11 +1.0 2,061,971 - BBR_RS19120 0.11 +6.3 2,061,981 - BBR_RS19120 0.11 +3.0 2,062,028 - BBR_RS19120 0.12 +3.0 2,062,028 - BBR_RS19120 0.12 +2.0 2,062,099 - BBR_RS19120 0.14 +3.0 2,062,128 - BBR_RS19120 0.15 +0.2 2,062,282 - BBR_RS19120 0.19 +0.7 2,062,309 - BBR_RS19120 0.20 +3.0 2,062,313 - BBR_RS19120 0.20 +1.4 2,062,374 + BBR_RS19120 0.22 +3.0 2,062,374 + BBR_RS19120 0.22 +1.0 2,062,382 - BBR_RS19120 0.22 +1.0 2,062,397 - BBR_RS19120 0.22 +2.0 2,062,398 - BBR_RS19120 0.22 +2.0 2,062,422 + BBR_RS19120 0.23 +2.0 2,062,430 - BBR_RS19120 0.23 -0.4 2,062,446 + BBR_RS19120 0.24 +2.0 2,062,454 + BBR_RS19120 0.24 +3.0 2,062,454 - BBR_RS19120 0.24 -0.3 2,062,482 + BBR_RS19120 0.25 -0.2 2,062,499 + BBR_RS19120 0.25 +3.0 2,062,512 - BBR_RS19120 0.26 +2.7 2,062,517 - BBR_RS19120 0.26 +1.0 2,062,566 - BBR_RS19120 0.27 -1.3
Or see this region's nucleotide sequence