Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS18745 and BBR_RS18750 are separated by 54 nucleotides BBR_RS18750 and BBR_RS18755 are separated by 92 nucleotides BBR_RS18755 and BBR_RS18760 are separated by 270 nucleotides
BBR_RS18745: BBR_RS18745 - bifunctional riboflavin kinase/FMN adenylyltransferase, at 1,996,330 to 1,997,454
_RS18745
BBR_RS18750: BBR_RS18750 - pseudouridine synthase, at 1,997,509 to 1,998,663
_RS18750
BBR_RS18755: BBR_RS18755 - 30S ribosome-binding factor RbfA, at 1,998,756 to 1,999,241
_RS18755
BBR_RS18760: BBR_RS18760 - translation initiation factor IF-2, at 1,999,512 to 2,002,334
_RS18760
Position (kb)
1997
1998
1999 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1996.849 kb on - strand, within BBR_RS18745 at 1996.870 kb on - strand, within BBR_RS18745 at 1997.528 kb on - strand at 1997.542 kb on - strand at 1997.542 kb on - strand at 1997.542 kb on - strand at 1997.585 kb on - strand at 1997.585 kb on - strand at 1997.585 kb on - strand at 1997.657 kb on - strand, within BBR_RS18750 at 1997.706 kb on + strand, within BBR_RS18750 at 1997.714 kb on - strand, within BBR_RS18750 at 1997.722 kb on - strand, within BBR_RS18750 at 1997.763 kb on + strand, within BBR_RS18750 at 1997.763 kb on - strand, within BBR_RS18750 at 1997.771 kb on - strand, within BBR_RS18750 at 1997.785 kb on + strand, within BBR_RS18750 at 1997.785 kb on - strand, within BBR_RS18750 at 1997.793 kb on - strand, within BBR_RS18750 at 1997.793 kb on - strand, within BBR_RS18750 at 1997.793 kb on - strand, within BBR_RS18750 at 1997.837 kb on + strand, within BBR_RS18750 at 1997.845 kb on - strand, within BBR_RS18750 at 1997.850 kb on + strand, within BBR_RS18750 at 1997.850 kb on + strand, within BBR_RS18750 at 1997.852 kb on + strand, within BBR_RS18750 at 1997.857 kb on + strand, within BBR_RS18750 at 1997.857 kb on + strand, within BBR_RS18750 at 1997.858 kb on - strand, within BBR_RS18750 at 1997.882 kb on + strand, within BBR_RS18750 at 1997.882 kb on + strand, within BBR_RS18750 at 1997.882 kb on + strand, within BBR_RS18750 at 1997.890 kb on - strand, within BBR_RS18750 at 1997.894 kb on - strand, within BBR_RS18750 at 1997.923 kb on + strand, within BBR_RS18750 at 1997.928 kb on + strand, within BBR_RS18750 at 1997.928 kb on - strand, within BBR_RS18750 at 1997.938 kb on + strand, within BBR_RS18750 at 1997.955 kb on - strand, within BBR_RS18750 at 1997.955 kb on - strand, within BBR_RS18750 at 1997.971 kb on - strand, within BBR_RS18750 at 1998.001 kb on + strand, within BBR_RS18750 at 1998.025 kb on + strand, within BBR_RS18750 at 1998.038 kb on + strand, within BBR_RS18750 at 1998.038 kb on + strand, within BBR_RS18750 at 1998.077 kb on + strand, within BBR_RS18750 at 1998.085 kb on - strand, within BBR_RS18750 at 1998.085 kb on - strand, within BBR_RS18750 at 1998.107 kb on - strand, within BBR_RS18750 at 1998.152 kb on + strand, within BBR_RS18750 at 1998.153 kb on + strand, within BBR_RS18750 at 1998.160 kb on + strand, within BBR_RS18750 at 1998.160 kb on - strand, within BBR_RS18750 at 1998.165 kb on + strand, within BBR_RS18750 at 1998.168 kb on - strand, within BBR_RS18750 at 1998.168 kb on - strand, within BBR_RS18750 at 1998.251 kb on - strand, within BBR_RS18750 at 1998.266 kb on + strand, within BBR_RS18750 at 1998.274 kb on - strand, within BBR_RS18750 at 1998.286 kb on - strand, within BBR_RS18750 at 1998.299 kb on - strand, within BBR_RS18750 at 1998.343 kb on - strand, within BBR_RS18750 at 1998.343 kb on - strand, within BBR_RS18750 at 1998.343 kb on - strand, within BBR_RS18750 at 1998.365 kb on - strand, within BBR_RS18750 at 1998.365 kb on - strand, within BBR_RS18750 at 1998.384 kb on - strand, within BBR_RS18750 at 1998.422 kb on - strand, within BBR_RS18750 at 1998.432 kb on - strand, within BBR_RS18750 at 1998.432 kb on - strand, within BBR_RS18750 at 1998.437 kb on - strand, within BBR_RS18750 at 1998.457 kb on - strand, within BBR_RS18750 at 1998.463 kb on - strand, within BBR_RS18750 at 1998.468 kb on - strand, within BBR_RS18750 at 1998.476 kb on + strand, within BBR_RS18750 at 1998.488 kb on + strand, within BBR_RS18750 at 1998.519 kb on - strand, within BBR_RS18750 at 1998.542 kb on - strand, within BBR_RS18750 at 1998.629 kb on + strand at 1998.651 kb on - strand at 1998.661 kb on + strand at 1998.671 kb on + strand at 1998.706 kb on - strand at 1998.714 kb on + strand at 1998.715 kb on + strand at 1998.722 kb on - strand at 1998.722 kb on - strand at 1998.722 kb on - strand at 1998.723 kb on - strand at 1998.723 kb on - strand at 1998.780 kb on - strand at 1998.780 kb on - strand at 1998.782 kb on - strand at 1998.784 kb on + strand at 1998.825 kb on - strand, within BBR_RS18755 at 1998.826 kb on - strand, within BBR_RS18755 at 1998.846 kb on - strand, within BBR_RS18755 at 1999.301 kb on - strand at 1999.308 kb on - strand at 1999.316 kb on - strand at 1999.321 kb on - strand at 1999.321 kb on - strand at 1999.321 kb on - strand at 1999.323 kb on - strand at 1999.335 kb on + strand at 1999.336 kb on - strand at 1999.343 kb on - strand at 1999.343 kb on - strand at 1999.343 kb on - strand at 1999.345 kb on - strand at 1999.455 kb on + strand at 1999.467 kb on - strand at 1999.471 kb on - strand at 1999.498 kb on - strand at 1999.504 kb on - strand at 1999.518 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 1,996,849 - BBR_RS18745 0.46 -0.0 1,996,870 - BBR_RS18745 0.48 -2.0 1,997,528 - -0.4 1,997,542 - -1.0 1,997,542 - -0.5 1,997,542 - -0.4 1,997,585 - -0.3 1,997,585 - +0.5 1,997,585 - +0.4 1,997,657 - BBR_RS18750 0.13 +0.3 1,997,706 + BBR_RS18750 0.17 -0.6 1,997,714 - BBR_RS18750 0.18 -0.4 1,997,722 - BBR_RS18750 0.18 -0.5 1,997,763 + BBR_RS18750 0.22 -0.8 1,997,763 - BBR_RS18750 0.22 -1.6 1,997,771 - BBR_RS18750 0.23 -1.2 1,997,785 + BBR_RS18750 0.24 -0.5 1,997,785 - BBR_RS18750 0.24 -0.8 1,997,793 - BBR_RS18750 0.25 +0.8 1,997,793 - BBR_RS18750 0.25 -0.4 1,997,793 - BBR_RS18750 0.25 -2.1 1,997,837 + BBR_RS18750 0.28 -1.1 1,997,845 - BBR_RS18750 0.29 -2.3 1,997,850 + BBR_RS18750 0.30 -1.4 1,997,850 + BBR_RS18750 0.30 -1.9 1,997,852 + BBR_RS18750 0.30 -0.4 1,997,857 + BBR_RS18750 0.30 +0.7 1,997,857 + BBR_RS18750 0.30 -0.3 1,997,858 - BBR_RS18750 0.30 +0.7 1,997,882 + BBR_RS18750 0.32 +0.6 1,997,882 + BBR_RS18750 0.32 +0.3 1,997,882 + BBR_RS18750 0.32 +1.1 1,997,890 - BBR_RS18750 0.33 -0.6 1,997,894 - BBR_RS18750 0.33 -1.7 1,997,923 + BBR_RS18750 0.36 -0.7 1,997,928 + BBR_RS18750 0.36 -0.0 1,997,928 - BBR_RS18750 0.36 -0.8 1,997,938 + BBR_RS18750 0.37 -1.5 1,997,955 - BBR_RS18750 0.39 -0.1 1,997,955 - BBR_RS18750 0.39 -1.5 1,997,971 - BBR_RS18750 0.40 -1.0 1,998,001 + BBR_RS18750 0.43 -0.6 1,998,025 + BBR_RS18750 0.45 -0.2 1,998,038 + BBR_RS18750 0.46 +0.1 1,998,038 + BBR_RS18750 0.46 -0.0 1,998,077 + BBR_RS18750 0.49 +1.2 1,998,085 - BBR_RS18750 0.50 -0.7 1,998,085 - BBR_RS18750 0.50 -0.7 1,998,107 - BBR_RS18750 0.52 +0.6 1,998,152 + BBR_RS18750 0.56 +0.4 1,998,153 + BBR_RS18750 0.56 -0.9 1,998,160 + BBR_RS18750 0.56 -0.3 1,998,160 - BBR_RS18750 0.56 -0.7 1,998,165 + BBR_RS18750 0.57 +0.1 1,998,168 - BBR_RS18750 0.57 -1.3 1,998,168 - BBR_RS18750 0.57 +0.7 1,998,251 - BBR_RS18750 0.64 +0.1 1,998,266 + BBR_RS18750 0.66 -1.3 1,998,274 - BBR_RS18750 0.66 -1.0 1,998,286 - BBR_RS18750 0.67 -2.0 1,998,299 - BBR_RS18750 0.68 -1.2 1,998,343 - BBR_RS18750 0.72 +0.0 1,998,343 - BBR_RS18750 0.72 +0.7 1,998,343 - BBR_RS18750 0.72 -0.6 1,998,365 - BBR_RS18750 0.74 -0.8 1,998,365 - BBR_RS18750 0.74 -2.7 1,998,384 - BBR_RS18750 0.76 -2.1 1,998,422 - BBR_RS18750 0.79 +0.4 1,998,432 - BBR_RS18750 0.80 -3.5 1,998,432 - BBR_RS18750 0.80 -0.2 1,998,437 - BBR_RS18750 0.80 +1.2 1,998,457 - BBR_RS18750 0.82 +1.0 1,998,463 - BBR_RS18750 0.83 -0.7 1,998,468 - BBR_RS18750 0.83 -1.9 1,998,476 + BBR_RS18750 0.84 -0.8 1,998,488 + BBR_RS18750 0.85 -1.3 1,998,519 - BBR_RS18750 0.87 +0.1 1,998,542 - BBR_RS18750 0.89 -1.6 1,998,629 + -1.1 1,998,651 - -1.4 1,998,661 + -0.3 1,998,671 + -0.4 1,998,706 - -0.1 1,998,714 + -0.3 1,998,715 + -0.9 1,998,722 - +0.9 1,998,722 - -0.3 1,998,722 - -0.2 1,998,723 - +0.1 1,998,723 - -0.6 1,998,780 - +0.8 1,998,780 - -0.3 1,998,782 - +0.7 1,998,784 + -1.1 1,998,825 - BBR_RS18755 0.14 +1.4 1,998,826 - BBR_RS18755 0.14 +1.0 1,998,846 - BBR_RS18755 0.19 +0.0 1,999,301 - -0.0 1,999,308 - -0.1 1,999,316 - -0.1 1,999,321 - +0.7 1,999,321 - +1.0 1,999,321 - -0.8 1,999,323 - +0.5 1,999,335 + +1.0 1,999,336 - +0.3 1,999,343 - -0.5 1,999,343 - -1.0 1,999,343 - -1.6 1,999,345 - +0.1 1,999,455 + +2.0 1,999,467 - -2.0 1,999,471 - +0.4 1,999,498 - -0.2 1,999,504 - -1.9 1,999,518 - -0.7
Or see this region's nucleotide sequence