Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS17510 and BBR_RS17515 overlap by 4 nucleotides BBR_RS17515 and BBR_RS17520 are separated by 126 nucleotides BBR_RS17520 and BBR_RS17525 overlap by 4 nucleotides
BBR_RS17510: BBR_RS17510 - VWA domain-containing protein, at 1,706,966 to 1,708,003
_RS17510
BBR_RS17515: BBR_RS17515 - hypothetical protein, at 1,708,000 to 1,708,572
_RS17515
BBR_RS17520: BBR_RS17520 - hypothetical protein, at 1,708,699 to 1,709,007
_RS17520
BBR_RS17525: BBR_RS17525 - hypothetical protein, at 1,709,004 to 1,710,584
_RS17525
Position (kb)
1707
1708
1709 Strain fitness (log2 ratio)
-2
-1
0
1 at 1707.002 kb on + strand at 1707.038 kb on - strand at 1707.163 kb on - strand, within BBR_RS17510 at 1707.183 kb on + strand, within BBR_RS17510 at 1707.201 kb on + strand, within BBR_RS17510 at 1707.206 kb on + strand, within BBR_RS17510 at 1707.209 kb on - strand, within BBR_RS17510 at 1707.236 kb on - strand, within BBR_RS17510 at 1707.243 kb on + strand, within BBR_RS17510 at 1707.269 kb on + strand, within BBR_RS17510 at 1707.298 kb on + strand, within BBR_RS17510 at 1707.365 kb on + strand, within BBR_RS17510 at 1707.365 kb on + strand, within BBR_RS17510 at 1707.383 kb on - strand, within BBR_RS17510 at 1707.445 kb on - strand, within BBR_RS17510 at 1707.445 kb on - strand, within BBR_RS17510 at 1707.446 kb on + strand, within BBR_RS17510 at 1707.447 kb on + strand, within BBR_RS17510 at 1707.476 kb on - strand, within BBR_RS17510 at 1707.537 kb on + strand, within BBR_RS17510 at 1707.547 kb on + strand, within BBR_RS17510 at 1707.581 kb on - strand, within BBR_RS17510 at 1707.619 kb on + strand, within BBR_RS17510 at 1707.708 kb on + strand, within BBR_RS17510 at 1707.718 kb on + strand, within BBR_RS17510 at 1707.774 kb on + strand, within BBR_RS17510 at 1707.784 kb on - strand, within BBR_RS17510 at 1707.784 kb on - strand, within BBR_RS17510 at 1707.804 kb on + strand, within BBR_RS17510 at 1707.815 kb on + strand, within BBR_RS17510 at 1707.882 kb on - strand, within BBR_RS17510 at 1707.907 kb on + strand at 1707.912 kb on + strand at 1707.914 kb on + strand at 1707.917 kb on - strand at 1707.919 kb on + strand at 1707.919 kb on + strand at 1707.919 kb on + strand at 1707.920 kb on - strand at 1707.927 kb on - strand at 1707.997 kb on + strand at 1708.007 kb on + strand at 1708.007 kb on + strand at 1708.017 kb on - strand at 1708.017 kb on - strand at 1708.103 kb on + strand, within BBR_RS17515 at 1708.103 kb on + strand, within BBR_RS17515 at 1708.103 kb on + strand, within BBR_RS17515 at 1708.103 kb on + strand, within BBR_RS17515 at 1708.111 kb on - strand, within BBR_RS17515 at 1708.111 kb on - strand, within BBR_RS17515 at 1708.169 kb on - strand, within BBR_RS17515 at 1708.203 kb on - strand, within BBR_RS17515 at 1708.213 kb on - strand, within BBR_RS17515 at 1708.213 kb on - strand, within BBR_RS17515 at 1708.242 kb on + strand, within BBR_RS17515 at 1708.242 kb on + strand, within BBR_RS17515 at 1708.242 kb on + strand, within BBR_RS17515 at 1708.310 kb on + strand, within BBR_RS17515 at 1708.310 kb on + strand, within BBR_RS17515 at 1708.318 kb on - strand, within BBR_RS17515 at 1708.318 kb on - strand, within BBR_RS17515 at 1708.331 kb on + strand, within BBR_RS17515 at 1708.341 kb on + strand, within BBR_RS17515 at 1708.369 kb on + strand, within BBR_RS17515 at 1708.377 kb on - strand, within BBR_RS17515 at 1708.377 kb on - strand, within BBR_RS17515 at 1708.377 kb on - strand, within BBR_RS17515 at 1708.377 kb on - strand, within BBR_RS17515 at 1708.382 kb on - strand, within BBR_RS17515 at 1708.392 kb on - strand, within BBR_RS17515 at 1708.418 kb on - strand, within BBR_RS17515 at 1708.484 kb on - strand, within BBR_RS17515 at 1708.564 kb on - strand at 1708.564 kb on - strand at 1708.577 kb on - strand at 1708.612 kb on + strand at 1708.612 kb on + strand at 1708.645 kb on - strand at 1708.654 kb on - strand at 1708.660 kb on + strand at 1708.683 kb on - strand at 1708.684 kb on - strand at 1708.691 kb on - strand at 1708.708 kb on + strand at 1708.718 kb on + strand at 1708.727 kb on + strand at 1708.800 kb on - strand, within BBR_RS17520 at 1708.810 kb on - strand, within BBR_RS17520 at 1708.810 kb on - strand, within BBR_RS17520 at 1708.827 kb on + strand, within BBR_RS17520 at 1708.827 kb on + strand, within BBR_RS17520 at 1708.836 kb on - strand, within BBR_RS17520 at 1708.883 kb on + strand, within BBR_RS17520 at 1708.893 kb on + strand, within BBR_RS17520 at 1708.962 kb on - strand, within BBR_RS17520 at 1708.963 kb on + strand, within BBR_RS17520 at 1708.975 kb on - strand, within BBR_RS17520 at 1708.998 kb on - strand at 1709.069 kb on + strand at 1709.076 kb on + strand at 1709.145 kb on - strand at 1709.190 kb on + strand, within BBR_RS17525 at 1709.190 kb on + strand, within BBR_RS17525 at 1709.206 kb on - strand, within BBR_RS17525 at 1709.224 kb on - strand, within BBR_RS17525 at 1709.231 kb on + strand, within BBR_RS17525 at 1709.269 kb on - strand, within BBR_RS17525 at 1709.278 kb on + strand, within BBR_RS17525 at 1709.286 kb on - strand, within BBR_RS17525 at 1709.403 kb on + strand, within BBR_RS17525 at 1709.411 kb on + strand, within BBR_RS17525 at 1709.419 kb on - strand, within BBR_RS17525 at 1709.423 kb on + strand, within BBR_RS17525 at 1709.450 kb on - strand, within BBR_RS17525 at 1709.512 kb on - strand, within BBR_RS17525 at 1709.522 kb on - strand, within BBR_RS17525 at 1709.530 kb on - strand, within BBR_RS17525
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 1,707,002 + -1.0 1,707,038 - +0.9 1,707,163 - BBR_RS17510 0.19 +0.9 1,707,183 + BBR_RS17510 0.21 +0.8 1,707,201 + BBR_RS17510 0.23 -0.9 1,707,206 + BBR_RS17510 0.23 -0.6 1,707,209 - BBR_RS17510 0.23 +0.0 1,707,236 - BBR_RS17510 0.26 +0.0 1,707,243 + BBR_RS17510 0.27 -0.1 1,707,269 + BBR_RS17510 0.29 -1.3 1,707,298 + BBR_RS17510 0.32 -2.3 1,707,365 + BBR_RS17510 0.38 -2.0 1,707,365 + BBR_RS17510 0.38 -0.0 1,707,383 - BBR_RS17510 0.40 +0.1 1,707,445 - BBR_RS17510 0.46 -0.0 1,707,445 - BBR_RS17510 0.46 -1.9 1,707,446 + BBR_RS17510 0.46 -0.7 1,707,447 + BBR_RS17510 0.46 +0.8 1,707,476 - BBR_RS17510 0.49 +1.5 1,707,537 + BBR_RS17510 0.55 -0.5 1,707,547 + BBR_RS17510 0.56 -1.0 1,707,581 - BBR_RS17510 0.59 +0.1 1,707,619 + BBR_RS17510 0.63 -1.6 1,707,708 + BBR_RS17510 0.71 +0.2 1,707,718 + BBR_RS17510 0.72 -1.2 1,707,774 + BBR_RS17510 0.78 -0.4 1,707,784 - BBR_RS17510 0.79 -1.2 1,707,784 - BBR_RS17510 0.79 -0.7 1,707,804 + BBR_RS17510 0.81 -1.1 1,707,815 + BBR_RS17510 0.82 -0.7 1,707,882 - BBR_RS17510 0.88 -0.8 1,707,907 + +0.8 1,707,912 + -1.3 1,707,914 + -2.7 1,707,917 - -0.9 1,707,919 + +0.0 1,707,919 + -0.8 1,707,919 + -0.7 1,707,920 - +0.2 1,707,927 - -0.5 1,707,997 + +0.3 1,708,007 + -0.3 1,708,007 + -0.2 1,708,017 - -0.2 1,708,017 - +0.7 1,708,103 + BBR_RS17515 0.18 -1.5 1,708,103 + BBR_RS17515 0.18 +0.0 1,708,103 + BBR_RS17515 0.18 -2.1 1,708,103 + BBR_RS17515 0.18 -0.0 1,708,111 - BBR_RS17515 0.19 -1.5 1,708,111 - BBR_RS17515 0.19 -0.0 1,708,169 - BBR_RS17515 0.29 +0.8 1,708,203 - BBR_RS17515 0.35 -0.2 1,708,213 - BBR_RS17515 0.37 +0.5 1,708,213 - BBR_RS17515 0.37 -0.1 1,708,242 + BBR_RS17515 0.42 -1.3 1,708,242 + BBR_RS17515 0.42 +0.8 1,708,242 + BBR_RS17515 0.42 -1.2 1,708,310 + BBR_RS17515 0.54 -0.9 1,708,310 + BBR_RS17515 0.54 -0.9 1,708,318 - BBR_RS17515 0.55 +0.6 1,708,318 - BBR_RS17515 0.55 -1.5 1,708,331 + BBR_RS17515 0.58 +0.4 1,708,341 + BBR_RS17515 0.60 -1.3 1,708,369 + BBR_RS17515 0.64 -1.0 1,708,377 - BBR_RS17515 0.66 -1.5 1,708,377 - BBR_RS17515 0.66 -1.9 1,708,377 - BBR_RS17515 0.66 -0.7 1,708,377 - BBR_RS17515 0.66 -0.8 1,708,382 - BBR_RS17515 0.67 -2.0 1,708,392 - BBR_RS17515 0.68 +0.0 1,708,418 - BBR_RS17515 0.73 -0.8 1,708,484 - BBR_RS17515 0.84 -0.7 1,708,564 - +0.8 1,708,564 - -0.0 1,708,577 - +0.1 1,708,612 + +0.7 1,708,612 + -0.8 1,708,645 - +0.3 1,708,654 - -0.9 1,708,660 + -0.2 1,708,683 - +0.1 1,708,684 - +0.1 1,708,691 - -0.4 1,708,708 + -0.1 1,708,718 + +0.4 1,708,727 + -0.6 1,708,800 - BBR_RS17520 0.33 +0.0 1,708,810 - BBR_RS17520 0.36 +0.9 1,708,810 - BBR_RS17520 0.36 -0.6 1,708,827 + BBR_RS17520 0.41 +0.7 1,708,827 + BBR_RS17520 0.41 -0.0 1,708,836 - BBR_RS17520 0.44 +0.2 1,708,883 + BBR_RS17520 0.60 -0.2 1,708,893 + BBR_RS17520 0.63 -0.2 1,708,962 - BBR_RS17520 0.85 -1.4 1,708,963 + BBR_RS17520 0.85 +0.7 1,708,975 - BBR_RS17520 0.89 +0.7 1,708,998 - +0.1 1,709,069 + +1.4 1,709,076 + +0.2 1,709,145 - -0.2 1,709,190 + BBR_RS17525 0.12 +0.4 1,709,190 + BBR_RS17525 0.12 -0.2 1,709,206 - BBR_RS17525 0.13 +0.3 1,709,224 - BBR_RS17525 0.14 +1.4 1,709,231 + BBR_RS17525 0.14 -2.4 1,709,269 - BBR_RS17525 0.17 -0.3 1,709,278 + BBR_RS17525 0.17 +0.4 1,709,286 - BBR_RS17525 0.18 +0.6 1,709,403 + BBR_RS17525 0.25 +0.7 1,709,411 + BBR_RS17525 0.26 +0.2 1,709,419 - BBR_RS17525 0.26 -0.9 1,709,423 + BBR_RS17525 0.27 +0.3 1,709,450 - BBR_RS17525 0.28 -1.1 1,709,512 - BBR_RS17525 0.32 +0.1 1,709,522 - BBR_RS17525 0.33 +0.5 1,709,530 - BBR_RS17525 0.33 +0.2
Or see this region's nucleotide sequence