Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS17435 and BBR_RS17440 are separated by 151 nucleotides BBR_RS17440 and BBR_RS17445 are separated by 8 nucleotides BBR_RS17445 and BBR_RS17450 are separated by 93 nucleotides
BBR_RS17435: BBR_RS17435 - universal stress protein, at 1,692,758 to 1,693,723
_RS17435
BBR_RS17440: BBR_RS17440 - DUF3027 domain-containing protein, at 1,693,875 to 1,694,924
_RS17440
BBR_RS17445: BBR_RS17445 - cold shock domain-containing protein, at 1,694,933 to 1,695,322
_RS17445
BBR_RS17450: BBR_RS17450 - sensor histidine kinase, at 1,695,416 to 1,697,341
_RS17450
Position (kb)
1693
1694
1695 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1692.901 kb on + strand, within BBR_RS17435 at 1692.924 kb on + strand, within BBR_RS17435 at 1692.934 kb on - strand, within BBR_RS17435 at 1692.944 kb on - strand, within BBR_RS17435 at 1692.945 kb on - strand, within BBR_RS17435 at 1692.974 kb on - strand, within BBR_RS17435 at 1692.978 kb on + strand, within BBR_RS17435 at 1693.069 kb on - strand, within BBR_RS17435 at 1693.109 kb on + strand, within BBR_RS17435 at 1693.116 kb on + strand, within BBR_RS17435 at 1693.180 kb on + strand, within BBR_RS17435 at 1693.180 kb on + strand, within BBR_RS17435 at 1693.263 kb on - strand, within BBR_RS17435 at 1693.279 kb on + strand, within BBR_RS17435 at 1693.279 kb on + strand, within BBR_RS17435 at 1693.287 kb on - strand, within BBR_RS17435 at 1693.287 kb on - strand, within BBR_RS17435 at 1693.287 kb on - strand, within BBR_RS17435 at 1693.316 kb on - strand, within BBR_RS17435 at 1693.321 kb on - strand, within BBR_RS17435 at 1693.330 kb on - strand, within BBR_RS17435 at 1693.374 kb on + strand, within BBR_RS17435 at 1693.382 kb on - strand, within BBR_RS17435 at 1693.384 kb on + strand, within BBR_RS17435 at 1693.387 kb on - strand, within BBR_RS17435 at 1693.387 kb on - strand, within BBR_RS17435 at 1693.421 kb on - strand, within BBR_RS17435 at 1693.439 kb on + strand, within BBR_RS17435 at 1693.439 kb on + strand, within BBR_RS17435 at 1693.476 kb on - strand, within BBR_RS17435 at 1693.504 kb on + strand, within BBR_RS17435 at 1693.531 kb on + strand, within BBR_RS17435 at 1693.576 kb on + strand, within BBR_RS17435 at 1693.582 kb on - strand, within BBR_RS17435 at 1693.611 kb on - strand, within BBR_RS17435 at 1693.619 kb on - strand, within BBR_RS17435 at 1693.629 kb on + strand at 1693.637 kb on - strand at 1693.712 kb on + strand at 1693.712 kb on + strand at 1693.720 kb on - strand at 1693.738 kb on - strand at 1693.784 kb on - strand at 1693.786 kb on + strand at 1693.852 kb on - strand at 1693.857 kb on - strand at 1693.903 kb on + strand at 1693.957 kb on - strand at 1693.964 kb on + strand at 1693.964 kb on + strand at 1693.972 kb on + strand at 1694.080 kb on - strand, within BBR_RS17440 at 1694.155 kb on - strand, within BBR_RS17440 at 1694.862 kb on - strand at 1694.939 kb on - strand at 1695.371 kb on + strand at 1695.371 kb on + strand at 1695.376 kb on + strand at 1695.383 kb on + strand at 1695.383 kb on + strand at 1695.392 kb on - strand at 1695.394 kb on + strand at 1695.394 kb on + strand at 1695.396 kb on + strand at 1695.450 kb on - strand at 1695.515 kb on + strand at 1695.523 kb on - strand at 1695.523 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 1,692,901 + BBR_RS17435 0.15 -1.1 1,692,924 + BBR_RS17435 0.17 +0.7 1,692,934 - BBR_RS17435 0.18 -0.6 1,692,944 - BBR_RS17435 0.19 -1.5 1,692,945 - BBR_RS17435 0.19 +0.8 1,692,974 - BBR_RS17435 0.22 +0.6 1,692,978 + BBR_RS17435 0.23 +0.3 1,693,069 - BBR_RS17435 0.32 -0.6 1,693,109 + BBR_RS17435 0.36 -1.3 1,693,116 + BBR_RS17435 0.37 +1.5 1,693,180 + BBR_RS17435 0.44 -0.8 1,693,180 + BBR_RS17435 0.44 +0.2 1,693,263 - BBR_RS17435 0.52 +1.8 1,693,279 + BBR_RS17435 0.54 +0.3 1,693,279 + BBR_RS17435 0.54 +0.4 1,693,287 - BBR_RS17435 0.55 -2.2 1,693,287 - BBR_RS17435 0.55 -0.9 1,693,287 - BBR_RS17435 0.55 -2.1 1,693,316 - BBR_RS17435 0.58 -0.4 1,693,321 - BBR_RS17435 0.58 -0.2 1,693,330 - BBR_RS17435 0.59 -0.5 1,693,374 + BBR_RS17435 0.64 -0.7 1,693,382 - BBR_RS17435 0.65 +0.6 1,693,384 + BBR_RS17435 0.65 -0.0 1,693,387 - BBR_RS17435 0.65 +0.7 1,693,387 - BBR_RS17435 0.65 -0.8 1,693,421 - BBR_RS17435 0.69 +1.7 1,693,439 + BBR_RS17435 0.70 +0.1 1,693,439 + BBR_RS17435 0.70 +1.5 1,693,476 - BBR_RS17435 0.74 -0.6 1,693,504 + BBR_RS17435 0.77 +1.6 1,693,531 + BBR_RS17435 0.80 -0.4 1,693,576 + BBR_RS17435 0.85 -0.7 1,693,582 - BBR_RS17435 0.85 +0.3 1,693,611 - BBR_RS17435 0.88 -1.6 1,693,619 - BBR_RS17435 0.89 -1.1 1,693,629 + +0.6 1,693,637 - -1.5 1,693,712 + -0.4 1,693,712 + +0.5 1,693,720 - -0.4 1,693,738 - +0.9 1,693,784 - -1.0 1,693,786 + -0.1 1,693,852 - -0.3 1,693,857 - +1.5 1,693,903 + -0.7 1,693,957 - -0.3 1,693,964 + +0.5 1,693,964 + +1.0 1,693,972 + +0.7 1,694,080 - BBR_RS17440 0.20 -2.4 1,694,155 - BBR_RS17440 0.27 -0.8 1,694,862 - -2.4 1,694,939 - -1.3 1,695,371 + +0.9 1,695,371 + -1.6 1,695,376 + -0.5 1,695,383 + -0.6 1,695,383 + +0.8 1,695,392 - +0.8 1,695,394 + +1.0 1,695,394 + +0.7 1,695,396 + +0.0 1,695,450 - +1.4 1,695,515 + +0.8 1,695,523 - +0.2 1,695,523 - +0.6
Or see this region's nucleotide sequence