Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS17365 and BBR_RS17370 overlap by 1 nucleotides BBR_RS17370 and BBR_RS17375 are separated by 18 nucleotides BBR_RS17375 and BBR_RS17380 are separated by 52 nucleotides BBR_RS17380 and BBR_RS17385 are separated by 203 nucleotides
BBR_RS17365: BBR_RS17365 - ABC transporter permease, at 1,674,452 to 1,675,129
_RS17365
BBR_RS17370: BBR_RS17370 - ABC transporter substrate-binding protein, at 1,675,129 to 1,675,968
_RS17370
BBR_RS17375: BBR_RS17375 - amino acid ABC transporter ATP-binding protein, at 1,675,987 to 1,676,829
_RS17375
BBR_RS17380: BBR_RS17380 - hypothetical protein, at 1,676,882 to 1,677,433
_RS17380
BBR_RS17385: BBR_RS17385 - aspartate--tRNA ligase, at 1,677,637 to 1,679,436
_RS17385
Position (kb)
1675
1676
1677 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1675.018 kb on + strand, within BBR_RS17365 at 1675.035 kb on + strand, within BBR_RS17365 at 1675.040 kb on + strand, within BBR_RS17365 at 1675.050 kb on + strand, within BBR_RS17365 at 1675.063 kb on + strand at 1675.063 kb on + strand at 1675.073 kb on + strand at 1675.090 kb on - strand at 1675.100 kb on - strand at 1675.109 kb on - strand at 1675.127 kb on + strand at 1675.146 kb on + strand at 1675.146 kb on + strand at 1675.169 kb on - strand at 1675.173 kb on + strand at 1675.178 kb on + strand at 1675.181 kb on - strand at 1675.186 kb on - strand at 1675.201 kb on - strand at 1675.214 kb on + strand, within BBR_RS17370 at 1675.229 kb on - strand, within BBR_RS17370 at 1675.229 kb on - strand, within BBR_RS17370 at 1675.243 kb on + strand, within BBR_RS17370 at 1675.243 kb on + strand, within BBR_RS17370 at 1675.278 kb on - strand, within BBR_RS17370 at 1675.280 kb on + strand, within BBR_RS17370 at 1675.315 kb on - strand, within BBR_RS17370 at 1675.337 kb on + strand, within BBR_RS17370 at 1675.345 kb on - strand, within BBR_RS17370 at 1675.345 kb on - strand, within BBR_RS17370 at 1675.345 kb on - strand, within BBR_RS17370 at 1675.345 kb on - strand, within BBR_RS17370 at 1675.346 kb on - strand, within BBR_RS17370 at 1675.363 kb on + strand, within BBR_RS17370 at 1675.371 kb on - strand, within BBR_RS17370 at 1675.377 kb on + strand, within BBR_RS17370 at 1675.381 kb on - strand, within BBR_RS17370 at 1675.408 kb on - strand, within BBR_RS17370 at 1675.429 kb on + strand, within BBR_RS17370 at 1675.553 kb on - strand, within BBR_RS17370 at 1675.583 kb on + strand, within BBR_RS17370 at 1675.614 kb on - strand, within BBR_RS17370 at 1675.614 kb on - strand, within BBR_RS17370 at 1675.692 kb on - strand, within BBR_RS17370 at 1675.692 kb on - strand, within BBR_RS17370 at 1675.787 kb on - strand, within BBR_RS17370 at 1675.831 kb on - strand, within BBR_RS17370 at 1675.895 kb on + strand at 1675.895 kb on + strand at 1675.898 kb on + strand at 1675.898 kb on + strand at 1675.905 kb on + strand at 1675.906 kb on - strand at 1675.931 kb on + strand at 1675.952 kb on - strand at 1675.988 kb on + strand at 1676.017 kb on - strand at 1676.057 kb on + strand at 1676.065 kb on - strand at 1676.119 kb on - strand, within BBR_RS17375 at 1676.142 kb on + strand, within BBR_RS17375 at 1676.225 kb on - strand, within BBR_RS17375 at 1676.240 kb on + strand, within BBR_RS17375 at 1676.246 kb on + strand, within BBR_RS17375 at 1676.246 kb on + strand, within BBR_RS17375 at 1676.248 kb on - strand, within BBR_RS17375 at 1676.248 kb on - strand, within BBR_RS17375 at 1676.272 kb on + strand, within BBR_RS17375 at 1676.386 kb on - strand, within BBR_RS17375 at 1676.386 kb on - strand, within BBR_RS17375 at 1676.472 kb on - strand, within BBR_RS17375 at 1676.489 kb on - strand, within BBR_RS17375 at 1676.503 kb on + strand, within BBR_RS17375 at 1676.503 kb on + strand, within BBR_RS17375 at 1676.511 kb on - strand, within BBR_RS17375 at 1676.515 kb on + strand, within BBR_RS17375 at 1676.525 kb on + strand, within BBR_RS17375 at 1676.615 kb on + strand, within BBR_RS17375 at 1676.662 kb on + strand, within BBR_RS17375 at 1676.704 kb on - strand, within BBR_RS17375 at 1676.722 kb on + strand, within BBR_RS17375 at 1676.760 kb on - strand at 1676.768 kb on + strand at 1676.853 kb on - strand at 1676.853 kb on - strand at 1676.867 kb on + strand at 1676.892 kb on + strand at 1676.938 kb on + strand, within BBR_RS17380 at 1677.007 kb on + strand, within BBR_RS17380 at 1677.078 kb on + strand, within BBR_RS17380 at 1677.078 kb on + strand, within BBR_RS17380 at 1677.094 kb on - strand, within BBR_RS17380 at 1677.094 kb on - strand, within BBR_RS17380 at 1677.096 kb on - strand, within BBR_RS17380 at 1677.119 kb on - strand, within BBR_RS17380 at 1677.127 kb on + strand, within BBR_RS17380 at 1677.135 kb on - strand, within BBR_RS17380 at 1677.135 kb on - strand, within BBR_RS17380 at 1677.137 kb on + strand, within BBR_RS17380 at 1677.145 kb on - strand, within BBR_RS17380 at 1677.160 kb on - strand, within BBR_RS17380 at 1677.175 kb on - strand, within BBR_RS17380 at 1677.197 kb on + strand, within BBR_RS17380 at 1677.197 kb on + strand, within BBR_RS17380 at 1677.223 kb on - strand, within BBR_RS17380 at 1677.241 kb on - strand, within BBR_RS17380 at 1677.254 kb on - strand, within BBR_RS17380 at 1677.281 kb on + strand, within BBR_RS17380 at 1677.289 kb on - strand, within BBR_RS17380 at 1677.289 kb on - strand, within BBR_RS17380 at 1677.289 kb on - strand, within BBR_RS17380 at 1677.346 kb on + strand, within BBR_RS17380 at 1677.354 kb on - strand, within BBR_RS17380 at 1677.360 kb on + strand, within BBR_RS17380 at 1677.371 kb on - strand, within BBR_RS17380 at 1677.384 kb on + strand at 1677.392 kb on - strand at 1677.392 kb on - strand at 1677.392 kb on - strand at 1677.397 kb on - strand at 1677.489 kb on - strand at 1677.490 kb on + strand at 1677.498 kb on - strand at 1677.498 kb on - strand at 1677.595 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 1,675,018 + BBR_RS17365 0.83 +1.0 1,675,035 + BBR_RS17365 0.86 -1.3 1,675,040 + BBR_RS17365 0.87 -1.8 1,675,050 + BBR_RS17365 0.88 -2.0 1,675,063 + -0.3 1,675,063 + -0.4 1,675,073 + -1.2 1,675,090 - -2.1 1,675,100 - -1.3 1,675,109 - +0.2 1,675,127 + +0.7 1,675,146 + -1.3 1,675,146 + -1.0 1,675,169 - -0.6 1,675,173 + -0.1 1,675,178 + -0.3 1,675,181 - +1.7 1,675,186 - -0.3 1,675,201 - -0.4 1,675,214 + BBR_RS17370 0.10 +1.3 1,675,229 - BBR_RS17370 0.12 -0.0 1,675,229 - BBR_RS17370 0.12 -0.6 1,675,243 + BBR_RS17370 0.14 -1.2 1,675,243 + BBR_RS17370 0.14 -1.0 1,675,278 - BBR_RS17370 0.18 +0.2 1,675,280 + BBR_RS17370 0.18 -0.1 1,675,315 - BBR_RS17370 0.22 -1.0 1,675,337 + BBR_RS17370 0.25 -0.8 1,675,345 - BBR_RS17370 0.26 +0.3 1,675,345 - BBR_RS17370 0.26 -2.3 1,675,345 - BBR_RS17370 0.26 -0.2 1,675,345 - BBR_RS17370 0.26 -3.0 1,675,346 - BBR_RS17370 0.26 +0.0 1,675,363 + BBR_RS17370 0.28 -0.5 1,675,371 - BBR_RS17370 0.29 -1.0 1,675,377 + BBR_RS17370 0.30 -1.2 1,675,381 - BBR_RS17370 0.30 -1.2 1,675,408 - BBR_RS17370 0.33 -1.6 1,675,429 + BBR_RS17370 0.36 +0.3 1,675,553 - BBR_RS17370 0.50 -0.3 1,675,583 + BBR_RS17370 0.54 +1.6 1,675,614 - BBR_RS17370 0.58 -1.1 1,675,614 - BBR_RS17370 0.58 -1.1 1,675,692 - BBR_RS17370 0.67 -1.2 1,675,692 - BBR_RS17370 0.67 -0.8 1,675,787 - BBR_RS17370 0.78 -0.4 1,675,831 - BBR_RS17370 0.84 -1.6 1,675,895 + -1.3 1,675,895 + -0.8 1,675,898 + -1.1 1,675,898 + -0.1 1,675,905 + -2.1 1,675,906 - -1.7 1,675,931 + -0.3 1,675,952 - +0.0 1,675,988 + -0.9 1,676,017 - -1.5 1,676,057 + -0.0 1,676,065 - -1.6 1,676,119 - BBR_RS17375 0.16 -2.5 1,676,142 + BBR_RS17375 0.18 +0.3 1,676,225 - BBR_RS17375 0.28 -0.8 1,676,240 + BBR_RS17375 0.30 -0.8 1,676,246 + BBR_RS17375 0.31 -0.6 1,676,246 + BBR_RS17375 0.31 -0.9 1,676,248 - BBR_RS17375 0.31 -0.3 1,676,248 - BBR_RS17375 0.31 -0.5 1,676,272 + BBR_RS17375 0.34 -0.7 1,676,386 - BBR_RS17375 0.47 -0.0 1,676,386 - BBR_RS17375 0.47 -0.8 1,676,472 - BBR_RS17375 0.58 -1.0 1,676,489 - BBR_RS17375 0.60 -1.9 1,676,503 + BBR_RS17375 0.61 -0.2 1,676,503 + BBR_RS17375 0.61 -0.1 1,676,511 - BBR_RS17375 0.62 -1.7 1,676,515 + BBR_RS17375 0.63 +1.3 1,676,525 + BBR_RS17375 0.64 -1.4 1,676,615 + BBR_RS17375 0.74 -0.8 1,676,662 + BBR_RS17375 0.80 -2.0 1,676,704 - BBR_RS17375 0.85 -0.9 1,676,722 + BBR_RS17375 0.87 -1.4 1,676,760 - -1.4 1,676,768 + -2.2 1,676,853 - +0.3 1,676,853 - -0.4 1,676,867 + -0.7 1,676,892 + +0.9 1,676,938 + BBR_RS17380 0.10 -0.4 1,677,007 + BBR_RS17380 0.23 +0.3 1,677,078 + BBR_RS17380 0.36 -0.1 1,677,078 + BBR_RS17380 0.36 -0.2 1,677,094 - BBR_RS17380 0.38 +0.1 1,677,094 - BBR_RS17380 0.38 -0.1 1,677,096 - BBR_RS17380 0.39 +0.6 1,677,119 - BBR_RS17380 0.43 +0.4 1,677,127 + BBR_RS17380 0.44 -0.0 1,677,135 - BBR_RS17380 0.46 +1.0 1,677,135 - BBR_RS17380 0.46 +1.3 1,677,137 + BBR_RS17380 0.46 -0.3 1,677,145 - BBR_RS17380 0.48 -0.8 1,677,160 - BBR_RS17380 0.50 -2.3 1,677,175 - BBR_RS17380 0.53 -0.1 1,677,197 + BBR_RS17380 0.57 +0.4 1,677,197 + BBR_RS17380 0.57 -0.4 1,677,223 - BBR_RS17380 0.62 +0.8 1,677,241 - BBR_RS17380 0.65 -0.5 1,677,254 - BBR_RS17380 0.67 +0.0 1,677,281 + BBR_RS17380 0.72 +0.6 1,677,289 - BBR_RS17380 0.74 +1.5 1,677,289 - BBR_RS17380 0.74 -0.3 1,677,289 - BBR_RS17380 0.74 -0.3 1,677,346 + BBR_RS17380 0.84 +2.2 1,677,354 - BBR_RS17380 0.86 +0.5 1,677,360 + BBR_RS17380 0.87 -0.1 1,677,371 - BBR_RS17380 0.89 +0.6 1,677,384 + -0.7 1,677,392 - +0.1 1,677,392 - -1.7 1,677,392 - -0.4 1,677,397 - -0.2 1,677,489 - -0.4 1,677,490 + +0.4 1,677,498 - +0.0 1,677,498 - -0.1 1,677,595 + +0.4
Or see this region's nucleotide sequence