Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS17360 and BBR_RS17365 are separated by 5 nucleotides BBR_RS17365 and BBR_RS17370 overlap by 1 nucleotides BBR_RS17370 and BBR_RS17375 are separated by 18 nucleotides BBR_RS17375 and BBR_RS17380 are separated by 52 nucleotides
BBR_RS17360: BBR_RS17360 - amino acid ABC transporter permease, at 1,673,346 to 1,674,446
_RS17360
BBR_RS17365: BBR_RS17365 - ABC transporter permease, at 1,674,452 to 1,675,129
_RS17365
BBR_RS17370: BBR_RS17370 - ABC transporter substrate-binding protein, at 1,675,129 to 1,675,968
_RS17370
BBR_RS17375: BBR_RS17375 - amino acid ABC transporter ATP-binding protein, at 1,675,987 to 1,676,829
_RS17375
BBR_RS17380: BBR_RS17380 - hypothetical protein, at 1,676,882 to 1,677,433
_RS17380
Position (kb)
1675
1676 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1674.141 kb on - strand, within BBR_RS17360 at 1674.145 kb on + strand, within BBR_RS17360 at 1674.145 kb on + strand, within BBR_RS17360 at 1674.169 kb on - strand, within BBR_RS17360 at 1674.181 kb on + strand, within BBR_RS17360 at 1674.189 kb on - strand, within BBR_RS17360 at 1674.214 kb on - strand, within BBR_RS17360 at 1674.242 kb on - strand, within BBR_RS17360 at 1674.242 kb on - strand, within BBR_RS17360 at 1674.255 kb on + strand, within BBR_RS17360 at 1674.265 kb on - strand, within BBR_RS17360 at 1674.270 kb on + strand, within BBR_RS17360 at 1674.278 kb on + strand, within BBR_RS17360 at 1674.313 kb on - strand, within BBR_RS17360 at 1674.344 kb on - strand at 1674.344 kb on - strand at 1674.360 kb on - strand at 1674.380 kb on + strand at 1674.396 kb on + strand at 1674.396 kb on + strand at 1674.420 kb on - strand at 1674.455 kb on + strand at 1674.455 kb on + strand at 1674.465 kb on - strand at 1674.465 kb on - strand at 1674.509 kb on + strand at 1674.553 kb on - strand, within BBR_RS17365 at 1674.633 kb on + strand, within BBR_RS17365 at 1674.668 kb on - strand, within BBR_RS17365 at 1674.738 kb on - strand, within BBR_RS17365 at 1674.753 kb on + strand, within BBR_RS17365 at 1674.753 kb on + strand, within BBR_RS17365 at 1674.761 kb on - strand, within BBR_RS17365 at 1674.816 kb on + strand, within BBR_RS17365 at 1674.816 kb on + strand, within BBR_RS17365 at 1674.828 kb on - strand, within BBR_RS17365 at 1674.945 kb on + strand, within BBR_RS17365 at 1674.963 kb on - strand, within BBR_RS17365 at 1674.973 kb on - strand, within BBR_RS17365 at 1674.986 kb on - strand, within BBR_RS17365 at 1675.018 kb on + strand, within BBR_RS17365 at 1675.035 kb on + strand, within BBR_RS17365 at 1675.040 kb on + strand, within BBR_RS17365 at 1675.050 kb on + strand, within BBR_RS17365 at 1675.063 kb on + strand at 1675.063 kb on + strand at 1675.073 kb on + strand at 1675.090 kb on - strand at 1675.100 kb on - strand at 1675.109 kb on - strand at 1675.127 kb on + strand at 1675.146 kb on + strand at 1675.146 kb on + strand at 1675.169 kb on - strand at 1675.173 kb on + strand at 1675.178 kb on + strand at 1675.181 kb on - strand at 1675.186 kb on - strand at 1675.201 kb on - strand at 1675.214 kb on + strand, within BBR_RS17370 at 1675.229 kb on - strand, within BBR_RS17370 at 1675.229 kb on - strand, within BBR_RS17370 at 1675.243 kb on + strand, within BBR_RS17370 at 1675.243 kb on + strand, within BBR_RS17370 at 1675.278 kb on - strand, within BBR_RS17370 at 1675.280 kb on + strand, within BBR_RS17370 at 1675.315 kb on - strand, within BBR_RS17370 at 1675.337 kb on + strand, within BBR_RS17370 at 1675.345 kb on - strand, within BBR_RS17370 at 1675.345 kb on - strand, within BBR_RS17370 at 1675.345 kb on - strand, within BBR_RS17370 at 1675.345 kb on - strand, within BBR_RS17370 at 1675.346 kb on - strand, within BBR_RS17370 at 1675.363 kb on + strand, within BBR_RS17370 at 1675.371 kb on - strand, within BBR_RS17370 at 1675.377 kb on + strand, within BBR_RS17370 at 1675.381 kb on - strand, within BBR_RS17370 at 1675.408 kb on - strand, within BBR_RS17370 at 1675.429 kb on + strand, within BBR_RS17370 at 1675.553 kb on - strand, within BBR_RS17370 at 1675.583 kb on + strand, within BBR_RS17370 at 1675.614 kb on - strand, within BBR_RS17370 at 1675.614 kb on - strand, within BBR_RS17370 at 1675.692 kb on - strand, within BBR_RS17370 at 1675.692 kb on - strand, within BBR_RS17370 at 1675.787 kb on - strand, within BBR_RS17370 at 1675.831 kb on - strand, within BBR_RS17370 at 1675.895 kb on + strand at 1675.895 kb on + strand at 1675.898 kb on + strand at 1675.898 kb on + strand at 1675.905 kb on + strand at 1675.906 kb on - strand at 1675.931 kb on + strand at 1675.952 kb on - strand at 1675.988 kb on + strand at 1676.017 kb on - strand at 1676.057 kb on + strand at 1676.065 kb on - strand at 1676.119 kb on - strand, within BBR_RS17375 at 1676.142 kb on + strand, within BBR_RS17375 at 1676.225 kb on - strand, within BBR_RS17375 at 1676.240 kb on + strand, within BBR_RS17375 at 1676.246 kb on + strand, within BBR_RS17375 at 1676.246 kb on + strand, within BBR_RS17375 at 1676.248 kb on - strand, within BBR_RS17375 at 1676.248 kb on - strand, within BBR_RS17375 at 1676.272 kb on + strand, within BBR_RS17375 at 1676.386 kb on - strand, within BBR_RS17375 at 1676.386 kb on - strand, within BBR_RS17375 at 1676.472 kb on - strand, within BBR_RS17375 at 1676.489 kb on - strand, within BBR_RS17375 at 1676.503 kb on + strand, within BBR_RS17375 at 1676.503 kb on + strand, within BBR_RS17375 at 1676.511 kb on - strand, within BBR_RS17375 at 1676.515 kb on + strand, within BBR_RS17375 at 1676.525 kb on + strand, within BBR_RS17375 at 1676.615 kb on + strand, within BBR_RS17375 at 1676.662 kb on + strand, within BBR_RS17375 at 1676.704 kb on - strand, within BBR_RS17375 at 1676.722 kb on + strand, within BBR_RS17375 at 1676.760 kb on - strand at 1676.768 kb on + strand at 1676.853 kb on - strand at 1676.853 kb on - strand at 1676.867 kb on + strand at 1676.892 kb on + strand at 1676.938 kb on + strand, within BBR_RS17380
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 1,674,141 - BBR_RS17360 0.72 +0.4 1,674,145 + BBR_RS17360 0.73 -1.0 1,674,145 + BBR_RS17360 0.73 -0.7 1,674,169 - BBR_RS17360 0.75 +0.2 1,674,181 + BBR_RS17360 0.76 -0.3 1,674,189 - BBR_RS17360 0.77 +0.2 1,674,214 - BBR_RS17360 0.79 -0.2 1,674,242 - BBR_RS17360 0.81 -1.8 1,674,242 - BBR_RS17360 0.81 -0.9 1,674,255 + BBR_RS17360 0.83 -0.3 1,674,265 - BBR_RS17360 0.83 -0.2 1,674,270 + BBR_RS17360 0.84 -0.7 1,674,278 + BBR_RS17360 0.85 -1.9 1,674,313 - BBR_RS17360 0.88 -0.2 1,674,344 - -0.8 1,674,344 - +0.9 1,674,360 - -0.7 1,674,380 + -0.5 1,674,396 + -2.3 1,674,396 + +0.5 1,674,420 - -0.4 1,674,455 + -0.7 1,674,455 + -0.6 1,674,465 - -0.9 1,674,465 - -1.1 1,674,509 + -0.6 1,674,553 - BBR_RS17365 0.15 -1.6 1,674,633 + BBR_RS17365 0.27 -0.7 1,674,668 - BBR_RS17365 0.32 -0.1 1,674,738 - BBR_RS17365 0.42 +0.2 1,674,753 + BBR_RS17365 0.44 +0.1 1,674,753 + BBR_RS17365 0.44 -0.8 1,674,761 - BBR_RS17365 0.46 +1.6 1,674,816 + BBR_RS17365 0.54 -2.5 1,674,816 + BBR_RS17365 0.54 -2.8 1,674,828 - BBR_RS17365 0.55 -1.1 1,674,945 + BBR_RS17365 0.73 +2.2 1,674,963 - BBR_RS17365 0.75 -0.3 1,674,973 - BBR_RS17365 0.77 -0.2 1,674,986 - BBR_RS17365 0.79 -0.2 1,675,018 + BBR_RS17365 0.83 +1.0 1,675,035 + BBR_RS17365 0.86 -1.3 1,675,040 + BBR_RS17365 0.87 -1.8 1,675,050 + BBR_RS17365 0.88 -2.0 1,675,063 + -0.3 1,675,063 + -0.4 1,675,073 + -1.2 1,675,090 - -2.1 1,675,100 - -1.3 1,675,109 - +0.2 1,675,127 + +0.7 1,675,146 + -1.3 1,675,146 + -1.0 1,675,169 - -0.6 1,675,173 + -0.1 1,675,178 + -0.3 1,675,181 - +1.7 1,675,186 - -0.3 1,675,201 - -0.4 1,675,214 + BBR_RS17370 0.10 +1.3 1,675,229 - BBR_RS17370 0.12 -0.0 1,675,229 - BBR_RS17370 0.12 -0.6 1,675,243 + BBR_RS17370 0.14 -1.2 1,675,243 + BBR_RS17370 0.14 -1.0 1,675,278 - BBR_RS17370 0.18 +0.2 1,675,280 + BBR_RS17370 0.18 -0.1 1,675,315 - BBR_RS17370 0.22 -1.0 1,675,337 + BBR_RS17370 0.25 -0.8 1,675,345 - BBR_RS17370 0.26 +0.3 1,675,345 - BBR_RS17370 0.26 -2.3 1,675,345 - BBR_RS17370 0.26 -0.2 1,675,345 - BBR_RS17370 0.26 -3.0 1,675,346 - BBR_RS17370 0.26 +0.0 1,675,363 + BBR_RS17370 0.28 -0.5 1,675,371 - BBR_RS17370 0.29 -1.0 1,675,377 + BBR_RS17370 0.30 -1.2 1,675,381 - BBR_RS17370 0.30 -1.2 1,675,408 - BBR_RS17370 0.33 -1.6 1,675,429 + BBR_RS17370 0.36 +0.3 1,675,553 - BBR_RS17370 0.50 -0.3 1,675,583 + BBR_RS17370 0.54 +1.6 1,675,614 - BBR_RS17370 0.58 -1.1 1,675,614 - BBR_RS17370 0.58 -1.1 1,675,692 - BBR_RS17370 0.67 -1.2 1,675,692 - BBR_RS17370 0.67 -0.8 1,675,787 - BBR_RS17370 0.78 -0.4 1,675,831 - BBR_RS17370 0.84 -1.6 1,675,895 + -1.3 1,675,895 + -0.8 1,675,898 + -1.1 1,675,898 + -0.1 1,675,905 + -2.1 1,675,906 - -1.7 1,675,931 + -0.3 1,675,952 - +0.0 1,675,988 + -0.9 1,676,017 - -1.5 1,676,057 + -0.0 1,676,065 - -1.6 1,676,119 - BBR_RS17375 0.16 -2.5 1,676,142 + BBR_RS17375 0.18 +0.3 1,676,225 - BBR_RS17375 0.28 -0.8 1,676,240 + BBR_RS17375 0.30 -0.8 1,676,246 + BBR_RS17375 0.31 -0.6 1,676,246 + BBR_RS17375 0.31 -0.9 1,676,248 - BBR_RS17375 0.31 -0.3 1,676,248 - BBR_RS17375 0.31 -0.5 1,676,272 + BBR_RS17375 0.34 -0.7 1,676,386 - BBR_RS17375 0.47 -0.0 1,676,386 - BBR_RS17375 0.47 -0.8 1,676,472 - BBR_RS17375 0.58 -1.0 1,676,489 - BBR_RS17375 0.60 -1.9 1,676,503 + BBR_RS17375 0.61 -0.2 1,676,503 + BBR_RS17375 0.61 -0.1 1,676,511 - BBR_RS17375 0.62 -1.7 1,676,515 + BBR_RS17375 0.63 +1.3 1,676,525 + BBR_RS17375 0.64 -1.4 1,676,615 + BBR_RS17375 0.74 -0.8 1,676,662 + BBR_RS17375 0.80 -2.0 1,676,704 - BBR_RS17375 0.85 -0.9 1,676,722 + BBR_RS17375 0.87 -1.4 1,676,760 - -1.4 1,676,768 + -2.2 1,676,853 - +0.3 1,676,853 - -0.4 1,676,867 + -0.7 1,676,892 + +0.9 1,676,938 + BBR_RS17380 0.10 -0.4
Or see this region's nucleotide sequence