Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16740 and BBR_RS16745 are separated by 12 nucleotides BBR_RS16745 and BBR_RS16750 overlap by 1 nucleotides BBR_RS16750 and BBR_RS16755 are separated by 224 nucleotides
BBR_RS16740: BBR_RS16740 - SMC-Scp complex subunit ScpB, at 1,528,503 to 1,529,177
_RS16740
BBR_RS16745: BBR_RS16745 - segregation/condensation protein A, at 1,529,190 to 1,530,041
_RS16745
BBR_RS16750: BBR_RS16750 - ParA family protein, at 1,530,041 to 1,530,880
_RS16750
BBR_RS16755: BBR_RS16755 - site-specific tyrosine recombinase XerD, at 1,531,105 to 1,532,034
_RS16755
Position (kb)
1530
1531 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1529.050 kb on - strand, within BBR_RS16740 at 1529.050 kb on - strand, within BBR_RS16740 at 1529.095 kb on + strand, within BBR_RS16740 at 1529.108 kb on - strand, within BBR_RS16740 at 1529.129 kb on + strand at 1529.129 kb on + strand at 1529.139 kb on - strand at 1529.326 kb on + strand, within BBR_RS16745 at 1529.326 kb on + strand, within BBR_RS16745 at 1529.334 kb on - strand, within BBR_RS16745 at 1529.334 kb on - strand, within BBR_RS16745 at 1529.334 kb on - strand, within BBR_RS16745 at 1529.378 kb on + strand, within BBR_RS16745 at 1529.386 kb on - strand, within BBR_RS16745 at 1529.433 kb on - strand, within BBR_RS16745 at 1529.556 kb on - strand, within BBR_RS16745 at 1529.574 kb on - strand, within BBR_RS16745 at 1529.594 kb on + strand, within BBR_RS16745 at 1529.597 kb on - strand, within BBR_RS16745 at 1529.679 kb on + strand, within BBR_RS16745 at 1529.708 kb on - strand, within BBR_RS16745 at 1529.731 kb on - strand, within BBR_RS16745 at 1529.831 kb on - strand, within BBR_RS16745 at 1529.954 kb on + strand, within BBR_RS16745 at 1529.954 kb on + strand, within BBR_RS16745 at 1529.954 kb on + strand, within BBR_RS16745 at 1529.962 kb on - strand at 1529.962 kb on - strand at 1529.962 kb on - strand at 1529.991 kb on + strand at 1530.010 kb on + strand at 1530.300 kb on + strand, within BBR_RS16750 at 1530.322 kb on + strand, within BBR_RS16750 at 1530.339 kb on - strand, within BBR_RS16750 at 1530.339 kb on - strand, within BBR_RS16750 at 1530.355 kb on + strand, within BBR_RS16750 at 1530.374 kb on + strand, within BBR_RS16750 at 1530.382 kb on - strand, within BBR_RS16750 at 1530.478 kb on - strand, within BBR_RS16750 at 1530.478 kb on - strand, within BBR_RS16750 at 1530.506 kb on - strand, within BBR_RS16750 at 1530.676 kb on - strand, within BBR_RS16750 at 1530.745 kb on + strand, within BBR_RS16750 at 1530.767 kb on + strand, within BBR_RS16750 at 1530.783 kb on + strand, within BBR_RS16750 at 1530.853 kb on - strand at 1530.989 kb on + strand at 1530.989 kb on + strand at 1531.026 kb on + strand at 1531.034 kb on - strand at 1531.039 kb on - strand at 1531.073 kb on - strand at 1531.074 kb on - strand at 1531.074 kb on - strand at 1531.110 kb on - strand at 1531.110 kb on - strand at 1531.166 kb on + strand at 1531.181 kb on + strand at 1531.206 kb on - strand, within BBR_RS16755 at 1531.217 kb on + strand, within BBR_RS16755 at 1531.217 kb on - strand, within BBR_RS16755 at 1531.225 kb on - strand, within BBR_RS16755 at 1531.227 kb on - strand, within BBR_RS16755 at 1531.269 kb on + strand, within BBR_RS16755 at 1531.349 kb on - strand, within BBR_RS16755 at 1531.431 kb on - strand, within BBR_RS16755 at 1531.444 kb on + strand, within BBR_RS16755 at 1531.479 kb on + strand, within BBR_RS16755 at 1531.484 kb on + strand, within BBR_RS16755 at 1531.499 kb on + strand, within BBR_RS16755 at 1531.507 kb on - strand, within BBR_RS16755 at 1531.520 kb on + strand, within BBR_RS16755 at 1531.615 kb on - strand, within BBR_RS16755 at 1531.687 kb on + strand, within BBR_RS16755 at 1531.704 kb on - strand, within BBR_RS16755 at 1531.847 kb on + strand, within BBR_RS16755
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 1,529,050 - BBR_RS16740 0.81 -0.5 1,529,050 - BBR_RS16740 0.81 +0.9 1,529,095 + BBR_RS16740 0.88 +1.7 1,529,108 - BBR_RS16740 0.90 -0.5 1,529,129 + +0.1 1,529,129 + +0.2 1,529,139 - -1.2 1,529,326 + BBR_RS16745 0.16 +0.3 1,529,326 + BBR_RS16745 0.16 -0.3 1,529,334 - BBR_RS16745 0.17 -0.1 1,529,334 - BBR_RS16745 0.17 -0.1 1,529,334 - BBR_RS16745 0.17 -0.9 1,529,378 + BBR_RS16745 0.22 +0.6 1,529,386 - BBR_RS16745 0.23 +0.6 1,529,433 - BBR_RS16745 0.29 +0.7 1,529,556 - BBR_RS16745 0.43 -0.5 1,529,574 - BBR_RS16745 0.45 -0.6 1,529,594 + BBR_RS16745 0.47 +1.0 1,529,597 - BBR_RS16745 0.48 -0.5 1,529,679 + BBR_RS16745 0.57 -1.1 1,529,708 - BBR_RS16745 0.61 -0.6 1,529,731 - BBR_RS16745 0.63 -1.3 1,529,831 - BBR_RS16745 0.75 -0.9 1,529,954 + BBR_RS16745 0.90 -0.6 1,529,954 + BBR_RS16745 0.90 -0.6 1,529,954 + BBR_RS16745 0.90 -0.3 1,529,962 - +0.1 1,529,962 - -0.1 1,529,962 - -0.2 1,529,991 + -1.5 1,530,010 + -1.2 1,530,300 + BBR_RS16750 0.31 +0.3 1,530,322 + BBR_RS16750 0.33 +1.0 1,530,339 - BBR_RS16750 0.35 -1.1 1,530,339 - BBR_RS16750 0.35 -0.4 1,530,355 + BBR_RS16750 0.37 -0.0 1,530,374 + BBR_RS16750 0.40 +1.0 1,530,382 - BBR_RS16750 0.41 +0.3 1,530,478 - BBR_RS16750 0.52 +2.6 1,530,478 - BBR_RS16750 0.52 +0.6 1,530,506 - BBR_RS16750 0.55 -1.2 1,530,676 - BBR_RS16750 0.76 -0.4 1,530,745 + BBR_RS16750 0.84 +0.6 1,530,767 + BBR_RS16750 0.86 -0.0 1,530,783 + BBR_RS16750 0.88 -0.6 1,530,853 - +2.6 1,530,989 + -2.1 1,530,989 + +0.4 1,531,026 + -0.0 1,531,034 - -0.1 1,531,039 - +0.1 1,531,073 - +0.2 1,531,074 - +0.5 1,531,074 - +0.4 1,531,110 - +0.3 1,531,110 - +0.6 1,531,166 + +0.7 1,531,181 + -1.1 1,531,206 - BBR_RS16755 0.11 -0.0 1,531,217 + BBR_RS16755 0.12 +0.7 1,531,217 - BBR_RS16755 0.12 +0.6 1,531,225 - BBR_RS16755 0.13 -2.3 1,531,227 - BBR_RS16755 0.13 -1.0 1,531,269 + BBR_RS16755 0.18 -0.2 1,531,349 - BBR_RS16755 0.26 -1.9 1,531,431 - BBR_RS16755 0.35 -1.4 1,531,444 + BBR_RS16755 0.36 +0.6 1,531,479 + BBR_RS16755 0.40 -0.6 1,531,484 + BBR_RS16755 0.41 -0.2 1,531,499 + BBR_RS16755 0.42 +0.3 1,531,507 - BBR_RS16755 0.43 +0.2 1,531,520 + BBR_RS16755 0.45 -0.5 1,531,615 - BBR_RS16755 0.55 -0.7 1,531,687 + BBR_RS16755 0.63 +1.6 1,531,704 - BBR_RS16755 0.64 -0.4 1,531,847 + BBR_RS16755 0.80 -0.0
Or see this region's nucleotide sequence