Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16605 and BBR_RS16610 are separated by 64 nucleotides BBR_RS16610 and BBR_RS16615 are separated by 11 nucleotides
BBR_RS16605: BBR_RS16605 - hypothetical protein, at 1,493,206 to 1,495,818
_RS16605
BBR_RS16610: BBR_RS16610 - hypothetical protein, at 1,495,883 to 1,496,467
_RS16610
BBR_RS16615: BBR_RS16615 - class I SAM-dependent RNA methyltransferase, at 1,496,479 to 1,497,738
_RS16615
Position (kb)
1495
1496
1497 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1494.931 kb on - strand, within BBR_RS16605 at 1494.980 kb on + strand, within BBR_RS16605 at 1495.099 kb on - strand, within BBR_RS16605 at 1495.120 kb on + strand, within BBR_RS16605 at 1495.126 kb on - strand, within BBR_RS16605 at 1495.126 kb on - strand, within BBR_RS16605 at 1495.150 kb on - strand, within BBR_RS16605 at 1495.158 kb on - strand, within BBR_RS16605 at 1495.168 kb on + strand, within BBR_RS16605 at 1495.177 kb on - strand, within BBR_RS16605 at 1495.202 kb on + strand, within BBR_RS16605 at 1495.210 kb on - strand, within BBR_RS16605 at 1495.244 kb on + strand, within BBR_RS16605 at 1495.250 kb on + strand, within BBR_RS16605 at 1495.258 kb on - strand, within BBR_RS16605 at 1495.370 kb on + strand, within BBR_RS16605 at 1495.383 kb on - strand, within BBR_RS16605 at 1495.384 kb on - strand, within BBR_RS16605 at 1495.517 kb on + strand, within BBR_RS16605 at 1495.538 kb on + strand, within BBR_RS16605 at 1495.538 kb on + strand, within BBR_RS16605 at 1495.538 kb on + strand, within BBR_RS16605 at 1495.546 kb on - strand, within BBR_RS16605 at 1495.660 kb on - strand at 1495.687 kb on + strand at 1495.704 kb on + strand at 1495.772 kb on - strand at 1495.816 kb on + strand at 1495.819 kb on - strand at 1495.910 kb on + strand at 1495.910 kb on + strand at 1495.962 kb on + strand, within BBR_RS16610 at 1496.003 kb on + strand, within BBR_RS16610 at 1496.009 kb on - strand, within BBR_RS16610 at 1496.023 kb on - strand, within BBR_RS16610 at 1496.053 kb on - strand, within BBR_RS16610 at 1496.075 kb on + strand, within BBR_RS16610 at 1496.086 kb on + strand, within BBR_RS16610 at 1496.104 kb on - strand, within BBR_RS16610 at 1496.235 kb on + strand, within BBR_RS16610 at 1496.253 kb on - strand, within BBR_RS16610 at 1496.377 kb on - strand, within BBR_RS16610 at 1496.412 kb on + strand at 1496.472 kb on - strand at 1496.475 kb on + strand at 1496.561 kb on - strand at 1496.565 kb on + strand at 1496.569 kb on + strand at 1496.623 kb on - strand, within BBR_RS16615 at 1496.689 kb on + strand, within BBR_RS16615 at 1496.719 kb on + strand, within BBR_RS16615 at 1496.719 kb on + strand, within BBR_RS16615 at 1496.741 kb on + strand, within BBR_RS16615 at 1496.741 kb on + strand, within BBR_RS16615 at 1496.741 kb on + strand, within BBR_RS16615 at 1496.749 kb on - strand, within BBR_RS16615 at 1496.749 kb on - strand, within BBR_RS16615 at 1496.759 kb on - strand, within BBR_RS16615 at 1496.823 kb on + strand, within BBR_RS16615 at 1496.823 kb on + strand, within BBR_RS16615 at 1496.850 kb on - strand, within BBR_RS16615 at 1496.856 kb on + strand, within BBR_RS16615 at 1496.875 kb on - strand, within BBR_RS16615 at 1496.917 kb on + strand, within BBR_RS16615 at 1496.917 kb on + strand, within BBR_RS16615 at 1496.917 kb on + strand, within BBR_RS16615 at 1496.936 kb on + strand, within BBR_RS16615 at 1496.944 kb on - strand, within BBR_RS16615 at 1496.944 kb on - strand, within BBR_RS16615 at 1496.969 kb on + strand, within BBR_RS16615 at 1497.014 kb on + strand, within BBR_RS16615 at 1497.060 kb on + strand, within BBR_RS16615 at 1497.075 kb on + strand, within BBR_RS16615 at 1497.091 kb on - strand, within BBR_RS16615 at 1497.145 kb on + strand, within BBR_RS16615 at 1497.155 kb on + strand, within BBR_RS16615 at 1497.191 kb on - strand, within BBR_RS16615 at 1497.214 kb on - strand, within BBR_RS16615 at 1497.264 kb on - strand, within BBR_RS16615 at 1497.312 kb on + strand, within BBR_RS16615 at 1497.348 kb on - strand, within BBR_RS16615 at 1497.412 kb on - strand, within BBR_RS16615
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 1,494,931 - BBR_RS16605 0.66 +0.8 1,494,980 + BBR_RS16605 0.68 +1.0 1,495,099 - BBR_RS16605 0.72 +1.2 1,495,120 + BBR_RS16605 0.73 +1.5 1,495,126 - BBR_RS16605 0.73 +0.2 1,495,126 - BBR_RS16605 0.73 +0.8 1,495,150 - BBR_RS16605 0.74 +0.5 1,495,158 - BBR_RS16605 0.75 +0.3 1,495,168 + BBR_RS16605 0.75 -0.9 1,495,177 - BBR_RS16605 0.75 +0.6 1,495,202 + BBR_RS16605 0.76 +0.8 1,495,210 - BBR_RS16605 0.77 -0.1 1,495,244 + BBR_RS16605 0.78 +0.8 1,495,250 + BBR_RS16605 0.78 +0.4 1,495,258 - BBR_RS16605 0.79 +1.2 1,495,370 + BBR_RS16605 0.83 +1.0 1,495,383 - BBR_RS16605 0.83 +1.3 1,495,384 - BBR_RS16605 0.83 +1.4 1,495,517 + BBR_RS16605 0.88 -0.1 1,495,538 + BBR_RS16605 0.89 +0.8 1,495,538 + BBR_RS16605 0.89 +0.2 1,495,538 + BBR_RS16605 0.89 -1.2 1,495,546 - BBR_RS16605 0.90 +0.6 1,495,660 - -0.3 1,495,687 + -1.5 1,495,704 + +1.5 1,495,772 - +0.2 1,495,816 + +0.6 1,495,819 - -0.9 1,495,910 + -0.5 1,495,910 + -0.2 1,495,962 + BBR_RS16610 0.14 -1.1 1,496,003 + BBR_RS16610 0.21 -0.2 1,496,009 - BBR_RS16610 0.22 +1.2 1,496,023 - BBR_RS16610 0.24 -0.5 1,496,053 - BBR_RS16610 0.29 +1.1 1,496,075 + BBR_RS16610 0.33 -0.3 1,496,086 + BBR_RS16610 0.35 -0.3 1,496,104 - BBR_RS16610 0.38 -0.4 1,496,235 + BBR_RS16610 0.60 -2.1 1,496,253 - BBR_RS16610 0.63 -0.6 1,496,377 - BBR_RS16610 0.84 -1.1 1,496,412 + -0.6 1,496,472 - +0.7 1,496,475 + -0.3 1,496,561 - +0.7 1,496,565 + -1.1 1,496,569 + -0.1 1,496,623 - BBR_RS16615 0.11 -0.3 1,496,689 + BBR_RS16615 0.17 +1.5 1,496,719 + BBR_RS16615 0.19 -0.8 1,496,719 + BBR_RS16615 0.19 -0.4 1,496,741 + BBR_RS16615 0.21 +0.8 1,496,741 + BBR_RS16615 0.21 -0.9 1,496,741 + BBR_RS16615 0.21 +0.6 1,496,749 - BBR_RS16615 0.21 -0.3 1,496,749 - BBR_RS16615 0.21 -1.0 1,496,759 - BBR_RS16615 0.22 -0.0 1,496,823 + BBR_RS16615 0.27 -0.3 1,496,823 + BBR_RS16615 0.27 +0.8 1,496,850 - BBR_RS16615 0.29 -0.2 1,496,856 + BBR_RS16615 0.30 -0.6 1,496,875 - BBR_RS16615 0.31 -0.3 1,496,917 + BBR_RS16615 0.35 -1.5 1,496,917 + BBR_RS16615 0.35 +0.4 1,496,917 + BBR_RS16615 0.35 +0.5 1,496,936 + BBR_RS16615 0.36 +3.5 1,496,944 - BBR_RS16615 0.37 -0.6 1,496,944 - BBR_RS16615 0.37 +0.4 1,496,969 + BBR_RS16615 0.39 -0.7 1,497,014 + BBR_RS16615 0.42 +0.0 1,497,060 + BBR_RS16615 0.46 -1.0 1,497,075 + BBR_RS16615 0.47 -0.2 1,497,091 - BBR_RS16615 0.49 -0.0 1,497,145 + BBR_RS16615 0.53 -0.6 1,497,155 + BBR_RS16615 0.54 -0.0 1,497,191 - BBR_RS16615 0.57 -0.2 1,497,214 - BBR_RS16615 0.58 -1.1 1,497,264 - BBR_RS16615 0.62 -0.0 1,497,312 + BBR_RS16615 0.66 -1.3 1,497,348 - BBR_RS16615 0.69 -1.9 1,497,412 - BBR_RS16615 0.74 -0.0
Or see this region's nucleotide sequence