Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16245 and BBR_RS16250 are separated by 21 nucleotides BBR_RS16250 and BBR_RS16255 are separated by 56 nucleotides BBR_RS16255 and BBR_RS16260 are separated by 8 nucleotides
BBR_RS16245: BBR_RS16245 - ribosomal-protein-alanine N-acetyltransferase, at 1,391,508 to 1,392,107
_RS16245
BBR_RS16250: BBR_RS16250 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB, at 1,392,129 to 1,393,010
_RS16250
BBR_RS16255: BBR_RS16255 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE, at 1,393,067 to 1,393,633
_RS16255
BBR_RS16260: BBR_RS16260 - DNA polymerase III subunit delta, at 1,393,642 to 1,394,613
_RS16260
Position (kb)
1393
1394 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1392.157 kb on + strand at 1392.182 kb on - strand at 1392.212 kb on - strand at 1392.235 kb on - strand, within BBR_RS16250 at 1392.296 kb on + strand, within BBR_RS16250 at 1392.307 kb on - strand, within BBR_RS16250 at 1392.373 kb on - strand, within BBR_RS16250 at 1392.452 kb on + strand, within BBR_RS16250 at 1392.581 kb on - strand, within BBR_RS16250 at 1392.581 kb on - strand, within BBR_RS16250 at 1392.829 kb on - strand, within BBR_RS16250 at 1392.903 kb on - strand, within BBR_RS16250 at 1392.946 kb on - strand at 1393.030 kb on + strand at 1393.030 kb on + strand at 1393.030 kb on + strand at 1393.030 kb on + strand at 1393.030 kb on + strand at 1393.038 kb on - strand at 1393.038 kb on - strand at 1393.038 kb on - strand at 1393.038 kb on - strand at 1393.038 kb on - strand at 1393.038 kb on - strand at 1393.038 kb on - strand at 1393.038 kb on - strand at 1393.038 kb on - strand at 1393.038 kb on - strand at 1393.038 kb on - strand at 1393.040 kb on - strand at 1393.073 kb on - strand at 1393.113 kb on - strand at 1393.193 kb on - strand, within BBR_RS16255 at 1393.209 kb on - strand, within BBR_RS16255 at 1393.239 kb on - strand, within BBR_RS16255 at 1393.273 kb on - strand, within BBR_RS16255 at 1393.388 kb on + strand, within BBR_RS16255 at 1393.488 kb on - strand, within BBR_RS16255 at 1393.528 kb on - strand, within BBR_RS16255 at 1393.538 kb on + strand, within BBR_RS16255 at 1393.621 kb on + strand at 1393.629 kb on - strand at 1393.658 kb on - strand at 1393.658 kb on - strand at 1394.590 kb on - strand at 1394.622 kb on - strand at 1394.622 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 1,392,157 + -0.8 1,392,182 - +0.9 1,392,212 - +0.0 1,392,235 - BBR_RS16250 0.12 +1.0 1,392,296 + BBR_RS16250 0.19 +0.5 1,392,307 - BBR_RS16250 0.20 -0.8 1,392,373 - BBR_RS16250 0.28 +0.8 1,392,452 + BBR_RS16250 0.37 +0.4 1,392,581 - BBR_RS16250 0.51 -1.9 1,392,581 - BBR_RS16250 0.51 -1.1 1,392,829 - BBR_RS16250 0.79 +0.3 1,392,903 - BBR_RS16250 0.88 +2.5 1,392,946 - +0.2 1,393,030 + +1.5 1,393,030 + +1.0 1,393,030 + +1.1 1,393,030 + +2.8 1,393,030 + -0.4 1,393,038 - -0.3 1,393,038 - +0.2 1,393,038 - +0.4 1,393,038 - -0.7 1,393,038 - +0.0 1,393,038 - +0.8 1,393,038 - +0.1 1,393,038 - +0.5 1,393,038 - -0.4 1,393,038 - -1.3 1,393,038 - +0.9 1,393,040 - +1.4 1,393,073 - +0.7 1,393,113 - +1.0 1,393,193 - BBR_RS16255 0.22 -0.3 1,393,209 - BBR_RS16255 0.25 +1.1 1,393,239 - BBR_RS16255 0.30 -0.1 1,393,273 - BBR_RS16255 0.36 +1.9 1,393,388 + BBR_RS16255 0.57 +0.6 1,393,488 - BBR_RS16255 0.74 -0.1 1,393,528 - BBR_RS16255 0.81 -1.3 1,393,538 + BBR_RS16255 0.83 -0.5 1,393,621 + +0.1 1,393,629 - +2.5 1,393,658 - -0.3 1,393,658 - +0.0 1,394,590 - +0.0 1,394,622 - +0.9 1,394,622 - +0.4
Or see this region's nucleotide sequence