Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16240 and BBR_RS16245 overlap by 4 nucleotides BBR_RS16245 and BBR_RS16250 are separated by 21 nucleotides BBR_RS16250 and BBR_RS16255 are separated by 56 nucleotides
BBR_RS16240: BBR_RS16240 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD, at 1,390,468 to 1,391,511
_RS16240
BBR_RS16245: BBR_RS16245 - ribosomal-protein-alanine N-acetyltransferase, at 1,391,508 to 1,392,107
_RS16245
BBR_RS16250: BBR_RS16250 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB, at 1,392,129 to 1,393,010
_RS16250
BBR_RS16255: BBR_RS16255 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE, at 1,393,067 to 1,393,633
_RS16255
Position (kb)
1391
1392
1393 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1390.527 kb on + strand at 1390.795 kb on + strand, within BBR_RS16240 at 1390.886 kb on + strand, within BBR_RS16240 at 1390.894 kb on - strand, within BBR_RS16240 at 1390.897 kb on + strand, within BBR_RS16240 at 1390.971 kb on + strand, within BBR_RS16240 at 1391.021 kb on - strand, within BBR_RS16240 at 1391.021 kb on - strand, within BBR_RS16240 at 1391.021 kb on - strand, within BBR_RS16240 at 1391.066 kb on + strand, within BBR_RS16240 at 1391.074 kb on - strand, within BBR_RS16240 at 1391.087 kb on - strand, within BBR_RS16240 at 1391.217 kb on - strand, within BBR_RS16240 at 1391.256 kb on + strand, within BBR_RS16240 at 1391.264 kb on - strand, within BBR_RS16240 at 1391.266 kb on + strand, within BBR_RS16240 at 1391.307 kb on - strand, within BBR_RS16240 at 1391.394 kb on - strand, within BBR_RS16240 at 1391.434 kb on - strand at 1391.488 kb on + strand at 1391.501 kb on - strand at 1391.501 kb on - strand at 1391.548 kb on - strand at 1391.548 kb on - strand at 1391.548 kb on - strand at 1391.649 kb on - strand, within BBR_RS16245 at 1391.711 kb on + strand, within BBR_RS16245 at 1391.714 kb on - strand, within BBR_RS16245 at 1391.782 kb on - strand, within BBR_RS16245 at 1391.871 kb on - strand, within BBR_RS16245 at 1391.972 kb on - strand, within BBR_RS16245 at 1392.038 kb on - strand, within BBR_RS16245 at 1392.051 kb on - strand at 1392.157 kb on + strand at 1392.182 kb on - strand at 1392.212 kb on - strand at 1392.235 kb on - strand, within BBR_RS16250 at 1392.296 kb on + strand, within BBR_RS16250 at 1392.307 kb on - strand, within BBR_RS16250 at 1392.373 kb on - strand, within BBR_RS16250 at 1392.452 kb on + strand, within BBR_RS16250 at 1392.581 kb on - strand, within BBR_RS16250 at 1392.581 kb on - strand, within BBR_RS16250 at 1392.829 kb on - strand, within BBR_RS16250 at 1392.903 kb on - strand, within BBR_RS16250 at 1392.946 kb on - strand at 1393.030 kb on + strand at 1393.030 kb on + strand at 1393.030 kb on + strand at 1393.030 kb on + strand at 1393.030 kb on + strand at 1393.038 kb on - strand at 1393.038 kb on - strand at 1393.038 kb on - strand at 1393.038 kb on - strand at 1393.038 kb on - strand at 1393.038 kb on - strand at 1393.038 kb on - strand at 1393.038 kb on - strand at 1393.038 kb on - strand at 1393.038 kb on - strand at 1393.038 kb on - strand at 1393.040 kb on - strand at 1393.073 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 1,390,527 + +2.5 1,390,795 + BBR_RS16240 0.31 +0.3 1,390,886 + BBR_RS16240 0.40 -0.0 1,390,894 - BBR_RS16240 0.41 +0.6 1,390,897 + BBR_RS16240 0.41 +0.1 1,390,971 + BBR_RS16240 0.48 -0.3 1,391,021 - BBR_RS16240 0.53 +1.0 1,391,021 - BBR_RS16240 0.53 -1.0 1,391,021 - BBR_RS16240 0.53 +1.1 1,391,066 + BBR_RS16240 0.57 +0.7 1,391,074 - BBR_RS16240 0.58 +0.7 1,391,087 - BBR_RS16240 0.59 +0.4 1,391,217 - BBR_RS16240 0.72 +2.0 1,391,256 + BBR_RS16240 0.75 +0.3 1,391,264 - BBR_RS16240 0.76 +0.0 1,391,266 + BBR_RS16240 0.76 +1.5 1,391,307 - BBR_RS16240 0.80 +0.4 1,391,394 - BBR_RS16240 0.89 -1.1 1,391,434 - -1.1 1,391,488 + -0.3 1,391,501 - +0.1 1,391,501 - -0.3 1,391,548 - -0.4 1,391,548 - +0.0 1,391,548 - -0.1 1,391,649 - BBR_RS16245 0.23 +0.6 1,391,711 + BBR_RS16245 0.34 -0.3 1,391,714 - BBR_RS16245 0.34 +0.3 1,391,782 - BBR_RS16245 0.46 -1.4 1,391,871 - BBR_RS16245 0.60 +1.7 1,391,972 - BBR_RS16245 0.77 -1.2 1,392,038 - BBR_RS16245 0.88 -1.4 1,392,051 - +1.7 1,392,157 + -0.8 1,392,182 - +0.9 1,392,212 - +0.0 1,392,235 - BBR_RS16250 0.12 +1.0 1,392,296 + BBR_RS16250 0.19 +0.5 1,392,307 - BBR_RS16250 0.20 -0.8 1,392,373 - BBR_RS16250 0.28 +0.8 1,392,452 + BBR_RS16250 0.37 +0.4 1,392,581 - BBR_RS16250 0.51 -1.9 1,392,581 - BBR_RS16250 0.51 -1.1 1,392,829 - BBR_RS16250 0.79 +0.3 1,392,903 - BBR_RS16250 0.88 +2.5 1,392,946 - +0.2 1,393,030 + +1.5 1,393,030 + +1.0 1,393,030 + +1.1 1,393,030 + +2.8 1,393,030 + -0.4 1,393,038 - -0.3 1,393,038 - +0.2 1,393,038 - +0.4 1,393,038 - -0.7 1,393,038 - +0.0 1,393,038 - +0.8 1,393,038 - +0.1 1,393,038 - +0.5 1,393,038 - -0.4 1,393,038 - -1.3 1,393,038 - +0.9 1,393,040 - +1.4 1,393,073 - +0.7
Or see this region's nucleotide sequence