Strain Fitness in Bifidobacterium breve UCC2003 around BBR_RS16245

Experiment: Recovery broth with Tiamulin 2e-04 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBBR_RS16240 and BBR_RS16245 overlap by 4 nucleotidesBBR_RS16245 and BBR_RS16250 are separated by 21 nucleotidesBBR_RS16250 and BBR_RS16255 are separated by 56 nucleotides BBR_RS16240: BBR_RS16240 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD, at 1,390,468 to 1,391,511 _RS16240 BBR_RS16245: BBR_RS16245 - ribosomal-protein-alanine N-acetyltransferase, at 1,391,508 to 1,392,107 _RS16245 BBR_RS16250: BBR_RS16250 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB, at 1,392,129 to 1,393,010 _RS16250 BBR_RS16255: BBR_RS16255 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE, at 1,393,067 to 1,393,633 _RS16255 Position (kb) 1391 1392 1393Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1390.527 kb on + strandat 1390.795 kb on + strand, within BBR_RS16240at 1390.886 kb on + strand, within BBR_RS16240at 1390.894 kb on - strand, within BBR_RS16240at 1390.897 kb on + strand, within BBR_RS16240at 1390.971 kb on + strand, within BBR_RS16240at 1391.021 kb on - strand, within BBR_RS16240at 1391.021 kb on - strand, within BBR_RS16240at 1391.021 kb on - strand, within BBR_RS16240at 1391.066 kb on + strand, within BBR_RS16240at 1391.074 kb on - strand, within BBR_RS16240at 1391.087 kb on - strand, within BBR_RS16240at 1391.217 kb on - strand, within BBR_RS16240at 1391.256 kb on + strand, within BBR_RS16240at 1391.264 kb on - strand, within BBR_RS16240at 1391.266 kb on + strand, within BBR_RS16240at 1391.307 kb on - strand, within BBR_RS16240at 1391.394 kb on - strand, within BBR_RS16240at 1391.434 kb on - strandat 1391.488 kb on + strandat 1391.501 kb on - strandat 1391.501 kb on - strandat 1391.548 kb on - strandat 1391.548 kb on - strandat 1391.548 kb on - strandat 1391.649 kb on - strand, within BBR_RS16245at 1391.711 kb on + strand, within BBR_RS16245at 1391.714 kb on - strand, within BBR_RS16245at 1391.782 kb on - strand, within BBR_RS16245at 1391.871 kb on - strand, within BBR_RS16245at 1391.972 kb on - strand, within BBR_RS16245at 1392.038 kb on - strand, within BBR_RS16245at 1392.051 kb on - strandat 1392.157 kb on + strandat 1392.182 kb on - strandat 1392.212 kb on - strandat 1392.235 kb on - strand, within BBR_RS16250at 1392.296 kb on + strand, within BBR_RS16250at 1392.307 kb on - strand, within BBR_RS16250at 1392.373 kb on - strand, within BBR_RS16250at 1392.452 kb on + strand, within BBR_RS16250at 1392.581 kb on - strand, within BBR_RS16250at 1392.581 kb on - strand, within BBR_RS16250at 1392.829 kb on - strand, within BBR_RS16250at 1392.903 kb on - strand, within BBR_RS16250at 1392.946 kb on - strandat 1393.030 kb on + strandat 1393.030 kb on + strandat 1393.030 kb on + strandat 1393.030 kb on + strandat 1393.030 kb on + strandat 1393.038 kb on - strandat 1393.038 kb on - strandat 1393.038 kb on - strandat 1393.038 kb on - strandat 1393.038 kb on - strandat 1393.038 kb on - strandat 1393.038 kb on - strandat 1393.038 kb on - strandat 1393.038 kb on - strandat 1393.038 kb on - strandat 1393.038 kb on - strandat 1393.040 kb on - strandat 1393.073 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM
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1,390,527 + +2.5
1,390,795 + BBR_RS16240 0.31 +0.3
1,390,886 + BBR_RS16240 0.40 -0.0
1,390,894 - BBR_RS16240 0.41 +0.6
1,390,897 + BBR_RS16240 0.41 +0.1
1,390,971 + BBR_RS16240 0.48 -0.3
1,391,021 - BBR_RS16240 0.53 +1.0
1,391,021 - BBR_RS16240 0.53 -1.0
1,391,021 - BBR_RS16240 0.53 +1.1
1,391,066 + BBR_RS16240 0.57 +0.7
1,391,074 - BBR_RS16240 0.58 +0.7
1,391,087 - BBR_RS16240 0.59 +0.4
1,391,217 - BBR_RS16240 0.72 +2.0
1,391,256 + BBR_RS16240 0.75 +0.3
1,391,264 - BBR_RS16240 0.76 +0.0
1,391,266 + BBR_RS16240 0.76 +1.5
1,391,307 - BBR_RS16240 0.80 +0.4
1,391,394 - BBR_RS16240 0.89 -1.1
1,391,434 - -1.1
1,391,488 + -0.3
1,391,501 - +0.1
1,391,501 - -0.3
1,391,548 - -0.4
1,391,548 - +0.0
1,391,548 - -0.1
1,391,649 - BBR_RS16245 0.23 +0.6
1,391,711 + BBR_RS16245 0.34 -0.3
1,391,714 - BBR_RS16245 0.34 +0.3
1,391,782 - BBR_RS16245 0.46 -1.4
1,391,871 - BBR_RS16245 0.60 +1.7
1,391,972 - BBR_RS16245 0.77 -1.2
1,392,038 - BBR_RS16245 0.88 -1.4
1,392,051 - +1.7
1,392,157 + -0.8
1,392,182 - +0.9
1,392,212 - +0.0
1,392,235 - BBR_RS16250 0.12 +1.0
1,392,296 + BBR_RS16250 0.19 +0.5
1,392,307 - BBR_RS16250 0.20 -0.8
1,392,373 - BBR_RS16250 0.28 +0.8
1,392,452 + BBR_RS16250 0.37 +0.4
1,392,581 - BBR_RS16250 0.51 -1.9
1,392,581 - BBR_RS16250 0.51 -1.1
1,392,829 - BBR_RS16250 0.79 +0.3
1,392,903 - BBR_RS16250 0.88 +2.5
1,392,946 - +0.2
1,393,030 + +1.5
1,393,030 + +1.0
1,393,030 + +1.1
1,393,030 + +2.8
1,393,030 + -0.4
1,393,038 - -0.3
1,393,038 - +0.2
1,393,038 - +0.4
1,393,038 - -0.7
1,393,038 - +0.0
1,393,038 - +0.8
1,393,038 - +0.1
1,393,038 - +0.5
1,393,038 - -0.4
1,393,038 - -1.3
1,393,038 - +0.9
1,393,040 - +1.4
1,393,073 - +0.7

Or see this region's nucleotide sequence