Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15940 and BBR_RS15945 overlap by 4 nucleotides BBR_RS15945 and BBR_RS15950 are separated by 51 nucleotides BBR_RS15950 and BBR_RS15955 are separated by 53 nucleotides BBR_RS15955 and BBR_RS15960 are separated by 94 nucleotides
BBR_RS15940: BBR_RS15940 - hypothetical protein, at 1,335,566 to 1,336,192
_RS15940
BBR_RS15945: BBR_RS15945 - hypothetical protein, at 1,336,189 to 1,336,971
_RS15945
BBR_RS15950: BBR_RS15950 - ABC transporter ATP-binding protein, at 1,337,023 to 1,337,898
_RS15950
BBR_RS15955: BBR_RS15955 - hypothetical protein, at 1,337,952 to 1,338,350
_RS15955
BBR_RS15960: BBR_RS15960 - hypothetical protein, at 1,338,445 to 1,338,807
_RS15960
Position (kb)
1337
1338 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1336.144 kb on + strand at 1336.193 kb on - strand at 1336.302 kb on - strand, within BBR_RS15945 at 1336.413 kb on + strand, within BBR_RS15945 at 1336.413 kb on + strand, within BBR_RS15945 at 1336.413 kb on + strand, within BBR_RS15945 at 1336.431 kb on - strand, within BBR_RS15945 at 1336.431 kb on - strand, within BBR_RS15945 at 1336.450 kb on + strand, within BBR_RS15945 at 1336.450 kb on + strand, within BBR_RS15945 at 1336.466 kb on - strand, within BBR_RS15945 at 1336.466 kb on - strand, within BBR_RS15945 at 1336.477 kb on + strand, within BBR_RS15945 at 1336.623 kb on + strand, within BBR_RS15945 at 1336.633 kb on + strand, within BBR_RS15945 at 1336.726 kb on + strand, within BBR_RS15945 at 1336.905 kb on + strand at 1336.915 kb on + strand at 1336.935 kb on - strand at 1336.941 kb on + strand at 1336.941 kb on + strand at 1336.949 kb on - strand at 1336.953 kb on + strand at 1336.959 kb on - strand at 1336.961 kb on - strand at 1336.970 kb on + strand at 1337.090 kb on + strand at 1337.106 kb on + strand at 1337.186 kb on + strand, within BBR_RS15950 at 1337.223 kb on + strand, within BBR_RS15950 at 1337.294 kb on + strand, within BBR_RS15950 at 1337.295 kb on + strand, within BBR_RS15950 at 1337.396 kb on + strand, within BBR_RS15950 at 1337.464 kb on + strand, within BBR_RS15950 at 1337.511 kb on + strand, within BBR_RS15950 at 1337.617 kb on + strand, within BBR_RS15950 at 1337.617 kb on + strand, within BBR_RS15950 at 1337.617 kb on + strand, within BBR_RS15950 at 1337.653 kb on + strand, within BBR_RS15950 at 1337.653 kb on + strand, within BBR_RS15950 at 1337.653 kb on + strand, within BBR_RS15950 at 1337.653 kb on + strand, within BBR_RS15950 at 1337.685 kb on - strand, within BBR_RS15950 at 1337.735 kb on - strand, within BBR_RS15950 at 1337.743 kb on - strand, within BBR_RS15950 at 1337.786 kb on + strand, within BBR_RS15950 at 1337.794 kb on - strand, within BBR_RS15950 at 1337.794 kb on - strand, within BBR_RS15950 at 1337.882 kb on + strand at 1337.900 kb on - strand at 1337.973 kb on + strand at 1337.981 kb on - strand at 1338.013 kb on - strand, within BBR_RS15955 at 1338.031 kb on + strand, within BBR_RS15955 at 1338.039 kb on - strand, within BBR_RS15955 at 1338.048 kb on - strand, within BBR_RS15955 at 1338.048 kb on - strand, within BBR_RS15955 at 1338.048 kb on - strand, within BBR_RS15955 at 1338.059 kb on - strand, within BBR_RS15955 at 1338.102 kb on + strand, within BBR_RS15955 at 1338.108 kb on - strand, within BBR_RS15955 at 1338.108 kb on - strand, within BBR_RS15955 at 1338.115 kb on - strand, within BBR_RS15955 at 1338.236 kb on - strand, within BBR_RS15955 at 1338.243 kb on - strand, within BBR_RS15955 at 1338.276 kb on + strand, within BBR_RS15955 at 1338.317 kb on - strand at 1338.444 kb on - strand at 1338.495 kb on - strand, within BBR_RS15960 at 1338.577 kb on - strand, within BBR_RS15960 at 1338.577 kb on - strand, within BBR_RS15960 at 1338.596 kb on - strand, within BBR_RS15960 at 1338.635 kb on + strand, within BBR_RS15960 at 1338.635 kb on + strand, within BBR_RS15960 at 1338.635 kb on + strand, within BBR_RS15960 at 1338.635 kb on + strand, within BBR_RS15960 at 1338.678 kb on + strand, within BBR_RS15960 at 1338.679 kb on - strand, within BBR_RS15960 at 1338.681 kb on + strand, within BBR_RS15960 at 1338.689 kb on - strand, within BBR_RS15960 at 1338.689 kb on - strand, within BBR_RS15960 at 1338.824 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 1,336,144 + -0.8 1,336,193 - -0.2 1,336,302 - BBR_RS15945 0.14 -0.1 1,336,413 + BBR_RS15945 0.29 -0.9 1,336,413 + BBR_RS15945 0.29 +0.0 1,336,413 + BBR_RS15945 0.29 +0.3 1,336,431 - BBR_RS15945 0.31 +0.6 1,336,431 - BBR_RS15945 0.31 +0.6 1,336,450 + BBR_RS15945 0.33 +0.3 1,336,450 + BBR_RS15945 0.33 +0.8 1,336,466 - BBR_RS15945 0.35 +0.0 1,336,466 - BBR_RS15945 0.35 -0.4 1,336,477 + BBR_RS15945 0.37 -2.1 1,336,623 + BBR_RS15945 0.55 +2.0 1,336,633 + BBR_RS15945 0.57 -1.1 1,336,726 + BBR_RS15945 0.69 -0.6 1,336,905 + +0.8 1,336,915 + +0.1 1,336,935 - +0.7 1,336,941 + +0.8 1,336,941 + -1.9 1,336,949 - +0.8 1,336,953 + -1.2 1,336,959 - -0.5 1,336,961 - -1.0 1,336,970 + -2.4 1,337,090 + +1.2 1,337,106 + -3.8 1,337,186 + BBR_RS15950 0.19 -0.8 1,337,223 + BBR_RS15950 0.23 -0.3 1,337,294 + BBR_RS15950 0.31 -1.4 1,337,295 + BBR_RS15950 0.31 +0.5 1,337,396 + BBR_RS15950 0.43 -1.1 1,337,464 + BBR_RS15950 0.50 -1.7 1,337,511 + BBR_RS15950 0.56 -0.7 1,337,617 + BBR_RS15950 0.68 -2.7 1,337,617 + BBR_RS15950 0.68 -1.7 1,337,617 + BBR_RS15950 0.68 -0.7 1,337,653 + BBR_RS15950 0.72 -0.4 1,337,653 + BBR_RS15950 0.72 -0.6 1,337,653 + BBR_RS15950 0.72 -0.6 1,337,653 + BBR_RS15950 0.72 +0.3 1,337,685 - BBR_RS15950 0.76 +0.6 1,337,735 - BBR_RS15950 0.81 -0.1 1,337,743 - BBR_RS15950 0.82 +0.0 1,337,786 + BBR_RS15950 0.87 +0.2 1,337,794 - BBR_RS15950 0.88 +0.0 1,337,794 - BBR_RS15950 0.88 -0.2 1,337,882 + +0.5 1,337,900 - -0.0 1,337,973 + +1.4 1,337,981 - -0.1 1,338,013 - BBR_RS15955 0.15 +0.1 1,338,031 + BBR_RS15955 0.20 +0.1 1,338,039 - BBR_RS15955 0.22 -0.4 1,338,048 - BBR_RS15955 0.24 -0.1 1,338,048 - BBR_RS15955 0.24 +1.7 1,338,048 - BBR_RS15955 0.24 -0.5 1,338,059 - BBR_RS15955 0.27 -0.3 1,338,102 + BBR_RS15955 0.38 +0.4 1,338,108 - BBR_RS15955 0.39 +1.4 1,338,108 - BBR_RS15955 0.39 +0.5 1,338,115 - BBR_RS15955 0.41 -0.3 1,338,236 - BBR_RS15955 0.71 -0.5 1,338,243 - BBR_RS15955 0.73 -0.3 1,338,276 + BBR_RS15955 0.81 -0.1 1,338,317 - +0.7 1,338,444 - +1.0 1,338,495 - BBR_RS15960 0.14 +0.2 1,338,577 - BBR_RS15960 0.36 +0.0 1,338,577 - BBR_RS15960 0.36 +0.0 1,338,596 - BBR_RS15960 0.42 +0.0 1,338,635 + BBR_RS15960 0.52 -0.6 1,338,635 + BBR_RS15960 0.52 -0.5 1,338,635 + BBR_RS15960 0.52 +0.2 1,338,635 + BBR_RS15960 0.52 -1.3 1,338,678 + BBR_RS15960 0.64 -0.6 1,338,679 - BBR_RS15960 0.64 -0.9 1,338,681 + BBR_RS15960 0.65 +0.3 1,338,689 - BBR_RS15960 0.67 -0.8 1,338,689 - BBR_RS15960 0.67 +1.7 1,338,824 - +0.5
Or see this region's nucleotide sequence