Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15795 and BBR_RS15800 are separated by 49 nucleotides BBR_RS15800 and BBR_RS15805 are separated by 79 nucleotides BBR_RS15805 and BBR_RS15810 are separated by 234 nucleotides
BBR_RS15795: BBR_RS15795 - phosphoribosyl-ATP diphosphatase, at 1,309,602 to 1,309,865
_RS15795
BBR_RS15800: BBR_RS15800 - ribulose-phosphate 3-epimerase, at 1,309,915 to 1,310,583
_RS15800
BBR_RS15805: BBR_RS15805 - prolipoprotein diacylglyceryl transferase, at 1,310,663 to 1,311,613
_RS15805
BBR_RS15810: BBR_RS15810 - tryptophan synthase subunit alpha, at 1,311,848 to 1,312,717
_RS15810
Position (kb)
1310
1311
1312 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1309.663 kb on + strand, within BBR_RS15795 at 1309.705 kb on + strand, within BBR_RS15795 at 1309.715 kb on - strand, within BBR_RS15795 at 1309.715 kb on - strand, within BBR_RS15795 at 1309.903 kb on - strand at 1309.913 kb on + strand at 1309.921 kb on - strand at 1309.926 kb on - strand at 1309.937 kb on + strand at 1310.599 kb on - strand at 1310.635 kb on + strand at 1310.635 kb on + strand at 1310.646 kb on + strand at 1310.717 kb on + strand at 1310.725 kb on - strand at 1310.747 kb on - strand at 1310.762 kb on + strand, within BBR_RS15805 at 1310.763 kb on + strand, within BBR_RS15805 at 1310.817 kb on - strand, within BBR_RS15805 at 1310.817 kb on - strand, within BBR_RS15805 at 1310.870 kb on - strand, within BBR_RS15805 at 1310.912 kb on + strand, within BBR_RS15805 at 1310.917 kb on + strand, within BBR_RS15805 at 1310.925 kb on - strand, within BBR_RS15805 at 1310.953 kb on - strand, within BBR_RS15805 at 1310.955 kb on - strand, within BBR_RS15805 at 1310.963 kb on - strand, within BBR_RS15805 at 1310.963 kb on - strand, within BBR_RS15805 at 1310.963 kb on - strand, within BBR_RS15805 at 1310.963 kb on - strand, within BBR_RS15805 at 1310.963 kb on - strand, within BBR_RS15805 at 1311.042 kb on - strand, within BBR_RS15805 at 1311.135 kb on + strand, within BBR_RS15805 at 1311.135 kb on - strand, within BBR_RS15805 at 1311.143 kb on - strand, within BBR_RS15805 at 1311.143 kb on - strand, within BBR_RS15805 at 1311.155 kb on - strand, within BBR_RS15805 at 1311.166 kb on - strand, within BBR_RS15805 at 1311.215 kb on - strand, within BBR_RS15805 at 1311.407 kb on - strand, within BBR_RS15805 at 1311.445 kb on - strand, within BBR_RS15805 at 1311.503 kb on + strand, within BBR_RS15805 at 1311.545 kb on + strand at 1311.691 kb on + strand at 1311.691 kb on + strand at 1311.716 kb on - strand at 1311.718 kb on - strand at 1311.726 kb on + strand at 1311.739 kb on + strand at 1311.741 kb on - strand at 1311.741 kb on - strand at 1311.844 kb on + strand at 1311.853 kb on - strand at 1311.877 kb on + strand at 1311.899 kb on - strand at 1311.910 kb on - strand at 1311.921 kb on - strand at 1311.930 kb on + strand at 1311.930 kb on + strand at 1311.952 kb on + strand, within BBR_RS15810 at 1311.952 kb on + strand, within BBR_RS15810 at 1311.954 kb on + strand, within BBR_RS15810 at 1311.960 kb on - strand, within BBR_RS15810 at 1311.960 kb on - strand, within BBR_RS15810 at 1311.962 kb on - strand, within BBR_RS15810 at 1311.972 kb on - strand, within BBR_RS15810 at 1312.021 kb on - strand, within BBR_RS15810 at 1312.061 kb on + strand, within BBR_RS15810 at 1312.061 kb on + strand, within BBR_RS15810 at 1312.069 kb on - strand, within BBR_RS15810 at 1312.069 kb on - strand, within BBR_RS15810 at 1312.093 kb on - strand, within BBR_RS15810 at 1312.097 kb on + strand, within BBR_RS15810 at 1312.098 kb on + strand, within BBR_RS15810 at 1312.176 kb on + strand, within BBR_RS15810 at 1312.184 kb on - strand, within BBR_RS15810 at 1312.184 kb on - strand, within BBR_RS15810 at 1312.198 kb on + strand, within BBR_RS15810 at 1312.206 kb on - strand, within BBR_RS15810 at 1312.206 kb on - strand, within BBR_RS15810 at 1312.206 kb on - strand, within BBR_RS15810 at 1312.206 kb on - strand, within BBR_RS15810 at 1312.211 kb on - strand, within BBR_RS15810 at 1312.253 kb on - strand, within BBR_RS15810 at 1312.349 kb on - strand, within BBR_RS15810 at 1312.357 kb on + strand, within BBR_RS15810 at 1312.448 kb on - strand, within BBR_RS15810 at 1312.484 kb on + strand, within BBR_RS15810 at 1312.495 kb on + strand, within BBR_RS15810 at 1312.500 kb on + strand, within BBR_RS15810 at 1312.500 kb on + strand, within BBR_RS15810 at 1312.503 kb on - strand, within BBR_RS15810 at 1312.508 kb on - strand, within BBR_RS15810 at 1312.508 kb on - strand, within BBR_RS15810 at 1312.578 kb on - strand, within BBR_RS15810 at 1312.578 kb on - strand, within BBR_RS15810 at 1312.595 kb on - strand, within BBR_RS15810
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 1,309,663 + BBR_RS15795 0.23 +0.1 1,309,705 + BBR_RS15795 0.39 -0.0 1,309,715 - BBR_RS15795 0.43 -1.4 1,309,715 - BBR_RS15795 0.43 -1.6 1,309,903 - -0.1 1,309,913 + +0.1 1,309,921 - +0.5 1,309,926 - -0.2 1,309,937 + +0.0 1,310,599 - +1.4 1,310,635 + +0.6 1,310,635 + -0.3 1,310,646 + -1.5 1,310,717 + -0.5 1,310,725 - -2.3 1,310,747 - +0.5 1,310,762 + BBR_RS15805 0.10 -1.0 1,310,763 + BBR_RS15805 0.11 +0.7 1,310,817 - BBR_RS15805 0.16 +0.4 1,310,817 - BBR_RS15805 0.16 -0.2 1,310,870 - BBR_RS15805 0.22 -1.2 1,310,912 + BBR_RS15805 0.26 +0.1 1,310,917 + BBR_RS15805 0.27 +3.4 1,310,925 - BBR_RS15805 0.28 -1.1 1,310,953 - BBR_RS15805 0.30 -0.6 1,310,955 - BBR_RS15805 0.31 -0.6 1,310,963 - BBR_RS15805 0.32 +0.1 1,310,963 - BBR_RS15805 0.32 -1.5 1,310,963 - BBR_RS15805 0.32 -1.6 1,310,963 - BBR_RS15805 0.32 -1.6 1,310,963 - BBR_RS15805 0.32 +1.4 1,311,042 - BBR_RS15805 0.40 -1.4 1,311,135 + BBR_RS15805 0.50 +0.3 1,311,135 - BBR_RS15805 0.50 -2.6 1,311,143 - BBR_RS15805 0.50 -1.1 1,311,143 - BBR_RS15805 0.50 -1.4 1,311,155 - BBR_RS15805 0.52 -1.1 1,311,166 - BBR_RS15805 0.53 +0.5 1,311,215 - BBR_RS15805 0.58 -0.6 1,311,407 - BBR_RS15805 0.78 -0.2 1,311,445 - BBR_RS15805 0.82 +0.4 1,311,503 + BBR_RS15805 0.88 -1.3 1,311,545 + -0.9 1,311,691 + -0.2 1,311,691 + -0.6 1,311,716 - +0.8 1,311,718 - +0.6 1,311,726 + +0.8 1,311,739 + -0.4 1,311,741 - -0.4 1,311,741 - -0.2 1,311,844 + -0.6 1,311,853 - -0.2 1,311,877 + -1.0 1,311,899 - -0.0 1,311,910 - +0.1 1,311,921 - +1.0 1,311,930 + -0.9 1,311,930 + +1.3 1,311,952 + BBR_RS15810 0.12 -1.9 1,311,952 + BBR_RS15810 0.12 +0.7 1,311,954 + BBR_RS15810 0.12 -0.5 1,311,960 - BBR_RS15810 0.13 +0.6 1,311,960 - BBR_RS15810 0.13 +0.0 1,311,962 - BBR_RS15810 0.13 +0.2 1,311,972 - BBR_RS15810 0.14 +0.1 1,312,021 - BBR_RS15810 0.20 +0.8 1,312,061 + BBR_RS15810 0.24 +0.3 1,312,061 + BBR_RS15810 0.24 +0.4 1,312,069 - BBR_RS15810 0.25 +0.2 1,312,069 - BBR_RS15810 0.25 -0.7 1,312,093 - BBR_RS15810 0.28 +0.5 1,312,097 + BBR_RS15810 0.29 +0.2 1,312,098 + BBR_RS15810 0.29 -0.2 1,312,176 + BBR_RS15810 0.38 -1.4 1,312,184 - BBR_RS15810 0.39 +0.9 1,312,184 - BBR_RS15810 0.39 +0.2 1,312,198 + BBR_RS15810 0.40 +0.4 1,312,206 - BBR_RS15810 0.41 -0.1 1,312,206 - BBR_RS15810 0.41 -0.3 1,312,206 - BBR_RS15810 0.41 +0.5 1,312,206 - BBR_RS15810 0.41 +0.3 1,312,211 - BBR_RS15810 0.42 -0.1 1,312,253 - BBR_RS15810 0.47 -0.4 1,312,349 - BBR_RS15810 0.58 -0.4 1,312,357 + BBR_RS15810 0.59 +0.4 1,312,448 - BBR_RS15810 0.69 +0.6 1,312,484 + BBR_RS15810 0.73 +0.3 1,312,495 + BBR_RS15810 0.74 -1.2 1,312,500 + BBR_RS15810 0.75 +0.1 1,312,500 + BBR_RS15810 0.75 -0.3 1,312,503 - BBR_RS15810 0.75 +0.5 1,312,508 - BBR_RS15810 0.76 -0.1 1,312,508 - BBR_RS15810 0.76 +0.2 1,312,578 - BBR_RS15810 0.84 +0.3 1,312,578 - BBR_RS15810 0.84 +0.2 1,312,595 - BBR_RS15810 0.86 -1.0
Or see this region's nucleotide sequence