Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15205 and BBR_RS15210 are separated by 223 nucleotides BBR_RS15210 and BBR_RS15215 are separated by 198 nucleotides
BBR_RS15205: BBR_RS15205 - 50S ribosomal protein L25, at 1,180,009 to 1,180,638
_RS15205
BBR_RS15210: BBR_RS15210 - branched-chain amino acid aminotransferase, at 1,180,862 to 1,181,989
_RS15210
BBR_RS15215: BBR_RS15215 - MazG-like protein, at 1,182,188 to 1,183,069
_RS15215
Position (kb)
1180
1181
1182 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1180.242 kb on + strand, within BBR_RS15205 at 1180.334 kb on + strand, within BBR_RS15205 at 1180.580 kb on + strand at 1180.757 kb on + strand at 1180.757 kb on + strand at 1180.757 kb on + strand at 1180.785 kb on - strand at 1180.815 kb on - strand at 1180.920 kb on + strand at 1180.920 kb on + strand at 1180.939 kb on + strand at 1181.074 kb on + strand, within BBR_RS15210 at 1181.104 kb on + strand, within BBR_RS15210 at 1181.129 kb on - strand, within BBR_RS15210 at 1181.131 kb on + strand, within BBR_RS15210 at 1181.131 kb on + strand, within BBR_RS15210 at 1181.131 kb on + strand, within BBR_RS15210 at 1181.131 kb on + strand, within BBR_RS15210 at 1181.139 kb on - strand, within BBR_RS15210 at 1181.139 kb on - strand, within BBR_RS15210 at 1181.139 kb on - strand, within BBR_RS15210 at 1181.173 kb on - strand, within BBR_RS15210 at 1181.238 kb on + strand, within BBR_RS15210 at 1181.272 kb on + strand, within BBR_RS15210 at 1181.272 kb on + strand, within BBR_RS15210 at 1181.318 kb on + strand, within BBR_RS15210 at 1181.346 kb on - strand, within BBR_RS15210 at 1181.381 kb on + strand, within BBR_RS15210 at 1181.411 kb on + strand, within BBR_RS15210 at 1181.523 kb on + strand, within BBR_RS15210 at 1181.539 kb on + strand, within BBR_RS15210 at 1181.539 kb on + strand, within BBR_RS15210 at 1181.539 kb on + strand, within BBR_RS15210 at 1181.547 kb on - strand, within BBR_RS15210 at 1181.547 kb on - strand, within BBR_RS15210 at 1181.547 kb on - strand, within BBR_RS15210 at 1181.547 kb on - strand, within BBR_RS15210 at 1181.577 kb on - strand, within BBR_RS15210 at 1181.626 kb on + strand, within BBR_RS15210 at 1181.626 kb on + strand, within BBR_RS15210 at 1181.636 kb on + strand, within BBR_RS15210 at 1181.636 kb on + strand, within BBR_RS15210 at 1181.725 kb on + strand, within BBR_RS15210 at 1181.744 kb on + strand, within BBR_RS15210 at 1181.744 kb on + strand, within BBR_RS15210 at 1181.790 kb on - strand, within BBR_RS15210 at 1181.907 kb on + strand at 1181.948 kb on - strand at 1181.948 kb on - strand at 1181.958 kb on - strand at 1182.009 kb on + strand at 1182.019 kb on + strand at 1182.135 kb on + strand at 1182.145 kb on + strand at 1182.148 kb on - strand at 1182.196 kb on + strand at 1182.217 kb on + strand at 1182.239 kb on - strand at 1182.254 kb on - strand at 1182.277 kb on + strand, within BBR_RS15215 at 1182.301 kb on - strand, within BBR_RS15215 at 1182.304 kb on + strand, within BBR_RS15215 at 1182.304 kb on + strand, within BBR_RS15215 at 1182.312 kb on - strand, within BBR_RS15215 at 1182.312 kb on - strand, within BBR_RS15215 at 1182.312 kb on - strand, within BBR_RS15215 at 1182.428 kb on + strand, within BBR_RS15215 at 1182.512 kb on + strand, within BBR_RS15215 at 1182.528 kb on + strand, within BBR_RS15215 at 1182.528 kb on + strand, within BBR_RS15215 at 1182.536 kb on - strand, within BBR_RS15215 at 1182.728 kb on - strand, within BBR_RS15215 at 1182.744 kb on + strand, within BBR_RS15215 at 1182.750 kb on - strand, within BBR_RS15215 at 1182.794 kb on - strand, within BBR_RS15215 at 1182.830 kb on - strand, within BBR_RS15215 at 1182.858 kb on + strand, within BBR_RS15215 at 1182.866 kb on - strand, within BBR_RS15215 at 1182.866 kb on - strand, within BBR_RS15215 at 1182.927 kb on - strand, within BBR_RS15215 at 1182.956 kb on + strand, within BBR_RS15215
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 1,180,242 + BBR_RS15205 0.37 +1.3 1,180,334 + BBR_RS15205 0.52 +2.3 1,180,580 + -0.9 1,180,757 + -0.3 1,180,757 + +0.8 1,180,757 + -0.9 1,180,785 - -0.9 1,180,815 - -1.0 1,180,920 + -0.4 1,180,920 + -0.6 1,180,939 + +0.1 1,181,074 + BBR_RS15210 0.19 -0.7 1,181,104 + BBR_RS15210 0.21 -2.6 1,181,129 - BBR_RS15210 0.24 +0.1 1,181,131 + BBR_RS15210 0.24 +0.0 1,181,131 + BBR_RS15210 0.24 -0.7 1,181,131 + BBR_RS15210 0.24 -0.1 1,181,131 + BBR_RS15210 0.24 -0.2 1,181,139 - BBR_RS15210 0.25 +0.3 1,181,139 - BBR_RS15210 0.25 -0.9 1,181,139 - BBR_RS15210 0.25 -0.1 1,181,173 - BBR_RS15210 0.28 +0.0 1,181,238 + BBR_RS15210 0.33 -0.7 1,181,272 + BBR_RS15210 0.36 -0.8 1,181,272 + BBR_RS15210 0.36 -0.4 1,181,318 + BBR_RS15210 0.40 -0.2 1,181,346 - BBR_RS15210 0.43 +1.0 1,181,381 + BBR_RS15210 0.46 +1.1 1,181,411 + BBR_RS15210 0.49 -0.5 1,181,523 + BBR_RS15210 0.59 +0.2 1,181,539 + BBR_RS15210 0.60 -0.5 1,181,539 + BBR_RS15210 0.60 +0.5 1,181,539 + BBR_RS15210 0.60 -0.5 1,181,547 - BBR_RS15210 0.61 +0.3 1,181,547 - BBR_RS15210 0.61 -1.0 1,181,547 - BBR_RS15210 0.61 -0.1 1,181,547 - BBR_RS15210 0.61 +0.0 1,181,577 - BBR_RS15210 0.63 +1.3 1,181,626 + BBR_RS15210 0.68 -2.8 1,181,626 + BBR_RS15210 0.68 -0.9 1,181,636 + BBR_RS15210 0.69 -1.5 1,181,636 + BBR_RS15210 0.69 -2.0 1,181,725 + BBR_RS15210 0.77 -1.7 1,181,744 + BBR_RS15210 0.78 -1.6 1,181,744 + BBR_RS15210 0.78 -3.1 1,181,790 - BBR_RS15210 0.82 -1.6 1,181,907 + -0.5 1,181,948 - -2.0 1,181,948 - +0.2 1,181,958 - +1.3 1,182,009 + -0.3 1,182,019 + -0.4 1,182,135 + -0.8 1,182,145 + -1.0 1,182,148 - -0.7 1,182,196 + +0.5 1,182,217 + +0.1 1,182,239 - -1.1 1,182,254 - +0.2 1,182,277 + BBR_RS15215 0.10 -0.6 1,182,301 - BBR_RS15215 0.13 +0.8 1,182,304 + BBR_RS15215 0.13 +1.3 1,182,304 + BBR_RS15215 0.13 -0.1 1,182,312 - BBR_RS15215 0.14 +0.9 1,182,312 - BBR_RS15215 0.14 +0.5 1,182,312 - BBR_RS15215 0.14 +0.0 1,182,428 + BBR_RS15215 0.27 -0.6 1,182,512 + BBR_RS15215 0.37 -0.4 1,182,528 + BBR_RS15215 0.39 +1.2 1,182,528 + BBR_RS15215 0.39 +0.8 1,182,536 - BBR_RS15215 0.39 -1.9 1,182,728 - BBR_RS15215 0.61 +0.1 1,182,744 + BBR_RS15215 0.63 -0.1 1,182,750 - BBR_RS15215 0.64 +0.1 1,182,794 - BBR_RS15215 0.69 +0.4 1,182,830 - BBR_RS15215 0.73 -1.1 1,182,858 + BBR_RS15215 0.76 +0.4 1,182,866 - BBR_RS15215 0.77 +0.5 1,182,866 - BBR_RS15215 0.77 +0.3 1,182,927 - BBR_RS15215 0.84 +0.6 1,182,956 + BBR_RS15215 0.87 +0.5
Or see this region's nucleotide sequence