Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15175 and BBR_RS15180 are separated by 73 nucleotides BBR_RS15180 and BBR_RS15185 are separated by 392 nucleotides
BBR_RS15175: BBR_RS15175 - GTPase Era, at 1,171,806 to 1,172,813
_RS15175
BBR_RS15180: BBR_RS15180 - long-chain fatty acid--CoA ligase, at 1,172,887 to 1,174,920
_RS15180
BBR_RS15185: BBR_RS15185 - NAD(P) transhydrogenase subunit alpha, at 1,175,313 to 1,176,476
_RS15185
Position (kb)
1172
1173
1174
1175 Strain fitness (log2 ratio)
-2
-1
0
1 at 1172.920 kb on - strand at 1172.950 kb on - strand at 1173.166 kb on - strand, within BBR_RS15180 at 1173.194 kb on - strand, within BBR_RS15180 at 1173.199 kb on - strand, within BBR_RS15180 at 1173.266 kb on - strand, within BBR_RS15180 at 1173.331 kb on - strand, within BBR_RS15180 at 1173.454 kb on - strand, within BBR_RS15180 at 1173.563 kb on - strand, within BBR_RS15180 at 1173.575 kb on + strand, within BBR_RS15180 at 1173.734 kb on - strand, within BBR_RS15180 at 1173.741 kb on - strand, within BBR_RS15180 at 1173.741 kb on - strand, within BBR_RS15180 at 1173.753 kb on - strand, within BBR_RS15180 at 1173.755 kb on - strand, within BBR_RS15180 at 1173.776 kb on - strand, within BBR_RS15180 at 1174.036 kb on - strand, within BBR_RS15180 at 1174.079 kb on - strand, within BBR_RS15180 at 1174.103 kb on - strand, within BBR_RS15180 at 1174.149 kb on - strand, within BBR_RS15180 at 1174.149 kb on - strand, within BBR_RS15180 at 1174.149 kb on - strand, within BBR_RS15180 at 1174.159 kb on - strand, within BBR_RS15180 at 1174.226 kb on + strand, within BBR_RS15180 at 1174.228 kb on - strand, within BBR_RS15180 at 1174.244 kb on - strand, within BBR_RS15180 at 1174.432 kb on - strand, within BBR_RS15180 at 1174.453 kb on + strand, within BBR_RS15180 at 1174.515 kb on - strand, within BBR_RS15180 at 1174.518 kb on - strand, within BBR_RS15180 at 1174.518 kb on - strand, within BBR_RS15180 at 1174.593 kb on - strand, within BBR_RS15180 at 1174.610 kb on - strand, within BBR_RS15180 at 1174.657 kb on - strand, within BBR_RS15180 at 1174.688 kb on - strand, within BBR_RS15180 at 1174.836 kb on - strand at 1174.904 kb on + strand at 1174.977 kb on - strand at 1175.048 kb on + strand at 1175.139 kb on + strand at 1175.139 kb on + strand at 1175.147 kb on - strand at 1175.152 kb on - strand at 1175.342 kb on + strand at 1175.427 kb on - strand at 1175.523 kb on + strand, within BBR_RS15185 at 1175.579 kb on + strand, within BBR_RS15185 at 1175.585 kb on + strand, within BBR_RS15185 at 1175.585 kb on + strand, within BBR_RS15185 at 1175.598 kb on - strand, within BBR_RS15185 at 1175.604 kb on - strand, within BBR_RS15185 at 1175.623 kb on + strand, within BBR_RS15185 at 1175.636 kb on + strand, within BBR_RS15185 at 1175.660 kb on + strand, within BBR_RS15185 at 1175.660 kb on + strand, within BBR_RS15185 at 1175.677 kb on - strand, within BBR_RS15185 at 1175.724 kb on + strand, within BBR_RS15185 at 1175.749 kb on - strand, within BBR_RS15185 at 1175.813 kb on - strand, within BBR_RS15185 at 1175.838 kb on + strand, within BBR_RS15185 at 1175.845 kb on - strand, within BBR_RS15185 at 1175.850 kb on - strand, within BBR_RS15185 at 1175.917 kb on + strand, within BBR_RS15185 at 1175.917 kb on - strand, within BBR_RS15185
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 1,172,920 - -0.8 1,172,950 - -1.1 1,173,166 - BBR_RS15180 0.14 -0.2 1,173,194 - BBR_RS15180 0.15 +0.5 1,173,199 - BBR_RS15180 0.15 -0.0 1,173,266 - BBR_RS15180 0.19 -2.4 1,173,331 - BBR_RS15180 0.22 -0.8 1,173,454 - BBR_RS15180 0.28 -0.4 1,173,563 - BBR_RS15180 0.33 -0.1 1,173,575 + BBR_RS15180 0.34 -0.1 1,173,734 - BBR_RS15180 0.42 -0.6 1,173,741 - BBR_RS15180 0.42 +1.3 1,173,741 - BBR_RS15180 0.42 -0.7 1,173,753 - BBR_RS15180 0.43 -0.5 1,173,755 - BBR_RS15180 0.43 +0.0 1,173,776 - BBR_RS15180 0.44 -2.3 1,174,036 - BBR_RS15180 0.56 -0.2 1,174,079 - BBR_RS15180 0.59 -1.2 1,174,103 - BBR_RS15180 0.60 -1.4 1,174,149 - BBR_RS15180 0.62 -1.1 1,174,149 - BBR_RS15180 0.62 +0.2 1,174,149 - BBR_RS15180 0.62 -0.2 1,174,159 - BBR_RS15180 0.63 -0.7 1,174,226 + BBR_RS15180 0.66 -0.2 1,174,228 - BBR_RS15180 0.66 -0.4 1,174,244 - BBR_RS15180 0.67 -1.9 1,174,432 - BBR_RS15180 0.76 -1.6 1,174,453 + BBR_RS15180 0.77 +0.0 1,174,515 - BBR_RS15180 0.80 -0.1 1,174,518 - BBR_RS15180 0.80 -2.1 1,174,518 - BBR_RS15180 0.80 -2.1 1,174,593 - BBR_RS15180 0.84 -0.5 1,174,610 - BBR_RS15180 0.85 +0.3 1,174,657 - BBR_RS15180 0.87 +0.8 1,174,688 - BBR_RS15180 0.89 -0.1 1,174,836 - -0.7 1,174,904 + +0.5 1,174,977 - +0.4 1,175,048 + +0.7 1,175,139 + -0.9 1,175,139 + -0.7 1,175,147 - -0.4 1,175,152 - -1.1 1,175,342 + +0.0 1,175,427 - -0.7 1,175,523 + BBR_RS15185 0.18 -0.7 1,175,579 + BBR_RS15185 0.23 -0.6 1,175,585 + BBR_RS15185 0.23 +0.9 1,175,585 + BBR_RS15185 0.23 -0.3 1,175,598 - BBR_RS15185 0.24 -1.1 1,175,604 - BBR_RS15185 0.25 +0.7 1,175,623 + BBR_RS15185 0.27 -0.1 1,175,636 + BBR_RS15185 0.28 -0.7 1,175,660 + BBR_RS15185 0.30 +0.5 1,175,660 + BBR_RS15185 0.30 -0.7 1,175,677 - BBR_RS15185 0.31 +0.6 1,175,724 + BBR_RS15185 0.35 +1.0 1,175,749 - BBR_RS15185 0.37 +0.8 1,175,813 - BBR_RS15185 0.43 +0.8 1,175,838 + BBR_RS15185 0.45 +0.2 1,175,845 - BBR_RS15185 0.46 +1.0 1,175,850 - BBR_RS15185 0.46 -0.5 1,175,917 + BBR_RS15185 0.52 -0.4 1,175,917 - BBR_RS15185 0.52 +0.8
Or see this region's nucleotide sequence