Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15060 and BBR_RS15065 are separated by 8 nucleotides BBR_RS15065 and BBR_RS15070 are separated by 12 nucleotides BBR_RS15070 and BBR_RS15075 are separated by 99 nucleotides BBR_RS15075 and BBR_RS15080 are separated by 48 nucleotides
BBR_RS15060: BBR_RS15060 - alanine--tRNA ligase, at 1,146,359 to 1,149,037
_RS15060
BBR_RS15065: BBR_RS15065 - Holliday junction resolvase RuvX, at 1,149,046 to 1,149,498
_RS15065
BBR_RS15070: BBR_RS15070 - endolytic transglycosylase MltG, at 1,149,511 to 1,150,698
_RS15070
BBR_RS15075: BBR_RS15075 - peptidase A24, at 1,150,798 to 1,151,271
_RS15075
BBR_RS15080: BBR_RS15080 - chorismate synthase, at 1,151,320 to 1,152,507
_RS15080
Position (kb)
1149
1150
1151 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1149.011 kb on + strand at 1149.036 kb on + strand at 1149.036 kb on + strand at 1149.041 kb on + strand at 1149.041 kb on + strand at 1149.472 kb on + strand at 1149.472 kb on + strand at 1149.472 kb on + strand at 1149.662 kb on - strand, within BBR_RS15070 at 1149.671 kb on + strand, within BBR_RS15070 at 1150.082 kb on - strand, within BBR_RS15070 at 1150.087 kb on - strand, within BBR_RS15070 at 1150.162 kb on - strand, within BBR_RS15070 at 1150.218 kb on + strand, within BBR_RS15070 at 1150.301 kb on - strand, within BBR_RS15070 at 1150.361 kb on - strand, within BBR_RS15070 at 1150.386 kb on + strand, within BBR_RS15070 at 1150.482 kb on + strand, within BBR_RS15070 at 1150.600 kb on + strand at 1150.690 kb on + strand at 1150.698 kb on + strand at 1150.716 kb on + strand at 1150.716 kb on + strand at 1150.719 kb on - strand at 1150.726 kb on + strand at 1150.747 kb on - strand at 1150.755 kb on + strand at 1150.764 kb on - strand at 1150.765 kb on - strand at 1150.790 kb on - strand at 1150.896 kb on + strand, within BBR_RS15075 at 1150.904 kb on - strand, within BBR_RS15075 at 1150.928 kb on + strand, within BBR_RS15075 at 1150.940 kb on + strand, within BBR_RS15075 at 1150.948 kb on - strand, within BBR_RS15075 at 1150.972 kb on - strand, within BBR_RS15075 at 1150.972 kb on - strand, within BBR_RS15075 at 1150.996 kb on + strand, within BBR_RS15075 at 1151.067 kb on + strand, within BBR_RS15075 at 1151.085 kb on - strand, within BBR_RS15075 at 1151.085 kb on - strand, within BBR_RS15075 at 1151.094 kb on - strand, within BBR_RS15075 at 1151.095 kb on - strand, within BBR_RS15075 at 1151.097 kb on - strand, within BBR_RS15075 at 1151.101 kb on + strand, within BBR_RS15075 at 1151.107 kb on - strand, within BBR_RS15075 at 1151.107 kb on - strand, within BBR_RS15075 at 1151.107 kb on - strand, within BBR_RS15075 at 1151.107 kb on - strand, within BBR_RS15075 at 1151.133 kb on + strand, within BBR_RS15075 at 1151.187 kb on - strand, within BBR_RS15075 at 1151.193 kb on + strand, within BBR_RS15075 at 1151.193 kb on + strand, within BBR_RS15075 at 1151.206 kb on - strand, within BBR_RS15075 at 1151.229 kb on - strand at 1151.229 kb on - strand at 1151.234 kb on - strand at 1151.234 kb on - strand at 1151.234 kb on - strand at 1151.262 kb on - strand at 1151.279 kb on + strand at 1151.435 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 1,149,011 + -0.4 1,149,036 + -1.6 1,149,036 + +0.2 1,149,041 + -0.5 1,149,041 + -0.1 1,149,472 + -1.4 1,149,472 + -0.1 1,149,472 + -0.5 1,149,662 - BBR_RS15070 0.13 -1.5 1,149,671 + BBR_RS15070 0.13 +0.5 1,150,082 - BBR_RS15070 0.48 -1.7 1,150,087 - BBR_RS15070 0.48 +1.8 1,150,162 - BBR_RS15070 0.55 -0.8 1,150,218 + BBR_RS15070 0.60 -1.6 1,150,301 - BBR_RS15070 0.66 -0.7 1,150,361 - BBR_RS15070 0.72 +0.3 1,150,386 + BBR_RS15070 0.74 -1.5 1,150,482 + BBR_RS15070 0.82 -0.3 1,150,600 + -0.5 1,150,690 + -0.0 1,150,698 + -0.5 1,150,716 + -1.6 1,150,716 + +0.1 1,150,719 - -0.3 1,150,726 + +0.3 1,150,747 - +0.9 1,150,755 + -0.5 1,150,764 - +0.9 1,150,765 - -0.5 1,150,790 - +0.4 1,150,896 + BBR_RS15075 0.21 -0.2 1,150,904 - BBR_RS15075 0.22 +0.8 1,150,928 + BBR_RS15075 0.27 -0.3 1,150,940 + BBR_RS15075 0.30 +0.8 1,150,948 - BBR_RS15075 0.32 -0.5 1,150,972 - BBR_RS15075 0.37 +0.9 1,150,972 - BBR_RS15075 0.37 -0.2 1,150,996 + BBR_RS15075 0.42 +0.8 1,151,067 + BBR_RS15075 0.57 -1.1 1,151,085 - BBR_RS15075 0.61 +0.8 1,151,085 - BBR_RS15075 0.61 -0.0 1,151,094 - BBR_RS15075 0.62 +0.7 1,151,095 - BBR_RS15075 0.63 +0.8 1,151,097 - BBR_RS15075 0.63 +0.0 1,151,101 + BBR_RS15075 0.64 +0.9 1,151,107 - BBR_RS15075 0.65 -0.7 1,151,107 - BBR_RS15075 0.65 -0.2 1,151,107 - BBR_RS15075 0.65 +0.5 1,151,107 - BBR_RS15075 0.65 -0.1 1,151,133 + BBR_RS15075 0.71 -0.5 1,151,187 - BBR_RS15075 0.82 -0.3 1,151,193 + BBR_RS15075 0.83 -0.2 1,151,193 + BBR_RS15075 0.83 -0.5 1,151,206 - BBR_RS15075 0.86 -0.7 1,151,229 - +0.4 1,151,229 - -1.2 1,151,234 - +0.5 1,151,234 - +1.2 1,151,234 - -0.2 1,151,262 - +0.3 1,151,279 + -3.1 1,151,435 + -1.2
Or see this region's nucleotide sequence