Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14770 and BBR_RS14775 are separated by 174 nucleotides BBR_RS14775 and BBR_RS14780 are separated by 21 nucleotides BBR_RS14780 and BBR_RS20860 are separated by 199 nucleotides BBR_RS20860 and BBR_RS14790 are separated by 10 nucleotides
BBR_RS14770: BBR_RS14770 - hypothetical protein, at 1,088,757 to 1,089,920
_RS14770
BBR_RS14775: BBR_RS14775 - hypothetical protein, at 1,090,095 to 1,090,808
_RS14775
BBR_RS14780: BBR_RS14780 - XRE family transcriptional regulator, at 1,090,830 to 1,091,384
_RS14780
BBR_RS20860: BBR_RS20860 - hypothetical protein, at 1,091,584 to 1,092,025
_RS20860
BBR_RS14790: BBR_RS14790 - phosphorylase, at 1,092,036 to 1,093,373
_RS14790
Position (kb)
1090
1091
1092 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1089.997 kb on + strand at 1090.005 kb on - strand at 1090.090 kb on + strand at 1090.095 kb on + strand at 1090.124 kb on + strand at 1090.154 kb on - strand at 1090.176 kb on + strand, within BBR_RS14775 at 1090.184 kb on - strand, within BBR_RS14775 at 1090.202 kb on + strand, within BBR_RS14775 at 1090.312 kb on - strand, within BBR_RS14775 at 1090.367 kb on + strand, within BBR_RS14775 at 1090.375 kb on - strand, within BBR_RS14775 at 1090.384 kb on + strand, within BBR_RS14775 at 1090.409 kb on + strand, within BBR_RS14775 at 1090.414 kb on + strand, within BBR_RS14775 at 1090.484 kb on + strand, within BBR_RS14775 at 1090.502 kb on - strand, within BBR_RS14775 at 1090.607 kb on - strand, within BBR_RS14775 at 1090.624 kb on - strand, within BBR_RS14775 at 1090.706 kb on + strand, within BBR_RS14775 at 1090.737 kb on - strand at 1090.797 kb on + strand at 1090.924 kb on - strand, within BBR_RS14780 at 1090.939 kb on - strand, within BBR_RS14780 at 1090.977 kb on + strand, within BBR_RS14780 at 1091.044 kb on + strand, within BBR_RS14780 at 1091.087 kb on - strand, within BBR_RS14780 at 1091.087 kb on - strand, within BBR_RS14780 at 1091.109 kb on + strand, within BBR_RS14780 at 1091.117 kb on + strand, within BBR_RS14780 at 1091.125 kb on - strand, within BBR_RS14780 at 1091.165 kb on + strand, within BBR_RS14780 at 1091.193 kb on - strand, within BBR_RS14780 at 1091.299 kb on - strand, within BBR_RS14780 at 1091.335 kb on - strand at 1091.384 kb on - strand at 1091.482 kb on + strand at 1091.515 kb on + strand at 1091.554 kb on - strand at 1091.573 kb on + strand at 1091.798 kb on + strand, within BBR_RS20860 at 1091.806 kb on + strand, within BBR_RS20860 at 1091.808 kb on + strand, within BBR_RS20860 at 1091.838 kb on - strand, within BBR_RS20860 at 1091.908 kb on + strand, within BBR_RS20860 at 1091.916 kb on + strand, within BBR_RS20860 at 1091.975 kb on - strand, within BBR_RS20860 at 1092.180 kb on - strand, within BBR_RS14790 at 1092.180 kb on - strand, within BBR_RS14790 at 1092.239 kb on + strand, within BBR_RS14790 at 1092.271 kb on - strand, within BBR_RS14790 at 1092.292 kb on + strand, within BBR_RS14790 at 1092.314 kb on - strand, within BBR_RS14790 at 1092.381 kb on - strand, within BBR_RS14790
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 1,089,997 + +0.3 1,090,005 - +0.6 1,090,090 + +1.2 1,090,095 + +0.2 1,090,124 + +2.0 1,090,154 - +3.0 1,090,176 + BBR_RS14775 0.11 -0.8 1,090,184 - BBR_RS14775 0.12 +0.1 1,090,202 + BBR_RS14775 0.15 +0.8 1,090,312 - BBR_RS14775 0.30 -0.7 1,090,367 + BBR_RS14775 0.38 -0.6 1,090,375 - BBR_RS14775 0.39 +0.4 1,090,384 + BBR_RS14775 0.40 +0.2 1,090,409 + BBR_RS14775 0.44 -0.9 1,090,414 + BBR_RS14775 0.45 -0.5 1,090,484 + BBR_RS14775 0.54 -0.1 1,090,502 - BBR_RS14775 0.57 -0.1 1,090,607 - BBR_RS14775 0.72 +0.3 1,090,624 - BBR_RS14775 0.74 +0.1 1,090,706 + BBR_RS14775 0.86 -0.2 1,090,737 - +0.5 1,090,797 + +0.0 1,090,924 - BBR_RS14780 0.17 +0.6 1,090,939 - BBR_RS14780 0.20 +0.1 1,090,977 + BBR_RS14780 0.26 -0.3 1,091,044 + BBR_RS14780 0.39 +0.8 1,091,087 - BBR_RS14780 0.46 -0.3 1,091,087 - BBR_RS14780 0.46 -1.0 1,091,109 + BBR_RS14780 0.50 -0.8 1,091,117 + BBR_RS14780 0.52 -0.3 1,091,125 - BBR_RS14780 0.53 -2.5 1,091,165 + BBR_RS14780 0.60 -0.9 1,091,193 - BBR_RS14780 0.65 -0.5 1,091,299 - BBR_RS14780 0.85 -0.4 1,091,335 - -0.1 1,091,384 - -0.8 1,091,482 + +0.2 1,091,515 + +0.9 1,091,554 - -0.4 1,091,573 + -0.3 1,091,798 + BBR_RS20860 0.48 +0.4 1,091,806 + BBR_RS20860 0.50 -0.7 1,091,808 + BBR_RS20860 0.51 -0.5 1,091,838 - BBR_RS20860 0.57 -0.3 1,091,908 + BBR_RS20860 0.73 +0.6 1,091,916 + BBR_RS20860 0.75 +0.0 1,091,975 - BBR_RS20860 0.88 +0.5 1,092,180 - BBR_RS14790 0.11 +0.1 1,092,180 - BBR_RS14790 0.11 -0.3 1,092,239 + BBR_RS14790 0.15 -0.9 1,092,271 - BBR_RS14790 0.18 -1.5 1,092,292 + BBR_RS14790 0.19 +0.0 1,092,314 - BBR_RS14790 0.21 +0.8 1,092,381 - BBR_RS14790 0.26 +0.4
Or see this region's nucleotide sequence