Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14710 and BBR_RS14715 are separated by 265 nucleotides BBR_RS14715 and BBR_RS14720 are separated by 183 nucleotides
BBR_RS14710: BBR_RS14710 - hypothetical protein, at 1,074,165 to 1,075,421
_RS14710
BBR_RS14715: BBR_RS14715 - XRE family transcriptional regulator, at 1,075,687 to 1,075,865
_RS14715
BBR_RS14720: BBR_RS14720 - MFS transporter, at 1,076,049 to 1,077,320
_RS14720
Position (kb)
1075
1076 Strain fitness (log2 ratio)
-2
-1
0
1 at 1074.751 kb on + strand, within BBR_RS14710 at 1074.859 kb on - strand, within BBR_RS14710 at 1074.896 kb on - strand, within BBR_RS14710 at 1074.917 kb on - strand, within BBR_RS14710 at 1075.196 kb on + strand, within BBR_RS14710 at 1075.252 kb on + strand, within BBR_RS14710 at 1075.363 kb on - strand at 1075.385 kb on + strand at 1075.464 kb on - strand at 1075.471 kb on + strand at 1075.517 kb on - strand at 1075.798 kb on - strand, within BBR_RS14715 at 1075.809 kb on + strand, within BBR_RS14715 at 1075.853 kb on + strand at 1075.915 kb on - strand at 1075.966 kb on + strand at 1076.010 kb on + strand at 1076.047 kb on + strand at 1076.085 kb on - strand at 1076.102 kb on - strand at 1076.102 kb on - strand at 1076.117 kb on + strand at 1076.140 kb on + strand at 1076.226 kb on + strand, within BBR_RS14720 at 1076.268 kb on - strand, within BBR_RS14720 at 1076.268 kb on - strand, within BBR_RS14720 at 1076.294 kb on - strand, within BBR_RS14720 at 1076.368 kb on - strand, within BBR_RS14720 at 1076.486 kb on + strand, within BBR_RS14720 at 1076.486 kb on + strand, within BBR_RS14720 at 1076.493 kb on + strand, within BBR_RS14720 at 1076.494 kb on - strand, within BBR_RS14720 at 1076.494 kb on - strand, within BBR_RS14720 at 1076.514 kb on + strand, within BBR_RS14720 at 1076.514 kb on + strand, within BBR_RS14720 at 1076.514 kb on + strand, within BBR_RS14720 at 1076.514 kb on + strand, within BBR_RS14720 at 1076.522 kb on - strand, within BBR_RS14720 at 1076.531 kb on - strand, within BBR_RS14720 at 1076.541 kb on - strand, within BBR_RS14720 at 1076.560 kb on - strand, within BBR_RS14720 at 1076.560 kb on - strand, within BBR_RS14720 at 1076.575 kb on - strand, within BBR_RS14720 at 1076.597 kb on - strand, within BBR_RS14720 at 1076.821 kb on + strand, within BBR_RS14720
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 1,074,751 + BBR_RS14710 0.47 +1.1 1,074,859 - BBR_RS14710 0.55 -0.5 1,074,896 - BBR_RS14710 0.58 +1.1 1,074,917 - BBR_RS14710 0.60 -0.3 1,075,196 + BBR_RS14710 0.82 -0.4 1,075,252 + BBR_RS14710 0.86 -0.1 1,075,363 - -0.1 1,075,385 + -0.2 1,075,464 - +0.1 1,075,471 + -0.2 1,075,517 - -0.8 1,075,798 - BBR_RS14715 0.62 -1.0 1,075,809 + BBR_RS14715 0.68 -0.3 1,075,853 + +0.1 1,075,915 - +0.0 1,075,966 + -0.2 1,076,010 + -0.5 1,076,047 + -0.2 1,076,085 - -0.5 1,076,102 - +0.1 1,076,102 - -1.5 1,076,117 + +0.1 1,076,140 + +0.9 1,076,226 + BBR_RS14720 0.14 -0.2 1,076,268 - BBR_RS14720 0.17 +1.0 1,076,268 - BBR_RS14720 0.17 -0.9 1,076,294 - BBR_RS14720 0.19 -0.3 1,076,368 - BBR_RS14720 0.25 -1.0 1,076,486 + BBR_RS14720 0.34 -0.9 1,076,486 + BBR_RS14720 0.34 +0.8 1,076,493 + BBR_RS14720 0.35 +0.2 1,076,494 - BBR_RS14720 0.35 -1.2 1,076,494 - BBR_RS14720 0.35 -0.2 1,076,514 + BBR_RS14720 0.37 +0.2 1,076,514 + BBR_RS14720 0.37 +0.3 1,076,514 + BBR_RS14720 0.37 +1.0 1,076,514 + BBR_RS14720 0.37 -0.1 1,076,522 - BBR_RS14720 0.37 -0.2 1,076,531 - BBR_RS14720 0.38 -0.2 1,076,541 - BBR_RS14720 0.39 -0.4 1,076,560 - BBR_RS14720 0.40 -2.2 1,076,560 - BBR_RS14720 0.40 +0.6 1,076,575 - BBR_RS14720 0.41 -0.2 1,076,597 - BBR_RS14720 0.43 +1.3 1,076,821 + BBR_RS14720 0.61 +1.1
Or see this region's nucleotide sequence