Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14575 and BBR_RS20835 are separated by 174 nucleotides BBR_RS20835 and BBR_RS14585 are separated by 7 nucleotides BBR_RS14585 and BBR_RS14590 are separated by 99 nucleotides
BBR_RS14575: BBR_RS14575 - YraN family protein, at 1,043,833 to 1,044,249
_RS14575
BBR_RS20835: BBR_RS20835 - hypothetical protein, at 1,044,424 to 1,044,884
_RS20835
BBR_RS14585: BBR_RS14585 - hypothetical protein, at 1,044,892 to 1,045,122
_RS14585
BBR_RS14590: BBR_RS14590 - pyridoxine kinase, at 1,045,222 to 1,046,094
_RS14590
Position (kb)
1044
1045
1046 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1043.927 kb on - strand, within BBR_RS14575 at 1044.018 kb on - strand, within BBR_RS14575 at 1044.019 kb on + strand, within BBR_RS14575 at 1044.051 kb on - strand, within BBR_RS14575 at 1044.139 kb on + strand, within BBR_RS14575 at 1044.149 kb on + strand, within BBR_RS14575 at 1044.149 kb on + strand, within BBR_RS14575 at 1044.217 kb on + strand at 1044.257 kb on + strand at 1044.308 kb on - strand at 1044.327 kb on + strand at 1044.327 kb on + strand at 1044.327 kb on + strand at 1044.335 kb on - strand at 1044.370 kb on - strand at 1044.464 kb on + strand at 1044.471 kb on + strand, within BBR_RS20835 at 1044.501 kb on - strand, within BBR_RS20835 at 1044.510 kb on + strand, within BBR_RS20835 at 1044.529 kb on + strand, within BBR_RS20835 at 1044.529 kb on + strand, within BBR_RS20835 at 1044.535 kb on - strand, within BBR_RS20835 at 1044.631 kb on + strand, within BBR_RS20835 at 1044.631 kb on + strand, within BBR_RS20835 at 1044.758 kb on + strand, within BBR_RS20835 at 1044.815 kb on + strand, within BBR_RS20835 at 1044.815 kb on + strand, within BBR_RS20835 at 1044.874 kb on + strand at 1044.874 kb on + strand at 1044.874 kb on + strand at 1044.874 kb on + strand at 1044.874 kb on - strand at 1044.888 kb on - strand at 1044.905 kb on + strand at 1044.905 kb on + strand at 1045.003 kb on - strand, within BBR_RS14585 at 1045.025 kb on - strand, within BBR_RS14585 at 1045.061 kb on - strand, within BBR_RS14585 at 1045.175 kb on - strand at 1045.210 kb on + strand at 1045.289 kb on + strand at 1045.354 kb on - strand, within BBR_RS14590 at 1045.364 kb on - strand, within BBR_RS14590 at 1045.386 kb on + strand, within BBR_RS14590 at 1045.394 kb on - strand, within BBR_RS14590 at 1045.498 kb on - strand, within BBR_RS14590 at 1045.562 kb on + strand, within BBR_RS14590 at 1045.562 kb on + strand, within BBR_RS14590 at 1045.685 kb on + strand, within BBR_RS14590 at 1045.693 kb on - strand, within BBR_RS14590 at 1045.693 kb on - strand, within BBR_RS14590 at 1045.695 kb on + strand, within BBR_RS14590 at 1045.695 kb on + strand, within BBR_RS14590 at 1045.695 kb on + strand, within BBR_RS14590 at 1045.703 kb on - strand, within BBR_RS14590 at 1045.703 kb on - strand, within BBR_RS14590 at 1045.712 kb on + strand, within BBR_RS14590 at 1045.740 kb on - strand, within BBR_RS14590 at 1045.740 kb on - strand, within BBR_RS14590 at 1045.745 kb on - strand, within BBR_RS14590 at 1045.756 kb on - strand, within BBR_RS14590 at 1045.803 kb on + strand, within BBR_RS14590 at 1045.811 kb on - strand, within BBR_RS14590 at 1045.813 kb on + strand, within BBR_RS14590 at 1045.837 kb on + strand, within BBR_RS14590 at 1045.850 kb on - strand, within BBR_RS14590 at 1045.906 kb on + strand, within BBR_RS14590 at 1045.919 kb on + strand, within BBR_RS14590 at 1045.931 kb on + strand, within BBR_RS14590 at 1045.931 kb on + strand, within BBR_RS14590 at 1045.944 kb on - strand, within BBR_RS14590 at 1046.056 kb on - strand at 1046.079 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 1,043,927 - BBR_RS14575 0.23 -0.5 1,044,018 - BBR_RS14575 0.44 +1.9 1,044,019 + BBR_RS14575 0.45 +0.7 1,044,051 - BBR_RS14575 0.52 +0.4 1,044,139 + BBR_RS14575 0.73 -0.2 1,044,149 + BBR_RS14575 0.76 +1.4 1,044,149 + BBR_RS14575 0.76 +0.5 1,044,217 + +0.0 1,044,257 + +0.0 1,044,308 - +0.6 1,044,327 + +0.4 1,044,327 + -0.8 1,044,327 + -0.3 1,044,335 - -0.1 1,044,370 - -0.3 1,044,464 + -2.2 1,044,471 + BBR_RS20835 0.10 -0.3 1,044,501 - BBR_RS20835 0.17 -1.2 1,044,510 + BBR_RS20835 0.19 -0.5 1,044,529 + BBR_RS20835 0.23 +0.1 1,044,529 + BBR_RS20835 0.23 +0.3 1,044,535 - BBR_RS20835 0.24 -0.5 1,044,631 + BBR_RS20835 0.45 -0.1 1,044,631 + BBR_RS20835 0.45 +0.7 1,044,758 + BBR_RS20835 0.72 +1.7 1,044,815 + BBR_RS20835 0.85 +0.3 1,044,815 + BBR_RS20835 0.85 +0.2 1,044,874 + +1.3 1,044,874 + +0.8 1,044,874 + +0.5 1,044,874 + -0.5 1,044,874 - +0.4 1,044,888 - +1.0 1,044,905 + +0.1 1,044,905 + -0.1 1,045,003 - BBR_RS14585 0.48 -1.7 1,045,025 - BBR_RS14585 0.58 -0.8 1,045,061 - BBR_RS14585 0.73 -0.5 1,045,175 - +1.9 1,045,210 + +0.5 1,045,289 + -0.5 1,045,354 - BBR_RS14590 0.15 -1.6 1,045,364 - BBR_RS14590 0.16 -0.7 1,045,386 + BBR_RS14590 0.19 +0.0 1,045,394 - BBR_RS14590 0.20 +1.5 1,045,498 - BBR_RS14590 0.32 -1.4 1,045,562 + BBR_RS14590 0.39 -0.6 1,045,562 + BBR_RS14590 0.39 +0.1 1,045,685 + BBR_RS14590 0.53 +1.9 1,045,693 - BBR_RS14590 0.54 +0.8 1,045,693 - BBR_RS14590 0.54 +0.3 1,045,695 + BBR_RS14590 0.54 -0.1 1,045,695 + BBR_RS14590 0.54 -0.1 1,045,695 + BBR_RS14590 0.54 -1.9 1,045,703 - BBR_RS14590 0.55 +0.1 1,045,703 - BBR_RS14590 0.55 -2.5 1,045,712 + BBR_RS14590 0.56 +0.5 1,045,740 - BBR_RS14590 0.59 -0.1 1,045,740 - BBR_RS14590 0.59 +0.5 1,045,745 - BBR_RS14590 0.60 +0.4 1,045,756 - BBR_RS14590 0.61 +1.1 1,045,803 + BBR_RS14590 0.67 +0.6 1,045,811 - BBR_RS14590 0.67 -1.7 1,045,813 + BBR_RS14590 0.68 +0.8 1,045,837 + BBR_RS14590 0.70 +1.0 1,045,850 - BBR_RS14590 0.72 +1.6 1,045,906 + BBR_RS14590 0.78 +0.6 1,045,919 + BBR_RS14590 0.80 +0.7 1,045,931 + BBR_RS14590 0.81 -1.0 1,045,931 + BBR_RS14590 0.81 +0.1 1,045,944 - BBR_RS14590 0.83 +0.7 1,046,056 - +0.4 1,046,079 - +1.0
Or see this region's nucleotide sequence